LOCUS pCAG-rAPOBEC1-gs 9904 bp DNA circular SYN 06-JUL-2018 DEFINITION pCAG-CFP. ACCESSION urn.local...s-98sgkip KEYWORDS . SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers promoter 4..383 /created_by="User" /Color="#ffbb33" /modified_by="User" /label="CMV Enhancer" promoter 386..661 /created_by="User" /modified_by="User" /label="chicken beta-actin promoter" promoter 662..1670 /created_by="User" /Color="#eeeeee" /modified_by="User" /label="chimeric intron" CDS 1769..2458 /created_by="User" /Color="#ff7777" /modified_by="User" /label="rAPOBEC1" CDS 1772..1774 /created_by="User" /label="Inserted Gly for NcoI site" CDS 2459..2482 /created_by="User" /Color="#ff7400" /label="Gly-Ser linker" CDS 2483..2530 /created_by="User" /Color="#ff9911" /modified_by="User" /label="XTEN linker" CDS 2531..2554 /created_by="User" /Color="#ff7400" /label="Gly-Ser linker" CDS 2555..6472 /translation="MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARN DHYKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATY RNAIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLR SFDKFTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSL REHFENVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKG LNEVLNLAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFC KYKTLLRNENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYER RISELTGKITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAAL DQPLPTTLKKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEME PSLSFYNKARNYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLG IMPKQKGRYKALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTT PILLSNNFIEPLEITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRD FLSKYTKTTSIDLSSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKL YLFQIYNKDFAKGHHGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRM AHRLGEKMLNKKLKDQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSH EIIKDRRFTSDKFFFHVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNL IYITVIDSTGKILEQRSLNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYL SQVIHEIVDLMIHYQAVVVLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKD YPAEKVGGVLNPYQLTDQFTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTI KNHESRKHFLEGFDFLHYDVKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQ FDAKGTPFIAGKRIVPVIENHRFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLL ENDDSHAIDTMVALIRSVLQMRNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDA DANGAYHIALKGQLLLNHLKESKDLKLQNGISNQDWLAYIQELRNKRPAATKKAGQAK KKKGSYPYDVPDYAYPYDVPDYAYPYDVPDYA*" /modified_by="User" /label="human codon optimized DNase inactive (D908A) enAsCas12a(E174R/S542R/K548R)" misc_marker 3071..3073 /created_by="User" /label="E174R" misc_marker 4175..4177 /created_by="User" /label="S542R" misc_marker 4193..4195 /created_by="User" /label="K548R" misc_difference 5273..5275 /created_by="User" /label="D908A" misc_marker 6192..6197 /created_by="User" /modified_by="User" /label="silent mutation to remove NcoI site" misc_marker 6327..6332 /created_by="User" /modified_by="User" /label="silent mutation to remove NcoI site" CDS 6473..6520 /created_by="User" /Color="#6bba70" /modified_by="User" /label="nucleoplasmin NLS" CDS 6521..6556 /created_by="User" /Color="#ff7400" /modified_by="User" /label="Gly-Ser linker" CDS 6557..6805 /created_by="User" /Color="#9999ff" /modified_by="User" /label="UGI" CDS 6806..6817 /created_by="User" /Color="#ff7400" /modified_by="User" /label="Gly-Ser linker" CDS 6818..6838 /codon_start=1 /product="nuclear localization signal of SV40 large T antigen" /translation="PKKKRKV" /Color="#6bba70" /modified_by="User" /label="SV40 NLS" terminator 6993..7048 /created_by="User" /modified_by="User" /label="-beta-globin poly(A) signal" promoter complement(7456..7485) /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MVLLEFVTAAGITLGMDELYK*" /label="lac_promoter" promoter 7591..7770 /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MVLLEFVTAAGITLGMDELYK*" /label="SV40_promoter" rep_origin 7626..7703 /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MVLLEFVTAAGITLGMDELYK*" /label="SV40_origin" terminator 7784..7915 /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MVLLEFVTAAGITLGMDELYK*" /label="SV40_PA_terminator" rep_origin complement(8139..8758) /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MVLLEFVTAAGITLGMDELYK*" /label="pBR322_origin" gene complement(8913..9773) /gene="Ampicillin" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MVLLEFVTAAGITLGMDELYK*" /label="Ampicillin" promoter complement(9815..9843) /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" /label="AmpR_promoter" ORIGIN 1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata 61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc 121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag 181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac 241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg 301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg 361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc 421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca 481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg 541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt 601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc 661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc 721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc 781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag 841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg 901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg 961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc 1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg 1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc 1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc 1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg 1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct 1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg 1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc 1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt 1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg 1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg 1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca 1681 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attctgcagt cgacggtacc 1741 gcgggcccgg gatccaccgg tcgccaccat gggcagctca gagactggcc cagtggctgt 1801 ggaccccaca ttgagacggc ggatcgagcc ccatgagttt gaggtattct tcgatccgag 1861 agagctccgc aaggagacct gcctgcttta cgaaattaat tgggggggcc ggcactccat 1921 ttggcgacat acatcacaga acactaacaa gcacgtcgaa gtcaacttca tcgagaagtt 1981 cacgacagaa agatatttct gtccgaacac aaggtgcagc attacctggt ttctcagctg 2041 gagcccatgc ggcgaatgta gtagggccat cactgaattc ctgtcaaggt atccccacgt 2101 cactctgttt atttacatcg caaggctgta ccaccacgct gacccccgca atcgacaagg 2161 cctgcgggat ttgatctctt caggtgtgac tatccaaatt atgactgagc aggagtcagg 2221 atactgctgg agaaactttg tgaattatag cccgagtaat gaagcccact ggcctaggta 2281 tccccatctg tgggtacgac tgtacgttct tgaactgtac tgcatcatac tgggcctgcc 2341 tccttgtctc aacattctga gaaggaagca gccacagctg acattcttta ccatcgctct 2401 tcagtcttgt cattaccagc gactgccccc acacattctc tgggccaccg ggttgaaatc 2461 tggtggttct tctggtggtt ctagcggcag cgagactccc gggacctcag agtccgccac 2521 acccgaaagt tccggaggga gtagcggcgg gtctacacag ttcgagggct ttaccaacct 2581 gtatcaggtg agcaagacac tgcggtttga gctgatccca cagggcaaga ccctgaagca 2641 catccaggag cagggcttca tcgaggagga caaggcccgc aatgatcact acaaggagct 2701 gaagcccatc atcgatcgga tctacaagac ctatgccgac cagtgcctgc agctggtgca 2761 gctggattgg gagaacctga gcgccgccat cgactcctat agaaaggaga aaaccgagga 2821 gacaaggaac gccctgatcg aggagcaggc cacatatcgc aatgccatcc acgactactt 2881 catcggccgg acagacaacc tgaccgatgc catcaataag agacacgccg agatctacaa 2941 gggcctgttc aaggccgagc tgtttaatgg caaggtgctg aagcagctgg gcaccgtgac 3001 cacaaccgag cacgagaacg ccctgctgcg gagcttcgac aagtttacaa cctacttctc 3061 cggcttttat agaaacagga agaacgtgtt cagcgccgag gatatcagca cagccatccc 3121 acaccgcatc gtgcaggaca acttccccaa gtttaaggag aattgtcaca tcttcacacg 3181 cctgatcacc gccgtgccca gcctgcggga gcactttgag aacgtgaaga aggccatcgg 3241 catcttcgtg agcacctcca tcgaggaggt gttttccttc cctttttata accagctgct 3301 gacacagacc cagatcgacc tgtataacca gctgctggga ggaatctctc gggaggcagg 3361 caccgagaag atcaagggcc tgaacgaggt gctgaatctg gccatccaga agaatgatga 3421 gacagcccac atcatcgcct ccctgccaca cagattcatc cccctgttta agcagatcct 3481 gtccgatagg aacaccctgt ctttcatcct ggaggagttt aagagcgacg aggaagtgat 3541 ccagtccttc tgcaagtaca agacactgct gagaaacgag aacgtgctgg agacagccga 3601 ggccctgttt aacgagctga acagcatcga cctgacacac atcttcatca gccacaagaa 3661 gctggagaca atcagcagcg ccctgtgcga ccactgggat acactgagga atgccctgta 3721 tgagcggaga atctccgagc tgacaggcaa gatcaccaag tctgccaagg agaaggtgca 3781 gcgcagcctg aagcacgagg atatcaacct gcaggagatc atctctgccg caggcaagga 3841 gctgagcgag gccttcaagc agaaaaccag cgagatcctg tcccacgcac acgccgccct 3901 ggatcagcca ctgcctacaa ccctgaagaa gcaggaggag aaggagatcc tgaagtctca 3961 gctggacagc ctgctgggcc tgtaccacct gctggactgg tttgccgtgg atgagtccaa 4021 cgaggtggac cccgagttct ctgcccggct gaccggcatc aagctggaga tggagccttc 4081 tctgagcttc tacaacaagg ccagaaatta tgccaccaag aagccctact ccgtggagaa 4141 gttcaagctg aactttcaga tgcctacact ggccagaggc tgggacgtga atagagagaa 4201 gaacaatggc gccatcctgt ttgtgaagaa cggcctgtac tatctgggca tcatgccaaa 4261 gcagaagggc aggtataagg ccctgagctt cgagcccaca gagaaaacca gcgagggctt 4321 tgataagatg tactatgact acttccctga tgccgccaag atgatcccaa agtgcagcac 4381 ccagctgaag gccgtgacag cccactttca gacccacaca acccccatcc tgctgtccaa 4441 caatttcatc gagcctctgg agatcacaaa ggagatctac gacctgaaca atcctgagaa 4501 ggagccaaag aagtttcaga cagcctacgc caagaaaacc ggcgaccaga agggctacag 4561 agaggccctg tgcaagtgga tcgacttcac aagggatttt ctgtccaagt ataccaagac 4621 aacctctatc gatctgtcta gcctgcggcc atcctctcag tataaggacc tgggcgagta 4681 ctatgccgag ctgaatcccc tgctgtacca catcagcttc cagagaatcg ccgagaagga 4741 gatcatggat gccgtggaga caggcaagct gtacctgttc cagatctata acaaggactt 4801 tgccaagggc caccacggca agcctaatct gcacacactg tattggaccg gcctgttttc 4861 tccagagaac ctggccaaga caagcatcaa gctgaatggc caggccgagc tgttctaccg 4921 ccctaagtcc aggatgaaga ggatggcaca ccggctggga gagaagatgc tgaacaagaa 4981 gctgaaggat cagaaaaccc caatccccga caccctgtac caggagctgt acgactatgt 5041 gaatcacaga ctgtcccacg acctgtctga tgaggccagg gccctgctgc ccaacgtgat 5101 caccaaggag gtgtctcacg agatcatcaa ggataggcgc tttaccagcg acaagttctt 5161 tttccacgtg cctatcacac tgaactatca ggccgccaat tccccatcta agttcaacca 5221 gagggtgaat gcctacctga aggagcaccc cgagacacct atcatcggca tcgcccgggg 5281 cgagagaaac ctgatctata tcacagtgat cgactccacc ggcaagatcc tggagcagcg 5341 gagcctgaac accatccagc agtttgatta ccagaagaag ctggacaaca gggagaagga 5401 gagggtggca gcaaggcagg cctggtctgt ggtgggcaca atcaaggatc tgaagcaggg 5461 ctatctgagc caggtcatcc acgagatcgt ggacctgatg atccactacc aggccgtggt 5521 ggtgctggag aacctgaatt tcggctttaa gagcaagagg accggcatcg ccgagaaggc 5581 cgtgtaccag cagttcgaga agatgctgat cgataagctg aattgcctgg tgctgaagga 5641 ctatccagca gagaaagtgg gaggcgtgct gaacccatac cagctgacag accagttcac 5701 ctcctttgcc aagatgggca cccagtctgg cttcctgttt tacgtgcctg ccccatatac 5761 atctaagatc gatcccctga ccggcttcgt ggaccccttc gtgtggaaaa ccatcaagaa 5821 tcacgagagc cgcaagcact tcctggaggg cttcgacttt ctgcactacg acgtgaaaac 5881 cggcgacttc atcctgcact ttaagatgaa cagaaatctg tccttccaga ggggcctgcc 5941 cggctttatg cctgcatggg atatcgtgtt cgagaagaac gagacacagt ttgacgccaa 6001 gggcacccct ttcatcgccg gcaagagaat cgtgccagtg atcgagaatc acagattcac 6061 cggcagatac cgggacctgt atcctgccaa cgagctgatc gccctgctgg aggagaaggg 6121 catcgtgttc agggatggct ccaacatcct gccaaagctg ctggagaatg acgattctca 6181 cgccatcgac acgatggtgg ccctgatccg cagcgtgctg cagatgcgga actccaatgc 6241 cgccacaggc gaggactata tcaacagccc cgtgcgcgat ctgaatggcg tgtgcttcga 6301 ctcccggttt cagaacccag agtggccaat ggacgccgat gccaatggcg cctaccacat 6361 cgccctgaag ggccagctgc tgctgaatca cctgaaggag agcaaggatc tgaagctgca 6421 gaacggcatc tccaatcagg actggctggc ctacatccag gagctgcgca acaaaaggcc 6481 ggcggccacg aaaaaggccg gccaggcaaa aaagaaaaag ggatcctctg gtggttctgg 6541 aggatctggt ggttctacta atctgtcaga tattattgaa aaggagaccg gtaagcaact 6601 ggttatccag gaatccatcc tcatgctccc agaggaggtg gaagaagtca ttgggaacaa 6661 gccggaaagc gatatactcg tgcacaccgc ctacgacgag agcaccgacg agaatgtcat 6721 gcttctgact agcgacgccc ctgaatacaa gccttgggct ctggtcatac aggatagcaa 6781 cggtgagaac aagattaaga tgctctctgg tggttctccc aagaagaaga ggaaagtcta 6841 aagcggccgc actcctcagg tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa 6901 tgccctggct cacaaatacc actgagatct ttttccctct gccaaaaatt atggggacat 6961 catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat 7021 agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt 7081 aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgccatat gctggctgcc 7141 atgaacaaag gtggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt 7201 ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt 7261 tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc 7321 tcctctcctg actactccca gtcatagctg tccctcttct cttatgaaga tccctcgacc 7381 tgcagcccaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg 7441 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa 7501 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac 7561 ctgtcgtgcc agcggatccg catctcaatt agtcagcaac catagtcccg cccctaactc 7621 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa 7681 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt 7741 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct aacttgttta ttgcagctta 7801 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 7861 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccgctg 7921 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct 7981 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 8041 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 8101 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 8161 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 8221 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 8281 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 8341 ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 8401 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 8461 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 8521 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 8581 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 8641 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 8701 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 8761 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 8821 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 8881 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 8941 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 9001 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 9061 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 9121 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 9181 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg 9241 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 9301 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 9361 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 9421 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 9481 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat 9541 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 9601 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 9661 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 9721 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 9781 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 9841 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 9901 cctg //