LOCUS       pCW-eGFP-SHLD3          9240 bp    DNA     circular     12-JUL-2018
FEATURES             Location/Qualifiers
     source          1..9240
                     /dnas_title="pCW-eGFP-SHLD3 for Addgene"
                     /organism="pCW57.1"
                     /mol_type="other DNA"
     misc_feature    45..60
                     /label=cPPT
     misc_feature    497..516
                     /label=pCEP_fwd_primer
     misc_feature    499..523
                     /label=LNCX primer
     CDS             complement(2123..2703)
                     /label=ORF frame 1
                     /translation="MVNCWGERSVIRSTREPTADVRSGGLQNAEHRAGRNRAHTTAPH
                     PASRTAPSRPLLSARPAEQPLLATAHRGRRAAIAPWRCPSARVLVRRAASVCHVRHAA
                     NRKEPSRLRGGAGSVAGGPTRVAAKIRVTLRTDVKNVRDPGSAPLRFPEPRPEQPRPC
                     ANPGLPWKRRNPNPGSLVVVVVVDRFITKPLCT"
     CDS             2474..4099
                     /label=ORF frame 2
                     /translation="MAARRPRWAVANSGCSAGRAESSGREGAVREAGCGAVVWALFLP
                     ARCSAFCKPPERTSAVGSLVDRITDLSPQQFTMTEYKPTVRLATRDDVPRAVRTLAAA
                     FADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEA
                     GAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSA
                     VVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAE
                     GRGSLLTCGDVEENPGPMSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTL
                     YWHVKNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKV
                     HLGTRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEE
                     RETPTTDSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDF
                     DLDMLPADALDDFDLDMLPADALDDFDLDMLPG"
     gene            2702..3289
                     /dnas_title="Puro"
                     /label=puro (variant)
                     /gene="puro (variant)"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
                     ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRK"
     misc_feature    4136..4663
                     /label=WPRE
                     /translation="LCCSFYAMWIRCFNAFVSCYCFPYGFHFLLLV.ILVAVSL.GVV
                     ARCQATWRGVHCVC.RNPHWLGHCHHLSAPFRDFRFPPPYCHGGTHRRLPCPLLDRGS
                     AVGH.QFRGVVGEADVLSMAARLCCHLDSARDVLLLRPFGPQSSGPSFPRPAAGSAAS
                     SASSPSPSDESDLPLG"
     CDS             4176..4700
                     /label=ORF frame 3
                     /translation="MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVF
                     ADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGK
                     LTSFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRIS
                     LWAASPPVSPRRPD"
     misc_feature    4756..4771
                     /label=cPPT
                     /translation="KRKGG"
     misc_feature    4756..4777
                     /label=U3PPT
                     /translation="KRKGGTG"
     misc_feature    4773..4825
                     /label=delta_U3
                     /translation="WKG.FTPNEDKICFLLV"
     misc_feature    4826..5006
                     /label=HIV-1_5_LTR
                     /translation="GSLWLDQI.AWELSG.LGNPLLKPQ.SLP.VLQVVCARLLCDSG
                     N.RSLRPF.SVWKISS"
     misc_feature    4826..5006
                     /label=truncHIV-1_3_LTR
                     /translation="GSLWLDQI.AWELSG.LGNPLLKPQ.SLP.VLQVVCARLLCDSG
                     N.RSLRPF.SVWKISS"
     terminator      5081..5200
                     /label=SV40_PA_terminator
                     /translation="LFIAAYNGYK.SNSITNFTNKAFFSLHSSCGLSKLINVSY"
     misc_feature    5169..5188
                     /label=EBV_rev_primer
                     /translation="VVCPNS"
     rep_origin      5218..5316
                     /label=SV40_origin
                     /translation="IPPLTPPIPPLTPPSSAHSPPHG.LIFFIYAEA"
     misc_feature    5301..5320
                     /label=SV40pro_F_primer
                     /translation="YLCRGR"
     promoter        5376..5404
                     /label=AmpR_promoter
                     /translation="FKYVSAHET"
     gene            5446..6306
                     /dnas_title="Ampicillin"
                     /label=Ampicillin
                     /gene="Ampicillin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     CDS             5446..6306
                     /label=ORF frame 1
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     rep_origin      6461..7080
                     /label=pBR322_origin
                     /translation="RSKDLLEILFFCA.SAACKQKNHRYQRWFVCRIKSYQLFFRR.L
                     ASAERRYQILFF.CSRS.ATTSRTL.HRLHTSLC.SCYQWLLPVAISRVLPGWTQDDS
                     YRIRRSGRAERGVRAHSPAWSERPTPN.DTYSVSYEKAPRFPKGERRTGIR.AAGSEQ
                     ESARGSFQGETPGIFIVLSGFATSDLSVDFCDARQGGGAYGKTPAT"
     promoter        7389..7418
                     /label=lac_promoter
                     /translation="FTLYASGSYV"
     misc_feature    7432..7454
                     /label=M13_pUC_rev_primer
                     /translation="SG.QFHT"
     promoter        7453..7471
                     /label=M13_reverse_primer
                     /translation="GNSYDH"
     promoter        7488..7507
                     /label=T3_promoter
                     /translation="N.PSLK"
     misc_feature    7761..7941
                     /label=HIV-1_5_LTR
                     /translation="GSLWLDQI.AWELSG.LGNPLLKPQ.SLP.VLQVVCARLLCDSG
                     N.RSLRPF.SVWKISS"
     misc_feature    7761..7941
                     /label=truncHIV-1_3_LTR
                     /translation="GSLWLDQI.AWELSG.LGNPLLKPQ.SLP.VLQVVCARLLCDSG
                     N.RSLRPF.SVWKISS"
     misc_feature    8052..8096
                     /label=HIV-1_psi_pack
                     /translation=".VRQKF.LAEARRRE"
     CDS             410..8484
                     /label=ORF frame 3
                     /translation="IENVCRVYSLSVIENVCRGRRVRWEAYISRARLVNRQIAWRIG.
                     HQQVCTKKQAPVPW.ARARSCSPGWCPSWSSWTAT.TATSSACPARARAMPPTAS.P.
                     SSSAPPASCPCPGPPS.PP.PTACSASAATPTT.SSTTSSSPPCPKATSRSAPSSSRT
                     TATTRPAPR.SSRATPW.TASS.RASTSRRTATSWGTSWSTTTTATTSISWPTSRRTA
                     SR.TSRSATTSRTAACSSPTTTSRTPPSATAPCCCPTTTT.APSPP.AKTPTRSAITW
                     SCWSS.PPPGSLSAWTSCTRARQLQK.YYIIDHVRVIPHNCQKLQKKQFKTFLLVRYQ
                     DLYLGFHMMGPSFHSDLKDHHL.FLKRQLKM.NST.PFQNMMLSHTVMIAQ.IYWSFN
                     LA.KSSI.PGHTH.RNRLILEIWVNNQKRENSTRGDLGVFPFPAIIVLKTFLLCLKNC
                     KIV.RH.IYTHFIEQDGQ.NTLFVTAKLWKTFGQNSIKLLGTMNFHLVMLQFRGI.AK
                     YGCSVILCIVNMWEVFLKED.LLLEKLIYLCINMVLFLVCSSSLEGPFKPADQPRLCL
                     LAPSFLVQSGLVMNRSTTTTTTKDPGLGLRLFQGSPGFAQGRGCSGRGSGKRSGADPG
                     SRTFFTSVRSVTRIFAATLVGPPATLPAPPLSREGSLRFAACRT.QTEAARLTSTLAD
                     GQRQGAMAARRPRWAVANSGCSAGRAESSGREGAVREAGCGAVVWALFLPARCSAFCK
                     PPERTSAVGSLVDRITDLSPQQFTMTEYKPTVRLATRDDVPRAVRTLAAAFADYPATR
                     HTVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIG
                     PRMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEA
                     AERAGVPAFLETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAEGRGSLLTC
                     GDVEENPGPMSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHVKNKR
                     ALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTRPTE
                     KQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTTDS
                     MPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPAD
                     ALDDFDLDMLPADALDDFDLDMLPG.LSKDRSKISYS.LCCSFYAMWIRCFNAFVSCY
                     CFPYGFHFLLLV.ILVAVSL.GVVARCQATWRGVHCVC.RNPHWLGHCHHLSAPFRDF
                     RFPPPYCHGGTHRRLPCPLLDRGSAVGH.QFRGVVGEADVLSMAARLCCHLDSARDVL
                     LLRPFGPQSSGPSFPRPAAGSAASSASSPSPSDESDLPLGRLPACFASASGLEVPLRP
                     MTYKAAVDLSHFLKEKGGLEGLIHSQRRQDLLFACTGSLWLDQI.AWELSG.LGNPLL
                     KPQ.SLP.VLQVVCARLLCDSGN.RSLRPF.SVWKISSSSSSCHLIIQYL.LAKK.IS
                     ESERNLFIAAYNGYK.SNSITNFTNKAFFSLHSSCGLSKLINVSYHVWL.LSRP.LRP
                     SRP.LRPVPPILRPMAD.FFLFMQRPRPPRPLSYSRSSEEAFLEAFQICIRS.DNNPD
                     KCFNNIEKGRV.VFNISVSPLFPFLRHFAFLFLLTQKRW.K.KMLKISWVHEWVTSNW
                     ISTAVRSLRVFAPKNVFQ..ALLKFCYVARYYPVLTPGKSNSVAAYTILRMTWLSTHQ
                     SQKSILRMA.Q.ENYAVLP.P.VITLRPTYF.QRSEDRRS.PLFCTTWGIM.LALIVG
                     NRS.MKPYQTTSVTPRCL.QWQQRCANY.LANYLL.LPGNN..TGWRRIKLQDHFCAR
                     PFRLAGLLLINLEPVSVGLAVSLQHWGQMVSPPVS.LSTRRGVRQLWMNEIDRSLR.V
                     PH.LSIGNCQTKFTHIYFRLI.NFIFNLKGSR.RSFLIIS.PKSLNVSFRSTERQTP.
                     KRSKDLLEILFFCA.SAACKQKNHRYQRWFVCRIKSYQLFFRR.LASAERRYQILFF.
                     CSRS.ATTSRTL.HRLHTSLC.SCYQWLLPVAISRVLPGWTQDDSYRIRRSGRAERGV
                     RAHSPAWSERPTPN.DTYSVSYEKAPRFPKGERRTGIR.AAGSEQESARGSFQGETPG
                     IFIVLSGFATSDLSVDFCDARQGGGAYGKTPATRPFYGSWPFAGLLLTCSFLRYPLIL
                     WITVLPPLSELIPLAAAERPSAASQ.ARKRKSAQYANRLSPRVGRFINAAGTTGFPTG
                     KRAVSATQLM.VSSLIRHPRLYTLCFRLVCCVEL.ADNNFTQETAMTMITPSAQLTLT
                     KGNKSWSCKLNVVLCNTLVVLQHGNDELATCLTRREKAPCMPIGGSKVVRSCLIRKAT
                     DGSDMDWTNH.IAALQRYCI.VPSSIHKRVSLVRPDLSLGALWLTREPTA.ASIKLAL
                     SASSSVCPSVV.LW.LEIPQTLLVSVENL.QWRPNRDLKAKGKPEELSRRRTRLAEAR
                     TARGEGRRLVSTPKILTSGG.KERDGCESVSIKRGRIRSRWEKIRLRPGGKKKYKLKH
                     IVWASRELERFAVNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTVATLYCVHQR
                     IEIKDTKEALDKIEEEQNKSKTTAQQAAADLQTWRRR"
     misc_feature    8606..8839
                     /label=RRE
                     /translation="RSFVPWVLGSSRKHYGRSVNDADGTGQTIIVWYSAAAEQFAEGY
                     .GATASVATHSLGHQAAPGKNPGCGKIPKGSTAP"
     misc_feature    220..238
                     /note="tetO"
     misc_feature    256..274
                     /note="tetO"
     misc_feature    291..309
                     /note="tetO"
     misc_feature    327..345
                     /note="tetO"
     misc_feature    363..381
                     /note="tetO"
     misc_feature    398..416
                     /note="tetO"
     misc_feature    434..452
                     /note="tetO"
     misc_feature    complement(2287..2306)
                     /note="OPGK1b-R primer"
     CDS             1306..2052
                     /note="human SHLD3"
                     /translation="TTEVILHYRPCESDPTQLPKIAEKAIQDFPTRPLSRFIPWFPYD
                     GSKLPLRPKRSPPVISEEAAEDVKQYLTISEHDAKSHSYDCTVDLLEFQPSLKKQHLT
                     WSHTLKEQTNSGNLGKQSEKGKQHKRRSWSISLPSNNCTKNVSPLSKKLQDSLKALNL
                     HSLYRARWTIEHTICNSQTLEDIWTKLNQIIRHNELPSCNATIQRHLGQIWVFCDIMY
                     CEYVGSLLKGRLALTGKINLFVHKYGVIFSM"
     polyA_signal    2099..2111
                     /dnas_title="bGH poly(A) signal [fragment]"
                     /note="first few bases only; from pcDNA5-FRT/TO backbone"
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    547..571
                     /note="final attB1 site"
                     /note="in expression vector"
     misc_feature    2113..2137
                     /note="final attB2 site"
                     /note="in expression vector"
     CDS             580..1296
                     /dnas_title="enhanced GFP"
                     /codon_start=1
                     /product="enhanced GFP"
                     /note="EGFP"
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK"
     CDS             580..2052
                     /note="eGFP-SHLD3"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKGAPTTEVILHYRPCESDPTQLPKIAEKAIQDFPTRPL
                     SRFIPWFPYDGSKLPLRPKRSPPVISEEAAEDVKQYLTISEHDAKSHSYDCTVDLLEF
                     QPSLKKQHLTWSHTLKEQTNSGNLGKQSEKGKQHKRRSWSISLPSNNCTKNVSPLSKK
                     LQDSLKALNLHSLYRARWTIEHTICNSQTLEDIWTKLNQIIRHNELPSCNATIQRHLG
                     QIWVFCDIMYCEYVGSLLKGRLALTGKINLFVHKYGVIFSM"
     misc_feature    212..526
                     /note="Tet-responsive promoter PTight"
                     /note="note=%22tight TRE promoter%22 -
                     note=%22Tet-responsive promoter PTight, consisting of
                     seven - tet operator sequences followed by the minimal
                     CMV - promoter%22 - 212..526"
     CDS             3299..3352
                     /note="T2A"
                     /translation="EGRGSLLTCGDVEENPGP"
                     /note="codon_start=1 - product=%222A peptide from Thosea
                     asigna virus capsid - protein%22 - note=%22T2A%22 -
                     note=%22Eukaryotic ribosomes fail to insert a peptide
                     bond - between the Gly and Pro residues, yielding
                     separate - polypeptides.%22 -
                     translation=%22EGRGSLLTCGDVEEN"
     CDS             3353..4099
                     /note="rtTA-Advanced"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLD
                     MLPADALDDFDLDMLPADALDDFDLDMLPG"
                     /note="codon_start=1 - product=%22improved
                     tetracycline-controlled transactivator%22 -
                     note=%22rtTA-Advanced%22 - note=%22In the Tet-On(R)
                     system, rtTA-Advanced binds to the - Tet-responsive
                     element and stimulates transcription only in - the
                     presence of tetracycline or d"
ORIGIN      
        1 AATTCTCGAC CTCGAGACAA ATGGCAGTAT TCATCCACAA TTTTAAAAGA AAAGGGGGGA
       61 TTGGGGGGTA CAGTGCAGGG GAAAGAATAG TAGACATAAT AGCAACAGAC ATACAAACTA
      121 AAGAATTACA AAAACAAATT ACAAAAATTC AAAATTTTCG GGTTTATTAC AGGGACAGCA
      181 GAGATCCACT TTGGCCGCGA ATCGATATGT CGAGTTTACT CCCTATCAGT GATAGAGAAC
      241 GTATGTCGAG TTTACTCCCT ATCAGTGATA GAGAACGATG TCGAGTTTAC TCCCTATCAG
      301 TGATAGAGAA CGTATGTCGA GTTTACTCCC TATCAGTGAT AGAGAACGTA TGTCGAGTTT
      361 ACTCCCTATC AGTGATAGAG AACGTATGTC GAGTTTATCC CTATCAGTGA TAGAGAACGT
      421 ATGTCGAGTT TACTCCCTAT CAGTGATAGA GAACGTATGT CGAGGTAGGC GTGTACGGTG
      481 GGAGGCCTAT ATAAGCAGAG CTCGTTTAGT GAACCGTCAG ATCGCCTGGA GAATTGGCTA
      541 GCATCAACAA GTTTgtacaa aaaagcaggc tCCggtacca tggtgagcaa gggcgaggag
      601 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
      661 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
      721 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
      781 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
      841 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
      901 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
      961 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     1021 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     1081 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     1141 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
     1201 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     1261 gccgggatca ctctcggcat ggacgagctg tacaagggcg cgccaACTAC AGAAGTAATA
     1321 TTACATTATC GACCATGTGA GAGTGATCCC ACACAACTGC CAAAAATTGC AGAAAAAGCA
     1381 ATTCAAGACT TTCCTACTCG TCCGCTATCA AGATTTATAC CTTGGTTTCC ATATGATGGG
     1441 TCCAAGCTTC CACTCAGACC TAAAAGATCA CCACCTGTGA TTTCTGAAGA GGCAGCTGAA
     1501 GATGTGAAAC AGTACTTAAC CATTTCAGAA CATGATGCTA AGTCACACAG TTATGATTGC
     1561 ACAGTAGATC TATTGGAGTT TCAACCTAGC TTGAAAAAGC AGCATTTAAC CTGGTCACAC
     1621 ACACTGAAGG AACAGACTAA TTCTGGAAAT CTGGGTAAAC AATCAGAAAA GGGAAAACAG
     1681 CACAAGAGGA GATCTTGGAG TATTTCCCTT CCCAGCAATA ATTGTACTAA AAACGTTTCT
     1741 CCTTTGTCTA AAAAATTGCA AGATAGTTTA AAGGCACTAA ATTTACACTC ACTTTATAGA
     1801 GCAAGATGGA CAATAGAACA CACTATTTGT AACAGCCAAA CTCTGGAAGA CATTTGGACA
     1861 AAACTCAATC AAATTATTAG GCACAATGAA CTTCCATCTT GTAATGCTAC AATTCAGAGG
     1921 CATTTAGGCC AAATATGGGT GTTCTGTGAT ATTATGTATT GTGAATATGT GGGAAGTCTT
     1981 CTTAAAGGAA GATTAGCTCT TACTGGAAAA ATTAATTTAT TTGTGCATAA ATATGGTGTT
     2041 ATTTTTAGTA TGTAGctcga gtctagaggg cccgtttaaa cccgctgatc agcctcgact
     2101 gtgccttcta gCacccagct ttCTTGTACA AAGTGGTTTA GTAATGAACC GGTCCACCAC
     2161 CACCACCACC ACTAAGGATC CGGGGTTGGG GTTGCGCCTT TTCCAAGGCA GCCCTGGGTT
     2221 TGCGCAGGGA CGCGGCTGCT CTGGGCGTGG TTCCGGGAAA CGCAGCGGCG CCGACCCTGG
     2281 GTCTCGCACA TTCTTCACGT CCGTTCGCAG CGTCACCCGG ATCTTCGCCG CTACCCTTGT
     2341 GGGCCCCCCG GCGACGCTTC CTGCTCCGCC CCTAAGTCGG GAAGGTTCCT TGCGGTTCGC
     2401 GGCGTGCCGG ACGTGACAAA CGGAAGCCGC ACGTCTCACT AGTACCCTCG CAGACGGACA
     2461 GCGCCAGGGA GCAATGGCAG CGCGCCGACC GCGATGGGCT GTGGCCAATA GCGGCTGCTC
     2521 AGCAGGGCGC GCCGAGAGCA GCGGCCGGGA AGGGGCGGTG CGGGAGGCGG GGTGTGGGGC
     2581 GGTAGTGTGG GCCCTGTTCC TGCCCGCGCG GTGTTCCGCA TTCTGCAAGC CTCCGGAGCG
     2641 CACGTCGGCA GTCGGCTCCC TCGTTGACCG AATCACCGAC CTCTCTCCCC AGCAATTCAC
     2701 CATGACCGAG TACAAGCCCA CGGTGCGCCT CGCCACCCGC GACGACGTCC CCAGGGCCGT
     2761 ACGCACCCTC GCCGCCGCGT TCGCCGACTA CCCCGCCACG CGCCACACCG TCGATCCGGA
     2821 CCGCCACATC GAGCGGGTCA CCGAGCTGCA AGAACTCTTC CTCACGCGCG TCGGGCTCGA
     2881 CATCGGCAAG GTGTGGGTCG CGGACGACGG CGCCGCGGTG GCGGTCTGGA CCACGCCGGA
     2941 GAGCGTCGAA GCGGGGGCGG TGTTCGCCGA GATCGGCCCG CGCATGGCCG AGTTGAGCGG
     3001 TTCCCGGCTG GCCGCGCAGC AACAGATGGA AGGCCTCCTG GCGCCGCACC GGCCCAAGGA
     3061 GCCCGCGTGG TTCCTGGCCA CCGTCGGCGT CTCGCCCGAC CACCAGGGCA AGGGTCTGGG
     3121 CAGCGCCGTC GTGCTCCCCG GAGTGGAGGC GGCCGAGCGC GCCGGGGTGC CCGCCTTCCT
     3181 GGAGACCTCC GCGCCCCGCA ACCTCCCCTT CTACGAGCGG CTCGGCTTCA CCGTCACCGC
     3241 CGACGTCGAG GTGCCCGAAG GACCGCGCAC CTGGTGCATG ACCCGCAAGC CCGGTGCCGA
     3301 AGGTAGAGGT TCTCTCCTCA CTTGTGGTGA TGTTGAAGAA AACCCTGGTC CAATGTCTAG
     3361 ACTGGACAAG AGCAAAGTCA TAAACGGAGC TCTGGAATTA CTCAATGGTG TCGGTATCGA
     3421 AGGCCTGACG ACAAGGAAAC TCGCTCAAAA GCTGGGAGTT GAGCAGCCTA CCCTGTACTG
     3481 GCACGTGAAG AACAAGCGGG CCCTGCTCGA TGCCCTGCCA ATCGAGATGC TGGACAGGCA
     3541 TCATACCCAC TTCTGCCCCC TGGAAGGCGA GTCATGGCAA GACTTTCTGC GGAACAACGC
     3601 CAAGTCATAC CGCTGTGCTC TCCTCTCACA TCGCGACGGG GCTAAAGTGC ATCTCGGCAC
     3661 CCGCCCAACA GAGAAACAGT ACGAAACCCT GGAAAATCAG CTCGCGTTCC TGTGTCAGCA
     3721 AGGCTTCTCC CTGGAGAACG CACTGTACGC TCTGTCCGCC GTGGGCCACT TTACACTGGG
     3781 CTGCGTATTG GAGGAACAGG AGCATCAAGT AGCAAAAGAG GAAAGAGAGA CACCTACCAC
     3841 CGATTCTATG CCCCCACTTC TGAGACAAGC AATTGAGCTG TTCGACCGGC AGGGAGCCGA
     3901 ACCTGCCTTC CTTTTCGGCC TGGAACTAAT CATATGTGGC CTGGAGAAAC AGCTAAAGTG
     3961 CGAAAGCGGC GGGCCGACCG ACGCCCTTGA CGATTTTGAC TTAGACATGC TCCCAGCCGA
     4021 TGCCCTTGAC GACTTTGACC TTGATATGCT GCCTGCTGAC GCTCTTGACG ATTTTGACCT
     4081 TGACATGCTC CCCGGGTAAC TAAGTAAGGA TCGATCCAAG ATATCGTATT CTTAACTATG
     4141 TTGCTCCTTT TACGCTATGT GGATACGCTG CTTTAATGCC TTTGTATCAT GCTATTGCTT
     4201 CCCGTATGGC TTTCATTTTC TCCTCCTTGT ATAAATCCTG GTTGCTGTCT CTTTATGAGG
     4261 AGTTGTGGCC CGTTGTCAGG CAACGTGGCG TGGTGTGCAC TGTGTTTGCT GACGCAACCC
     4321 CCACTGGTTG GGGCATTGCC ACCACCTGTC AGCTCCTTTC CGGGACTTTC GCTTTCCCCC
     4381 TCCCTATTGC CACGGCGGAA CTCATCGCCG CCTGCCTTGC CCGCTGCTGG ACAGGGGCTC
     4441 GGCTGTTGGG CACTGACAAT TCCGTGGTGT TGTCGGGGAA GCTGACGTCC TTTCCATGGC
     4501 TGCTCGCCTG TGTTGCCACC TGGATTCTGC GCGGGACGTC CTTCTGCTAC GTCCCTTCGG
     4561 CCCTCAATCC AGCGGACCTT CCTTCCCGCG GCCTGCTGCC GGCTCTGCGG CCTCTTCCGC
     4621 GTCTTCGCCT TCGCCCTCAG ACGAGTCGGA TCTCCCTTTG GGCCGCCTCC CCGCCTGTTT
     4681 CGCCTCGGCG TCCGGACTAG AGGTACCTTT AAGACCAATG ACTTACAAGG CAGCTGTAGA
     4741 TCTTAGCCAC TTTTTAAAAG AAAAGGGGGG ACTGGAAGGG CTAATTCACT CCCAACGAAG
     4801 ACAAGATCTG CTTTTTGCTT GTACTGGGTC TCTCTGGTTA GACCAGATCT GAGCCTGGGA
     4861 GCTCTCTGGC TAACTAGGGA ACCCACTGCT TAAGCCTCAA TAAAGCTTGC CTTGAGTGCT
     4921 TCAAGTAGTG TGTGCCCGTC TGTTGTGTGA CTCTGGTAAC TAGAGATCCC TCAGACCCTT
     4981 TTAGTCAGTG TGGAAAATCT CTAGCAGTAG TAGTTCATGT CATCTTATTA TTCAGTATTT
     5041 ATAACTTGCA AAGAAATGAA TATCAGAGAG TGAGAGGAAC TTGTTTATTG CAGCTTATAA
     5101 TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA
     5161 TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGGC TCTAGCTATC
     5221 CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC
     5281 CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA
     5341 TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTTTCAA ATATGTATCC GCTCATGAGA
     5401 CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT
     5461 TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA
     5521 GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC
     5581 GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA
     5641 ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT TGACGCCGGG
     5701 CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA
     5761 GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA
     5821 ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG
     5881 CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG
     5941 GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT AGCAATGGCA
     6001 ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA
     6061 ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT
     6121 GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA
     6181 GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG
     6241 GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT
     6301 TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT
     6361 TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA
     6421 CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA
     6481 GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG
     6541 GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC
     6601 AGAGCGCAGA TACCAAATAC TGTTCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG
     6661 AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC
     6721 AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG
     6781 CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC
     6841 ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA
     6901 AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT
     6961 CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG
     7021 CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG
     7081 GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA
     7141 TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC
     7201 AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC
     7261 AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC
     7321 GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA
     7381 CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA
     7441 CAATTTCACA CAGGAAACAG CTATGACCAT GATTACGCCA AGCGCGCAAT TAACCCTCAC
     7501 TAAAGGGAAC AAAAGCTGGA GCTGCAAGCT TAATGTAGTC TTATGCAATA CTCTTGTAGT
     7561 CTTGCAACAT GGTAACGATG AGTTAGCAAC ATGCCTTACA AGGAGAGAAA AAGCACCGTG
     7621 CATGCCGATT GGTGGAAGTA AGGTGGTACG ATCGTGCCTT ATTAGGAAGG CAACAGACGG
     7681 GTCTGACATG GATTGGACGA ACCACTGAAT TGCCGCATTG CAGAGATATT GTATTTAAGT
     7741 GCCTAGCTCG ATACATAAAC GGGTCTCTCT GGTTAGACCA GATCTGAGCC TGGGAGCTCT
     7801 CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG CTTGCCTTGA GTGCTTCAAG
     7861 TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG ATCCCTCAGA CCCTTTTAGT
     7921 CAGTGTGGAA AATCTCTAGC AGTGGCGCCC GAACAGGGAC TTGAAAGCGA AAGGGAAACC
     7981 AGAGGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA GAGGCGAGGG
     8041 GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG AGAGAGATGG
     8101 GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA AAATTCGGTT
     8161 AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA GCAGGGAGCT
     8221 AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA GACAAATACT
     8281 GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT TATATAATAC
     8341 AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA AGGAAGCTTT
     8401 AGACAAGATA GAGGAAGAGC AAAACAAAAG TAAGACCACC GCACAGCAAG CGGCCGCTGA
     8461 TCTTCAGACC TGGAGGAGGA GATATGAGGG ACAATTGGAG AAGTGAATTA TATAAATATA
     8521 AAGTAGTAAA AATTGAACCA TTAGGAGTAG CACCCACCAA GGCAAAGAGA AGAGTGGTGC
     8581 AGAGAGAAAA AAGAGCAGTG GGAATAGGAG CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG
     8641 GAAGCACTAT GGGCGCAGCG TCAATGACGC TGACGGTACA GGCCAGACAA TTATTGTCTG
     8701 GTATAGTGCA GCAGCAGAAC AATTTGCTGA GGGCTATTGA GGCGCAACAG CATCTGTTGC
     8761 AACTCACAGT CTGGGGCATC AAGCAGCTCC AGGCAAGAAT CCTGGCTGTG GAAAGATACC
     8821 TAAAGGATCA ACAGCTCCTG GGGATTTGGG GTTGCTCTGG AAAACTCATT TGCACCACTG
     8881 CTGTGCCTTG GAATGCTAGT TGGAGTAATA AATCTCTGGA ACAGATTTGG AATCACACGA
     8941 CCTGGATGGA GTGGGACAGA GAAATTAACA ATTACACAAG CTTAATACAC TCCTTAATTG
     9001 AAGAATCGCA AAACCAGCAA GAAAAGAATG AACAAGAATT ATTGGAATTA GATAAATGGG
     9061 CAAGTTTGTG GAATTGGTTT AACATAACAA ATTGGCTGTG GTATATAAAA TTATTCATAA
     9121 TGATAGTAGG AGGCTTGGTA GGTTTAAGAA TAGTTTTTGC TGTACTTTCT ATAGTGAATA
     9181 GAGTTAGGCA GGGATATTCA CCATTATCGT TTCAGACCCA CCTCCCAACC CCGAGGGGAC
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