LOCUS       Exported                6611 bp ds-DNA     circular SYN 14-JUL-2018
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6611)
  AUTHORS   Hochgeschwender
  TITLE     Direct Submission
  JOURNAL   Exported Jul 22, 2018 from SnapGene 4.1.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6611
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..130
                     /label=AAV2 ITR
                     /note="color: #ffcc99"
     promoter        181..628
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             644..1198
                     /codon_start=1
                     /product="enhanced variant of secreted Gaussia luciferase"
                     /label=Gaussia luciferase
                     /note="human codon-optimized"
                     /note="This feature has 2 segments:
                        1:  644 ..  694 / #ff0000 / signal sequence
                        2:  695 .. 1198 / #00ccff / sbGLuc"
                     /translation="MGVKVLFALICIAVAEAKPTENNEDFNIVAVASNFATTDLDADRG
                     KLPGKKLPLEVLKELEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESA
                     QGGIGEAIVDIPEIPGFKDLEPLEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQ
                     RCATFASKIQGQVDKIKGAGGD"
     CDS             1199..1243
                     /codon_start=1
                     /label=15 aa Linker
                     /note="color: #993300"
                     /translation="GDPLVQCGGIAGSAT"
     CDS             1244..2200
                     /codon_start=1
                     /label=CheRiff
                     /note="color: #870087"
                     /translation="MGGAPAPDAHSAPPGNDSAGGSEYHAPAGYQVNPPYHPVHGYEEQ
                     CSSIYIYYGALWEQETARGFQWFAVFLSALFLAFYGWHAYKASVGWEEVYVCSVELIKV
                     ILEIYFEFTSPAMLFLYGGNITPWLRYAEWLLTCPVILIHLSNITGLSEAYNKRTMALL
                     VSDLGTICMGVTAALATGWVKWLFYCIGLVYGTQTFYNAGIIYVESYYIMPAGGCKKLV
                     LAMTAVYYSSWLMFPGLFIFGPEGMHTLSVAGSTIGHTIADLLSKNIWGLLGHFLRIKI
                     HEHIIMYGDIRRPVSSQFLGRKVDVLAFVTEEDKVAAA"
     CDS             2201..2260
                     /codon_start=1
                     /label=TS
                     /note="color: #ff6600"
                     /translation="KSRITSEGEYIPLDQIDINV"
     CDS             2261..2974
                     /codon_start=1
                     /label=EGFP
                     /note="color: #00ff00"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     misc_feature    3002..3590
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     CDS             complement(3473..3484)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 3472"
                     /translation="IEGR"
     polyA_signal    3638..3845
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     polyA_signal    3638..3845
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     repeat_region   3874..4014
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     rep_origin      4089..4544
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     rep_origin      4089..4544
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        4826..4930
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             4931..5791
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 4931 .. 4999 / #ccffcc / signal sequence
                        2: 5000 .. 5791 / #ccffcc
                     Cleavage site after base 4999"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5962..6550
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgttgtaca gtgtctagac tgcagagggc cctgcgtatg
      181 agtgcaagtg ggttttagga ccaggatgag gcggggtggg ggtgcctacc tgacgaccga
      241 ccccgaccca ctggacaagc acccaacccc cattccccaa attgcgcatc ccctatcaga
      301 gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt cagcaccgcg
      361 gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc
      421 gctgacgtca ctcgccggtc ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg
      481 cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg
      541 cgaccatctg cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg
      601 aggagtcgtg tcgtgcctga gagcgcagtc gagaaggatc cacatgggag tcaaagttct
      661 gtttgccctg atctgcatcg ctgtggccga ggccaagccc accgagaaca acgaagactt
      721 caacatcgtg gccgtggcca gcaacttcgc gaccacggat ctcgatgctg accgcgggaa
      781 gttgcccggc aagaagctgc cgctggaggt gctcaaagag ctggaagcca atgcccggaa
      841 agctggctgc accaggggct gtctgatctg cctgtcccac atcaagtgca cgcccaagat
      901 gaagaagttc atcccaggac gctgccacac ctacgaaggc gacaaagagt ccgcacaggg
      961 cggcataggc gaggcgatcg tcgacattcc tgagattcct gggttcaagg acttggagcc
     1021 cctggagcag ttcatcgcac aggtcgatct gtgtgtggac tgcacaactg gctgcctcaa
     1081 agggcttgcc aacgtgcagt gttctgacct gctcaagaag tggctgccgc aacgctgtgc
     1141 gacctttgcc agcaagatcc agggccaggt ggacaagatc aagggggccg gtggtgacgg
     1201 cgacccacta gtccagtgtg gtggaattgc cggttccgcc actatgggcg gagctcctgc
     1261 tccagacgct cacagcgccc cacctggaaa cgattctgcc ggaggcagtg agtaccatgc
     1321 cccagctgga tatcaagtga atccacccta ccaccccgtg catgggtatg aggaacagtg
     1381 cagctccatc tacatctact atggggccct gtgggagcag gaaacagcta ggggcttcca
     1441 gtggtttgcc gtgttcctgt ctgccctgtt tctggctttc tacggctggc acgcctataa
     1501 ggccagcgtg ggatgggagg aagtgtacgt gtgctccgtg gagctgatca aagtgattct
     1561 ggagatctat ttcgagttca ccagtcctgc tatgctgttc ctgtacggag ggaacattac
     1621 cccatggctg agatatgccg aatggctgct gacatgtccc gtgatcctga ttcatctgtc
     1681 taacatcacc ggcctgagtg aggcatacaa taagcggaca atggctctgc tggtgtccga
     1741 cctgggaact atttgcatgg gagtgacagc cgctctggcc actgggtggg tgaagtggct
     1801 gttttactgt atcggcctgg tgtatggaac ccagacattc tacaacgctg gaatcatcta
     1861 cgtggagtct tactatatca tgcctgccgg cggctgtaag aaactggtgc tggccatgac
     1921 tgccgtgtac tattctagtt ggctgatgtt tcccggcctg ttcatctttg ggcctgaagg
     1981 catgcacacc ctgagcgtgg ctgggtccac tattggccat accatcgccg acctgctgtc
     2041 caagaatatt tggggactgc tggggcactt cctgcggatc aaaattcacg agcatatcat
     2101 tatgtacggc gatatcagga gaccagtgag ctcccagttt ctgggacgca aggtggacgt
     2161 gctggccttc gtgacagagg aagataaagt ggcggccgcc aagagcagga tcaccagcga
     2221 gggcgagtac atccccctgg accagatcga catcaacgtg gtgagcaagg gcgaggagct
     2281 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     2341 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     2401 ttgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     2461 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     2521 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     2581 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     2641 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     2701 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     2761 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     2821 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     2881 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     2941 cgggatcact ctcggcatgg acgagctgta caagtaagaa ttcgatatca agcttatcga
     3001 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     3061 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     3121 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     3181 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     3241 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     3301 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     3361 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     3421 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     3481 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     3541 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg
     3601 agcgctgctc gagctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca
     3661 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     3721 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     3781 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     3841 ggggaggtaa ccacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca
     3901 ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc
     3961 cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc
     4021 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca
     4081 accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     4141 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     4201 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     4261 ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg
     4321 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     4381 taatagtgga ctcttgttcc aaactggaac aacactcaat cctatctcgg gctattcttt
     4441 tgatttataa gggattttgc cgatttcggt ctattggtta aaaaatgagc tgatttaaca
     4501 aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag
     4561 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     4621 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     4681 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
     4741 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     4801 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     4861 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     4921 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     4981 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     5041 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     5101 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     5161 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     5221 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     5281 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     5341 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     5401 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     5461 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     5521 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     5581 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     5641 gcgtggaagc cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     5701 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     5761 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     5821 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     5881 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     5941 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     6001 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     6061 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     6121 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     6181 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     6241 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     6301 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     6361 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     6421 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     6481 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     6541 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     6601 tgctcacatg t
//
