LOCUS       Exported                6881 bp ds-DNA     circular SYN 14-JUL-2018
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6881)
  AUTHORS   Hochgeschwender
  TITLE     Direct Submission
  JOURNAL   Exported Jul 22, 2018 from SnapGene 4.1.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6881
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   5..145
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     promoter        190..637
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
                     /note="color: #ffffff; direction: RIGHT"
     regulatory      678..687
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
     CDS             684..1238
                     /codon_start=1
                     /product="enhanced variant of secreted Gaussia luciferase"
                     /label=Gaussia luciferase
                     /note="human codon-optimized"
                     /note="This feature has 2 segments:
                        1:  684 ..  734 / #ff0000 / signal sequence
                        2:  735 .. 1238 / #00ccff / sbGLuc"
                     /translation="MGVKVLFALICIAVAEAKPTENNEDFNIVAVASNFATTDLDADRG
                     KLPGKKLPLEVLKELEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESA
                     QGGIGEAIVDIPEIPGFKDLEPLEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQ
                     RCATFASKIQGQVDKIKGAGGD"
     CDS             1239..1283
                     /codon_start=1
                     /label=15 aa linker
                     /note="color: #993300"
                     /translation="GDPLVQCGGIALAAT"
     CDS             1284..2222
                     /codon_start=1
                     /label=ChR2(CS/DA)
                     /note="color: #3366ff"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTSPVILIHLSNLTGLSNDYSRRTMGLLVSAIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAVPAAA"
     CDS             2223..2939
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /note="This feature has 2 segments:
                        1: 2223 .. 2225 / #e1ff2f / 1a
                        2: 2226 .. 2939 / #e1ff2f"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             2223..2936
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /note="This feature has 2 segments:
                        1: 2223 .. 2225 / #e1ff2f / 1a
                        2: 2226 .. 2936 / #e1ff2f"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             2964..2981
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /note="color: #cc99b2"
                     /translation="HHHHHH"
     misc_feature    3012..3600
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     polyA_signal    3632..4108
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     repeat_region   4148..4288
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     rep_origin      4363..4818
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        5100..5204
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             5205..6065
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 5205 .. 5273 / #ccffcc / signal sequence
                        2: 5274 .. 6065 / #ccffcc
                     Cleavage site after base 5273"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6236..6824
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
ORIGIN
        1 atgtcctgca ggcagctgcg cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg
       61 cgtcgggcga cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg
      121 gccaactcca tcactagggg ttcctgcggc cgcacgcgtg tgtctagact gcagagggcc
      181 ctgcgtatga gtgcaagtgg gttttaggac caggatgagg cggggtgggg gtgcctacct
      241 gacgaccgac cccgacccac tggacaagca cccaaccccc attccccaaa ttgcgcatcc
      301 cctatcagag agggggaggg gaaacaggat gcggcgaggc gcgtgcgcac tgccagcttc
      361 agcaccgcgg acagtgcctt cgcccccgcc tggcggcgcg cgccaccgcc gcctcagcac
      421 tgaaggcgcg ctgacgtcac tcgccggtcc cccgcaaact ccccttcccg gccaccttgg
      481 tcgcgtccgc gccgccgccg gcccagccgg accgcaccac gcgaggcgcg agataggggg
      541 gcacgggcgc gaccatctgc gctgcggcgc cggcgactca gcgctgcctc agtctgcggt
      601 gggcagcgga ggagtcgtgt cgtgcctgag agcgcagtcg agaaggtacc ggatcctcta
      661 gagtcgagct caagcttgcc accatgggag tcaaagttct gtttgccctg atctgcatcg
      721 ctgtggccga ggccaagccc accgagaaca acgaagactt caacatcgtg gccgtggcca
      781 gcaacttcgc gaccacggat ctcgatgctg accgcgggaa gttgcccggc aagaagctgc
      841 cgctggaggt gctcaaagag ctggaagcca atgcccggaa agctggctgc accaggggct
      901 gtctgatctg cctgtcccac atcaagtgca cgcccaagat gaagaagttc atcccaggac
      961 gctgccacac ctacgaaggc gacaaagagt ccgcacaggg cggcataggc gaggcgatcg
     1021 tcgacattcc tgagattcct gggttcaagg acttggagcc cctggagcag ttcatcgcac
     1081 aggtcgatct gtgtgtggac tgcacaactg gctgcctcaa agggcttgcc aacgtgcagt
     1141 gttctgacct gctcaagaag tggctgccgc aacgctgtgc gacctttgcc agcaagatcc
     1201 agggccaggt ggacaagatc aagggggccg gtggtgacgg ggatccacta gtccagtgtg
     1261 gtggaattgc ccttgctgcc accatggact atggcggcgc tttgtctgcc gtcggacgcg
     1321 aacttttgtt cgttactaat cctgtggtgg tgaacgggtc cgtcctggtc cctgaggatc
     1381 aatgttactg tgccggatgg attgaatctc gcggcacgaa cggcgctcag accgcgtcaa
     1441 atgtcctgca gtggcttgca gcaggattca gcattttgct gctgatgttc tatgcctacc
     1501 aaacctggaa atctacatgc ggctgggagg agatctatgt gtgcgccatt gaaatggtta
     1561 aggtgattct cgagttcttt tttgagttta agaatccctc tatgctctac cttgccacag
     1621 gacaccgggt gcagtggctg cgctatgcag agtggctgct cacttctcct gtcatcctta
     1681 tccacctgag caacctcacc ggcctgagca acgactacag caggagaacc atgggactcc
     1741 ttgtctcagc catcgggact atcgtgtggg gggctaccag cgccatggca accggctatg
     1801 ttaaagtcat cttcttttgt cttggattgt gctatggcgc gaacacattt tttcacgccg
     1861 ccaaagcata tatcgagggt tatcatactg tgccaaaggg tcggtgccgc caggtcgtga
     1921 ccggcatggc atggctgttt ttcgtgagct ggggtatgtt cccaattctc ttcattttgg
     1981 ggcccgaagg ttttggcgtc ctgagcgtct atggctccac cgtaggtcac acgattattg
     2041 atctgatgag taaaaattgt tgggggttgt tgggacacta cctgcgcgtc ctgatccacg
     2101 agcacatatt gattcacgga gatatccgca aaaccaccaa actgaacatc ggcggaacgg
     2161 agatcgaggt cgagactctc gtcgaagacg aagccgaggc cggagccgtg ccagcggccg
     2221 ccgtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     2281 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     2341 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     2401 tcgtgaccac cttcggctac ggcctgcagt gcttcgcccg ctaccccgac cacatgaagc
     2461 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     2521 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     2581 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     2641 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
     2701 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     2761 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     2821 acctgagcta ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     2881 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaac
     2941 atatgctcta gccaattgcc ggtcatcatc accatcacca ttgagtttaa ttcgatatca
     3001 agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     3061 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     3121 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     3181 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     3241 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     3301 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     3361 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     3421 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     3481 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     3541 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     3601 atcgataccg agcgctgctc gagagatcta cgggtggcat ccctgtgacc cctccccagt
     3661 gcctctcctg gccctggaag ttgccactcc agtgcccacc agccttgtcc taataaaatt
     3721 aagttgcatc attttgtctg actaggtgtc cttctataat attatggggt ggaggggggt
     3781 ggtatggagc aaggggcaag ttgggaagac aacctgtagg gcctgcgggg tctattggga
     3841 accaagctgg agtgcagtgg cacaatcttg gctcactgca atctccgcct cctgggttca
     3901 agcgattctc ctgcctcagc ctcccgagtt gttgggattc caggcatgca tgaccaggct
     3961 cagctaattt ttgttttttt ggtagagacg gggtttcacc atattggcca ggctggtctc
     4021 caactcctaa tctcaggtga tctacccacc ttggcctccc aaattgctgg gattacaggc
     4081 gtgaaccact gctcccttcc ctgtccttct gattttgtag gtaaccacgt gcggaccgag
     4141 cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc
     4201 actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg
     4261 agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat
     4321 ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg
     4381 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     4441 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     4501 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     4561 accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     4621 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     4681 gaacaacact caaccctatc tcgggctatt cttttgattt ataagggatt ttgccgattt
     4741 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     4801 tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     4861 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     4921 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     4981 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
     5041 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     5101 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     5161 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     5221 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     5281 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     5341 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     5401 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     5461 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     5521 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     5581 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     5641 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     5701 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     5761 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     5821 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     5881 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     5941 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     6001 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     6061 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     6121 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     6181 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     6241 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     6301 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     6361 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     6421 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     6481 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     6541 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     6601 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     6661 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     6721 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     6781 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     6841 cctttttacg gttcctggcc ttttgctggc cttttgctca c
//
