LOCUS       pCAG-rAPOBEC1-gs        9904 bp    DNA     circular SYN 06-JUL-2018
DEFINITION  pCAG-CFP.
ACCESSION   urn.local...s-98sgkip
KEYWORDS    .
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     promoter        4..383
                     /created_by="User"
                     /Color="#ffbb33"
                     /modified_by="User"
                     /label="CMV Enhancer"
     promoter        386..661
                     /created_by="User"
                     /modified_by="User"
                     /label="chicken beta-actin promoter"
     promoter        662..1670
                     /created_by="User"
                     /Color="#eeeeee"
                     /modified_by="User"
                     /label="chimeric intron"
     CDS             1769..2458
                     /created_by="User"
                     /Color="#ff7777"
                     /modified_by="User"
                     /label="rAPOBEC1"
     CDS             1772..1774
                     /created_by="User"
                     /label="Inserted Gly for NcoI site"
     CDS             2459..2482
                     /created_by="User"
                     /Color="#ff7400"
                     /label="Gly-Ser linker"
     CDS             2483..2530
                     /created_by="User"
                     /Color="#ff9911"
                     /modified_by="User"
                     /label="XTEN linker"
     CDS             2531..2554
                     /created_by="User"
                     /Color="#ff7400"
                     /label="Gly-Ser linker"
     CDS             2555..6472
                     /translation="MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARN
                     DHYKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATY
                     RNAIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLR
                     SFDKFTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSL
                     REHFENVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKG
                     LNEVLNLAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFC
                     KYKTLLRNENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYER
                     RISELTGKITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAAL
                     DQPLPTTLKKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEME
                     PSLSFYNKARNYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLG
                     IMPKQKGRYKALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTT
                     PILLSNNFIEPLEITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRD
                     FLSKYTKTTSIDLSSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKL
                     YLFQIYNKDFAKGHHGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRM
                     AHRLGEKMLNKKLKDQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSH
                     EIIKDRRFTSDKFFFHVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNL
                     IYITVIDSTGKILEQRSLNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYL
                     SQVIHEIVDLMIHYQAVVVLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKD
                     YPAEKVGGVLNPYQLTDQFTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTI
                     KNHESRKHFLEGFDFLHYDVKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQ
                     FDAKGTPFIAGKRIVPVIENHRFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLL
                     ENDDSHAIDTMVALIRSVLQMRNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDA
                     DANGAYHIALKGQLLLNHLKESKDLKLQNGISNQDWLAYIQELRNKRPAATKKAGQAK
                     KKKGSYPYDVPDYAYPYDVPDYAYPYDVPDYA*"
                     /modified_by="User"
                     /label="human codon optimized DNase inactive (D908A)
                     enAsCas12a(E174R/S542R/K548R)"
     misc_marker     3071..3073
                     /created_by="User"
                     /label="E174R"
     misc_marker     4175..4177
                     /created_by="User"
                     /label="S542R"
     misc_marker     4193..4195
                     /created_by="User"
                     /label="K548R"
     misc_difference 5273..5275
                     /created_by="User"
                     /label="D908A"
     misc_marker     6192..6197
                     /created_by="User"
                     /modified_by="User"
                     /label="silent mutation to remove NcoI site"
     misc_marker     6327..6332
                     /created_by="User"
                     /modified_by="User"
                     /label="silent mutation to remove NcoI site"
     CDS             6473..6520
                     /created_by="User"
                     /Color="#6bba70"
                     /modified_by="User"
                     /label="nucleoplasmin NLS"
     CDS             6521..6556
                     /created_by="User"
                     /Color="#ff7400"
                     /modified_by="User"
                     /label="Gly-Ser linker"
     CDS             6557..6805
                     /created_by="User"
                     /Color="#9999ff"
                     /modified_by="User"
                     /label="UGI"
     CDS             6806..6817
                     /created_by="User"
                     /Color="#ff7400"
                     /modified_by="User"
                     /label="Gly-Ser linker"
     CDS             6818..6838
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /translation="PKKKRKV"
                     /Color="#6bba70"
                     /modified_by="User"
                     /label="SV40 NLS"
     terminator      6993..7048
                     /created_by="User"
                     /modified_by="User"
                     /label="-beta-globin poly(A) signal"
     promoter        complement(7456..7485)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN
                     GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
                     /label="lac_promoter"
     promoter        7591..7770
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN
                     GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
                     /label="SV40_promoter"
     rep_origin      7626..7703
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN
                     GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
                     /label="SV40_origin"
     terminator      7784..7915
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN
                     GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
                     /label="SV40_PA_terminator"
     rep_origin      complement(8139..8758)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN
                     GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
                     /label="pBR322_origin"
     gene            complement(8913..9773)
                     /gene="Ampicillin"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN
                     GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
                     /label="Ampicillin"
     promoter        complement(9815..9843)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /label="AmpR_promoter"
ORIGIN      
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
     1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
     1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc
     1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg
     1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     1681 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attctgcagt cgacggtacc
     1741 gcgggcccgg gatccaccgg tcgccaccat gggcagctca gagactggcc cagtggctgt
     1801 ggaccccaca ttgagacggc ggatcgagcc ccatgagttt gaggtattct tcgatccgag
     1861 agagctccgc aaggagacct gcctgcttta cgaaattaat tgggggggcc ggcactccat
     1921 ttggcgacat acatcacaga acactaacaa gcacgtcgaa gtcaacttca tcgagaagtt
     1981 cacgacagaa agatatttct gtccgaacac aaggtgcagc attacctggt ttctcagctg
     2041 gagcccatgc ggcgaatgta gtagggccat cactgaattc ctgtcaaggt atccccacgt
     2101 cactctgttt atttacatcg caaggctgta ccaccacgct gacccccgca atcgacaagg
     2161 cctgcgggat ttgatctctt caggtgtgac tatccaaatt atgactgagc aggagtcagg
     2221 atactgctgg agaaactttg tgaattatag cccgagtaat gaagcccact ggcctaggta
     2281 tccccatctg tgggtacgac tgtacgttct tgaactgtac tgcatcatac tgggcctgcc
     2341 tccttgtctc aacattctga gaaggaagca gccacagctg acattcttta ccatcgctct
     2401 tcagtcttgt cattaccagc gactgccccc acacattctc tgggccaccg ggttgaaatc
     2461 tggtggttct tctggtggtt ctagcggcag cgagactccc gggacctcag agtccgccac
     2521 acccgaaagt tccggaggga gtagcggcgg gtctacacag ttcgagggct ttaccaacct
     2581 gtatcaggtg agcaagacac tgcggtttga gctgatccca cagggcaaga ccctgaagca
     2641 catccaggag cagggcttca tcgaggagga caaggcccgc aatgatcact acaaggagct
     2701 gaagcccatc atcgatcgga tctacaagac ctatgccgac cagtgcctgc agctggtgca
     2761 gctggattgg gagaacctga gcgccgccat cgactcctat agaaaggaga aaaccgagga
     2821 gacaaggaac gccctgatcg aggagcaggc cacatatcgc aatgccatcc acgactactt
     2881 catcggccgg acagacaacc tgaccgatgc catcaataag agacacgccg agatctacaa
     2941 gggcctgttc aaggccgagc tgtttaatgg caaggtgctg aagcagctgg gcaccgtgac
     3001 cacaaccgag cacgagaacg ccctgctgcg gagcttcgac aagtttacaa cctacttctc
     3061 cggcttttat agaaacagga agaacgtgtt cagcgccgag gatatcagca cagccatccc
     3121 acaccgcatc gtgcaggaca acttccccaa gtttaaggag aattgtcaca tcttcacacg
     3181 cctgatcacc gccgtgccca gcctgcggga gcactttgag aacgtgaaga aggccatcgg
     3241 catcttcgtg agcacctcca tcgaggaggt gttttccttc cctttttata accagctgct
     3301 gacacagacc cagatcgacc tgtataacca gctgctggga ggaatctctc gggaggcagg
     3361 caccgagaag atcaagggcc tgaacgaggt gctgaatctg gccatccaga agaatgatga
     3421 gacagcccac atcatcgcct ccctgccaca cagattcatc cccctgttta agcagatcct
     3481 gtccgatagg aacaccctgt ctttcatcct ggaggagttt aagagcgacg aggaagtgat
     3541 ccagtccttc tgcaagtaca agacactgct gagaaacgag aacgtgctgg agacagccga
     3601 ggccctgttt aacgagctga acagcatcga cctgacacac atcttcatca gccacaagaa
     3661 gctggagaca atcagcagcg ccctgtgcga ccactgggat acactgagga atgccctgta
     3721 tgagcggaga atctccgagc tgacaggcaa gatcaccaag tctgccaagg agaaggtgca
     3781 gcgcagcctg aagcacgagg atatcaacct gcaggagatc atctctgccg caggcaagga
     3841 gctgagcgag gccttcaagc agaaaaccag cgagatcctg tcccacgcac acgccgccct
     3901 ggatcagcca ctgcctacaa ccctgaagaa gcaggaggag aaggagatcc tgaagtctca
     3961 gctggacagc ctgctgggcc tgtaccacct gctggactgg tttgccgtgg atgagtccaa
     4021 cgaggtggac cccgagttct ctgcccggct gaccggcatc aagctggaga tggagccttc
     4081 tctgagcttc tacaacaagg ccagaaatta tgccaccaag aagccctact ccgtggagaa
     4141 gttcaagctg aactttcaga tgcctacact ggccagaggc tgggacgtga atagagagaa
     4201 gaacaatggc gccatcctgt ttgtgaagaa cggcctgtac tatctgggca tcatgccaaa
     4261 gcagaagggc aggtataagg ccctgagctt cgagcccaca gagaaaacca gcgagggctt
     4321 tgataagatg tactatgact acttccctga tgccgccaag atgatcccaa agtgcagcac
     4381 ccagctgaag gccgtgacag cccactttca gacccacaca acccccatcc tgctgtccaa
     4441 caatttcatc gagcctctgg agatcacaaa ggagatctac gacctgaaca atcctgagaa
     4501 ggagccaaag aagtttcaga cagcctacgc caagaaaacc ggcgaccaga agggctacag
     4561 agaggccctg tgcaagtgga tcgacttcac aagggatttt ctgtccaagt ataccaagac
     4621 aacctctatc gatctgtcta gcctgcggcc atcctctcag tataaggacc tgggcgagta
     4681 ctatgccgag ctgaatcccc tgctgtacca catcagcttc cagagaatcg ccgagaagga
     4741 gatcatggat gccgtggaga caggcaagct gtacctgttc cagatctata acaaggactt
     4801 tgccaagggc caccacggca agcctaatct gcacacactg tattggaccg gcctgttttc
     4861 tccagagaac ctggccaaga caagcatcaa gctgaatggc caggccgagc tgttctaccg
     4921 ccctaagtcc aggatgaaga ggatggcaca ccggctggga gagaagatgc tgaacaagaa
     4981 gctgaaggat cagaaaaccc caatccccga caccctgtac caggagctgt acgactatgt
     5041 gaatcacaga ctgtcccacg acctgtctga tgaggccagg gccctgctgc ccaacgtgat
     5101 caccaaggag gtgtctcacg agatcatcaa ggataggcgc tttaccagcg acaagttctt
     5161 tttccacgtg cctatcacac tgaactatca ggccgccaat tccccatcta agttcaacca
     5221 gagggtgaat gcctacctga aggagcaccc cgagacacct atcatcggca tcgcccgggg
     5281 cgagagaaac ctgatctata tcacagtgat cgactccacc ggcaagatcc tggagcagcg
     5341 gagcctgaac accatccagc agtttgatta ccagaagaag ctggacaaca gggagaagga
     5401 gagggtggca gcaaggcagg cctggtctgt ggtgggcaca atcaaggatc tgaagcaggg
     5461 ctatctgagc caggtcatcc acgagatcgt ggacctgatg atccactacc aggccgtggt
     5521 ggtgctggag aacctgaatt tcggctttaa gagcaagagg accggcatcg ccgagaaggc
     5581 cgtgtaccag cagttcgaga agatgctgat cgataagctg aattgcctgg tgctgaagga
     5641 ctatccagca gagaaagtgg gaggcgtgct gaacccatac cagctgacag accagttcac
     5701 ctcctttgcc aagatgggca cccagtctgg cttcctgttt tacgtgcctg ccccatatac
     5761 atctaagatc gatcccctga ccggcttcgt ggaccccttc gtgtggaaaa ccatcaagaa
     5821 tcacgagagc cgcaagcact tcctggaggg cttcgacttt ctgcactacg acgtgaaaac
     5881 cggcgacttc atcctgcact ttaagatgaa cagaaatctg tccttccaga ggggcctgcc
     5941 cggctttatg cctgcatggg atatcgtgtt cgagaagaac gagacacagt ttgacgccaa
     6001 gggcacccct ttcatcgccg gcaagagaat cgtgccagtg atcgagaatc acagattcac
     6061 cggcagatac cgggacctgt atcctgccaa cgagctgatc gccctgctgg aggagaaggg
     6121 catcgtgttc agggatggct ccaacatcct gccaaagctg ctggagaatg acgattctca
     6181 cgccatcgac acgatggtgg ccctgatccg cagcgtgctg cagatgcgga actccaatgc
     6241 cgccacaggc gaggactata tcaacagccc cgtgcgcgat ctgaatggcg tgtgcttcga
     6301 ctcccggttt cagaacccag agtggccaat ggacgccgat gccaatggcg cctaccacat
     6361 cgccctgaag ggccagctgc tgctgaatca cctgaaggag agcaaggatc tgaagctgca
     6421 gaacggcatc tccaatcagg actggctggc ctacatccag gagctgcgca acaaaaggcc
     6481 ggcggccacg aaaaaggccg gccaggcaaa aaagaaaaag ggatcctctg gtggttctgg
     6541 aggatctggt ggttctacta atctgtcaga tattattgaa aaggagaccg gtaagcaact
     6601 ggttatccag gaatccatcc tcatgctccc agaggaggtg gaagaagtca ttgggaacaa
     6661 gccggaaagc gatatactcg tgcacaccgc ctacgacgag agcaccgacg agaatgtcat
     6721 gcttctgact agcgacgccc ctgaatacaa gccttgggct ctggtcatac aggatagcaa
     6781 cggtgagaac aagattaaga tgctctctgg tggttctccc aagaagaaga ggaaagtcta
     6841 aagcggccgc actcctcagg tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa
     6901 tgccctggct cacaaatacc actgagatct ttttccctct gccaaaaatt atggggacat
     6961 catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat
     7021 agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt
     7081 aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgccatat gctggctgcc
     7141 atgaacaaag gtggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt
     7201 ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt
     7261 tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc
     7321 tcctctcctg actactccca gtcatagctg tccctcttct cttatgaaga tccctcgacc
     7381 tgcagcccaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     7441 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     7501 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     7561 ctgtcgtgcc agcggatccg catctcaatt agtcagcaac catagtcccg cccctaactc
     7621 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     7681 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     7741 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct aacttgttta ttgcagctta
     7801 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     7861 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccgctg
     7921 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     7981 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     8041 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     8101 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     8161 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     8221 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     8281 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     8341 ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     8401 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     8461 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     8521 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     8581 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     8641 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     8701 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     8761 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     8821 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     8881 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     8941 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     9001 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     9061 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     9121 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     9181 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     9241 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     9301 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     9361 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     9421 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     9481 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     9541 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     9601 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     9661 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     9721 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     9781 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     9841 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     9901 cctg
//
