LOCUS Exported 3488 bp ds-DNA circular SYN 05-DEC-2017 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3488) AUTHORS John Heap TITLE Direct Submission JOURNAL Exported Dec 5, 2017 from SnapGene 4.1.1 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3488 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 1..87 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" rep_origin complement(624..1169) /direction=LEFT /label=p15A ori /note="Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin." CDS 1357..2547 /codon_start=1 /gene="tet" /product="tetracycline efflux protein" /label=TcR /note="confers resistance to tetracycline" /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST" terminator 2885..2979 /label=lambda t0 terminator /note="transcription terminator from phage lambda" promoter 3064..3094 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 3102..3118 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." CDS 3138..3461 /codon_start=1 /gene="lacZ" /product="LacZ-alpha fragment of beta-galactosidase" /label=lacZ-alpha /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS DAA" ORIGIN 1 atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc 61 tttcgactga gcctttcgtt ttatttgatg cccgaaaggg cctcgtgata cgccaaaagt 121 tggcccaggg cttcccggta tcaacaggga caccaggatt tatttattct gcgaagtgat 181 cttccgtcac aggtatttat tcggcgcaaa gtgcgtcggg tgatgctgcc aacttactga 241 tttagtgtat gatggtgttt ttgaggtgct ccagtggctt ctgtttctat cagctgtccc 301 tcctgttcag ctactgacgg ggtggtgcgt aacggcaaaa gcaccgccgg acatcagcgc 361 tagcggagtg tatactggct tactatgttg gcactgatga gggtgtcagt gaagtgcttc 421 atgtggcagg agaaaaaagg ctgcaccggt gcgtcagcag aatatgtgat acaggatata 481 ttccgcttcc tcgctcactg actcgctacg ctcggtcgtt cgactgcggc gagcggaaat 541 ggcttacgaa cggggcggag atttcctgga agatgccagg aagatactta acagggaagt 601 gagagggccg cggcaaagcc gtttttccat aggctccgcc cccctgacaa gcatcacgaa 661 atctgacgct caaatcagtg gtggcgaaac ccgacaggac tataaagata ccaggcgttt 721 ccccctggcg gctccctcgt gcgctctcct gttcctgcct ttcggtttac cggtgtcatt 781 ccgctgttat ggccgcgttt gtctcattcc acgcctgaca ctcagttccg ggtaggcagt 841 tcgctccaag ctggactgta tgcacgaacc ccccgttcag tccgaccgct gcgccttatc 901 cggtaactat cgtcttgagt ccaacccgga aagacatgca aaagcaccac tggcagcagc 961 cactggtaat tgatttagag gagttagtct tgaagtcatg cgccggttaa ggctaaactg 1021 aaaggacaag ttttggtgac tgcgctcctc caagccagtt acctcggttc aaagagttgg 1081 tagctcagag aaccttcgaa aaaccgccct gcaaggcggt tttttcgttt tcagagcaag 1141 agattacgcg cagaccaaaa cgatctcaag aagatcatct tattaatcag ataaaatatt 1201 tctagatttc agtgcaattt atctcttcaa atgtagcacc tgaagtcagc cccatacgat 1261 ataagttgta attctcatgt ttgacagctt atcatcgata agctttaatg cggtagttta 1321 tcacagttaa attgctaacg cagtcaggca ccgtgtatga aatctaacaa tgcgctcatc 1381 gtcatcctcg gcaccgtcac cctggatgct gtaggcatag gcttggttat gccggtactg 1441 ccgggcctct tgcgggatat cgtccattcc gacagcatcg ccagtcacta tggcgtgctg 1501 ctagcgctat atgcgttgat gcaatttcta tgcgcacccg ttctcggagc actgtccgac 1561 cgctttggcc gccgcccagt cctgctcgct tcgctacttg gagccactat cgactacgcg 1621 atcatggcga ccacacccgt cctgtggatc ctctacgccg gacgcatcgt ggccggcatc 1681 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat 1741 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc 1801 gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg 1861 ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag 1921 cgtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc 1981 atgactatcg tcgccgcact tatgactgtt ttctttatca tgcaactcgt aggacaggtg 2041 ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgatc 2101 ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt cgtcactggt 2161 cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc ggccgacgcg 2221 ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc cattatgatt 2281 cttctcgctt ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc caggcaggta 2341 gatgacgacc atcagggaca gcttcaagga tcgctcgcgg ctcttaccag cctaacttcg 2401 atcattggac cgctgatcgt cacggcgatt tatgccgcct cggcgagcac atggaacggg 2461 ttggcatgga ttgtaggcgc cgccctatac cttgtctgcc tccccgcgtt gcgtcgcggt 2521 gcatggagcc gggccacctc gacctgaatg gaagccggcg gcacctcgct aacggattca 2581 ccactccaag aattggagcc aatcaattct tgcggagaac tgtgaatgcg caaaccaacc 2641 cttggcagaa catatccatc gcgtccgcca tctccagcag ccgcacgcgg cgcatctcgg 2701 gcagcgttgg gtcctggcca cgggtgcgca tgatcgtgct cctgtcgttg aggacccggc 2761 taggctggcg gggttgcctt actggttagc agaatgaatc accgatacgc gagcgaacgt 2821 gaagcgactg ctgctgcaaa acgtctgcga cctgagcgcc gcagccggac gaccgagcgc 2881 cttggactcc tgttgataga tccagtaatg acctcagaac tccatctgga tttgttcaga 2941 acgctcggtt gccgccgggc gttttttatt ggtgagaatg gtctcagctt cagagaagag 3001 ccagtagggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca 3061 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt 3121 tcacacagga aacagctatg accatgatta cgccaagctt gcatgcctgc aggtcgactc 3181 tagaggatcc ccgggtaccg agctcgaatt cactggccgt cgttttacaa cgtcgtgact 3241 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct 3301 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg 3361 gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca 3421 tatggtgcac tctcagtaca atctgctctg atgccgcata gtttccgctc ttcaggacgc 3481 tagagacc //