LOCUS       Exported                3488 bp ds-DNA     circular SYN 05-DEC-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   John Heap
  TITLE     Direct Submission
  JOURNAL   Exported Dec 5, 2017 from SnapGene 4.1.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      1..87
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(624..1169)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     CDS             1357..2547
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     terminator      2885..2979
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        3064..3094
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3102..3118
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             3138..3461
                     /codon_start=1
                     /gene="lacZ"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
ORIGIN
        1 atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc
       61 tttcgactga gcctttcgtt ttatttgatg cccgaaaggg cctcgtgata cgccaaaagt
      121 tggcccaggg cttcccggta tcaacaggga caccaggatt tatttattct gcgaagtgat
      181 cttccgtcac aggtatttat tcggcgcaaa gtgcgtcggg tgatgctgcc aacttactga
      241 tttagtgtat gatggtgttt ttgaggtgct ccagtggctt ctgtttctat cagctgtccc
      301 tcctgttcag ctactgacgg ggtggtgcgt aacggcaaaa gcaccgccgg acatcagcgc
      361 tagcggagtg tatactggct tactatgttg gcactgatga gggtgtcagt gaagtgcttc
      421 atgtggcagg agaaaaaagg ctgcaccggt gcgtcagcag aatatgtgat acaggatata
      481 ttccgcttcc tcgctcactg actcgctacg ctcggtcgtt cgactgcggc gagcggaaat
      541 ggcttacgaa cggggcggag atttcctgga agatgccagg aagatactta acagggaagt
      601 gagagggccg cggcaaagcc gtttttccat aggctccgcc cccctgacaa gcatcacgaa
      661 atctgacgct caaatcagtg gtggcgaaac ccgacaggac tataaagata ccaggcgttt
      721 ccccctggcg gctccctcgt gcgctctcct gttcctgcct ttcggtttac cggtgtcatt
      781 ccgctgttat ggccgcgttt gtctcattcc acgcctgaca ctcagttccg ggtaggcagt
      841 tcgctccaag ctggactgta tgcacgaacc ccccgttcag tccgaccgct gcgccttatc
      901 cggtaactat cgtcttgagt ccaacccgga aagacatgca aaagcaccac tggcagcagc
      961 cactggtaat tgatttagag gagttagtct tgaagtcatg cgccggttaa ggctaaactg
     1021 aaaggacaag ttttggtgac tgcgctcctc caagccagtt acctcggttc aaagagttgg
     1081 tagctcagag aaccttcgaa aaaccgccct gcaaggcggt tttttcgttt tcagagcaag
     1141 agattacgcg cagaccaaaa cgatctcaag aagatcatct tattaatcag ataaaatatt
     1201 tctagatttc agtgcaattt atctcttcaa atgtagcacc tgaagtcagc cccatacgat
     1261 ataagttgta attctcatgt ttgacagctt atcatcgata agctttaatg cggtagttta
     1321 tcacagttaa attgctaacg cagtcaggca ccgtgtatga aatctaacaa tgcgctcatc
     1381 gtcatcctcg gcaccgtcac cctggatgct gtaggcatag gcttggttat gccggtactg
     1441 ccgggcctct tgcgggatat cgtccattcc gacagcatcg ccagtcacta tggcgtgctg
     1501 ctagcgctat atgcgttgat gcaatttcta tgcgcacccg ttctcggagc actgtccgac
     1561 cgctttggcc gccgcccagt cctgctcgct tcgctacttg gagccactat cgactacgcg
     1621 atcatggcga ccacacccgt cctgtggatc ctctacgccg gacgcatcgt ggccggcatc
     1681 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat
     1741 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc
     1801 gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg
     1861 ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag
     1921 cgtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc
     1981 atgactatcg tcgccgcact tatgactgtt ttctttatca tgcaactcgt aggacaggtg
     2041 ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgatc
     2101 ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt cgtcactggt
     2161 cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc ggccgacgcg
     2221 ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc cattatgatt
     2281 cttctcgctt ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc caggcaggta
     2341 gatgacgacc atcagggaca gcttcaagga tcgctcgcgg ctcttaccag cctaacttcg
     2401 atcattggac cgctgatcgt cacggcgatt tatgccgcct cggcgagcac atggaacggg
     2461 ttggcatgga ttgtaggcgc cgccctatac cttgtctgcc tccccgcgtt gcgtcgcggt
     2521 gcatggagcc gggccacctc gacctgaatg gaagccggcg gcacctcgct aacggattca
     2581 ccactccaag aattggagcc aatcaattct tgcggagaac tgtgaatgcg caaaccaacc
     2641 cttggcagaa catatccatc gcgtccgcca tctccagcag ccgcacgcgg cgcatctcgg
     2701 gcagcgttgg gtcctggcca cgggtgcgca tgatcgtgct cctgtcgttg aggacccggc
     2761 taggctggcg gggttgcctt actggttagc agaatgaatc accgatacgc gagcgaacgt
     2821 gaagcgactg ctgctgcaaa acgtctgcga cctgagcgcc gcagccggac gaccgagcgc
     2881 cttggactcc tgttgataga tccagtaatg acctcagaac tccatctgga tttgttcaga
     2941 acgctcggtt gccgccgggc gttttttatt ggtgagaatg gtctcaggag cagagaagag
     3001 ccagtagggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     3061 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     3121 tcacacagga aacagctatg accatgatta cgccaagctt gcatgcctgc aggtcgactc
     3181 tagaggatcc ccgggtaccg agctcgaatt cactggccgt cgttttacaa cgtcgtgact
     3241 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     3301 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     3361 gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     3421 tatggtgcac tctcagtaca atctgctctg atgccgcata gtttccgctc ttcaggaaat
     3481 gagagacc
//
