LOCUS Exported 9973 bp ds-DNA circular SYN 29-AUG-2018 DEFINITION pCAG-CFP. ACCESSION urn.local...o-98sgkio VERSION . KEYWORDS pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/... SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9973) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 29, 2018 from SnapGene 4.2.4 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9973 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 4..383 /label=CMV Enhancer /note="/Color=#ffbb33" /note="/created_by=User" /note="/modified_by=User" promoter 385..660 /label=chicken beta-actin promoter /note="/created_by=User" /note="/modified_by=User" promoter 661..1677 /label=chimeric intron /note="/Color=#eeeeee" /note="/created_by=User" /note="/modified_by=User" CDS 1776..1781 /label=Start codon and inserted Gly for NcoI site /note="/created_by=User" /note="/modified_by=User" CDS 1782..1802 /codon_start=1 /product="nuclear localization signal of SV40 large T antigen" /label=SV40 NLS /note="/Color=#6bba70" /translation="PKKKRKV" CDS 1803..1811 /label=Gly-Ser linker /note="/Color=#ff7400" /note="/created_by=User" /note="/modified_by=User" CDS 1812..1832 /codon_start=1 /product="nuclear localization signal of SV40 large T antigen" /label=SV40 NLS /note="/Color=#6bba70" /translation="PKKKRKV" CDS 1833..1841 /label=Gly-Ser linker /note="/Color=#ff7400" /note="/created_by=User" /note="/modified_by=User" CDS 1842..2525 /label=rAPOBEC1 /note="/Color=#ff7777" /note="/created_by=User" /note="/modified_by=User" CDS 2526..2549 /label=Gly-Ser linker /note="/Color=#ff7400" /note="/created_by=User" CDS 2550..2597 /label=XTEN linker /note="/Color=#ff9911" /note="/created_by=User" /note="/modified_by=User" CDS 2598..2621 /label=Gly-Ser linker /note="/Color=#ff7400" /note="/created_by=User" CDS 2622..6539 /codon_start=1 /label=human codon optimized DNase inactive (D908A) en /label=human codon optimized DNase inactive (D908A) enAsCas12a(E174R/S542R/K548R) /note="/modified_by=User" /translation="MQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARNDH YKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRNA IHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFDK FTTYFSGFYRNRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHFE NVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVLN LAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLLR NENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTGK ITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTLK KQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKAR NYATKKPYSVEKFKLNFQMPTLARGWDVNREKNNGAILFVKNGLYYLGIMPKQKGRYKA LSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEPL EITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSIDL SSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKGH HGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLKD QKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFFH VPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIARGERNLIYITVIDSTGKILEQRS LNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAVV VLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQF TSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYDV KTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIENH RFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQMR NSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKESK DLKLQNGISNQDWLAYIQELRN" misc_feature 3138..3140 /label=E174R /label=nonstandard type: misc_marker /note="/created_by=User" misc_feature 4242..4244 /label=S542R /label=nonstandard type: misc_marker /note="/created_by=User" misc_feature 4260..4262 /label=K548R /label=nonstandard type: misc_marker /note="/created_by=User" misc_difference 5340..5342 /label=D908A /note="/created_by=User" misc_feature 6259..6264 /label=silent mutation to remove NcoI site /label=nonstandard type: misc_marker /note="/created_by=User" /note="/modified_by=User" misc_feature 6394..6399 /label=silent mutation to remove NcoI site /label=nonstandard type: misc_marker /note="/created_by=User" /note="/modified_by=User" CDS 6540..6587 /label=nucleoplasmin NLS /note="/Color=#6bba70" /note="/created_by=User" /note="/modified_by=User" CDS 6588..6623 /label=Gly-Ser linker /note="/Color=#ff7400" /note="/created_by=User" /note="/modified_by=User" CDS 6624..6872 /label=UGI /note="/Color=#9999ff" /note="/created_by=User" /note="/modified_by=User" CDS 6873..6884 /label=Gly-Ser linker /note="/Color=#ff7400" /note="/created_by=User" /note="/modified_by=User" CDS 6885..6905 /codon_start=1 /product="nuclear localization signal of SV40 large T antigen" /label=SV40 NLS /note="/Color=#6bba70" /note="/modified_by=User" /translation="PKKKRKV" terminator 7060..7115 /label=-beta-globin poly(A) signal /note="/created_by=User" /note="/modified_by=User" promoter complement(7525..7554) /label=lac_promoter /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT YGKLT LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MVLLEFVTAAGITLGMDELYK*" promoter 7660..7839 /label=SV40_promoter /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT YGKLT LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MVLLEFVTAAGITLGMDELYK*" rep_origin 7695..7772 /label=SV40_origin /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT YGKLT LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MVLLEFVTAAGITLGMDELYK*" terminator 7853..7984 /label=SV40_PA_terminator /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT YGKLT LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MVLLEFVTAAGITLGMDELYK*" rep_origin complement(8208..8827) /direction=LEFT /label=pBR322_origin /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT YGKLT LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MVLLEFVTAAGITLGMDELYK*" gene complement(8982..9842) /gene="Ampicillin" /label=Ampicillin /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT YGKLT LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MVLLEFVTAAGITLGMDELYK*" promoter complement(9884..9912) /label=AmpR_promoter /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG ARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" ORIGIN 1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata 61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc 121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag 181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac 241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg 301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg 361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca 421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag 481 cgatgggggc gggggggggg ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg 541 ggcggggcga ggcggagagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt 601 ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg 661 ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg 721 ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctcct 781 ccgggctgta attagcgctt ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa 841 agccttgagg ggctccggga gggccctttg tgcgggggga gcggctcggg gggtgcgtgc 901 gtgtgtgtgt gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg ctgtgagcgc 961 tgcgggcgcg gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag cgcggccggg 1021 ggcggtgccc cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg 1081 tgcgtggggg ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc ccccctgcac 1141 ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg tacggggcgt 1201 ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg 1261 gggccgcctc gggccgggga gggctcgggg gaggggcgcg gcggcccccg gagcgccggc 1321 ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg 1381 cagggacttc ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc 1441 ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag 1501 ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg gggctgtccg 1561 cggggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg 1621 accggcggct ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc 1681 ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt ctgcagtcga 1741 cggtaccgcg ggcccgggat ccaccggtcg ccaccatggg cccaaagaaa aagaggaaag 1801 tcggcagtgg acctaaaaag aaacgaaagg ttgggtcagg tagctcagag actggcccag 1861 tggctgtgga ccccacattg agacggcgga tcgagcccca tgagtttgag gtattcttcg 1921 atccgagaga gctccgcaag gagacctgcc tgctttacga aattaattgg gggggccggc 1981 actccatttg gcgacataca tcacagaaca ctaacaagca cgtcgaagtc aacttcatcg 2041 agaagttcac gacagaaaga tatttctgtc cgaacacaag gtgcagcatt acctggtttc 2101 tcagctggag cccatgcggc gaatgtagta gggccatcac tgaattcctg tcaaggtatc 2161 cccacgtcac tctgtttatt tacatcgcaa ggctgtacca ccacgctgac ccccgcaatc 2221 gacaaggcct gcgggatttg atctcttcag gtgtgactat ccaaattatg actgagcagg 2281 agtcaggata ctgctggaga aactttgtga attatagccc gagtaatgaa gcccactggc 2341 ctaggtatcc ccatctgtgg gtacgactgt acgttcttga actgtactgc atcatactgg 2401 gcctgcctcc ttgtctcaac attctgagaa ggaagcagcc acagctgaca ttctttacca 2461 tcgctcttca gtcttgtcat taccagcgac tgcccccaca cattctctgg gccaccgggt 2521 tgaaatctgg tggttcttct ggtggttcta gcggcagcga gactcccggg acctcagagt 2581 ccgccacacc cgaaagttcc ggagggagta gcggcgggtc tacacagttc gagggcttta 2641 ccaacctgta tcaggtgagc aagacactgc ggtttgagct gatcccacag ggcaagaccc 2701 tgaagcacat ccaggagcag ggcttcatcg aggaggacaa ggcccgcaat gatcactaca 2761 aggagctgaa gcccatcatc gatcggatct acaagaccta tgccgaccag tgcctgcagc 2821 tggtgcagct ggattgggag aacctgagcg ccgccatcga ctcctataga aaggagaaaa 2881 ccgaggagac aaggaacgcc ctgatcgagg agcaggccac atatcgcaat gccatccacg 2941 actacttcat cggccggaca gacaacctga ccgatgccat caataagaga cacgccgaga 3001 tctacaaggg cctgttcaag gccgagctgt ttaatggcaa ggtgctgaag cagctgggca 3061 ccgtgaccac aaccgagcac gagaacgccc tgctgcggag cttcgacaag tttacaacct 3121 acttctccgg cttttataga aacaggaaga acgtgttcag cgccgaggat atcagcacag 3181 ccatcccaca ccgcatcgtg caggacaact tccccaagtt taaggagaat tgtcacatct 3241 tcacacgcct gatcaccgcc gtgcccagcc tgcgggagca ctttgagaac gtgaagaagg 3301 ccatcggcat cttcgtgagc acctccatcg aggaggtgtt ttccttccct ttttataacc 3361 agctgctgac acagacccag atcgacctgt ataaccagct gctgggagga atctctcggg 3421 aggcaggcac cgagaagatc aagggcctga acgaggtgct gaatctggcc atccagaaga 3481 atgatgagac agcccacatc atcgcctccc tgccacacag attcatcccc ctgtttaagc 3541 agatcctgtc cgataggaac accctgtctt tcatcctgga ggagtttaag agcgacgagg 3601 aagtgatcca gtccttctgc aagtacaaga cactgctgag aaacgagaac gtgctggaga 3661 cagccgaggc cctgtttaac gagctgaaca gcatcgacct gacacacatc ttcatcagcc 3721 acaagaagct ggagacaatc agcagcgccc tgtgcgacca ctgggataca ctgaggaatg 3781 ccctgtatga gcggagaatc tccgagctga caggcaagat caccaagtct gccaaggaga 3841 aggtgcagcg cagcctgaag cacgaggata tcaacctgca ggagatcatc tctgccgcag 3901 gcaaggagct gagcgaggcc ttcaagcaga aaaccagcga gatcctgtcc cacgcacacg 3961 ccgccctgga tcagccactg cctacaaccc tgaagaagca ggaggagaag gagatcctga 4021 agtctcagct ggacagcctg ctgggcctgt accacctgct ggactggttt gccgtggatg 4081 agtccaacga ggtggacccc gagttctctg cccggctgac cggcatcaag ctggagatgg 4141 agccttctct gagcttctac aacaaggcca gaaattatgc caccaagaag ccctactccg 4201 tggagaagtt caagctgaac tttcagatgc ctacactggc cagaggctgg gacgtgaata 4261 gagagaagaa caatggcgcc atcctgtttg tgaagaacgg cctgtactat ctgggcatca 4321 tgccaaagca gaagggcagg tataaggccc tgagcttcga gcccacagag aaaaccagcg 4381 agggctttga taagatgtac tatgactact tccctgatgc cgccaagatg atcccaaagt 4441 gcagcaccca gctgaaggcc gtgacagccc actttcagac ccacacaacc cccatcctgc 4501 tgtccaacaa tttcatcgag cctctggaga tcacaaagga gatctacgac ctgaacaatc 4561 ctgagaagga gccaaagaag tttcagacag cctacgccaa gaaaaccggc gaccagaagg 4621 gctacagaga ggccctgtgc aagtggatcg acttcacaag ggattttctg tccaagtata 4681 ccaagacaac ctctatcgat ctgtctagcc tgcggccatc ctctcagtat aaggacctgg 4741 gcgagtacta tgccgagctg aatcccctgc tgtaccacat cagcttccag agaatcgccg 4801 agaaggagat catggatgcc gtggagacag gcaagctgta cctgttccag atctataaca 4861 aggactttgc caagggccac cacggcaagc ctaatctgca cacactgtat tggaccggcc 4921 tgttttctcc agagaacctg gccaagacaa gcatcaagct gaatggccag gccgagctgt 4981 tctaccgccc taagtccagg atgaagagga tggcacaccg gctgggagag aagatgctga 5041 acaagaagct gaaggatcag aaaaccccaa tccccgacac cctgtaccag gagctgtacg 5101 actatgtgaa tcacagactg tcccacgacc tgtctgatga ggccagggcc ctgctgccca 5161 acgtgatcac caaggaggtg tctcacgaga tcatcaagga taggcgcttt accagcgaca 5221 agttcttttt ccacgtgcct atcacactga actatcaggc cgccaattcc ccatctaagt 5281 tcaaccagag ggtgaatgcc tacctgaagg agcaccccga gacacctatc atcggcatcg 5341 cccggggcga gagaaacctg atctatatca cagtgatcga ctccaccggc aagatcctgg 5401 agcagcggag cctgaacacc atccagcagt ttgattacca gaagaagctg gacaacaggg 5461 agaaggagag ggtggcagca aggcaggcct ggtctgtggt gggcacaatc aaggatctga 5521 agcagggcta tctgagccag gtcatccacg agatcgtgga cctgatgatc cactaccagg 5581 ccgtggtggt gctggagaac ctgaatttcg gctttaagag caagaggacc ggcatcgccg 5641 agaaggccgt gtaccagcag ttcgagaaga tgctgatcga taagctgaat tgcctggtgc 5701 tgaaggacta tccagcagag aaagtgggag gcgtgctgaa cccataccag ctgacagacc 5761 agttcacctc ctttgccaag atgggcaccc agtctggctt cctgttttac gtgcctgccc 5821 catatacatc taagatcgat cccctgaccg gcttcgtgga ccccttcgtg tggaaaacca 5881 tcaagaatca cgagagccgc aagcacttcc tggagggctt cgactttctg cactacgacg 5941 tgaaaaccgg cgacttcatc ctgcacttta agatgaacag aaatctgtcc ttccagaggg 6001 gcctgcccgg ctttatgcct gcatgggata tcgtgttcga gaagaacgag acacagtttg 6061 acgccaaggg cacccctttc atcgccggca agagaatcgt gccagtgatc gagaatcaca 6121 gattcaccgg cagataccgg gacctgtatc ctgccaacga gctgatcgcc ctgctggagg 6181 agaagggcat cgtgttcagg gatggctcca acatcctgcc aaagctgctg gagaatgacg 6241 attctcacgc catcgacacg atggtggccc tgatccgcag cgtgctgcag atgcggaact 6301 ccaatgccgc cacaggcgag gactatatca acagccccgt gcgcgatctg aatggcgtgt 6361 gcttcgactc ccggtttcag aacccagagt ggccaatgga cgccgatgcc aatggcgcct 6421 accacatcgc cctgaagggc cagctgctgc tgaatcacct gaaggagagc aaggatctga 6481 agctgcagaa cggcatctcc aatcaggact ggctggccta catccaggag ctgcgcaaca 6541 aaaggccggc ggccacgaaa aaggccggcc aggcaaaaaa gaaaaaggga tcctctggtg 6601 gttctggagg atctggtggt tctactaatc tgtcagatat tattgaaaag gagaccggta 6661 agcaactggt tatccaggaa tccatcctca tgctcccaga ggaggtggaa gaagtcattg 6721 ggaacaagcc ggaaagcgat atactcgtgc acaccgccta cgacgagagc accgacgaga 6781 atgtcatgct tctgactagc gacgcccctg aatacaagcc ttgggctctg gtcatacagg 6841 atagcaacgg tgagaacaag attaagatgc tctctggtgg ttctcccaag aagaagagga 6901 aagtctaaag cggccgcact cctcaggtgc aggctgccta tcagaaggtg gtggctggtg 6961 tggccaatgc cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg 7021 gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca 7081 ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa 7141 atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc 7201 tggctgccat gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg 7261 ctgtccattc cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt 7321 gttttgtgtt atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca 7381 gatttttcct cctctcctga ctactcccag tcatagctgt ccctcttctc ttatggagat 7441 ccctcgacct gcagcccaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat 7501 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg 7561 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag 7621 tcgggaaacc tgtcgtgcca gcggatccgc atctcaatta gtcagcaacc atagtcccgc 7681 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg 7741 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc 7801 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagcta acttgtttat 7861 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt 7921 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg 7981 gatccgctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc 8041 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta 8101 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag 8161 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg 8221 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg 8281 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg 8341 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga 8401 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc 8461 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt 8521 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact 8581 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg 8641 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt 8701 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt 8761 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct 8821 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg 8881 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt 8941 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt 9001 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc 9061 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg 9121 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc 9181 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg 9241 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca 9301 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga 9361 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct 9421 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg 9481 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca 9541 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata 9601 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct 9661 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact 9721 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa 9781 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc 9841 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga 9901 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga 9961 aaagtgccac ctg //