LOCUS Exported 7831 bp ds-DNA circular SYN 03-JAN-2013 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pcDNA5/FRT GFP-Hec1 WILCO SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7831) AUTHORS Banafsheh TITLE Direct Submission JOURNAL Exported Jul 13, 2018 from SnapGene Viewer 4.1.9 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7831 /organism="synthetic DNA construct" /note="/dnas_title=pcDNA5/FRT GFP-Hec1 WILCO" promoter 232..958 /label=CMV promoter (with TetO's) /note="/dnas_title=CMV promoter (with TetO's)" /note="/vntifkey=29" primer_bind 769..789 /label=CMV forward primer CDS 1080..1796 /codon_start=1 /label=EGFP /note="/dnas_title=EGFP" /note="/vntifkey=4" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 1812..3737 /codon_start=1 /label=Hec1 9D /note="/dnas_title=Hec1 9D" /note="/vntifkey=4" /translation="MKRDDVSDGGAGRLDMQELRSQDVNKQGLYTPQTKEKPTFGKLDI NKPDSERKVDLFGKRTDGHGSRNDQLGIFSSSEKIKDPRPLNDKAFIQQCIRQLCEFLT ENGYAHNVSMKSLQAPSVKDFLKIFTFLYGFLCPSYELPDTKFEEEVPRIFKDLGYPFA LSKSSMYTVGAPHTWPHIVAALVWLIDCIKIHTAMKESSPLFDDGQPWGEETEDGIMHN KLFLDYTIKCYESFMSGADSFDEMNAELQSKLKDLFNVDAFKLESLEAKNRALNEQIAR LEQEREKEPNRLESLRKLKASLQGDVQKYQAYMSNLESHSAILDQKLNGLNEEIARVEL ECETIKQENTRLQNIIDNQKYSVADIERINHERNELQQTINKLTKDLEAEQQKLWNEEL KYARGKEAIETQLAEYHKLARKLKLIPKGAENSKGYDFEIKFNPEAGANCLVKYRAQVY VPLKELLNETEEEINKALNKKMGLEDTLEQLNAMITESKRSVRTLKEEVQKLDDLYQQK IKEAEEEDEKCASELESLEKHKHLLESTVNQGLSEAMNELDAVQREYQLVVQTTTEERR KVGNNLQRLLEMVATHVGSVEKHLEEQIAKVDREYEECMSEDLSENIKEIRDKYEKKAT LIKSSEE" misc_recomb 4297..4344 /label=FRT /note="/dnas_title=FRT" /note="/vntifkey=86" CDS 4352..5374 /codon_start=1 /label=Hygromycin (no ATG) /note="/dnas_title=Hygromycin (no ATG)" /note="/vntifkey=4" /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE " polyA_signal 5504..5634 /label=SV40 pA /note="/dnas_title=SV40 pA" /note="/vntifkey=25" rep_origin complement(6017..6690) /direction=LEFT /label=pUC ori /note="/dnas_title=pUC ori" /note="/vntifkey=33" CDS complement(6835..7695) /codon_start=1 /label=Amp (R) /note="/dnas_title=Amp (R)" /note="/vntifkey=4" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(7696..7794) /label=bla promoter /note="/dnas_title=bla promoter" /note="/vntifkey=30" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc 841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga 901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga 961 ctctagcgtt taaacttaag cttggtaccg agctcggatc cactagtcca gtgtggtgga 1021 attctgcaga tatccagcac agtggcggcc gctcgagtct agcgctaccg gtcgccacca 1081 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg 1141 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg 1201 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc 1261 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc 1321 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct 1381 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg 1441 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca 1501 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg 1561 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg 1621 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact 1681 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc 1741 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtccg 1801 gactcagatc catgaagcgc gatgatgttt ccgacggtgg tgctggccgc ctcgacatgc 1861 aggagttaag atcccaggat gtaaataaac aaggcctcta tacccctcaa accaaagaga 1921 aaccaacctt tggaaagttg gatataaaca aaccggactc tgaaagaaaa gtcgacctat 1981 ttggcaaaag aactgatgga catggatccc ggaatgatca acttggtata ttttccagtt 2041 ctgagaaaat caaggacccg agaccactta atgacaaagc attcattcag cagtgtattc 2101 gacaactctg tgagtttctt acagaaaatg gttatgcaca taatgtgtcc atgaaatctc 2161 tacaagctcc ctctgttaaa gacttcctga agatcttcac atttctttat ggcttcctgt 2221 gcccctcata cgaacttcct gacacaaagt ttgaagaaga ggttccaaga atctttaaag 2281 accttgggta tccttttgca ctatccaaaa gctccatgta cacagtgggg gctcctcata 2341 catggcctca cattgtggca gccttagttt ggctaataga ctgcatcaag atacatactg 2401 ccatgaaaga aagctcacct ttatttgatg atgggcagcc ttggggagaa gaaactgaag 2461 atggaattat gcataataag ttgtttttgg actacaccat aaaatgctat gagagtttta 2521 tgagtggtgc cgacagcttt gatgagatga atgcagagct gcagtcaaaa ctgaaggatt 2581 tatttaatgt ggatgctttt aagctggaat cattagaagc aaaaaacaga gcattgaatg 2641 aacagattgc aagattggaa caagaaagag aaaaagagcc gaatcgtcta gagtcgttga 2701 gaaaactgaa ggcttcctta caaggagatg ttcaaaagta tcaggcatac atgagcaatt 2761 tggagtctca ttcagccatt cttgaccaga aattaaatgg tctcaatgag gaaattgcta 2821 gagtagaact agaatgtgaa acaataaaac aggagaacac tcgactacag aatatcattg 2881 acaaccagaa gtactcagtt gcagacattg agcgaataaa tcatgaaaga aatgaattgc 2941 agcagactat taataaatta accaaggacc tggaagctga acaacagaag ttgtggaatg 3001 aggagttaaa atatgccaga ggcaaagaag cgattgaaac acaattagca gagtatcaca 3061 aattggctag aaaattaaaa cttattccta aaggtgctga gaattccaaa ggttatgact 3121 ttgaaattaa gtttaatccc gaggctggtg ccaactgcct tgtcaaatac agggctcaag 3181 tttatgtacc tcttaaggaa ctcctgaatg aaactgaaga agaaattaat aaagccctaa 3241 ataaaaaaat gggtttggag gatactttag aacaattgaa tgcaatgata acagaaagca 3301 agagaagtgt gagaactctg aaagaagaag ttcaaaaatt ggatgatctt taccaacaaa 3361 aaattaagga agcagaggaa gaggatgaaa aatgtgccag tgagcttgag tccttggaga 3421 aacacaagca cctgctagaa agtactgtta accaggggct cagtgaagct atgaatgaat 3481 tagatgctgt tcagcgggaa taccaactag ttgtgcaaac cacgactgaa gaaagacgaa 3541 aagtgggaaa taacttgcaa cgtctgttag agatggttgc tacacatgtt gggtctgtag 3601 agaaacatct tgaggagcag attgctaaag ttgatagaga atatgaagaa tgcatgtcag 3661 aagatctctc ggaaaatatt aaagagatta gagataagta tgagaagaaa gctactctaa 3721 ttaagtcttc tgaagaatga ctcgaggacg gtaccgcggg cccgtttaaa cccgctgatc 3781 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc 3841 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc 3901 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg 3961 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga 4021 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt 4081 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc 4141 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca 4201 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc 4261 caaaaaactt gattagggtg atggttcacg tacctagaag ttcctattcc gaagttccta 4321 ttctctagaa agtataggaa cttccttggc caaaaagcct gaactcaccg cgacgtctgt 4381 cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg 4441 cgaagaatct cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa 4501 tagctgcgcc gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc 4561 gctcccgatt ccggaagtgc ttgacattgg ggaattcagc gagagcctga cctattgcat 4621 ctcccgccgt gcacagggtg tcacgttgca agacctgcct gaaaccgaac tgcccgctgt 4681 tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg gccgatctta gccagacgag 4741 cgggttcggc ccattcggac cgcaaggaat cggtcaatac actacatggc gtgatttcat 4801 atgcgcgatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag 4861 tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt 4921 ccggcacctc gtgcacgcgg atttcggctc caacaatgtc ctgacggaca atggccgcat 4981 aacagcggtc attgactgga gcgaggcgat gttcggggat tcccaatacg aggtcgccaa 5041 catcttcttc tggaggccgt ggttggcttg tatggagcag cagacgcgct acttcgagcg 5101 gaggcatccg gagcttgcag gatcgccgcg gctccgggcg tatatgctcc gcattggtct 5161 tgaccaactc tatcagagct tggttgacgg caatttcgat gatgcagctt gggcgcaggg 5221 tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc gggcgtacac aaatcgcccg 5281 cagaagcgcg gccgtctgga ccgatggctg tgtagaagta ctcgccgata gtggaaaccg 5341 acgccccagc actcgtccga gggcaaagga atagcacgta ctacgagatt tcgattccac 5401 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat 5461 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc 5521 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 5581 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc 5641 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg 5701 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg 5761 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc 5821 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt 5881 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 5941 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 6001 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 6061 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 6121 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 6181 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 6241 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 6301 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 6361 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 6421 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 6481 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct 6541 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 6601 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 6661 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 6721 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 6781 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 6841 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 6901 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 6961 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 7021 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 7081 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 7141 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 7201 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 7261 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 7321 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 7381 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 7441 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 7501 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 7561 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 7621 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 7681 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 7741 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 7801 cacatttccc cgaaaagtgc cacctgacgt c //