LOCUS       Exported                3040 bp ds-DNA     circular SYN 05-DEC-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   John Heap
  TITLE     Direct Submission
  JOURNAL   Exported Dec 5, 2017 from SnapGene 4.1.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      1..87
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      437..982
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     CDS             1577..2392
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     terminator      2435..2529
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        2615..2645
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2653..2669
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             2689..3012
                     /codon_start=1
                     /gene="lacZ"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
ORIGIN
        1 atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc
       61 tttcgactga gcctttcgtt ttatttgatg cccgaaaggg cctcgtgata cgcaaatctg
      121 gagccggtga gcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      181 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      241 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
      301 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
      361 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagacccct
      421 taataagatg atcttcttga gatcgttttg gtctgcgcgt aatctcttgc tctgaaaacg
      481 aaaaaaccgc cttgcagggc ggtttttcga aggttctctg agctaccaac tctttgaacc
      541 gaggtaactg gcttggagga gcgcagtcac caaaacttgt cctttcagtt tagccttaac
      601 cggcgcatga cttcaagact aactcctcta aatcaattac cagtggctgc tgccagtggt
      661 gcttttgcat gtctttccgg gttggactca agacgatagt taccggataa ggcgcagcgg
      721 tcggactgaa cggggggttc gtgcatacag tccagcttgg agcgaactgc ctacccggaa
      781 ctgagtgtca ggcgtggaat gagacaaacg cggccataac agcggaatga caccggtaaa
      841 ccgaaaggca ggaacaggag agcgcacgag ggagccgcca gggggaaacg cctggtatct
      901 ttatagtcct gtcgggtttc gccaccactg atttgagcgt cagatttcgt gatgcttgtc
      961 aggggggcgg agcctatgga aaaacggctt tgccgcggcc ctctcacttc cctgttaagt
     1021 atcttcctgg catcttccag gaaatctccg ccccgttcgt aagccatttc cgctcgccgc
     1081 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaatatat cctgtatcac
     1141 atattctgct gacgcaccgg tgcagccttt tttctcctgc cacatgaagc acttcactga
     1201 caccctcatc agtgccaaca tagtaagcca gtatacactc cgctagcgct gaggtctgcc
     1261 tcgtgaagaa ggtgttgctg actcatacca ggcctgaatc gccccatcat ccagccagaa
     1321 agtgagggag ccacggttga tgagagcttt gttgtaggtg gaccagttgg tgattttgaa
     1381 cttttgcttt gccacggaac ggtctgcgtt gtcgggaaga tgcgtgatct gatccttcaa
     1441 ctcagcaaaa gttcgattta ttcaacaaag ccacgttgtg tctcaaaatc tctgatgtta
     1501 cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag
     1561 taatacaagg ggtgttatga gccatattca acgggaaacg tcttgctcga ggccgcgatt
     1621 aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca
     1681 atcaggtgcg acaatctatc gattgtatgg gaagcccgat gcgccagagt tgtttctgaa
     1741 acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct
     1801 gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg
     1861 gttactcacc actgcgatcc ccgggaaaac agcattccag gtattagaag aatatcctga
     1921 ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc
     1981 tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg
     2041 aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt
     2101 tgaacaagtc tggaaagaaa tgcataagct tttgccattc tcaccggatt cagtcgtcac
     2161 tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat
     2221 tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg
     2281 cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa
     2341 tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct aatcttgggg
     2401 gaaattagcc gcagccgaac gaccgagcgc cttggactcc tgttgataga tccagtaatg
     2461 acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt
     2521 ggtgagaatg aagacaatgc cggagagaga cccagtaggg cagtgagcgc aacgcaatta
     2581 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2641 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2701 acgccaagct tgcatgcctg caggtcgact ctagaggatc cccgggtacc gagctcgaat
     2761 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     2821 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     2881 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc
     2941 cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct
     3001 gatgccgcat agtttccggt ctcatactac taaagtcttc
//
