LOCUS       Exported                4973 bp ds-DNA     circular SYN 15-JUL-2018
DEFINITION  pEGFPC1-GluR1Ctail.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4973)
  AUTHORS   UNKNOWN
  TITLE     Direct Submission
  JOURNAL   Exported Jul 15, 2018 from SnapGene 4.2.0
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4973
                     /organism="pEGFPC1-GluR1Ctail"
                     /label=Source_1
     promoter        10..562
                     /label=CMV_immearly_promoter
     misc_feature    65..352
                     /label=CAG_enhancer
     misc_feature    519..539
                     /label=CMV_fwd_primer
     promoter        520..589
                     /label=CMV_promoter
     CDS             613..1632
                     /label=ORF frame 1
     misc_structure  613..1329
                     /label=mEGFP (A206K)
     misc_feature    complement(658..679)
                     /label=EGFP_N_primer
     misc_feature    796..825
                     /label=Y66 (EGFP)
     misc_feature    1266..1287
                     /label=EGFP_C_primer
     CDS             1384..1629
                     /label=GluR1-C
     misc_feature    1453..1455
                     /label=CaMKII site S831
     misc_feature    1495..1497
                     /label=PKA site S845
     terminator      1654..1882
                     /label=SV40_PA_terminator
     misc_feature    1852..1871
                     /label=EBV_rev_primer
     rep_origin      complement(2021..2327)
                     /direction=LEFT
                     /label=f1_origin
     promoter        2406..2434
                     /label=AmpR_promoter
     misc_feature    complement(2500..2520)
                     /label=pBABE_3_primer
     misc_feature    complement(2506..2721)
                     /label=SV40_enhancer
     promoter        2518..2786
                     /label=SV40_promoter
     rep_origin      2685..2762
                     /label=SV40_origin
     misc_feature    2747..2766
                     /label=SV40pro_F_primer
     CDS             2869..3663
                     /label=ORF frame 1
     gene            2872..3660
                     /gene="NeoR/KanR"
                     /label=NeoR/KanR
     CDS             complement(3178..3714)
                     /label=ORF frame 3
     terminator      3838..4107
                     /label=TK_PA_terminator
     rep_origin      4255..4874
                     /label=pBR322_origin
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc aagctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgagct caagcttcga attctgcagt cgacgccacc
     1381 atggctgagt tctgctacaa atcccgtagc gagtcgaagc ggatgaaggg tttctgtttg
     1441 atcccacagc aatccatcaa tgaagccata cggacatcga ccctcccccg gaacagtggg
     1501 gcaggagcca gcggaggagg cggcagtgga gagaatggcc gggtggtcag ccaggacttc
     1561 cccaagtcca tgcaatccat tccctgcatg agtcacagtt cagggatgcc cttgggagcc
     1621 acaggattgt aacccgggca tgctctagat aactgatcat aatcagccat accacatttg
     1681 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     1741 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     1801 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     1861 ccaaactcat caatgtatct taacgcgtaa attgtaagcg ttaatatttt gttaaaattc
     1921 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     1981 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     2041 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     2101 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     2161 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     2221 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     2281 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     2341 gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     2401 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     2461 tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt
     2521 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     2581 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     2641 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     2701 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     2761 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     2821 taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
     2881 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
     2941 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
     3001 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca
     3061 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
     3121 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
     3181 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
     3241 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
     3301 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
     3361 ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc
     3421 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     3481 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     3541 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     3601 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     3661 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag
     3721 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     3781 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg
     3841 ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa
     3901 taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt
     3961 cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg
     4021 tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca
     4081 acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt tagattgatt
     4141 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     4201 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     4261 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     4321 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     4381 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     4441 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     4501 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     4561 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     4621 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     4681 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     4741 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     4801 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     4861 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     4921 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg cat
//
