LOCUS       Exported               11146 bp ds-DNA     circular SYN 20-NOV-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11146)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 20, 2017 from SnapGene Viewer 4.1.0
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     misc_feature    41..158
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        212..526
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of
                     seven tet operator sequences followed by the minimal CMV
                     promoter"
     protein_bind    220..238
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    256..274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    291..309
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    327..345
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    363..381
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    398..416
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    434..452
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    547..571
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             580..1296
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK"
     protein_bind    complement(4019..4043)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4061..4078
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        4088..4598
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     CDS             4608..5204
                     /dnas_title="PuroR"
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
                     ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     CDS             5205..5258
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5259..6005
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to
                     the Tet-responsive element and stimulates transcription
                     only in the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLD
                     MLPADALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(6463..6474)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6679..6912
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR)
                     from HIV-1"
     polyA_signal    6984..7105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      7145..7280
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7280..7351
                     /dnas_title="AmpR promoter"
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7352..8212
                     /dnas_title="AmpR"
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     rep_origin      8383..8971
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    9259..9280
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the
                     presence of cAMP."
     promoter        9295..9325
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9333..9349
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9357..9373
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        9394..9412
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9440..9666
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9667..9847
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9894..10019
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    10512..10745
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     misc_feature    45..60
                     /dnas_title="cPPT"
                     /label=cPPT
     misc_feature    497..516
                     /dnas_title="pCEP_fwd_primer"
                     /label=pCEP_fwd_primer
     misc_feature    499..523
                     /dnas_title="LNCX primer"
                     /label=LNCX primer
     misc_recomb     547..571
                     /dnas_title="attB1"
                     /note="attB1"
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    547..571
                     /dnas_title="final attB1 site"
                     /note="final attB1 site"
                     /note="in expression clone"
     CDS             1306..4014
                     /dnas_title="SHLD2 (m1 mutant)"
                     /note="SHLD2 (m1 mutant)"
                     /translation="SGGSQVHIFWGAPIAPLKITVSEDTASLMSVADPWKKIQLLYSQ
                     HSLYLKDEKQHKNLENYKVPESIGSPDLSGHFLANCMNRHVHVKDDFVRSVSETQNIE
                     SQKIHSSRLSDITSSNMQICGFKSTVPHFTEEEKYQKLLSENKIRDEQPKHQPDICGK
                     NFNTNLFQLGHKCAAVLDLVCSTEKINIGPEVVQRECVPTEYHEIQNQCLGLFSSNAV
                     DKSRSEAAVRKVSDLKISTDTEFLSIITSSQVAFLAQKKDKRRSPVNKGNVNMETEPK
                     ASYGEIRIPEENSIQLDGFTEAYESGQNQAYSLELFSPVCPKTENSRIHINSDKGLEE
                     HTGSQELFSSEDELPPNEIRIELCSSGILCSQLNTFHKSAIKRSCTSEDKVGQSEALS
                     RVLQVAKKMKLISNGGDSAVEMDRRNVSEFKSIKKTSLIKNCDSKSQKYNCLVMVLSP
                     CHVKEINIKFGPNSGSKVPLATVTVIDQSETKKKVFLARTAAAAAFTVFLGDIILLTD
                     VVIHEDQWIGETVLQSTFSSQLLNLGSYSSIQPEEYSSVVSEVVLQDLLAYVSSKHSY
                     LRDLPPRQPQRVNSIDFVELEHLQPDVLVHAVLRVVDFTILTEAVYSYRGQKQKKVML
                     TVEQAQDQHYALVLWGPGAAWYPQLQRKKGYIWEFKYLFVQCNYTLENLELHTTPWSS
                     CECLFDDDIRAITFKAKFQKSAPSFVKISDLATHLEDKCSGVVLIKAQISELAFPITA
                     SQKIALNAHSSLKSIFSSLPNIVYTGCAKCGLELETDENRIYKQCFSCLPFTMKKIYY
                     RPALMTAIDGRHDVCIRVESKLIEKILLNISADCLNRVIVPSSEITYGMVVADLFHSL
                     LAVSAEPCVLKIQSLFVLDENSYPLQQDFSLLDFYPDIVKHGANARL"
                     /note="FM9 = WFW 489, 493, 494 AAA  (manuscript = mutant
                     m1)"
     CDS             2767..2787
                     /dnas_title="m1 mutation"
                     /note="WFW-->AAA   (489, 494, 495)"
                     /translation="ARTAAAA"
                     /note="m1 mutation "
                     /note="W489A/F494A/W495A"
     misc_feature    4019..4043
                     /dnas_title="final attB2 site"
                     /note="final attB2 site"
                     /note="in expression clone"
     misc_recomb     complement(4019..4043)
                     /dnas_title="attB2"
                     /note="attB2"
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    complement(4193..4212)
                     /dnas_title="OPGK1b-R primer rks903/DD2288"
                     /note="OPGK1b-R primer rks903/DD2288"
     misc_feature    6042..6569
                     /dnas_title="WPRE"
                     /label=WPRE
                     /translation="LCCSFYAMWIRCFNAFVSCYCFPYGFHFLLLV.ILVAVSL.GVV
                     ARCQATWRGVHCVC.RNPHWLGHCHHLSAPFRDFRFPPPYCHGGTHRRLPCPLLDRGS
                     AVGH.QFRGVVGEADVLSMAARLCCHLDSARDVLLLRPFGPQSSGPSFPRPAAGSAAS
                     SASSPSPSDESDLPLG"
     misc_feature    6662..6677
                     /dnas_title="cPPT"
                     /label=cPPT
     misc_feature    6662..6683
                     /dnas_title="U3PPT"
                     /label=U3PPT
                     /translation="KRKGGTG"
     misc_feature    6679..6731
                     /dnas_title="delta_U3"
                     /label=delta_U3
                     /translation="WKG.FTPNEDKICFLLV"
     repeat_region   6679..6913
                     /dnas_title="HIV-1 3' LTR"
                     /rpt_type=long_terminal_repeat
                     /note="HIV-1 deltaU3 3' LTR"
     repeat_region   6732..6912
                     /dnas_title="HIV-1 5' LTR"
                     /rpt_type=long_terminal_repeat
                     /note="HIV-1 5' LTR"
     regulatory      7040..7114
                     /dnas_title="SV40 early polyA signal"
                     /regulatory_class="polyA_signal_sequence"
                     /note="SV40 early polyadenylation signal"
                     /note="the early polyadenylation signal (EPA) and the
                     late polyadenylation signal (LPA) are on opposite strands
                     of the same region"
     misc_feature    7075..7094
                     /dnas_title="EBV_rev_primer"
                     /label=EBV_rev_primer
                     /translation="VVCPNS"
     rep_origin      7124..7222
                     /dnas_title="SV40_origin"
                     /label=SV40_origin
                     /translation="IPPLTPPIPPLTPPSSAHSPPHG.LIFFIYAEA"
     rep_origin      7197..7260
                     /dnas_title="SV40 ori"
                     /note="SV40 origin of replication"
                     /note="this core origin is both necessary and sufficient
                     for replication"
     misc_feature    7207..7226
                     /dnas_title="SV40pro_F_primer"
                     /label=SV40pro_F_primer
                     /translation="YLCRGR"
     promoter        7282..7310
                     /dnas_title="AmpR_promoter"
                     /label=AmpR_promoter
                     /translation="FKYVSAHET"
     regulatory      7282..7317
                     /dnas_title="AmpR promoter"
                     /regulatory_class="promoter"
                     /note="natural promoter for the beta-lactamase gene"
                     /note="includes -35, -10, and transcription start site"
     protein_bind    complement(9259..9280)
                     /dnas_title="E. coli catabolite activator protein"
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the
                     presence of cAMP."
     regulatory      9260..9284
                     /dnas_title="CAP binding site"
                     /regulatory_class="transcriptional_cis_regulatory_region"
                     /note="CAP binding site"
     regulatory      9295..9325
                     /dnas_title="lac promoter"
                     /regulatory_class="promoter"
                     /note="lac promoter"
     regulatory      9331..9351
                     /dnas_title="lac operator"
                     /regulatory_class="transcriptional_cis_regulatory_region"
                     /note="Lac operator"
                     /note="Lac repressor binding site"
     protein_bind    complement(9333..9349)
                     /dnas_title="lac repressor encoded by lacI"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    9338..9360
                     /dnas_title="M13_pUC_rev_primer"
                     /label=M13_pUC_rev_primer
                     /translation="SG.QFHT"
     promoter        9359..9377
                     /dnas_title="M13_reverse_primer"
                     /label=M13_reverse_primer
                     /translation="GNSYDH"
     CDS             9369..9383
                     /dnas_title="lacZa part A"
                     /gene="lacZ alpha"
                     /codon_start=1
                     /transl_table=11
                     /product="Beta-galactosidase alpha peptide"
                     /translation="MTMIT"
     regulatory      9394..9411
                     /dnas_title="T3 promoter"
                     /regulatory_class="promoter"
                     /note="T3 promoter"
                     /note="bacteriophage T3 RNA polymerase promoter"
     regulatory      9438..9666
                     /dnas_title="RSV promoter"
                     /regulatory_class="promoter"
                     /note="RSV promoter"
     regulatory      9438..9847
                     /dnas_title="RSV LTR promoter"
                     /regulatory_class="promoter"
                     /note="RSV/5' LTR hybrid promoter"
                     /note="hybrid of the Rous Sarcoma virus promoter and the
                     HIV 5' LTR"
     misc_feature    9667..9847
                     /dnas_title="HIV-1_5_LTR"
                     /label=HIV-1_5_LTR
                     /translation="GSLWLDQI.AWELSG.LGNPLLKPQ.SLP.VLQVVCARLLCDSG
                     N.RSLRPF.SVWKISS"
     repeat_region   9667..9847
                     /dnas_title="HIV-1 5' LTR"
                     /rpt_type=long_terminal_repeat
                     /note="HIV-1 5' LTR"
     misc_feature    9849..9866
                     /dnas_title="HIV-1 PBS"
                     /note="primer binding site (PBS)"
                     /note="complementary to the 3%E2%80^2-end of tRNA-Lys3"
     misc_feature    9902..10021
                     /dnas_title="HIV-1 psi"
                     /note="HIV-1 psi (psi) packaging signal"
                     /note="contains SL1, SL2, SL3, and SL4 stem-loops"
     misc_feature    9958..10002
                     /dnas_title="HIV-1_psi_pack"
                     /label=HIV-1_psi_pack
                     /translation=".VRQKF.LAEARRRE"
     CDS             580..4014
                     /note="eGFP-SHLD2 (m1)"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKGAPSGGSQVHIFWGAPIAPLKITVSEDTASLMSVADP
                     WKKIQLLYSQHSLYLKDEKQHKNLENYKVPESIGSPDLSGHFLANCMNRHVHVKDDFV
                     RSVSETQNIESQKIHSSRLSDITSSNMQICGFKSTVPHFTEEEKYQKLLSENKIRDEQ
                     PKHQPDICGKNFNTNLFQLGHKCAAVLDLVCSTEKINIGPEVVQRECVPTEYHEIQNQ
                     CLGLFSSNAVDKSRSEAAVRKVSDLKISTDTEFLSIITSSQVAFLAQKKDKRRSPVNK
                     GNVNMETEPKASYGEIRIPEENSIQLDGFTEAYESGQNQAYSLELFSPVCPKTENSRI
                     HINSDKGLEEHTGSQELFSSEDELPPNEIRIELCSSGILCSQLNTFHKSAIKRSCTSE
                     DKVGQSEALSRVLQVAKKMKLISNGGDSAVEMDRRNVSEFKSIKKTSLIKNCDSKSQK
                     YNCLVMVLSPCHVKEINIKFGPNSGSKVPLATVTVIDQSETKKKVFLARTAAAAAFTV
                     FLGDIILLTDVVIHEDQWIGETVLQSTFSSQLLNLGSYSSIQPEEYSSVVSEVVLQDL
                     LAYVSSKHSYLRDLPPRQPQRVNSIDFVELEHLQPDVLVHAVLRVVDFTILTEAVYSY
                     RGQKQKKVMLTVEQAQDQHYALVLWGPGAAWYPQLQRKKGYIWEFKYLFVQCNYTLEN
                     LELHTTPWSSCECLFDDDIRAITFKAKFQKSAPSFVKISDLATHLEDKCSGVVLIKAQ
                     ISELAFPITASQKIALNAHSSLKSIFSSLPNIVYTGCAKCGLELETDENRIYKQCFSC
                     LPFTMKKIYYRPALMTAIDGRHDVCIRVESKLIEKILLNISADCLNRVIVPSSEITYG
                     MVVADLFHSLLAVSAEPCVLKIQSLFVLDENSYPLQQDFSLLDFYPDIVKHGANARL"
     source          1..11146
                     /dnas_title="pCW-eGFP-SHLD2 (m1) for Addgene"
ORIGIN      
        1 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
       61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      181 gagatccact ttggccgcga atcgatatgt cgagtttact ccctatcagt gatagagaac
      241 gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag
      301 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
      361 actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt
      421 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg
      481 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattggcta
      541 gcatcaacaa gtttgtacaa aaaagcaggc tccggtacca tggtgagcaa gggcgaggag
      601 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
      661 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
      721 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
      781 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
      841 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
      901 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
      961 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     1021 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     1081 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     1141 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
     1201 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     1261 gccgggatca ctctcggcat ggacgagctg tacaagggcg cgccaagtgg aggatctcaa
     1321 gtccacattt tttggggtgc tccaattgct ccactgaaaa tcacagtatc agaagacaca
     1381 gcttctttaa tgtctgttgc tgacccctgg aaaaaaattc agcttttata cagtcaacat
     1441 tctttatatc tgaaggatga aaaacagcac aaaaatcttg aaaactataa agtcccagaa
     1501 tctattggtt ctccagatct tagtggtcat ttcttagcaa actgtatgaa tagacatgtt
     1561 catgtgaaag atgactttgt acgttctgtt tctgaaacac agaatataga atcccagaag
     1621 attcactcct ctagactgag tgatataact agctctaata tgcaaatatg tggatttaaa
     1681 agcacagttc cgcatttcac cgaagaagaa aagtatcaaa agcttctcag tgaaaataaa
     1741 attagagatg aacagcctaa acatcagcca gatatatgtg gtaagaactt taacacaaat
     1801 ttgtttcagt tgggccataa atgtgcagct gtgttggatt tggtttgtag tactgaaaaa
     1861 attaatatag ggcctgaagt ggtacaaaga gagtgtgtgc caacagaata tcatgaaata
     1921 caaaaccagt gtttgggatt attttcctcg aacgcagtag ataagtcaag gtctgaagca
     1981 gcagttagga aggtctcaga ccttaaaata tcaactgata cagaatttct cagtataatt
     2041 acctccagcc aggttgcttt tttagctcaa aagaaagata aaaggcggag tcctgtaaat
     2101 aaagggaatg taaacatgga gactgaacca aaggcaagtt acggggagat aagaatacct
     2161 gaagagaatt cgattcagct tgatggtttt acagaagcat atgaaagtgg acaaaaccaa
     2221 gcatattccc ttgaactttt tagtcctgtt tgtcctaaaa cagaaaatag ccgcattcac
     2281 ataaactctg ataaaggtct tgaagaacat acaggatctc aagaactttt cagttctgaa
     2341 gatgaactgc caccaaatga gatacgtatt gagttgtgta gctcaggaat actgtgttcc
     2401 caactaaata ccttccacaa aagtgctatt aaaagaagct gtacctctga agataaagtg
     2461 ggccagtctg aagctctatc tagagtcctt caagtagcta agaaaatgaa gttgatttct
     2521 aatggaggag attctgctgt agaaatggat cggagaaatg tgtctgaatt taagagtatt
     2581 aaaaaaacat cattaataaa aaactgtgat tctaaaagcc agaagtataa ttgtttagtc
     2641 atggtgctat ctccatgcca tgtgaaggaa ataaacataa aattcggacc aaattctggc
     2701 tctaaagtgc ctttagcaac agttacagta attgatcaat cagaaactaa gaagaaggtt
     2761 tttctgGCga ggactgcagc aGCtGCggca tttacagtgt ttcttggaga tataatttta
     2821 ctcacagatg ttgttattca tgaggaccaa tggattggcg agacagtact acaatcaaca
     2881 tttagcagtc agttattaaa tcttgggagt tattcatcta ttcagcctga agaatattcc
     2941 agtgtagtta gtgaagttgt acttcaagac ttactggcat atgtgtcctc aaaacattcc
     3001 tacctcagag atcttcctcc gaggcagcct cagagggtga acagtataga ctttgtagaa
     3061 ttggagcacc ttcaacctga tgtattagtc cacgcagtac taagagttgt tgatttcact
     3121 atactgacag aggcagtata cagttataga ggacagaagc agaaaaaagt tatgttaaca
     3181 gtggaacagg cccaagatca acattatgcg cttgtattat ggggtcctgg agcagcctgg
     3241 taccctcaac ttcaaaggaa aaaaggttat atttgggaat ttaaatatct ttttgttcag
     3301 tgcaattaca cactagaaaa cctagaattg catacaacgc cttggtcatc ctgtgagtgc
     3361 ttgtttgatg atgatataag ggcaattaca tttaaagcaa aatttcaaaa aagtgcaccc
     3421 tcctttgtga agatatcaga cttagcaacc cacctagagg ataagtgttc aggagtggtt
     3481 ctgattaaag cccagatttc agagctggca tttcctatta cagcatctca gaagatagcg
     3541 ctaaatgctc acagttctct gaagagtatt ttttcttctc ttcccaacat cgtatatact
     3601 ggttgtgcaa aatgtggatt ggaactagaa acagatgaga acaggatcta caaacaatgt
     3661 tttagctgct tgccatttac tatgaagaaa atatattata ggccagcgtt aatgactgcc
     3721 attgatggaa gacatgatgt ttgtatccgt gtagaatcaa agctgataga gaagattctt
     3781 ctcaacattt ctgcagactg cctcaacaga gtgatagttc cttcctcaga gatcacctat
     3841 gggatggtcg tggcagacct gttccactcc ttgttggcag tcagcgcaga accttgtgta
     3901 ttaaagattc agagcctttt tgtgttagat gaaaacagct atccattaca acaagatttc
     3961 tccctcctgg atttttatcc tgacattgta aagcatggag ccaatgcccg tctctgagac
     4021 ccagctttct tgtacaaagt ggtttagtaa tgaaccggtc caccaccacc accaccacta
     4081 aggatccggg gttggggttg cgccttttcc aaggcagccc tgggtttgcg cagggacgcg
     4141 gctgctctgg gcgtggttcc gggaaacgca gcggcgccga ccctgggtct cgcacattct
     4201 tcacgtccgt tcgcagcgtc acccggatct tcgccgctac ccttgtgggc cccccggcga
     4261 cgcttcctgc tccgccccta agtcgggaag gttccttgcg gttcgcggcg tgccggacgt
     4321 gacaaacgga agccgcacgt ctcactagta ccctcgcaga cggacagcgc cagggagcaa
     4381 tggcagcgcg ccgaccgcga tgggctgtgg ccaatagcgg ctgctcagca gggcgcgccg
     4441 agagcagcgg ccgggaaggg gcggtgcggg aggcggggtg tggggcggta gtgtgggccc
     4501 tgttcctgcc cgcgcggtgt tccgcattct gcaagcctcc ggagcgcacg tcggcagtcg
     4561 gctccctcgt tgaccgaatc accgacctct ctccccagca attcaccatg accgagtaca
     4621 agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg
     4681 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc
     4741 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
     4801 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
     4861 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
     4921 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
     4981 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
     5041 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
     5101 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
     5161 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgccgaaggt agaggttctc
     5221 tcctcacttg tggtgatgtt gaagaaaacc ctggtccaat gtctagactg gacaagagca
     5281 aagtcataaa cggagctctg gaattactca atggtgtcgg tatcgaaggc ctgacgacaa
     5341 ggaaactcgc tcaaaagctg ggagttgagc agcctaccct gtactggcac gtgaagaaca
     5401 agcgggccct gctcgatgcc ctgccaatcg agatgctgga caggcatcat acccacttct
     5461 gccccctgga aggcgagtca tggcaagact ttctgcggaa caacgccaag tcataccgct
     5521 gtgctctcct ctcacatcgc gacggggcta aagtgcatct cggcacccgc ccaacagaga
     5581 aacagtacga aaccctggaa aatcagctcg cgttcctgtg tcagcaaggc ttctccctgg
     5641 agaacgcact gtacgctctg tccgccgtgg gccactttac actgggctgc gtattggagg
     5701 aacaggagca tcaagtagca aaagaggaaa gagagacacc taccaccgat tctatgcccc
     5761 cacttctgag acaagcaatt gagctgttcg accggcaggg agccgaacct gccttccttt
     5821 tcggcctgga actaatcata tgtggcctgg agaaacagct aaagtgcgaa agcggcgggc
     5881 cgaccgacgc ccttgacgat tttgacttag acatgctccc agccgatgcc cttgacgact
     5941 ttgaccttga tatgctgcct gctgacgctc ttgacgattt tgaccttgac atgctccccg
     6001 ggtaactaag taaggatcga tccaagatat cgtattctta actatgttgc tccttttacg
     6061 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     6121 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     6181 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     6241 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     6301 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     6361 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt
     6421 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     6481 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     6541 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctgtttcgcc tcggcgtccg
     6601 gactagaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     6661 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     6721 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     6781 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     6841 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     6901 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     6961 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
     7021 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     7081 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
     7141 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     7201 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     7261 aggaggcttt tttggaggcc tttcaaatat gtatccgctc atgagacaat aaccctgata
     7321 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     7381 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     7441 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     7501 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     7561 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     7621 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     7681 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     7741 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     7801 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     7861 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     7921 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     7981 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     8041 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     8101 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     8161 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     8221 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     8281 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     8341 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     8401 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     8461 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     8521 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     8581 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     8641 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     8701 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     8761 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     8821 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     8881 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     8941 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     9001 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     9061 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     9121 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     9181 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     9241 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     9301 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     9361 aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa
     9421 gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg caacatggta
     9481 acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg
     9541 gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct gacatggatt
     9601 ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct agctcgatac
     9661 ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     9721 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     9781 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     9841 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc
     9901 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga
     9961 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca
    10021 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa
    10081 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
    10141 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
    10201 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
    10261 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
    10321 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga
    10381 ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt
    10441 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga
    10501 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc
    10561 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag
    10621 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg
    10681 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag
    10741 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat
    10801 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg
    10861 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac
    10921 cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat
    10981 tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc
    11041 ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga
    11101 tattcaccat tatcgtttca gacccacctc ccaaccccga ggggac
//

