LOCUS       Exported                7220 bp ds-DNA     circular SYN 13-JUL-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7220)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 13, 2018 from SnapGene Viewer 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7220
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          4721..6649
                     /note="/dnas_title=pAceBac1-LacI-GFP-HEC1 (Sequenced)"
     gene            1341..1673
                     /label=Genta
     misc_feature    2024..2057
                     /label=LoxP
     misc_feature    2078..2482
                     /label=CMV_E
     misc_feature    2483..2688
                     /label=CMV_P
     misc_feature    2717..3844
                     /label=LacI
     misc_feature    3845..4567
                     /label=EGFP
     misc_feature    4607..4627
                     /label=TEV cleavage site
     misc_feature    4640..4684
                     /label=S peptide
     misc_feature    join(4697..4703,6668..6676)
                     /label=MCS
     misc_feature    4704..6667
                     /label=nonstandard type: insert
                     /note="/source=Corrected_pAceBAc-EGFP-LACI_Hec1(not exact 
                     cloning)"
                     /note="/type=Custom cloned insert"
     misc_feature    4721..6649
                     /label=HEC1
                     /note="HEC1"
     polyA_site      6792..6974
                     /label=SV40 late polyA
     misc_feature    7043..7054
                     /label=BstXI
ORIGIN
        1 accggttgac ttgggtcaac tgtcagacca agtttactca tatatacttt agattgattt
       61 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
      121 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
      181 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
      241 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
      301 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
      361 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
      421 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
      481 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
      541 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
      601 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
      661 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
      721 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
      781 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
      841 ctttcctgcg ttatcccctg attgacttgg gtcgctcttc ctgtggatgc gcagatgccc
      901 tgcgtaagcg ggtgtgggcg gacaataaag tcttaaactg aacaaaatag atctaaacta
      961 tgacaataaa gtcttaaact agacagaata gttgtaaact gaaatcagtc cagttatgct
     1021 gtgaaaaagc atactggact tttgttatgg ctaaagcaaa ctcttcattt tctgaagtgc
     1081 aaattgcccg tcgtattaaa gaggggcgtg gccaagggca tgtaaagact atattcgcgg
     1141 cgttgtgaca atttaccgaa caactccgcg gccgggaagc cgatctcggc ttgaacgaat
     1201 tgttaggtgg cggtacttgg gtcgatatca aagtgcatca cttcttcccg tatgcccaac
     1261 tttgtataga gagccactgc gggatcgtca ccgtaatctg cttgcacgta gatcacataa
     1321 gcaccaagcg cgttggcctc atgcttgagg agattgatga gcgcggtggc aatgccctgc
     1381 ctccggtgct cgccggagac tgcgagatca tagatataga tctcactacg cggctgctca
     1441 aacttgggca gaacgtaagc cgcgagagcg ccaacaaccg cttcttggtc gaaggcagca
     1501 agcgcgatga atgtcttact acggagcaag ttcccgaggt aatcggagtc cggctgatgt
     1561 tgggagtagg tggctacgtc tccgaactca cgaccgaaaa gatcaagagc agcccgcatg
     1621 gatttgactt ggtcagggcc gagcctacat gtgcgaatga tgcccatact tgagccacct
     1681 aactttgttt tagggcgact gccctgctgc gtaacatcgt tgctgctgcg taacatcgtt
     1741 gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct tggatgcccg
     1801 aggcatagac tgtacaaaaa aacagtcata acaagccatg aaaaccgcca ctgcgccgtt
     1861 accaccgctg cgttcggtca aggttctgga ccagttgcgt gagcgcatac gctacttgca
     1921 ttacagttta cgaaccgaac aggcttatgt caactgggtt cgtgccttca tccgtttcca
     1981 cggtgtgcgt cacccggcaa ccttgggcag cagcgaagtc gccataactt cgtatagcat
     2041 acattatacg aagttatctg taactataac ggtcctaagg tagcgagttt aaacactagt
     2101 atcgattcgc gacctactcc ggaatattaa tagtaatcaa ttacggggtc attagttcat
     2161 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     2221 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     2281 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     2341 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     2401 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     2461 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2521 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2581 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2641 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctgg tttagtgaac
     2701 cgtcagatcc gctagcatgg tgaaaccagt aacgttatac gatgtcgcag agtatgccgg
     2761 tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac
     2821 gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca
     2881 acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca
     2941 cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt
     3001 ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct
     3061 tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat
     3121 tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac
     3181 acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg tggagcatct
     3241 ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc
     3301 gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc
     3361 ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa
     3421 tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc tgggcgcaat
     3481 gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag tgggatacga
     3541 cgataccgaa gacagctcat gttatatccc gccgtcaacc accatcaaac aggattttcg
     3601 cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa
     3661 gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cgcccaatac
     3721 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     3781 ccgaagcagc ctgaggcctc ctaagaagaa gaggaaggtt gggctagcgc taccggtcgc
     3841 caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     3901 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     3961 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     4021 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     4081 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     4141 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     4201 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     4261 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     4321 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     4381 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     4441 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     4501 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     4561 gtccggaggg gatcggtgga gctccaccgg tggcggccgc tctagagaga atctttattt
     4621 tcagggcgcc gccaaattca aagaaaccgc tgctgctaaa ttcgaacgcc agcacatgga
     4681 cagcggaggt ggaggtacta gtctcgagct caagcttacc atgaagcgca gttcagtttc
     4741 cagcggtggt gctggccgcc tctccatgca ggagttaaga tcccaggatg taaataaaca
     4801 aggcctctat acccctcaaa ccaaagagaa accaaccttt ggaaagttga gtataaacaa
     4861 accgacatct gaaagaaaag tctcgctatt tggcaaaaga actagtggac atggatcacg
     4921 gaatagtcaa cttggtatat tttccagttc tgagaaaatc aaggacccga gaccacttaa
     4981 cgacaaagca ttcattcagc agtgtattcg acaactctgt gagtttctta cagaaaatgg
     5041 ttatgcacat aatgtgtcca tgaaatctct acaagctccc tctgttaaag acttcctgaa
     5101 gatcttcaca tttctttatg gcttcctgtg cccctcatac gaacttcctg acacaaagtt
     5161 tgaagaagag gttccaagaa tctttaaaga ccttgggtat ccttttgcac tatccaaaag
     5221 ctccatgtac acagtggggg ctcctcatac atggcctcac attgtggcag ccttagtttg
     5281 gctaatagac tgcatcaaga tacatactgc catgaaagaa agctcacctt tatttgatga
     5341 tgggcagcct tggggagaag aaactgaaga tggaattatg cataataagt tgtttttgga
     5401 ctacaccata aaatgctatg agagttttat gagtggtgcc gacagctttg atgagatgaa
     5461 tgcagagctg cagtcaaaac tgaaggattt atttaatgtg gatgctttta agctggaatc
     5521 attagaagca aaaaacagag cattgaatga acagattgca agattggaac aagaaagaga
     5581 aaaagaaccg aatcgtctag agtcgttgag aaaactgaag gcttccttac aaggagatgt
     5641 tcaaaagtat caggcataca tgagcaattt ggagtctcat tcagccattc ttgaccagaa
     5701 attaaatggt ctcaatgagg aaattgctag agtagaacta gaatgtgaaa caataaaaca
     5761 ggagaacact cgactacaga atatcattga caaccagaag tactcagttg cagacattga
     5821 gcgaataaat catgaaagaa atgaattgca gcagactatt aataaattaa ccaaggacct
     5881 ggaagctgaa caacagaagt tgtggaatga ggagttaaaa tatgccagag gcaaagaagc
     5941 gattgaaaca caattagcag agtatcacaa attggctaga aaattaaaac ttattcctaa
     6001 aggtgctgag aattccaaag gttatgactt tgaaattaag tttaatcccg aggctggtgc
     6061 caactgcctt gtcaaataca gggctcaagt ttatgtacct cttaaggaac tcctgaatga
     6121 aactgaagaa gaaattaata aagccctaaa taaaaaaatg ggtttggagg atactttaga
     6181 acaattgaat gcaatgataa cagaaagcaa gagaagtgtg agaactctga aagaagaagt
     6241 tcaaaagctg gatgatcttt accaacaaaa aattaaggaa gcagaggaag aggatgaaaa
     6301 atgtgccagt gagcttgagt ccttggagaa acacaagcac ctgctagaaa gtactgttaa
     6361 ccaggggctc agtgaagcta tgaatgaatt agatgctgtt cagcgggaat accaactagt
     6421 tgtgcaaacc acgactgaag aaagacgaaa agtgggaaat aacttgcaac gtctgttaga
     6481 gatggttgct acacatgttg ggtctgtaga gaaacatctt gaggagcaga ttgctaaagt
     6541 tgatagagaa tatgaagaat gcatgtcaga agatctctcg gaaaatatta aagagattag
     6601 agataagtat gagaagaaag ctactctaat taagtcttct gaagaatgac tcgacggtac
     6661 cgcgggcccg ggatccaccg gatctagata actgatcata atcagccata ccacatttgt
     6721 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     6781 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     6841 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     6901 caaactcatc aatgtatctt atcatgtctg gatctgatca ctgcttgagc ctagaagatc
     6961 cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac
     7021 tatcataacc cctagggtat acccatctaa ttggaaccag ataagtgaaa tctagttcca
     7081 aactattttg tcatttttaa ttttcgtatt agcttacgac gctacaccca gttcccatct
     7141 attttgtcac tcttccctaa ataatcctta aaaactccat ttccacccct cccagttccc
     7201 aactattttg tccgcccaca
//
