LOCUS       Exported                7646 bp ds-DNA     circular SYN 14-JUL-2018
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7646)
  AUTHORS   Hochgeschwender
  TITLE     Direct Submission
  JOURNAL   Exported Jul 22, 2018 from SnapGene 4.1.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7646
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   12..141
                     /label=AAV2 ITR
                     /note="color: #ffcc99"
     promoter        242..1420
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
                     /note="color: #ffffff; direction: RIGHT"
     intron          473..1411
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
                     /note="color: #------"
     protein_bind    1451..1484
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites."
                     /note="color: #31849b; direction: RIGHT"
     protein_bind    1535..1568
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
                     /note="color: #31849b; direction: RIGHT"
     CDS             complement(1595..1615)
                     /codon_start=1
                     /label=ER
                     /note="color: #ff9900"
                     /translation="FCYENEV"
     CDS             complement(1616..2329)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /note="This feature has 2 segments:
                        1: 1616 .. 2326 / #e1ff2f
                        2: 2327 .. 2329 / #e1ff2f / 1a"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             complement(1646..2329)
                     /codon_start=1
                     /label=Venus?C10
                     /note="color: #00ff00"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAG"
     CDS             complement(2330..2389)
                     /codon_start=1
                     /label=TS
                     /note="color: #ff6600"
                     /translation="KSRITSEGEYIPLDQIDINV"
     CDS             complement(2399..3298)
                     /codon_start=1
                     /label=VChR1
                     /note="color: #993366"
                     /translation="MDYPVARSLIVRYPTDLGNGTVCMPRGQCYCEGWLRSRGTSIEKT
                     IAITLQWVVFALSVACLGWYAYQAWRATCGWEEVYVALIEMMKSIIEAFHEFDSPATLW
                     LSSGNGVVWMRYGEWLLTCPVLLIHLSNLTGLKDDYSKRTMGLLVSDVGCIVWGATSAM
                     CTGWTKILFFLISLSYGMYTYFHAAKVYIEAFHTVPKGICRELVRVMAWTFFVAWGMFP
                     VLFLLGTEGFGHISPYGSAIGHSILDLIAKNMWGVLGNYLRVKIHEHILLYGDIRKKQK
                     ITIAGQEMEVETLVAEEED"
     CDS             complement(3299..3343)
                     /codon_start=1
                     /label=15 aa Linker
                     /note="color: #993300"
                     /translation="GDPLVQCGGIAGSAT"
     CDS             complement(3344..3898)
                     /codon_start=1
                     /product="enhanced variant of secreted Gaussia luciferase"
                     /label=Gaussia luciferase
                     /note="human codon-optimized"
                     /note="This feature has 2 segments:
                        1: 3344 .. 3847 / #00ccff / sbGLuc
                        2: 3848 .. 3898 / #ff0000 / signal peptide"
                     /translation="MGVKVLFALICIAVAEAKPTENNEDFNIVAVASNFATTDLDADRG
                     KLPGKKLPLEVLKELEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESA
                     QGGIGEAIVDIPEIPGFKDLEPLEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQ
                     RCATFASKIQGQVDKIKGAGGD"
     CDS             complement(3344..3847)
                     /codon_start=1
                     /label=sbGluc w/o atg
                     /note="color: #33cccc"
                     /translation="KPTENNEDFNIVAVASNFATTDLDADRGKLPGKKLPLEVLKELEA
                     NARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESAQGGIGEAIVDIPEIPGF
                     KDLEPLEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQRCATFASKIQGQVDKIK
                     GAGGD"
     CDS             complement(3344..3583)
                     /codon_start=1
                     /label=C-sbGluc
                     /note="color: #993366"
                     /translation="GGIGEAIVDIPEIPGFKDLEPLEQFIAQVDLCVDCTTGCLKGLAN
                     VQCSDLLKKWLPQRCATFASKIQGQVDKIKGAGGD"
     CDS             complement(3584..3847)
                     /codon_start=1
                     /label=N-sbGluc w/o signal sequence
                     /note="color: #800080"
                     /translation="KPTENNEDFNIVAVASNFATTDLDADRGKLPGKKLPLEVLKELEA
                     NARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESAQ"
     regulatory      3895..3904
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
     protein_bind    complement(3923..3956)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites."
                     /note="color: #31849b; direction: LEFT"
     protein_bind    complement(4007..4040)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
                     /note="color: #31849b; direction: LEFT"
     misc_feature    4065..4653
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     polyA_signal    4686..4797
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     repeat_region   4909..5038
                     /label=AAV2 ITR
                     /note="color: #ffcc99"
     rep_origin      5124..5579
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     rep_origin      5124..5579
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        5861..5965
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     promoter        5861..5965
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             5966..6826
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 5966 .. 6034 / #ccffcc / signal sequence
                        2: 6035 .. 6826 / #ccffcc
                     Cleavage site after base 6034"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             5966..6826
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 5966 .. 6034 / #ccffcc / signal sequence
                        2: 6035 .. 6826 / #ccffcc
                     Cleavage site after base 6034"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6997..7585
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     rep_origin      6997..7585
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtaagct ttgcaaagat ggataaagtt
      181 ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat
      241 tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
      301 gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag
      361 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
      421 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc
      481 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat
      541 tacttccacc tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg
      601 ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg
      661 gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc
      721 tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg
      781 gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc
      841 cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg
      901 agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc
      961 tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc
     1021 agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag
     1081 gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc
     1141 gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga
     1201 ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat
     1261 ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta
     1321 attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac
     1381 agtggttcaa agtttttttc ttccatttca ggtgtcgtga ggtaccggat cctctagagt
     1441 cgactccgga ataacttcgt ataggatact ttatacgaag ttatgcagaa tggtagctgg
     1501 attgtagctg ctattagcaa tatgaaacct cttaataact tcgtatagca tacattatac
     1561 gaagttatgg cgcgccgcta gctaattctc attacacctc gttctcgtag cagaacttgt
     1621 acagctcgtc catgccgaga gtgatcccgg cggcggtcac gaactccagc aggaccatgt
     1681 gatcgcgctt ctcgttgggg tctttgctca gggcggactg gtagctcagg tagtggttgt
     1741 cgggcagcag cacggggccg tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct
     1801 gcacgctgcc gtcctcgatg ttgtggcgga tcttgaagtt caccttgatg ccgttcttct
     1861 gcttgtcggc catgatatag acgttgtggc tgttgtagtt gtactccagc ttgtgcccca
     1921 ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc gatgcggttc accagggtgt
     1981 cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg
     2041 tgcgctcctg gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt
     2101 ggtcggggta gcgggcgaag cactgcaggc cgtagccgaa ggtggtcacg agggtgggcc
     2161 agggcacggg cagcttgccg gtggtgcaga tgaacttcag ggtcagcttg ccgtaggtgg
     2221 catcgccctc gccctcgccg gacacgctga acttgtggcc gtttacgtcg ccgtccagct
     2281 cgaccaggat gggcaccacc ccggtgaaca gctcctcgcc cttgctcacc acgttgatgt
     2341 cgatctggtc cagggggatg tactcgccct cgctggtgat cctgctcttg gcggccgcgt
     2401 cctcctcttc agccaccagt gtctcaacct ccatctcttg gccggcgatc gtaattttct
     2461 gcttctttct gatgtcgcca tacaacagga tgtgctcgtg gattttgacg cgcaggtaat
     2521 tccccagcac accccacatg ttctttgcaa tcaggtcgag gatggagtgc ccaatggcag
     2581 atccataagg gctgatatga ccgaatcctt ccgtgcccag cagaaacagg actggaaaca
     2641 ttccccaagc cacaaagaat gtccaggcca tcactcggac cagttctcga caaatccctt
     2701 ttggaactgt gtggaatgct tcgatataaa ctttagcagc atgaaaatag gtatacatac
     2761 catagctcaa tgagatgagg aagaacagga tcttcgtcca ccccgtacac atggcgctgg
     2821 ttgcgcccca cacgatgcat cccacatcag aaaccaacag gcccatagtc cttttgctat
     2881 aatcgtcctt caggcctgtg aggttgctca gatggatcag aaggaccggg caggtgagga
     2941 gccattcgcc ataccgcatc caaactacgc cgttccctga agacagccac agtgttgcag
     3001 ggctgtcgaa ctcgtggaag gcctcgatga tgcttttcat catttctatc aaggctacgt
     3061 aaacctcctc ccatccgcag gtagctcgcc acgcttgata cgcataccac cccaggcaag
     3121 ccactgacaa agcaaagact acccactgaa gagtaattgc tattgtcttt tcaatggaag
     3181 tgccgcgact ccgaagccaa ccttcacagt aacactgtcc tcttggcatg cagacggtgc
     3241 cgtttccgag gtcggttggg tagcgaacta tgaggcttct agcaacaggg taatccatgg
     3301 tggcggatcc ggcaattcca ccacactgga ctagtgggtc gccgtcacca ccggccccct
     3361 tgatcttgtc cacctggccc tggatcttgc tggcaaaggt cgcacagcgt tgcggcagcc
     3421 acttcttgag caggtcagaa cactgcacgt tggcaagccc tttgaggcag ccagttgtgc
     3481 agtccacaca cagatcgacc tgtgcgatga actgctccag gggctccaag tccttgaacc
     3541 caggaatctc aggaatgtcg acgatcgcct cgcctatgcc gccctgtgcg gactctttgt
     3601 cgccttcgta ggtgtggcag cgtcctggga tgaacttctt catcttgggc gtgcacttga
     3661 tgtgggacag gcagatcaga cagcccctgg tgcagccagc tttccgggca ttggcttcca
     3721 gctctttgag cacctccagc ggcagcttct tgccgggcaa cttcccgcgg tcagcatcga
     3781 gatccgtggt cgcgaagttg ctggccacgg ccacgatgtt gaagtcttcg ttgttctcgg
     3841 tgggcttggc ctcggccaca gcgatgcaga tcagggcaaa cagaactttg actcccatgg
     3901 tggcaagctt accggtgcta gcataacttc gtataaagta tcctatacga agttatttgc
     3961 cttaacccag aaattatcac tgttattctt tagaatggtg caaagaataa cttcgtataa
     4021 tgtatgctat acgaagttat gaattcgata tcaagcttat cgataatcaa cctctggatt
     4081 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     4141 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     4201 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     4261 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     4321 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     4381 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     4441 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctat gttgccacct
     4501 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     4561 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     4621 cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgagcgctg ctcgagagat
     4681 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     4741 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     4801 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     4861 ggattgggaa gacaatagca ggcatgcacg tgcggaccga gcggccgcag gaacccctag
     4921 tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa
     4981 aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct
     5041 gcctgcaggg gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     5101 gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     5161 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     5221 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     5281 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt
     5341 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     5401 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     5461 ctcgggctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     5521 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt
     5581 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
     5641 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     5701 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     5761 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     5821 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     5881 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     5941 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     6001 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     6061 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     6121 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     6181 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     6241 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     6301 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     6361 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     6421 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     6481 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     6541 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     6601 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     6661 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     6721 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     6781 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     6841 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     6901 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     6961 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     7021 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     7081 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     7141 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     7201 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     7261 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     7321 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     7381 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     7441 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     7501 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     7561 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     7621 cttttgctgg ccttttgctc acatgt
//
