LOCUS       Exported               10497 bp ds-DNA     circular SYN 29-AUG-2018
DEFINITION  pCAG-CFP.
ACCESSION   urn.local...h-98sgkio
VERSION     .
KEYWORDS    pCAG-hdAsCas12a(D908A)-NLS(nucleoplasmin)-gs-3xHA-gs-VPR (RTW876)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10497)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 29, 2018 from SnapGene 4.2.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10497
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        4..383
                     /label=CMV Enhancer
                     /note="/Color=#ffbb33"
                     /note="/created_by=User"
                     /note="/modified_by=User"
     promoter        385..660
                     /label=chicken beta-actin promoter
                     /note="/created_by=User"
                     /note="/modified_by=User"
     promoter        661..1677
                     /label=chimeric intron
                     /note="/Color=#eeeeee"
                     /note="/created_by=User"
                     /note="/modified_by=User"
     misc_feature    1770..1779
                     /label=Kozak
                     /label=nonstandard type: motif
                     /note="/created_by=User"
     CDS             1776..5696
                     /codon_start=1
                     /label=human codon optimized DNase inactive (D908A) As
                     /label=human codon optimized DNase inactive (D908A) 
                     AsCas12a
                     /note="/modified_by=User"
                     /translation="MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARND
                     HYKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRN
                     AIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFD
                     KFTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHF
                     ENVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVL
                     NLAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLL
                     RNENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTG
                     KITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTL
                     KKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKA
                     RNYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYK
                     ALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEP
                     LEITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSID
                     LSSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKG
                     HHGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLK
                     DQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFF
                     HVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIARGERNLIYITVIDSTGKILEQR
                     SLNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAV
                     VVLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQ
                     FTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYD
                     VKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIEN
                     HRFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQM
                     RNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKES
                     KDLKLQNGISNQDWLAYIQELRN"
     misc_feature    4497..4499
                     /label=D908A
                     /label=nonstandard type: misc_marker
                     /note="/created_by=User"
                     /note="/modified_by=User"
     misc_feature    5417
                     /label=silent mutation to remove NcoI site
                     /label=nonstandard type: misc_marker
                     /note="/created_by=User"
     misc_feature    5552
                     /label=silent mutation to remove NcoI site
                     /label=nonstandard type: misc_marker
                     /note="/created_by=User"
     CDS             5697..5744
                     /label=nucleoplasmin NLS
                     /note="/Color=#6bba70"
                     /note="/created_by=User"
                     /note="/modified_by=User"
     CDS             5745..5750
                     /label=GS-linker
                     /note="/Color=#ff7400"
                     /note="/created_by=User"
                     /note="/modified_by=User"
     CDS             5751..5831
                     /label=3x HA tag
                     /note="/Color=#ff0059"
                     /note="/created_by=User"
                     /note="/modified_by=User"
     CDS             5832..5837
                     /label=GS-linker
                     /note="/Color=#ff7400"
                     /note="/created_by=User"
     CDS             5838..7430
                     /label=VPR from Church Lab
                     /note="/Color=#008c00"
                     /note="/created_by=User"
                     /note="/modified_by=User"
     CDS             6036..6056
                     /label=SV40 NLS
                     /note="/Color=#6bba70"
                     /note="/created_by=User"
     polyA_site      7584..7639
                     /label=-beta-globin poly(A) signal
                     /note="/created_by=User"
                     /note="/modified_by=User"
     promoter        complement(8049..8078)
                     /label=lac_promoter
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN 
                     GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYK*"
     promoter        8184..8363
                     /label=SV40_promoter
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN 
                     GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYK*"
     rep_origin      8219..8296
                     /label=SV40_origin
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN 
                     GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYK*"
     polyA_signal    8374..8508
                     /label=SV40 poly(A) signal
                     /note="/created_by=User"
                     /note="/modified_by=User"
     rep_origin      complement(8732..9351)
                     /direction=LEFT
                     /label=pBR322_origin
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN 
                     GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYK*"
     gene            complement(9506..10366)
                     /gene="Ampicillin"
                     /label=Ampicillin
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN 
                     GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYK*"
     promoter        complement(10408..10436)
                     /label=AmpR_promoter
                     /note="/modified_by=User"
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggg ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg
      541 ggcggggcga ggcggagagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt
      601 ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg
      661 ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg
      721 ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctcct
      781 ccgggctgta attagcgctt ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa
      841 agccttgagg ggctccggga gggccctttg tgcgggggga gcggctcggg gggtgcgtgc
      901 gtgtgtgtgt gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg ctgtgagcgc
      961 tgcgggcgcg gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag cgcggccggg
     1021 ggcggtgccc cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg
     1081 tgcgtggggg ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc ccccctgcac
     1141 ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg tacggggcgt
     1201 ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg
     1261 gggccgcctc gggccgggga gggctcgggg gaggggcgcg gcggcccccg gagcgccggc
     1321 ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg
     1381 cagggacttc ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc
     1441 ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag
     1501 ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg gggctgtccg
     1561 cggggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg
     1621 accggcggct ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc
     1681 ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt ctgcagtcga
     1741 cggtaccgcg ggcccgggat ccaccggtcg ccaccatgac acagttcgag ggctttacca
     1801 acctgtatca ggtgagcaag acactgcggt ttgagctgat cccacagggc aagaccctga
     1861 agcacatcca ggagcagggc ttcatcgagg aggacaaggc ccgcaatgat cactacaagg
     1921 agctgaagcc catcatcgat cggatctaca agacctatgc cgaccagtgc ctgcagctgg
     1981 tgcagctgga ttgggagaac ctgagcgccg ccatcgactc ctatagaaag gagaaaaccg
     2041 aggagacaag gaacgccctg atcgaggagc aggccacata tcgcaatgcc atccacgact
     2101 acttcatcgg ccggacagac aacctgaccg atgccatcaa taagagacac gccgagatct
     2161 acaagggcct gttcaaggcc gagctgttta atggcaaggt gctgaagcag ctgggcaccg
     2221 tgaccacaac cgagcacgag aacgccctgc tgcggagctt cgacaagttt acaacctact
     2281 tctccggctt ttatgagaac aggaagaacg tgttcagcgc cgaggatatc agcacagcca
     2341 tcccacaccg catcgtgcag gacaacttcc ccaagtttaa ggagaattgt cacatcttca
     2401 cacgcctgat caccgccgtg cccagcctgc gggagcactt tgagaacgtg aagaaggcca
     2461 tcggcatctt cgtgagcacc tccatcgagg aggtgttttc cttccctttt tataaccagc
     2521 tgctgacaca gacccagatc gacctgtata accagctgct gggaggaatc tctcgggagg
     2581 caggcaccga gaagatcaag ggcctgaacg aggtgctgaa tctggccatc cagaagaatg
     2641 atgagacagc ccacatcatc gcctccctgc cacacagatt catccccctg tttaagcaga
     2701 tcctgtccga taggaacacc ctgtctttca tcctggagga gtttaagagc gacgaggaag
     2761 tgatccagtc cttctgcaag tacaagacac tgctgagaaa cgagaacgtg ctggagacag
     2821 ccgaggccct gtttaacgag ctgaacagca tcgacctgac acacatcttc atcagccaca
     2881 agaagctgga gacaatcagc agcgccctgt gcgaccactg ggatacactg aggaatgccc
     2941 tgtatgagcg gagaatctcc gagctgacag gcaagatcac caagtctgcc aaggagaagg
     3001 tgcagcgcag cctgaagcac gaggatatca acctgcagga gatcatctct gccgcaggca
     3061 aggagctgag cgaggccttc aagcagaaaa ccagcgagat cctgtcccac gcacacgccg
     3121 ccctggatca gccactgcct acaaccctga agaagcagga ggagaaggag atcctgaagt
     3181 ctcagctgga cagcctgctg ggcctgtacc acctgctgga ctggtttgcc gtggatgagt
     3241 ccaacgaggt ggaccccgag ttctctgccc ggctgaccgg catcaagctg gagatggagc
     3301 cttctctgag cttctacaac aaggccagaa attatgccac caagaagccc tactccgtgg
     3361 agaagttcaa gctgaacttt cagatgccta cactggcctc tggctgggac gtgaataagg
     3421 agaagaacaa tggcgccatc ctgtttgtga agaacggcct gtactatctg ggcatcatgc
     3481 caaagcagaa gggcaggtat aaggccctga gcttcgagcc cacagagaaa accagcgagg
     3541 gctttgataa gatgtactat gactacttcc ctgatgccgc caagatgatc ccaaagtgca
     3601 gcacccagct gaaggccgtg acagcccact ttcagaccca cacaaccccc atcctgctgt
     3661 ccaacaattt catcgagcct ctggagatca caaaggagat ctacgacctg aacaatcctg
     3721 agaaggagcc aaagaagttt cagacagcct acgccaagaa aaccggcgac cagaagggct
     3781 acagagaggc cctgtgcaag tggatcgact tcacaaggga ttttctgtcc aagtatacca
     3841 agacaacctc tatcgatctg tctagcctgc ggccatcctc tcagtataag gacctgggcg
     3901 agtactatgc cgagctgaat cccctgctgt accacatcag cttccagaga atcgccgaga
     3961 aggagatcat ggatgccgtg gagacaggca agctgtacct gttccagatc tataacaagg
     4021 actttgccaa gggccaccac ggcaagccta atctgcacac actgtattgg accggcctgt
     4081 tttctccaga gaacctggcc aagacaagca tcaagctgaa tggccaggcc gagctgttct
     4141 accgccctaa gtccaggatg aagaggatgg cacaccggct gggagagaag atgctgaaca
     4201 agaagctgaa ggatcagaaa accccaatcc ccgacaccct gtaccaggag ctgtacgact
     4261 atgtgaatca cagactgtcc cacgacctgt ctgatgaggc cagggccctg ctgcccaacg
     4321 tgatcaccaa ggaggtgtct cacgagatca tcaaggatag gcgctttacc agcgacaagt
     4381 tctttttcca cgtgcctatc acactgaact atcaggccgc caattcccca tctaagttca
     4441 accagagggt gaatgcctac ctgaaggagc accccgagac acctatcatc ggcatcgccc
     4501 ggggcgagag aaacctgatc tatatcacag tgatcgactc caccggcaag atcctggagc
     4561 agcggagcct gaacaccatc cagcagtttg attaccagaa gaagctggac aacagggaga
     4621 aggagagggt ggcagcaagg caggcctggt ctgtggtggg cacaatcaag gatctgaagc
     4681 agggctatct gagccaggtc atccacgaga tcgtggacct gatgatccac taccaggccg
     4741 tggtggtgct ggagaacctg aatttcggct ttaagagcaa gaggaccggc atcgccgaga
     4801 aggccgtgta ccagcagttc gagaagatgc tgatcgataa gctgaattgc ctggtgctga
     4861 aggactatcc agcagagaaa gtgggaggcg tgctgaaccc ataccagctg acagaccagt
     4921 tcacctcctt tgccaagatg ggcacccagt ctggcttcct gttttacgtg cctgccccat
     4981 atacatctaa gatcgatccc ctgaccggct tcgtggaccc cttcgtgtgg aaaaccatca
     5041 agaatcacga gagccgcaag cacttcctgg agggcttcga ctttctgcac tacgacgtga
     5101 aaaccggcga cttcatcctg cactttaaga tgaacagaaa tctgtccttc cagaggggcc
     5161 tgcccggctt tatgcctgca tgggatatcg tgttcgagaa gaacgagaca cagtttgacg
     5221 ccaagggcac ccctttcatc gccggcaaga gaatcgtgcc agtgatcgag aatcacagat
     5281 tcaccggcag ataccgggac ctgtatcctg ccaacgagct gatcgccctg ctggaggaga
     5341 agggcatcgt gttcagggat ggctccaaca tcctgccaaa gctgctggag aatgacgatt
     5401 ctcacgccat cgacacgatg gtggccctga tccgcagcgt gctgcagatg cggaactcca
     5461 atgccgccac aggcgaggac tatatcaaca gccccgtgcg cgatctgaat ggcgtgtgct
     5521 tcgactcccg gtttcagaac ccagagtggc caatggacgc cgatgccaat ggcgcctacc
     5581 acatcgccct gaagggccag ctgctgctga atcacctgaa ggagagcaag gatctgaagc
     5641 tgcagaacgg catctccaat caggactggc tggcctacat ccaggagctg cgcaacaaaa
     5701 ggccggcggc cacgaaaaag gccggccagg caaaaaagaa aaagggatcc tacccatacg
     5761 atgttccaga ttacgcttat ccctacgacg tgcctgatta tgcataccca tatgatgtcc
     5821 ccgactatgc cggaagcgag gccagcggtt ccggacgggc tgacgcattg gacgattttg
     5881 atctggatat gctgggaagt gacgccctcg atgattttga ccttgacatg cttggttcgg
     5941 atgcccttga tgactttgac ctcgacatgc tcggcagtga cgcccttgat gatttcgacc
     6001 tggacatgct gattaactct agaagttccg gatctccgaa aaagaaacgc aaagttggta
     6061 gccagtacct gcccgacacc gacgaccggc accggatcga ggaaaagcgg aagcggacct
     6121 acgagacatt caagagcatc atgaagaagt cccccttcag cggccccacc gaccctagac
     6181 ctccacctag aagaatcgcc gtgcccagca gatccagcgc cagcgtgcca aaacctgccc
     6241 cccagcctta ccccttcacc agcagcctga gcaccatcaa ctacgacgag ttccctacca
     6301 tggtgttccc cagcggccag atctctcagg cctctgctct ggctccagcc cctcctcagg
     6361 tgctgcctca ggctcctgct cctgcaccag ctccagccat ggtgtctgca ctggctcagg
     6421 caccagcacc cgtgcctgtg ctggctcctg gacctccaca ggctgtggct ccaccagccc
     6481 ctaaacctac acaggccggc gagggcacac tgtctgaagc tctgctgcag ctgcagttcg
     6541 acgacgagga tctgggagcc ctgctgggaa acagcaccga tcctgccgtg ttcaccgacc
     6601 tggccagcgt ggacaacagc gagttccagc agctgctgaa ccagggcatc cctgtggccc
     6661 ctcacaccac cgagcccatg ctgatggaat accccgaggc catcacccgg ctcgtgacag
     6721 gcgctcagag gcctcctgat ccagctcctg cccctctggg agcaccaggc ctgcctaatg
     6781 gactgctgtc tggcgacgag gacttcagct ctatcgccga tatggatttc tcagccttgc
     6841 tgggctctgg cagcggcagc cgggattcca gggaagggat gtttttgccg aagcctgagg
     6901 ccggctccgc tattagtgac gtgtttgagg gccgcgaggt gtgccagcca aaacgaatcc
     6961 ggccatttca tcctccagga agtccatggg ccaaccgccc actccccgcc agcctcgcac
     7021 caacaccaac cggtccagta catgagccag tcgggtcact gaccccggca ccagtccctc
     7081 agccactgga tccagcgccc gcagtgactc ccgaggccag tcacctgttg gaggatcccg
     7141 atgaagagac gagccaggct gtcaaagccc ttcgggagat ggccgatact gtgattcccc
     7201 agaaggaaga ggctgcaatc tgtggccaaa tggacctttc ccatccgccc ccaaggggcc
     7261 atctggatga gctgacaacc acacttgagt ccatgaccga ggatctgaac ctggactcac
     7321 ccctgacccc ggaattgaac gagattctgg ataccttcct gaacgacgag tgcctcttgc
     7381 atgccatgca tatcagcaca ggactgtcca tcttcgacac atctctgttt taagcggccg
     7441 cactcctcag gtgcaggctg cctatcagaa ggtggtggct ggtgtggcca atgccctggc
     7501 tcacaaatac cactgagatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc
     7561 ccttgagcat ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg
     7621 gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt taaaacatca
     7681 gaatgagtat ttggtttaga gtttggcaac atatgcccat atgctggctg ccatgaacaa
     7741 aggttggcta taaagaggtc atcagtatat gaaacagccc cctgctgtcc attccttatt
     7801 ccatagaaaa gccttgactt gaggttagat tttttttata ttttgttttg tgttattttt
     7861 ttctttaaca tccctaaaat tttccttaca tgttttacta gccagatttt tcctcctctc
     7921 ctgactactc ccagtcatag ctgtccctct tctcttatgg agatccctcg acctgcagcc
     7981 caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     8041 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     8101 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     8161 gccagcggat ccgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     8221 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     8281 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     8341 cttttttgga ggcctaggct tttgcaaaaa gctaacttgt ttattgcagc ttataatggt
     8401 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     8461 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatccg ctgcattaat
     8521 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     8581 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     8641 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     8701 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     8761 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     8821 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     8881 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     8941 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     9001 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     9061 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     9121 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     9181 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     9241 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     9301 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     9361 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     9421 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     9481 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     9541 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     9601 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     9661 cggctccaga tttatcagca ataaaccagc cagccggaag ggccaagcgc agaagtggtc
     9721 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     9781 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     9841 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     9901 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     9961 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
    10021 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
    10081 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
    10141 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
    10201 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
    10261 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
    10321 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
    10381 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
    10441 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctg
//
