LOCUS       pT7-G-AsCas12a_c        2138 bp    DNA     circular SYN 05-JUL-2018
DEFINITION  DR274.
ACCESSION   urn.local...13-98sgkiq
KEYWORDS    .
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     misc_feature    20..638
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="pBR322 origin"
                     /Transferred_Similarity="98.87%"
                     /label="pBR322 origin"
                     /note="Geneious type: origin of replication"
     gene            complement(686..1501)
                     /gene="KanR2 (variant)"
                     /modified_by="User"
                     /label="KanR2"
     promoter        1829..1845
                     /modified_by="User"
                     /label="T7 promoter"
     misc_marker     1846
                     /created_by="User"
                     /label="G for T7 transcription"
     misc_RNA        1847..1866
                     /created_by="User"
                     /modified_by="User"
                     /label="AsCas12a crRNA"
     misc_marker     1867..1936
                     /created_by="User"
                     /modified_by="User"
                     /label="BsaI cassette"
     misc_feature    complement(1868..1873)
                     /Recognition_pattern="GGTCTC(1/5)"
                     /annotation_group="GGTCTC"
                     /label="BsaI"
                     /note="Geneious type: restriction site"
     misc_feature    1930..1935
                     /Recognition_pattern="GGTCTC(1/5)"
                     /annotation_group="GGTCTC"
                     /label="BsaI"
                     /note="Geneious type: restriction site"
ORIGIN      
        1 tgggcctttc ttcggtagaa aatcaaagga tcttcttgag atcctttttt tctgcgcgta
       61 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
      121 gagctaccaa ctctttttcc gaggtaactg gcttcagcag agcgcagata ccaaatactg
      181 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
      241 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
      301 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
      361 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
      421 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
      481 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
      541 tttatagtcc tgtcgggttt cgccacctct gacttgagca tcgatttttg tgatgctcgt
      601 caggggggcg gagcctatgg aaaaacgcca gcaacgcaga aaggcccacc cgaaggtgag
      661 ccaggtgatt acatttaggt cctcattaga aaaactcatc gagcatcaag tgaaactgca
      721 atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc tgtaatgaag
      781 gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg tctgcgattc
      841 cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaata aggttatcaa
      901 gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaagagt ttatgcattt
      961 ctttccagac ttgttcaaca ggccagccat tacgctcgtc atcaaaatca ctcgcaccaa
     1021 ccaaaccgtt attcattcgt gattgcgcct gagcgagacg aaatacgcga tcgccgttaa
     1081 aaggacaatt acaaacagga atcgaatgca accggcgcag gaacactgcc agcgcatcaa
     1141 caatattttc acctgaatca ggatattctt ctaatacctg gaatgctgtt ttccctggga
     1201 tcgcagtggt gagtaaccat gcatcatcag gagtacggat aaaatgcttg atggtcggaa
     1261 gaggcataaa ttccgtcagc cagtttagcc tgaccatctc atctgtaaca tcattggcaa
     1321 cgctaccttt gccatgtttc agaaacaact ctggcgcatc gggcttccca tacaatcgat
     1381 agattgtcgc acctgattgc ccgacattat cgcgagccca tttataccca tataaatcag
     1441 catccatgtt ggaatttaat cgcggcttca agcaagacgt ttcccgttga atatggctca
     1501 ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc
     1561 ttatttcatt atggtgaaag ttggaacctc ttacgtgccg atcaagtcaa aagcctccgg
     1621 tcggaggctt ttgactttct gctatggagg tcaggtatga tttaaatggt cagtattgag
     1681 cctcaggaaa cagctatgac atcaagctga ctagataatc tagctgatcg tggaccgatc
     1741 atacgtataa tgccgtaaga tcacgggtcg cagcacagct cgcggtccag tagtgatcga
     1801 cactgctcga tccgctcgca ccgctagcta atacgactca ctatagtaat ttctactctt
     1861 gtagatggag acccatgcca tagcgttgtt cggaatatga atttttgaac agattcacca
     1921 acacctagtg gtctccttta aaaagcttgg atcgacgaga gcagcgcgac tggatctgtc
     1981 gcccgtctca aacgcaaccc tccggcggtc gcatatcatt caggacgagc ctcagactcc
     2041 agcgtaactg gactgcaatc aactcactgg ctcaccttcc ggtccacgat cagctagaat
     2101 caagctgact agataaactg gccgtcgttt tacacggg
//
