LOCUS Exported 7139 bp ds-DNA circular SYN 28-APR-2015 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7139) AUTHORS . TITLE Direct Submission JOURNAL Exported Jul 15, 2018 from SnapGene 4.2.0 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7139 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 863..881 /label=T7 CDS 897..1925 /label=mouse PKA-CAT-alpha Sperm alternative splice form misc_feature 900..917 /label=splicing difference misc_feature 1697 /label=acutally A, silent mutation misc_feature 1962..2675 /label=mEGFP ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctaccatgg 901 cttccagctc caacgatgtg aaagagttcc tagccaaagc caaggaagat ttcctgaaaa 961 aatgggagac cccttctcag aatacagccc agttggatca gtttgataga atcaagaccc 1021 ttggcaccgg ctcctttggg cgagtgatgc tggtgaagca caaggagagt gggaaccact 1081 acgccatgaa gatcttagac aagcagaagg tggtgaagct aaagcagatc gagcacactc 1141 tgaatgagaa gcgcatcctg caggccgtca acttcccgtt cctggtcaaa cttgaattct 1201 ccttcaagga caactcaaac ctgtacatgg tcatggagta tgtagctggt ggcgagatgt 1261 tctcccacct acggcggatt ggaaggttca gcgagcccca tgcccgtttc tacgcggcgc 1321 agatcgtcct gacctttgag tatctgcact ccctggacct catctaccgg gacctgaagc 1381 ccgagaatct tctcatcgac cagcagggct atattcaggt gacagacttc ggttttgcca 1441 agcgtgtgaa aggccgtact tggaccttgt gtgggacccc tgagtacttg gcccccgaga 1501 ttatcctgag caaaggctac aacaaggctg tggactggtg ggctctcgga gtcctcatct 1561 acgagatggc tgctggttac ccacccttct tcgctgacca gcctatccag atctatgaga 1621 aaatcgtctc tgggaaggtg cggttcccat cccacttcag ctctgacttg aaggacctgc 1681 tgcggaacct tctgcaggtg gatctcacca agcgctttgg gaacctcaag aacggggtca 1741 atgacatcaa gaaccacaag tggtttgcca cgactgactg gattgccatc tatcagagaa 1801 aggtggaagc tcccttcata ccaaagttta aaggccctgg ggacacgagt aactttgacg 1861 actatgagga ggaagagatc cgggtctcca tcaatgagaa gtgtggcaag gagtttactg 1921 agtttatcga ttatgatgtt cctgattatg caagcttaat gagcaagggc gaggagctgt 1981 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca 2041 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct 2101 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg 2161 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca 2221 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga 2281 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca 2341 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc 2401 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc 2461 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca 2521 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccaagctga 2581 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg 2641 ggatcactct cggcatggac gagctgtaca agtaatctag agggccctat tctatagtgt 2701 cacctaaatg ctagagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct 2761 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt 2821 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg 2881 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg 2941 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat 3001 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg 3061 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc 3121 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg gcatcccttt agggttccga 3181 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt 3241 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat 3301 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat 3361 ttataaggga ttttggggat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa 3421 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct 3481 ccccaggcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga 3541 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca 3601 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat 3661 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc 3721 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag 3781 ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt 3841 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta 3901 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg 3961 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa 4021 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct 4081 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg 4141 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca 4201 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat 4261 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac 4321 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc 4381 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa 4441 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag 4501 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc 4561 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt 4621 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca 4681 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa 4741 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct 4801 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca 4861 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca 4921 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat 4981 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag 5041 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg 5101 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa 5161 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca 5221 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 5281 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 5341 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 5401 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 5461 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 5521 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcaat 5581 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 5641 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 5701 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 5761 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 5821 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 5881 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 5941 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 6001 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 6061 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 6121 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 6181 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 6241 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 6301 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 6361 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 6421 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct 6481 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 6541 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 6601 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 6661 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 6721 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac 6781 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 6841 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 6901 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 6961 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 7021 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 7081 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtc //