LOCUS pcDNA5-FRT-TO-FL 7826 bp DNA circular 12-JUL-2018 FEATURES Location/Qualifiers enhancer 235..614 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" promoter 615..818 /note="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" protein_bind 820..838 /gene="tetO" /bound_moiety="tetracycline repressor TetR" /note="tet operator" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 841..859 /gene="tetO" /bound_moiety="tetracycline repressor TetR" /note="tet operator" /note="bacterial operator O2 for the tetR and tetA genes" CDS 993..1016 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /note="FLAG" /translation="DYKDDDDK" polyA_signal 3784..4008 /note="bGH poly(A) signal" /note="bovine growth hormone polyadenylation signal" protein_bind 4292..4339 /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="FRT" /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." CDS 4347..5369 /codon_start=1 /product="hygromycin B phosphotransferase" /note="HygR" /note="confers resistance to hygromycin" /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGDFSESLTYCISRRAQGVTLQDL PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYH WQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSE AMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQS LVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPST RPRAKE" polyA_signal 5499..5620 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" primer_bind complement(5669..5685) /note="M13 rev" /note="common sequencing primer, one of multiple similar variants" protein_bind 5693..5709 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(5717..5747) /note="lac promoter" /note="promoter for the E. coli lac operon" protein_bind 5762..5783 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding site" /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(6071..6659) /dnas_title="ori" /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(6830..7690) /dnas_title="AmpR" /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" promoter complement(7691..7795) /dnas_title="AmpR promoter" /gene="bla" /note="AmpR promoter" regulatory 820..859 /dnas_title="TetR operator" /regulatory_class="transcriptional_cis_regulatory_region" /note="Tet operator, derived from Tn10" /note="TetR repressor binding site" /note="Tetracycline operator (2X TetO2) sequence" misc_feature 869..893 /dnas_title="LNCX primer" /label=LNCX primer CDS 1041..3749 /dnas_title="SHLD2 human" /note="SHLD2 human" /translation="SGGSQVHIFWGAPIAPLKITVSEDTASLMSVADPWKKIQLLYSQ HSLYLKDEKQHKNLENYKVPESIGSPDLSGHFLANCMNRHVHVKDDFVRSVSETQNIE SQKIHSSRLSDITSSNMQICGFKSTVPHFTEEEKYQKLLSENKIRDEQPKHQPDICGK NFNTNLFQLGHKCAAVLDLVCSTEKINIGPEVVQRECVPTEYHEIQNQCLGLFSSNAV DKSRSEAAVRKVSDLKISTDTEFLSIITSSQVAFLAQKKDKRRSPVNKGNVNMETEPK ASYGEIRIPEENSIQLDGFTEAYESGQNQAYSLELFSPVCPKTENSRIHINSDKGLEE HTGSQELFSSEDELPPNEIRIELCSSGILCSQLNTFHKSAIKRSCTSEDKVGQSEALS RVLQVAKKMKLISNGGDSAVEMDRRNVSEFKSIKKTSLIKNCDSKSQKYNCLVMVLSP CHVKEINIKFGPNSGSKVPLATVTVIDQSETKKKVFLWRTAAFWAFTVFLGDIILLTD VVIHEDQWIGETVLQSTFSSQLLNLGSYSSIQPEEYSSVVSEVVLQDLLAYVSSKHSY LRDLPPRQPQRVNSIDFVELEHLQPDVLVHAVLRVVDFTILTEAVYSYRGQKQKKVML TVEQAQDQHYALVLWGPGAAWYPQLQRKKGYIWEFKYLFVQCNYTLENLELHTTPWSS CECLFDDDIRAITFKAKFQKSAPSFVKISDLATHLEDKCSGVVLIKAQISELAFPITA SQKIALNAHSSLKSIFSSLPNIVYTGCAKCGLELETDENRIYKQCFSCLPFTMKKIYY RPALMTAIDGRHDVCIRVESKLIEKILLNISADCLNRVIVPSSEITYGMVVADLFHSL LAVSAEPCVLKIQSLFVLDENSYPLQQDFSLLDFYPDIVKHGANARL" regulatory 3784..3991 /dnas_title="BGH polyA signal" /regulatory_class="polyA_signal_sequence" /note="BGH polyadenylation sequence" misc_recomb 4292..4339 /dnas_title="FRT" /note="FRT" /note="recombination site for the flippase recombinase" terminator 5502..5621 /dnas_title="SV40_PA_terminator" /label=SV40_PA_terminator /translation="LFIAAYNGYK.SNSITNFTNKAFFSLHSSCGLSKLINVSY" regulatory 5555..5629 /dnas_title="SV40 early polyA signal" /regulatory_class="polyA_signal_sequence" /note="SV40 early polyadenylation signal" /note="the early polyadenylation signal (EPA) and the late polyadenylation signal (LPA) are on opposite strands of the same region" misc_feature 5590..5609 /dnas_title="EBV_rev_primer" /label=EBV_rev_primer /translation="VVCPNS" CDS complement(5659..5673) /dnas_title="lacZa part A" /gene="lacZ alpha" /codon_start=1 /transl_table=11 /product="Beta-galactosidase alpha peptide" /translation="MTMIT" promoter complement(5665..5683) /dnas_title="M13_reverse_primer" /label=M13_reverse_primer /translation="GNSYDH" misc_feature complement(5682..5704) /dnas_title="M13_pUC_rev_primer" /label=M13_pUC_rev_primer /translation="SG.QFHT" regulatory complement(5691..5711) /dnas_title="lac operator" /regulatory_class="transcriptional_cis_regulatory_region" /note="Lac operator" /note="Lac repressor binding site" regulatory complement(5717..5747) /dnas_title="lac promoter" /regulatory_class="promoter" /note="lac promoter" regulatory complement(5758..5782) /dnas_title="CAP binding site" /regulatory_class="transcriptional_cis_regulatory_region" /note="CAP binding site" regulatory complement(7725..7760) /dnas_title="AmpR promoter" /regulatory_class="promoter" /note="natural promoter for the beta-lactamase gene" /note="includes -35, -10, and transcription start site" CDS 990..3749 /note="FLAG-SHLD2" /translation="MDYKDDDDKAASLNGAPSGGSQVHIFWGAPIAPLKITVSEDTAS LMSVADPWKKIQLLYSQHSLYLKDEKQHKNLENYKVPESIGSPDLSGHFLANCMNRHV HVKDDFVRSVSETQNIESQKIHSSRLSDITSSNMQICGFKSTVPHFTEEEKYQKLLSE NKIRDEQPKHQPDICGKNFNTNLFQLGHKCAAVLDLVCSTEKINIGPEVVQRECVPTE YHEIQNQCLGLFSSNAVDKSRSEAAVRKVSDLKISTDTEFLSIITSSQVAFLAQKKDK RRSPVNKGNVNMETEPKASYGEIRIPEENSIQLDGFTEAYESGQNQAYSLELFSPVCP KTENSRIHINSDKGLEEHTGSQELFSSEDELPPNEIRIELCSSGILCSQLNTFHKSAI KRSCTSEDKVGQSEALSRVLQVAKKMKLISNGGDSAVEMDRRNVSEFKSIKKTSLIKN CDSKSQKYNCLVMVLSPCHVKEINIKFGPNSGSKVPLATVTVIDQSETKKKVFLWRTA AFWAFTVFLGDIILLTDVVIHEDQWIGETVLQSTFSSQLLNLGSYSSIQPEEYSSVVS EVVLQDLLAYVSSKHSYLRDLPPRQPQRVNSIDFVELEHLQPDVLVHAVLRVVDFTIL TEAVYSYRGQKQKKVMLTVEQAQDQHYALVLWGPGAAWYPQLQRKKGYIWEFKYLFVQ CNYTLENLELHTTPWSSCECLFDDDIRAITFKAKFQKSAPSFVKISDLATHLEDKCSG VVLIKAQISELAFPITASQKIALNAHSSLKSIFSSLPNIVYTGCAKCGLELETDENRI YKQCFSCLPFTMKKIYYRPALMTAIDGRHDVCIRVESKLIEKILLNISADCLNRVIVP SSEITYGMVVADLFHSLLAVSAEPCVLKIQSLFVLDENSYPLQQDFSLLDFYPDIVKH GANARL" source 1..7826 /dnas_title="pcDNA5-FRT-TO-FLAG-SHLD2 for Addgene" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc 841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga 901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga 961 ctctagcgtt taaacttaag cttggtacca tggactacaa agacgatgac gataaagccg 1021 ccagtttaaa cggcgcgcca agtggaggat ctcaagtcca cattttttgg ggtgctccaa 1081 ttgctccact gaaaatcaca gtatcagaag acacagcttc tttaatgtct gttgctgacc 1141 cctggaaaaa aattcagctt ttatacagtc aacattcttt atatctgaag gatgaaaaac 1201 agcacaaaaa tcttgaaaac tataaagtcc cagaatctat tggttctcca gatcttagtg 1261 gtcatttctt agcaaactgt atgaatagac atgttcatgt gaaagatgac tttgtacgtt 1321 ctgtttctga aacacagaat atagaatccc agaagattca ctcctctaga ctgagtgata 1381 taactagctc taatatgcaa atatgtggat ttaaaagcac agttccgcat ttcaccgaag 1441 aagaaaagta tcaaaagctt ctcagtgaaa ataaaattag agatgaacag cctaaacatc 1501 agccagatat atgtggtaag aactttaaca caaatttgtt tcagttgggc cataaatgtg 1561 cagctgtgtt ggatttggtt tgtagtactg aaaaaattaa tatagggcct gaagtggtac 1621 aaagagagtg tgtgccaaca gaatatcatg aaatacaaaa ccagtgtttg ggattatttt 1681 cctcgaacgc agtagataag tcaaggtctg aagcagcagt taggaaggtc tcagacctta 1741 aaatatcaac tgatacagaa tttctcagta taattacctc cagccaggtt gcttttttag 1801 ctcaaaagaa agataaaagg cggagtcctg taaataaagg gaatgtaaac atggagactg 1861 aaccaaaggc aagttacggg gagataagaa tacctgaaga gaattcgatt cagcttgatg 1921 gttttacaga agcatatgaa agtggacaaa accaagcata ttcccttgaa ctttttagtc 1981 ctgtttgtcc taaaacagaa aatagccgca ttcacataaa ctctgataaa ggtcttgaag 2041 aacatacagg atctcaagaa cttttcagtt ctgaagatga actgccacca aatgagatac 2101 gtattgagtt gtgtagctca ggaatactgt gttcccaact aaataccttc cacaaaagtg 2161 ctattaaaag aagctgtacc tctgaagata aagtgggcca gtctgaagct ctatctagag 2221 tccttcaagt agctaagaaa atgaagttga tttctaatgg aggagattct gctgtagaaa 2281 tggatcggag aaatgtgtct gaatttaaga gtattaaaaa aacatcatta ataaaaaact 2341 gtgattctaa aagccagaag tataattgtt tagtcatggt gctatctcca tgccatgtga 2401 aggaaataaa cataaaattc ggaccaaatt ctggctctaa agtgccttta gcaacagtta 2461 cagtaattga tcaatcagaa actaagaaga aggtttttct gtggaggact gcagcatttt 2521 gggcatttac agtgtttctt ggagatataa ttttactcac agatgttgtt attcatgagg 2581 accaatggat tggcgagaca gtactacaat caacatttag cagtcagtta ttaaatcttg 2641 ggagttattc atctattcag cctgaagaat attccagtgt agttagtgaa gttgtacttc 2701 aagacttact ggcatatgtg tcctcaaaac attcctacct cagagatctt cctccgaggc 2761 agcctcagag ggtgaacagt atagactttg tagaattgga gcaccttcaa cctgatgtat 2821 tagtccacgc agtactaaga gttgttgatt tcactatact gacagaggca gtatacagtt 2881 atagaggaca gaagcagaaa aaagttatgt taacagtgga acaggcccaa gatcaacatt 2941 atgcgcttgt attatggggt cctggagcag cctggtaccc tcaacttcaa aggaaaaaag 3001 gttatatttg ggaatttaaa tatctttttg ttcagtgcaa ttacacacta gaaaacctag 3061 aattgcatac aacgccttgg tcatcctgtg agtgcttgtt tgatgatgat ataagggcaa 3121 ttacatttaa agcaaaattt caaaaaagtg caccctcctt tgtgaagata tcagacttag 3181 caacccacct agaggataag tgttcaggag tggttctgat taaagcccag atttcagagc 3241 tggcatttcc tattacagca tctcagaaga tagcgctaaa tgctcacagt tctctgaaga 3301 gtattttttc ttctcttccc aacatcgtat atactggttg tgcaaaatgt ggattggaac 3361 tagaaacaga tgagaacagg atctacaaac aatgttttag ctgcttgcca tttactatga 3421 agaaaatata ttataggcca gcgttaatga ctgccattga tggaagacat gatgtttgta 3481 tccgtgtaga atcaaagctg atagagaaga ttcttctcaa catttctgca gactgcctca 3541 acagagtgat agttccttcc tcagagatca cctatgggat ggtcgtggca gacctgttcc 3601 actccttgtt ggcagtcagc gcagaacctt gtgtattaaa gattcagagc ctttttgtgt 3661 tagatgaaaa cagctatcca ttacaacaag atttctccct cctggatttt tatcctgaca 3721 ttgtaaagca tggagccaat gcccgtctct gagggcccgt ttaaacccgc tgatcagcct 3781 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga 3841 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt 3901 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg 3961 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg 4021 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg 4081 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg 4141 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc 4201 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa 4261 aacttgatta gggtgatggt tcacgtacct agaagttcct attccgaagt tcctattctc 4321 tagaaagtat aggaacttcc ttggccaaaa agcctgaact caccgcgacg tctgtcgaga 4381 agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag 4441 aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct 4501 gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc 4561 cgattccgga agtgcttgac attggggatt tcagcgagag cctgacctat tgcatctccc 4621 gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc 4681 agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt 4741 tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg 4801 cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt 4861 ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc 4921 acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag 4981 cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct 5041 tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc 5101 atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc 5161 aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat 5221 gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa 5281 gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc 5341 ccagcactcg tccgagggca aaggaatagc acgtactacg agatttcgat tccaccgccg 5401 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc 5461 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata 5521 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc 5581 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga 5641 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc 5701 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct 5761 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 5821 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 5881 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 5941 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 6001 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 6061 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 6121 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 6181 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 6241 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 6301 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 6361 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 6421 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 6481 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga 6541 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 6601 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 6661 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 6721 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 6781 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 6841 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 6901 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 6961 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 7021 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 7081 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 7141 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 7201 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 7261 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 7321 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 7381 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 7441 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 7501 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 7561 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 7621 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 7681 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 7741 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 7801 ttccccgaaa agtgccacct gacgtc //