LOCUS       Exported                9859 bp ds-DNA     circular SYN 29-AUG-2018
DEFINITION  pCAG-CFP.
ACCESSION   urn.local...r-98sgkip
VERSION     .
KEYWORDS    pCAG-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)...
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 29, 2018 from SnapGene 4.2.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9859
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        4..383
                     /label=CMV Enhancer
                     /note="/Color=#ffbb33"
                     /note="/created_by=User"
                     /note="/modified_by=User"
     promoter        385..660
                     /label=chicken beta-actin promoter
                     /note="/created_by=User"
                     /note="/modified_by=User"
     promoter        661..1677
                     /label=chimeric intron
                     /note="/Color=#eeeeee"
                     /note="/created_by=User"
                     /note="/modified_by=User"
     CDS             1776..2465
                     /label=rAPOBEC1
                     /note="/Color=#ff7777"
                     /note="/created_by=User"
                     /note="/modified_by=User"
     CDS             1779..1781
                     /label=Inserted Gly for NcoI site
                     /note="/created_by=User"
     CDS             2466..2489
                     /label=Gly-Ser linker
                     /note="/Color=#ff7400"
                     /note="/created_by=User"
     CDS             2490..2537
                     /label=XTEN linker
                     /note="/Color=#ff9911"
                     /note="/created_by=User"
                     /note="/modified_by=User"
     CDS             2538..2561
                     /label=Gly-Ser linker
                     /note="/Color=#ff7400"
                     /note="/created_by=User"
     CDS             2562..6479
                     /codon_start=1
                     /label=human codon optimized DNase inactive (D908A) en
                     /label=human codon optimized DNase inactive (D908A) 
                     enAsCas12a(E174R/S542R/K548R)
                     /note="/modified_by=User"
                     /translation="MQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARNDH
                     YKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRNA
                     IHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFDK
                     FTTYFSGFYRNRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHFE
                     NVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVLN
                     LAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLLR
                     NENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTGK
                     ITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTLK
                     KQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKAR
                     NYATKKPYSVEKFKLNFQMPTLARGWDVNREKNNGAILFVKNGLYYLGIMPKQKGRYKA
                     LSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEPL
                     EITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSIDL
                     SSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKGH
                     HGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLKD
                     QKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFFH
                     VPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIARGERNLIYITVIDSTGKILEQRS
                     LNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAVV
                     VLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQF
                     TSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYDV
                     KTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIENH
                     RFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQMR
                     NSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKESK
                     DLKLQNGISNQDWLAYIQELRN"
     misc_feature    3078..3080
                     /label=E174R
                     /label=nonstandard type: misc_marker
                     /note="/created_by=User"
     misc_feature    4182..4184
                     /label=S542R
                     /label=nonstandard type: misc_marker
                     /note="/created_by=User"
     misc_feature    4200..4202
                     /label=K548R
                     /label=nonstandard type: misc_marker
                     /note="/created_by=User"
                     /note="/modified_by=User"
     misc_difference 5280..5282
                     /label=D908A
                     /note="/created_by=User"
     misc_feature    6199..6204
                     /label=silent mutation to remove NcoI site
                     /label=nonstandard type: misc_marker
                     /note="/created_by=User"
                     /note="/modified_by=User"
     misc_feature    6334..6339
                     /label=silent mutation to remove NcoI site
                     /label=nonstandard type: misc_marker
                     /note="/created_by=User"
                     /note="/modified_by=User"
     CDS             6480..6509
                     /label=Gly-Ser linker
                     /note="/Color=#ff7400"
                     /note="/created_by=User"
                     /note="/modified_by=User"
     CDS             6510..6758
                     /label=UGI
                     /note="/Color=#9999ff"
                     /note="/created_by=User"
                     /note="/modified_by=User"
     CDS             6759..6770
                     /label=Gly-Ser linker
                     /note="/Color=#ff7400"
                     /note="/created_by=User"
                     /note="/modified_by=User"
     CDS             6771..6791
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /note="/Color=#6bba70"
                     /note="/modified_by=User"
                     /translation="PKKKRKV"
     terminator      6946..7001
                     /label=-beta-globin poly(A) signal
                     /note="/created_by=User"
                     /note="/modified_by=User"
     promoter        complement(7411..7440)
                     /label=lac_promoter
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN 
                     GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYK*"
     promoter        7546..7725
                     /label=SV40_promoter
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN 
                     GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYK*"
     rep_origin      7581..7658
                     /label=SV40_origin
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN 
                     GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYK*"
     terminator      7739..7870
                     /label=SV40_PA_terminator
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN 
                     GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYK*"
     rep_origin      complement(8094..8713)
                     /direction=LEFT
                     /label=pBR322_origin
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN 
                     GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYK*"
     gene            complement(8868..9728)
                     /gene="Ampicillin"
                     /label=Ampicillin
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN 
                     GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYK*"
     promoter        complement(9770..9798)
                     /label=AmpR_promoter
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggg ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg
      541 ggcggggcga ggcggagagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt
      601 ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg
      661 ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg
      721 ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctcct
      781 ccgggctgta attagcgctt ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa
      841 agccttgagg ggctccggga gggccctttg tgcgggggga gcggctcggg gggtgcgtgc
      901 gtgtgtgtgt gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg ctgtgagcgc
      961 tgcgggcgcg gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag cgcggccggg
     1021 ggcggtgccc cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg
     1081 tgcgtggggg ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc ccccctgcac
     1141 ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg tacggggcgt
     1201 ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg
     1261 gggccgcctc gggccgggga gggctcgggg gaggggcgcg gcggcccccg gagcgccggc
     1321 ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg
     1381 cagggacttc ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc
     1441 ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag
     1501 ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg gggctgtccg
     1561 cggggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg
     1621 accggcggct ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc
     1681 ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt ctgcagtcga
     1741 cggtaccgcg ggcccgggat ccaccggtcg ccaccatggg cagctcagag actggcccag
     1801 tggctgtgga ccccacattg agacggcgga tcgagcccca tgagtttgag gtattcttcg
     1861 atccgagaga gctccgcaag gagacctgcc tgctttacga aattaattgg gggggccggc
     1921 actccatttg gcgacataca tcacagaaca ctaacaagca cgtcgaagtc aacttcatcg
     1981 agaagttcac gacagaaaga tatttctgtc cgaacacaag gtgcagcatt acctggtttc
     2041 tcagctggag cccatgcggc gaatgtagta gggccatcac tgaattcctg tcaaggtatc
     2101 cccacgtcac tctgtttatt tacatcgcaa ggctgtacca ccacgctgac ccccgcaatc
     2161 gacaaggcct gcgggatttg atctcttcag gtgtgactat ccaaattatg actgagcagg
     2221 agtcaggata ctgctggaga aactttgtga attatagccc gagtaatgaa gcccactggc
     2281 ctaggtatcc ccatctgtgg gtacgactgt acgttcttga actgtactgc atcatactgg
     2341 gcctgcctcc ttgtctcaac attctgagaa ggaagcagcc acagctgaca ttctttacca
     2401 tcgctcttca gtcttgtcat taccagcgac tgcccccaca cattctctgg gccaccgggt
     2461 tgaaatctgg tggttcttct ggtggttcta gcggcagcga gactcccggg acctcagagt
     2521 ccgccacacc cgaaagttcc ggagggagta gcggcgggtc tacacagttc gagggcttta
     2581 ccaacctgta tcaggtgagc aagacactgc ggtttgagct gatcccacag ggcaagaccc
     2641 tgaagcacat ccaggagcag ggcttcatcg aggaggacaa ggcccgcaat gatcactaca
     2701 aggagctgaa gcccatcatc gatcggatct acaagaccta tgccgaccag tgcctgcagc
     2761 tggtgcagct ggattgggag aacctgagcg ccgccatcga ctcctataga aaggagaaaa
     2821 ccgaggagac aaggaacgcc ctgatcgagg agcaggccac atatcgcaat gccatccacg
     2881 actacttcat cggccggaca gacaacctga ccgatgccat caataagaga cacgccgaga
     2941 tctacaaggg cctgttcaag gccgagctgt ttaatggcaa ggtgctgaag cagctgggca
     3001 ccgtgaccac aaccgagcac gagaacgccc tgctgcggag cttcgacaag tttacaacct
     3061 acttctccgg cttttataga aacaggaaga acgtgttcag cgccgaggat atcagcacag
     3121 ccatcccaca ccgcatcgtg caggacaact tccccaagtt taaggagaat tgtcacatct
     3181 tcacacgcct gatcaccgcc gtgcccagcc tgcgggagca ctttgagaac gtgaagaagg
     3241 ccatcggcat cttcgtgagc acctccatcg aggaggtgtt ttccttccct ttttataacc
     3301 agctgctgac acagacccag atcgacctgt ataaccagct gctgggagga atctctcggg
     3361 aggcaggcac cgagaagatc aagggcctga acgaggtgct gaatctggcc atccagaaga
     3421 atgatgagac agcccacatc atcgcctccc tgccacacag attcatcccc ctgtttaagc
     3481 agatcctgtc cgataggaac accctgtctt tcatcctgga ggagtttaag agcgacgagg
     3541 aagtgatcca gtccttctgc aagtacaaga cactgctgag aaacgagaac gtgctggaga
     3601 cagccgaggc cctgtttaac gagctgaaca gcatcgacct gacacacatc ttcatcagcc
     3661 acaagaagct ggagacaatc agcagcgccc tgtgcgacca ctgggataca ctgaggaatg
     3721 ccctgtatga gcggagaatc tccgagctga caggcaagat caccaagtct gccaaggaga
     3781 aggtgcagcg cagcctgaag cacgaggata tcaacctgca ggagatcatc tctgccgcag
     3841 gcaaggagct gagcgaggcc ttcaagcaga aaaccagcga gatcctgtcc cacgcacacg
     3901 ccgccctgga tcagccactg cctacaaccc tgaagaagca ggaggagaag gagatcctga
     3961 agtctcagct ggacagcctg ctgggcctgt accacctgct ggactggttt gccgtggatg
     4021 agtccaacga ggtggacccc gagttctctg cccggctgac cggcatcaag ctggagatgg
     4081 agccttctct gagcttctac aacaaggcca gaaattatgc caccaagaag ccctactccg
     4141 tggagaagtt caagctgaac tttcagatgc ctacactggc cagaggctgg gacgtgaata
     4201 gagagaagaa caatggcgcc atcctgtttg tgaagaacgg cctgtactat ctgggcatca
     4261 tgccaaagca gaagggcagg tataaggccc tgagcttcga gcccacagag aaaaccagcg
     4321 agggctttga taagatgtac tatgactact tccctgatgc cgccaagatg atcccaaagt
     4381 gcagcaccca gctgaaggcc gtgacagccc actttcagac ccacacaacc cccatcctgc
     4441 tgtccaacaa tttcatcgag cctctggaga tcacaaagga gatctacgac ctgaacaatc
     4501 ctgagaagga gccaaagaag tttcagacag cctacgccaa gaaaaccggc gaccagaagg
     4561 gctacagaga ggccctgtgc aagtggatcg acttcacaag ggattttctg tccaagtata
     4621 ccaagacaac ctctatcgat ctgtctagcc tgcggccatc ctctcagtat aaggacctgg
     4681 gcgagtacta tgccgagctg aatcccctgc tgtaccacat cagcttccag agaatcgccg
     4741 agaaggagat catggatgcc gtggagacag gcaagctgta cctgttccag atctataaca
     4801 aggactttgc caagggccac cacggcaagc ctaatctgca cacactgtat tggaccggcc
     4861 tgttttctcc agagaacctg gccaagacaa gcatcaagct gaatggccag gccgagctgt
     4921 tctaccgccc taagtccagg atgaagagga tggcacaccg gctgggagag aagatgctga
     4981 acaagaagct gaaggatcag aaaaccccaa tccccgacac cctgtaccag gagctgtacg
     5041 actatgtgaa tcacagactg tcccacgacc tgtctgatga ggccagggcc ctgctgccca
     5101 acgtgatcac caaggaggtg tctcacgaga tcatcaagga taggcgcttt accagcgaca
     5161 agttcttttt ccacgtgcct atcacactga actatcaggc cgccaattcc ccatctaagt
     5221 tcaaccagag ggtgaatgcc tacctgaagg agcaccccga gacacctatc atcggcatcg
     5281 cccggggcga gagaaacctg atctatatca cagtgatcga ctccaccggc aagatcctgg
     5341 agcagcggag cctgaacacc atccagcagt ttgattacca gaagaagctg gacaacaggg
     5401 agaaggagag ggtggcagca aggcaggcct ggtctgtggt gggcacaatc aaggatctga
     5461 agcagggcta tctgagccag gtcatccacg agatcgtgga cctgatgatc cactaccagg
     5521 ccgtggtggt gctggagaac ctgaatttcg gctttaagag caagaggacc ggcatcgccg
     5581 agaaggccgt gtaccagcag ttcgagaaga tgctgatcga taagctgaat tgcctggtgc
     5641 tgaaggacta tccagcagag aaagtgggag gcgtgctgaa cccataccag ctgacagacc
     5701 agttcacctc ctttgccaag atgggcaccc agtctggctt cctgttttac gtgcctgccc
     5761 catatacatc taagatcgat cccctgaccg gcttcgtgga ccccttcgtg tggaaaacca
     5821 tcaagaatca cgagagccgc aagcacttcc tggagggctt cgactttctg cactacgacg
     5881 tgaaaaccgg cgacttcatc ctgcacttta agatgaacag aaatctgtcc ttccagaggg
     5941 gcctgcccgg ctttatgcct gcatgggata tcgtgttcga gaagaacgag acacagtttg
     6001 acgccaaggg cacccctttc atcgccggca agagaatcgt gccagtgatc gagaatcaca
     6061 gattcaccgg cagataccgg gacctgtatc ctgccaacga gctgatcgcc ctgctggagg
     6121 agaagggcat cgtgttcagg gatggctcca acatcctgcc aaagctgctg gagaatgacg
     6181 attctcacgc catcgacacg atggtggccc tgatccgcag cgtgctgcag atgcggaact
     6241 ccaatgccgc cacaggcgag gactatatca acagccccgt gcgcgatctg aatggcgtgt
     6301 gcttcgactc ccggtttcag aacccagagt ggccaatgga cgccgatgcc aatggcgcct
     6361 accacatcgc cctgaagggc cagctgctgc tgaatcacct gaaggagagc aaggatctga
     6421 agctgcagaa cggcatctcc aatcaggact ggctggccta catccaggag ctgcgcaact
     6481 ctggtggttc tggaggatct ggtggttcta ctaatctgtc agatattatt gaaaaggaga
     6541 ccggtaagca actggttatc caggaatcca tcctcatgct cccagaggag gtggaagaag
     6601 tcattgggaa caagccggaa agcgatatac tcgtgcacac cgcctacgac gagagcaccg
     6661 acgagaatgt catgcttctg actagcgacg cccctgaata caagccttgg gctctggtca
     6721 tacaggatag caacggtgag aacaagatta agatgctctc tggtggttct cccaagaaga
     6781 agaggaaagt ctaaagcggc cgcactcctc aggtgcaggc tgcctatcag aaggtggtgg
     6841 ctggtgtggc caatgccctg gctcacaaat accactgaga tctttttccc tctgccaaaa
     6901 attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa aggaaattta
     6961 ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga
     7021 gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca acatatgccc
     7081 atatgctggc tgccatgaac aaaggttggc tataaagagg tcatcagtat atgaaacagc
     7141 cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag atttttttta
     7201 tattttgttt tgtgttattt ttttctttaa catccctaaa attttcctta catgttttac
     7261 tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct cttctcttat
     7321 ggagatccct cgacctgcag cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg
     7381 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     7441 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     7501 ttccagtcgg gaaacctgtc gtgccagcgg atccgcatct caattagtca gcaaccatag
     7561 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     7621 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     7681 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctaactt
     7741 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     7801 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     7861 tgtctggatc cgctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     7921 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     7981 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     8041 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     8101 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     8161 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     8221 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     8281 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     8341 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     8401 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     8461 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     8521 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     8581 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     8641 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     8701 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     8761 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     8821 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     8881 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     8941 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     9001 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     9061 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     9121 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     9181 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     9241 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     9301 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     9361 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     9421 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     9481 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     9541 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     9601 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     9661 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     9721 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     9781 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     9841 ccccgaaaag tgccacctg
//
