LOCUS       pCW-eGFP-RNF8_FH        8932 bp    DNA     circular     12-JUL-2018
FEATURES             Location/Qualifiers
     misc_feature    43..160
                     /note="central polypurine tract and central terminatio..."
                     /note="note=%22cPPT/CTS%22 - note=%22central polypurine
                     tract and central termination - sequence of HIV-1%22 -
                     41..158"
     promoter        214..528
                     /note="Tet-responsive promoter PTight, consisting of s..."
                     /note="note=%22tight TRE promoter%22 -
                     note=%22Tet-responsive promoter PTight, consisting of
                     seven - tet operator sequences followed by the minimal
                     CMV - promoter%22 - 212..526"
     protein_bind    222..240
                     /note="bacterial operator O2 for the tetR and tetA gen..."
                     /note="gene=%22tetO%22 - bound_moiety=%22tetracycline
                     repressor TetR%22 - note=%22tet operator%22 -
                     note=%22bacterial operator O2 for the tetR and tetA
                     genes%22 - 434..452"
     protein_bind    258..276
                     /note="bacterial operator O2 for the tetR and tetA gen..."
                     /note="gene=%22tetO%22 - bound_moiety=%22tetracycline
                     repressor TetR%22 - note=%22tet operator%22 -
                     note=%22bacterial operator O2 for the tetR and tetA
                     genes%22 - 434..452"
     protein_bind    293..311
                     /note="bacterial operator O2 for the tetR and tetA gen..."
                     /note="gene=%22tetO%22 - bound_moiety=%22tetracycline
                     repressor TetR%22 - note=%22tet operator%22 -
                     note=%22bacterial operator O2 for the tetR and tetA
                     genes%22 - 434..452"
     protein_bind    329..347
                     /note="bacterial operator O2 for the tetR and tetA gen..."
                     /note="gene=%22tetO%22 - bound_moiety=%22tetracycline
                     repressor TetR%22 - note=%22tet operator%22 -
                     note=%22bacterial operator O2 for the tetR and tetA
                     genes%22 - 434..452"
     protein_bind    365..383
                     /note="bacterial operator O2 for the tetR and tetA gen..."
                     /note="gene=%22tetO%22 - bound_moiety=%22tetracycline
                     repressor TetR%22 - note=%22tet operator%22 -
                     note=%22bacterial operator O2 for the tetR and tetA
                     genes%22 - 434..452"
     protein_bind    400..418
                     /note="bacterial operator O2 for the tetR and tetA gen..."
                     /note="gene=%22tetO%22 - bound_moiety=%22tetracycline
                     repressor TetR%22 - note=%22tet operator%22 -
                     note=%22bacterial operator O2 for the tetR and tetA
                     genes%22 - 434..452"
     protein_bind    436..454
                     /note="bacterial operator O2 for the tetR and tetA gen..."
                     /note="gene=%22tetO%22 - bound_moiety=%22tetracycline
                     repressor TetR%22 - note=%22tet operator%22 -
                     note=%22bacterial operator O2 for the tetR and tetA
                     genes%22 - 434..452%22 556..1822"
                     /note="note=%22Fragment inserted from
                     pDONR221-eGFP-RNF8_FHA.txt.xdna into pCW57.1.gb.xdna [LR]
                     reaction"
     CDS             1849..1866
                     /label=6xHis_CDS
                     /note="6xHis_CDS"
                     /note="codon_start=1 - product=%226xHis affinity tag%22 -
                     note=%226xHis%22 - translation=%22HHHHHH%22 - 2269..2286"
     promoter        1876..2386
                     /note="human phosphoglycerate kinase 1 promoter_hPGK p..."
                     /note="note=%22hPGK promoter%22 - note=%22human
                     phosphoglycerate kinase 1 promoter%22 - 2296..2806"
     CDS             2993..3046
                     /dnas_title="T2A"
                     /note="T2A"
                     /note="codon_start=1 - product=%222A peptide from Thosea
                     asigna virus capsid - protein%22 - note=%22T2A%22 -
                     note=%22Eukaryotic ribosomes fail to insert a peptide
                     bond - between the Gly and Pro residues, yielding
                     separate - polypeptides.%22 -
                     translation=%22EGRGSLLTCGDVEEN"
     CDS             3047..3793
                     /dnas_title="rtTA-Advanced"
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to..."
                     /note="codon_start=1 - product=%22improved
                     tetracycline-controlled transactivator%22 -
                     note=%22rtTA-Advanced%22 - note=%22In the Tet-On(R)
                     system, rtTA-Advanced binds to the - Tet-responsive
                     element and stimulates transcription only in - the
                     presence of tetracycline or d"
                     /note="rtTA-Advanced"
     CDS             complement(4251..4262)
                     /label=Factor Xa site_CDS
                     /note="Factor Xa site_CDS"
                     /note="codon_start=1 - product=%22Factor Xa recognition
                     and cleavage site%22 - note=%22Factor Xa site%22 -
                     translation=%22IEGR%22 - complement(4671..4682)"
     LTR             4467..4700
                     /note="self-inactivating 3' long terminal repeat (LTR)..."
                     /note="note=%223' LTR (Delta-U3)%22 -
                     note=%22self-inactivating 3' long terminal repeat (LTR)
                     from - HIV-1%22 - 4887..5120"
     LTR             4520..4700
                     /note="truncated 5' long terminal repeat (LTR) from HI..."
                     /note="note=%225' LTR (truncated)%22 - note=%22truncated
                     5' long terminal repeat (LTR) from HIV-1%22 - 7875..8055"
     polyA_signal    4772..4893
                     /note="SV40 polyadenylation signal_SV40 poly(A) signal..."
                     /note="note=%22SV40 poly(A) signal%22 - note=%22SV40
                     polyadenylation signal%22 - 5192..5313"
     rep_origin      4933..5068
                     /direction=RIGHT
                     /label=SV40 origin of replication_SV40 ori_rep_origin
                     /note="SV40 origin of replication_SV40 ori_rep_origin"
                     /note="note=%22SV40 ori%22 - note=%22SV40 origin of
                     replication%22 - 5353..5488"
     promoter        5068..5139
                     /note="AmpR promoter_bla_promoter"
                     /note="gene=%22bla%22 - note=%22AmpR promoter%22 -
                     5488..5559"
     protein_bind    7047..7068
                     /note="CAP binding activates transcription in the pres..."
                     /note="bound_moiety=%22E. coli catabolite activator
                     protein%22 - note=%22CAP binding site%22 - note=%22CAP
                     binding activates transcription in the presence - of
                     cAMP.%22 - 7467..7488"
     promoter        7083..7113
                     /note="promoter for the E. coli lac operon_lac promote..."
                     /note="note=%22lac promoter%22 - note=%22promoter for the
                     E. coli lac operon%22 - 7503..7533"
     protein_bind    7121..7137
                     /note="The lac repressor binds to the lac operator to ..."
                     /note="bound_moiety=%22lac repressor encoded by lacI%22 -
                     note=%22lac operator%22 - note=%22The lac repressor binds
                     to the lac operator to - inhibit transcription in E.
                     coli. This inhibition can be - relieved by adding lactose
                     or - isopropyl-beta-D-thiogalactopyranosid"
     primer_bind     7145..7161
                     /note="common sequencing primer, one of multiple simil..."
                     /note="note=%22M13 rev%22 - note=%22common sequencing
                     primer, one of multiple similar - variants%22 -
                     complement(3027..3043)"
     promoter        7182..7200
                     /note="promoter for bacteriophage T3 RNA polymerase_T3..."
                     /note="note=%22T3 promoter%22 - note=%22promoter for
                     bacteriophage T3 RNA polymerase%22 - 7602..7620"
     promoter        7228..7454
                     /note="Rous sarcoma virus enhancer/promoter_RSV promot..."
                     /note="note=%22RSV promoter%22 - note=%22Rous sarcoma
                     virus enhancer/promoter%22 - 7648..7874"
     LTR             7455..7635
                     /note="truncated 5' long terminal repeat (LTR) from HI..."
                     /note="note=%225' LTR (truncated)%22 - note=%22truncated
                     5' long terminal repeat (LTR) from HIV-1%22 - 7875..8055"
     misc_feature    7682..7807
                     /note="packaging signal of human immunodeficiency viru..."
                     /note="note=%22HIV-1 Psi%22 - note=%22packaging signal of
                     human immunodeficiency virus - type 1%22 - 8102..8227"
     misc_feature    8300..8533
                     /note="The Rev response element (RRE) of HIV-1 allows ..."
                     /note="note=%22RRE%22 - note=%22The Rev response element
                     (RRE) of HIV-1 allows for - Rev-dependent mRNA export
                     from the nucleus to the - cytoplasm.%22 - 8720..8953"
     CDS             591..1802
                     /note="eGFP-RNF8_FHA"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKGAPAGAGEPGFFVTGDRAGGRSWCLRRVGMSAGWLLL
                     EDGCEVTVGRGFGVTYQLVSKICPLMISRNHCVLKQNPEGQWTIMDNKSLNGVWLNRA
                     RLEPLRVYSIHQGDYIQLGVPLENKENAEYEYEVTEEDWETIYPCLSPKNDQMIEKNK
                     ELRTKRKFSLDE"
     misc_feature    47..62
                     /dnas_title="cPPT"
                     /label=cPPT
     misc_feature    222..240
                     /dnas_title="tetO"
                     /note="tetO"
     misc_feature    258..276
                     /dnas_title="tetO"
                     /note="tetO"
     misc_feature    293..311
                     /dnas_title="tetO"
                     /note="tetO"
     misc_feature    329..347
                     /dnas_title="tetO"
                     /note="tetO"
     misc_feature    365..383
                     /dnas_title="tetO"
                     /note="tetO"
     misc_feature    400..418
                     /dnas_title="tetO"
                     /note="tetO"
     misc_feature    436..454
                     /dnas_title="tetO"
                     /note="tetO"
     misc_feature    499..518
                     /label=pCEP_fwd_primer
     misc_feature    501..525
                     /label=LNCX primer
     misc_feature    549..573
                     /dnas_title="final attB1 site"
                     /note="final attB1 site"
                     /note="in expression clone"
     misc_recomb     549..573
                     /dnas_title="attB1"
                     /note="attB1"
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             591..1307
                     /dnas_title="enhanced GFP"
                     /codon_start=1
                     /product="enhanced GFP"
                     /note="EGFP"
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK"
     CDS             1326..1802
                     /dnas_title="RNF8 FHA"
                     /note="RNF8 FHA"
                     /translation="GEPGFFVTGDRAGGRSWCLRRVGMSAGWLLLEDGCEVTVGRGFG
                     VTYQLVSKICPLMISRNHCVLKQNPEGQWTIMDNKSLNGVWLNRARLEPLRVYSIHQG
                     DYIQLGVPLENKENAEYEYEVTEEDWETIYPCLSPKNDQMIEKNKELRTKRKFSLDE"
     misc_feature    1807..1831
                     /note="final attB2 site"
                     /note="in expression clone"
     misc_recomb     complement(1807..1831)
                     /dnas_title="attB2"
                     /note="attB2"
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    complement(1981..2000)
                     /note="OPGK1b-R primer"
     gene            2396..2983
                     /dnas_title="PuroR"
                     /label=puro (variant)
                     /gene="puro (variant)"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
                     ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRK"
     misc_feature    3830..4357
                     /label=WPRE
                     /translation="LCCSFYAMWIRCFNAFVSCYCFPYGFHFLLLV.ILVAVSL.GVV
                     ARCQATWRGVHCVC.RNPHWLGHCHHLSAPFRDFRFPPPYCHGGTHRRLPCPLLDRGS
                     AVGH.QFRGVVGEADVLSMAARLCCHLDSARDVLLLRPFGPQSSGPSFPRPAAGSAAS
                     SASSPSPSDESDLPLG"
     misc_feature    4450..4471
                     /dnas_title="U3PPT"
                     /label=U3PPT
                     /translation="KRKGGTG"
     misc_feature    4450..4465
                     /dnas_title="cPPT"
                     /label=cPPT
     misc_feature    4467..4519
                     /label=delta_U3
                     /translation="WKG.FTPNEDKICFLLV"
     repeat_region   4467..4701
                     /dnas_title="HIV-1 3' LTR"
                     /rpt_type=long_terminal_repeat
                     /note="HIV-1 deltaU3 3' LTR"
     misc_feature    4520..4700
                     /dnas_title="HIV-1_5_LTR"
                     /label=HIV-1_5_LTR
                     /translation="GSLWLDQI.AWELSG.LGNPLLKPQ.SLP.VLQVVCARLLCDSG
                     N.RSLRPF.SVWKISS"
     repeat_region   4520..4700
                     /dnas_title="HIV-1 5' LTR"
                     /rpt_type=long_terminal_repeat
                     /note="HIV-1 5' LTR"
     terminator      4775..4894
                     /label=SV40_PA_terminator
                     /translation="LFIAAYNGYK.SNSITNFTNKAFFSLHSSCGLSKLINVSY"
     regulatory      4828..4902
                     /dnas_title="SV40 early polyA signal"
                     /regulatory_class="polyA_signal_sequence"
                     /note="SV40 early polyadenylation signal"
                     /note="the early polyadenylation signal (EPA) and the
                     late polyadenylation signal (LPA) are on opposite strands
                     of the same region"
     misc_feature    4863..4882
                     /label=EBV_rev_primer
                     /translation="VVCPNS"
     rep_origin      4912..5010
                     /label=SV40_origin
                     /translation="IPPLTPPIPPLTPPSSAHSPPHG.LIFFIYAEA"
     rep_origin      4985..5048
                     /dnas_title="SV40 ori"
                     /note="SV40 origin of replication"
                     /note="this core origin is both necessary and sufficient
                     for replication"
     misc_feature    4995..5014
                     /dnas_title="SV40pro_F_primer"
                     /label=SV40pro_F_primer
                     /translation="YLCRGR"
     promoter        5070..5098
                     /label=AmpR_promoter
                     /translation="FKYVSAHET"
     regulatory      5070..5105
                     /dnas_title="AmpR promoter"
                     /regulatory_class="promoter"
                     /note="natural promoter for the beta-lactamase gene"
                     /note="includes -35, -10, and transcription start site"
     gene            5140..6000
                     /dnas_title="Ampicillin"
                     /label=Ampicillin
                     /gene="Ampicillin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     rep_origin      6155..6774
                     /label=pBR322_origin
                     /translation="RSKDLLEILFFCA.SAACKQKNHRYQRWFVCRIKSYQLFFRR.L
                     ASAERRYQILFF.CSRS.ATTSRTL.HRLHTSLC.SCYQWLLPVAISRVLPGWTQDDS
                     YRIRRSGRAERGVRAHSPAWSERPTPN.DTYSVSYEKAPRFPKGERRTGIR.AAGSEQ
                     ESARGSFQGETPGIFIVLSGFATSDLSVDFCDARQGGGAYGKTPAT"
     regulatory      7048..7072
                     /dnas_title="CAP binding site"
                     /regulatory_class="transcriptional_cis_regulatory_region"
                     /note="CAP binding site"
     regulatory      7083..7113
                     /dnas_title="lac promoter"
                     /regulatory_class="promoter"
                     /note="lac promoter"
     promoter        7083..7112
                     /dnas_title="lac_promoter"
                     /label=lac_promoter
                     /translation="FTLYASGSYV"
     regulatory      7119..7139
                     /dnas_title="lac operator"
                     /regulatory_class="transcriptional_cis_regulatory_region"
                     /note="Lac operator"
                     /note="Lac repressor binding site"
     misc_feature    7126..7148
                     /label=M13_pUC_rev_primer
                     /translation="SG.QFHT"
     promoter        7147..7165
                     /label=M13_reverse_primer
                     /translation="GNSYDH"
     CDS             7157..7171
                     /dnas_title="lacZa part A"
                     /gene="lacZ alpha"
                     /codon_start=1
                     /transl_table=11
                     /product="Beta-galactosidase alpha peptide"
                     /translation="MTMIT"
     promoter        7182..7201
                     /dnas_title="T3_promoter"
                     /label=T3_promoter
                     /translation="N.PSLK"
     regulatory      7182..7199
                     /dnas_title="T3 promoter"
                     /regulatory_class="promoter"
                     /note="T3 promoter"
                     /note="bacteriophage T3 RNA polymerase promoter"
     regulatory      7226..7454
                     /dnas_title="RSV promoter"
                     /regulatory_class="promoter"
                     /note="RSV promoter"
     regulatory      7226..7635
                     /dnas_title="RSV LTR promoter"
                     /regulatory_class="promoter"
                     /note="RSV/5' LTR hybrid promoter"
                     /note="hybrid of the Rous Sarcoma virus promoter and the
                     HIV 5' LTR"
     misc_feature    7455..7635
                     /dnas_title="HIV-1_5_LTR"
                     /label=HIV-1_5_LTR
                     /translation="GSLWLDQI.AWELSG.LGNPLLKPQ.SLP.VLQVVCARLLCDSG
                     N.RSLRPF.SVWKISS"
     repeat_region   7455..7635
                     /dnas_title="HIV-1 5' LTR"
                     /rpt_type=long_terminal_repeat
                     /note="HIV-1 5' LTR"
     misc_feature    7637..7654
                     /dnas_title="HIV-1 PBS"
                     /note="primer binding site (PBS)"
                     /note="complementary to the 3%E2%80^2-end of tRNA-Lys3"
     misc_feature    7690..7809
                     /dnas_title="HIV-1 psi"
                     /note="HIV-1 psi (psi) packaging signal"
                     /note="contains SL1, SL2, SL3, and SL4 stem-loops"
     misc_feature    7746..7790
                     /dnas_title="HIV-1_psi_pack"
                     /label=HIV-1_psi_pack
                     /translation=".VRQKF.LAEARRRE"
     source          1..8932
                     /dnas_title="pCW-eGFP-RNF8_FHA for Addgene"
ORIGIN      
        1 acaattctcg acctcgagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
       61 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
      121 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
      181 cagagatcca ctttggccgc gaatcgatat gtcgagttta ctccctatca gtgatagaga
      241 acgtatgtcg agtttactcc ctatcagtga tagagaacga tgtcgagttt actccctatc
      301 agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg tatgtcgagt
      361 ttactcccta tcagtgatag agaacgtatg tcgagtttat ccctatcagt gatagagaac
      421 gtatgtcgag tttactccct atcagtgata gagaacgtat gtcgaggtag gcgtgtacgg
      481 tgggaggcct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagaattggc
      541 tagcatcaac aagtttgtac aaaaaagcag gcttcgaagg agatagaacc atggtgagca
      601 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
      661 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
      721 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
      781 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
      841 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
      901 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
      961 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     1021 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     1081 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     1141 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     1201 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     1261 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagggc gcgccagccg
     1321 gagccgggga gcccggcttc ttcgtcacag gagaccgcgc cggtggccgg agctggtgcc
     1381 tgcggcgggt ggggatgagc gccgggtggc tgctgctgga agatgggtgc gaggtgactg
     1441 taggacgagg atttggtgtc acataccaac tggtatcaaa aatctgcccc ctgatgattt
     1501 ctcgaaacca ctgtgttttg aagcagaatc ctgagggcca atggacaatt atggacaaca
     1561 agagtctaaa tggtgtttgg ctgaacagag cgcgtctgga acctttaagg gtctattcca
     1621 ttcatcaggg agactacatc caacttggag tgcctctgga aaataaggag aatgcggagt
     1681 atgaatatga agttactgaa gaagactggg agacaatata tccttgtctt tccccaaaga
     1741 atgaccaaat gatagaaaaa aataaggaat tgagaactaa aaggaaattc agtttggatg
     1801 aataggaccc agctttcttg tacaaagtgg tttagtaatg aaccggtcca ccaccaccac
     1861 caccactaag gatccggggt tggggttgcg ccttttccaa ggcagccctg ggtttgcgca
     1921 gggacgcggc tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg
     1981 cacattcttc acgtccgttc gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc
     2041 cccggcgacg cttcctgctc cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg
     2101 ccggacgtga caaacggaag ccgcacgtct cactagtacc ctcgcagacg gacagcgcca
     2161 gggagcaatg gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct gctcagcagg
     2221 gcgcgccgag agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt
     2281 gtgggccctg ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc
     2341 ggcagtcggc tccctcgttg accgaatcac cgacctctct ccccagcaat tcaccatgac
     2401 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
     2461 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
     2521 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     2581 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
     2641 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     2701 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     2761 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     2821 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     2881 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     2941 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg ccgaaggtag
     3001 aggttctctc ctcacttgtg gtgatgttga agaaaaccct ggtccaatgt ctagactgga
     3061 caagagcaaa gtcataaacg gagctctgga attactcaat ggtgtcggta tcgaaggcct
     3121 gacgacaagg aaactcgctc aaaagctggg agttgagcag cctaccctgt actggcacgt
     3181 gaagaacaag cgggccctgc tcgatgccct gccaatcgag atgctggaca ggcatcatac
     3241 ccacttctgc cccctggaag gcgagtcatg gcaagacttt ctgcggaaca acgccaagtc
     3301 ataccgctgt gctctcctct cacatcgcga cggggctaaa gtgcatctcg gcacccgccc
     3361 aacagagaaa cagtacgaaa ccctggaaaa tcagctcgcg ttcctgtgtc agcaaggctt
     3421 ctccctggag aacgcactgt acgctctgtc cgccgtgggc cactttacac tgggctgcgt
     3481 attggaggaa caggagcatc aagtagcaaa agaggaaaga gagacaccta ccaccgattc
     3541 tatgccccca cttctgagac aagcaattga gctgttcgac cggcagggag ccgaacctgc
     3601 cttccttttc ggcctggaac taatcatatg tggcctggag aaacagctaa agtgcgaaag
     3661 cggcgggccg accgacgccc ttgacgattt tgacttagac atgctcccag ccgatgccct
     3721 tgacgacttt gaccttgata tgctgcctgc tgacgctctt gacgattttg accttgacat
     3781 gctccccggg taactaagta aggatcgatc caagatatcg tattcttaac tatgttgctc
     3841 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     3901 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     3961 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     4021 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     4081 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     4141 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg
     4201 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     4261 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     4321 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct gtttcgcctc
     4381 ggcgtccgga ctagaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     4441 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     4501 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     4561 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     4621 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     4681 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     4741 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
     4801 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     4861 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
     4921 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     4981 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     5041 aagtagtgag gaggcttttt tggaggcctt tcaaatatgt atccgctcat gagacaataa
     5101 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     5161 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     5221 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     5281 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     5341 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     5401 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     5461 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     5521 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     5581 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     5641 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     5701 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     5761 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     5821 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     5881 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     5941 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     6001 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     6061 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     6121 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     6181 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     6241 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     6301 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     6361 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     6421 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     6481 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     6541 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     6601 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     6661 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     6721 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     6781 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     6841 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     6901 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     6961 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     7021 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     7081 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     7141 cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg
     7201 gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca
     7261 acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc
     7321 gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga
     7381 catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag
     7441 ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct
     7501 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     7561 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     7621 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
     7681 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
     7741 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
     7801 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
     7861 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
     7921 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     7981 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     8041 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     8101 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca
     8161 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
     8221 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
     8281 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
     8341 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
     8401 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
     8461 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
     8521 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
     8581 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
     8641 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
     8701 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
     8761 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
     8821 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
     8881 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg gg
//

