LOCUS       Exported                6952 bp ds-DNA     circular SYN 14-JUL-2018
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6952)
  AUTHORS   Hochgeschwender
  TITLE     Direct Submission
  JOURNAL   Exported Jul 21, 2018 from SnapGene 4.1.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6952
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   5..145
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     promoter        190..637
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             708..1262
                     /codon_start=1
                     /product="enhanced variant of secreted Gaussia luciferase"
                     /label=Gaussia luciferase
                     /note="human codon-optimized"
                     /note="This feature has 2 segments:
                        1:  708 ..  758 / #ff0000 / signal sequence
                        2:  759 .. 1262 / #00ccff / sbGLuc"
                     /translation="MGVKVLFALICIAVAEAKPTENNEDFNIVAVASNFATTDLDADRG
                     KLPGKKLPLEVLKELEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESA
                     QGGIGEAIVDIPEIPGFKDLEPLEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQ
                     RCATFASKIQGQVDKIKGAGGD"
     CDS             1263..1307
                     /codon_start=1
                     /label=15 aa Linker
                     /note="color: #993300"
                     /translation="GDPLVQCGGIAGSAT"
     CDS             1308..2207
                     /codon_start=1
                     /label=VChR1
                     /note="color: #993366"
                     /translation="MDYPVARSLIVRYPTDLGNGTVCMPRGQCYCEGWLRSRGTSIEKT
                     IAITLQWVVFALSVACLGWYAYQAWRATCGWEEVYVALIEMMKSIIEAFHEFDSPATLW
                     LSSGNGVVWMRYGEWLLTCPVLLIHLSNLTGLKDDYSKRTMGLLVSDVGCIVWGATSAM
                     CTGWTKILFFLISLSYGMYTYFHAAKVYIEAFHTVPKGICRELVRVMAWTFFVAWGMFP
                     VLFLLGTEGFGHISPYGSAIGHSILDLIAKNMWGVLGNYLRVKIHEHILLYGDIRKKQK
                     ITIAGQEMEVETLVAEEED"
     CDS             2217..2276
                     /codon_start=1
                     /label=TS
                     /note="color: #ff6600"
                     /translation="KSRITSEGEYIPLDQIDINV"
     CDS             2277..2990
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /note="This feature has 2 segments:
                        1: 2277 .. 2279 / #e1ff2f / 1a
                        2: 2280 .. 2990 / #e1ff2f"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             2991..3011
                     /codon_start=1
                     /label=ER
                     /note="color: #ff9900"
                     /translation="FCYENEV"
     CDS             3035..3052
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /note="color: #cc99b2"
                     /translation="HHHHHH"
     misc_feature    3083..3671
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     polyA_signal    3703..4179
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     repeat_region   4219..4359
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     rep_origin      4434..4889
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        5171..5275
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             5276..6136
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 5276 .. 5344 / #ccffcc / signal sequence
                        2: 5345 .. 6136 / #ccffcc
                     Cleavage site after base 5344"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6307..6895
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
ORIGIN
        1 atgtcctgca ggcagctgcg cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg
       61 cgtcgggcga cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg
      121 gccaactcca tcactagggg ttcctgcggc cgcacgcgtg tgtctagact gcagagggcc
      181 ctgcgtatga gtgcaagtgg gttttaggac caggatgagg cggggtgggg gtgcctacct
      241 gacgaccgac cccgacccac tggacaagca cccaaccccc attccccaaa ttgcgcatcc
      301 cctatcagag agggggaggg gaaacaggat gcggcgaggc gcgtgcgcac tgccagcttc
      361 agcaccgcgg acagtgcctt cgcccccgcc tggcggcgcg cgccaccgcc gcctcagcac
      421 tgaaggcgcg ctgacgtcac tcgccggtcc cccgcaaact ccccttcccg gccaccttgg
      481 tcgcgtccgc gccgccgccg gcccagccgg accgcaccac gcgaggcgcg agataggggg
      541 gcacgggcgc gaccatctgc gctgcggcgc cggcgactca gcgctgcctc agtctgcggt
      601 gggcagcgga ggagtcgtgt cgtgcctgag agcgcagtcg agaaggtacc ggatcctcta
      661 gagtcgagct caccggtagc tggcgcgcca ccggtaagct tgccaccatg ggagtcaaag
      721 ttctgtttgc cctgatctgc atcgctgtgg ccgaggccaa gcccaccgag aacaacgaag
      781 acttcaacat cgtggccgtg gccagcaact tcgcgaccac ggatctcgat gctgaccgcg
      841 ggaagttgcc cggcaagaag ctgccgctgg aggtgctcaa agagctggaa gccaatgccc
      901 ggaaagctgg ctgcaccagg ggctgtctga tctgcctgtc ccacatcaag tgcacgccca
      961 agatgaagaa gttcatccca ggacgctgcc acacctacga aggcgacaaa gagtccgcac
     1021 agggcggcat aggcgaggcg atcgtcgaca ttcctgagat tcctgggttc aaggacttgg
     1081 agcccctgga gcagttcatc gcacaggtcg atctgtgtgt ggactgcaca actggctgcc
     1141 tcaaagggct tgccaacgtg cagtgttctg acctgctcaa gaagtggctg ccgcaacgct
     1201 gtgcgacctt tgccagcaag atccagggcc aggtggacaa gatcaagggg gccggtggtg
     1261 acggcgaccc actagtccag tgtggtggaa ttgccggatc cgccaccatg gattaccctg
     1321 ttgctagaag cctcatagtt cgctacccaa ccgacctcgg aaacggcacc gtctgcatgc
     1381 caagaggaca gtgttactgt gaaggttggc ttcggagtcg cggcacttcc attgaaaaga
     1441 caatagcaat tactcttcag tgggtagtct ttgctttgtc agtggcttgc ctggggtggt
     1501 atgcgtatca agcgtggcga gctacctgcg gatgggagga ggtttacgta gccttgatag
     1561 aaatgatgaa aagcatcatc gaggccttcc acgagttcga cagccctgca acactgtggc
     1621 tgtcttcagg gaacggcgta gtttggatgc ggtatggcga atggctcctc acctgcccgg
     1681 tccttctgat ccatctgagc aacctcacag gcctgaagga cgattatagc aaaaggacta
     1741 tgggcctgtt ggtttctgat gtgggatgca tcgtgtgggg cgcaaccagc gccatgtgta
     1801 cggggtggac gaagatcctg ttcttcctca tctcattgag ctatggtatg tatacctatt
     1861 ttcatgctgc taaagtttat atcgaagcat tccacacagt tccaaaaggg atttgtcgag
     1921 aactggtccg agtgatggcc tggacattct ttgtggcttg gggaatgttt ccagtcctgt
     1981 ttctgctggg cacggaagga ttcggtcata tcagccctta tggatctgcc attgggcact
     2041 ccatcctcga cctgattgca aagaacatgt ggggtgtgct ggggaattac ctgcgcgtca
     2101 aaatccacga gcacatcctg ttgtatggcg acatcagaaa gaagcagaaa attacgatcg
     2161 ccggccaaga gatggaggtt gagacactgg tggctgaaga ggaggacgcg gccgccaaga
     2221 gcaggatcac cagcgagggc gagtacatcc ccctggacca gatcgacatc aacgtggtga
     2281 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     2341 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     2401 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     2461 ccaccttcgg ctacggcctg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg
     2521 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     2581 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     2641 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     2701 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     2761 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     2821 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     2881 gctaccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     2941 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag ttctgctacg
     3001 agaacgaggt gtaatgagct agccaattgc cggtcatcat caccatcacc attgagttta
     3061 attcgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac
     3121 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     3181 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     3241 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     3301 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     3361 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     3421 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     3481 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     3541 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     3601 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     3661 cgcctccccg catcgatacc gagcgctgct cgagagatct acgggtggca tccctgtgac
     3721 ccctccccag tgcctctcct ggccctggaa gttgccactc cagtgcccac cagccttgtc
     3781 ctaataaaat taagttgcat cattttgtct gactaggtgt ccttctataa tattatgggg
     3841 tggagggggg tggtatggag caaggggcaa gttgggaaga caacctgtag ggcctgcggg
     3901 gtctattggg aaccaagctg gagtgcagtg gcacaatctt ggctcactgc aatctccgcc
     3961 tcctgggttc aagcgattct cctgcctcag cctcccgagt tgttgggatt ccaggcatgc
     4021 atgaccaggc tcagctaatt tttgtttttt tggtagagac ggggtttcac catattggcc
     4081 aggctggtct ccaactccta atctcaggtg atctacccac cttggcctcc caaattgctg
     4141 ggattacagg cgtgaaccac tgctcccttc cctgtccttc tgattttgta ggtaaccacg
     4201 tgcggaccga gcggccgcag gaacccctag tgatggagtt ggccactccc tctctgcgcg
     4261 ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg
     4321 cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc
     4381 tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat agtacgcgcc
     4441 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     4501 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     4561 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     4621 acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc
     4681 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     4741 gttccaaact ggaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat
     4801 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     4861 ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa tctgctctga
     4921 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     4981 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     5041 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
     5101 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     5161 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     5221 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     5281 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     5341 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     5401 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     5461 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     5521 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     5581 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     5641 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     5701 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     5761 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     5821 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     5881 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     5941 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     6001 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     6061 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     6121 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     6181 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     6241 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     6301 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     6361 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     6421 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     6481 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     6541 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     6601 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     6661 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     6721 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     6781 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     6841 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     6901 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc ac
//
