LOCUS Exported 3196 bp ds-DNA circular SYN 05-DEC-2017 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3196) AUTHORS John Heap TITLE Direct Submission JOURNAL Exported Dec 5, 2017 from SnapGene 4.1.1 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3196 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 1..87 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" CDS complement(588..1247) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" promoter complement(1248..1350) /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" rep_origin complement(1876..2421) /direction=LEFT /label=p15A ori /note="Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin." terminator 2613..2707 /label=lambda t0 terminator /note="transcription terminator from phage lambda" promoter 2782..2812 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2820..2836 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." CDS 2856..3179 /codon_start=1 /gene="lacZ" /product="LacZ-alpha fragment of beta-galactosidase" /label=lacZ-alpha /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS DAA" ORIGIN 1 atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc 61 tttcgactga gcctttcgtt ttatttgatg cccgaaaggg cctcgtgata cgcctttatc 121 atgcaactcg taggacaggt gccggcagcg cccaacagtc ccccggccac ggggcctgcc 181 accataccca cgccgaaaca agcgccctgc accattatgt tccggatctg catcgcagga 241 tgctgctggc taccctgtgg aacacctaca tctgtattaa cgaagcgcta accgttttta 301 tcaggctctg ggaggcagaa taaatgatca tatcgtcaat tattacctcc acggggagag 361 cctgagcaaa ctggcctcag gcatttgaga agcacacggt cacactgctt ccggtagtca 421 ataaaccggt aaaccagcaa tagacataag cggctattta acgaccctgc cctgaaccga 481 cgaccgggtc gaatttgctt tcgaatttct gccattcatc cgcttattat cacttattca 541 ggcgtagcac caggcgttta agggcaccaa taactgcctt aaaaaaatta cgccccgccc 601 tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca 661 cagacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa 721 tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca 781 aaactggtga aactcaccca gggattggct gagacgaaaa acatattctc aataaaccct 841 ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga 901 aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca 961 tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt 1021 gccatacgga attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga 1081 taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg 1141 gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc 1201 cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta 1261 gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg 1321 tgaaagttgg aacctcttac gtgccgatca acgtctcatt ttcgccaaaa gttggcccag 1381 ggcttcccgg tatcaacagg gacaccagga tttatttatt ctgcgaagtg atcttccgtc 1441 acaggtattt attcggcgca aagtgcgtcg ggtgatgctg ccaacttact gatttagtgt 1501 atgatggtgt ttttgaggtg ctccagtggc ttctgtttct atcagctgtc cctcctgttc 1561 agctactgac ggggtggtgc gtaacggcaa aagcaccgcc ggacatcagc gctagcggag 1621 tgtatactgg cttactatgt tggcactgat gagggtgtca gtgaagtgct tcatgtggca 1681 ggagaaaaaa ggctgcaccg gtgcgtcagc agaatatgtg atacaggata tattccgctt 1741 cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg gcgagcggaa atggcttacg 1801 aacggggcgg agatttcctg gaagatgcca ggaagatact taacagggaa gtgagagggc 1861 cgcggcaaag ccgtttttcc ataggctccg cccccctgac aagcatcacg aaatctgacg 1921 ctcaaatcag tggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 1981 cggctccctc gtgcgctctc ctgttcctgc ctttcggttt accggtgtca ttccgctgtt 2041 atggccgcgt ttgtctcatt ccacgcctga cactcagttc cgggtaggca gttcgctcca 2101 agctggactg tatgcacgaa ccccccgttc agtccgaccg ctgcgcctta tccggtaact 2161 atcgtcttga gtccaacccg gaaagacatg caaaagcacc actggcagca gccactggta 2221 attgatttag aggagttagt cttgaagtca tgcgccggtt aaggctaaac tgaaaggaca 2281 agttttggtg actgcgctcc tccaagccag ttacctcggt tcaaagagtt ggtagctcag 2341 agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt tttcagagca agagattacg 2401 cgcagaccaa aacgatctca agaagatcat cttattaatc agataaaata tttctagatt 2461 tcagtgcaat ttatctcttc aaatgtagca cctgaagtca gccccatacg atataagttg 2521 taattctcat gtttgacagc ttatcatcga taagctttaa tgcggtagtt tatcacagtt 2581 aaattgccgc agccgaacga ccgagcgcct tggactcctg ttgatagatc cagtaatgac 2641 ctcagaactc catctggatt tgttcagaac gctcggttgc cgccgggcgt tttttattgg 2701 tgagaattgc caagtcttcc agtagggcag tgagcgcaac gcaattaatg tgagttagct 2761 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat 2821 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc 2881 atgcctgcag gtcgactcta gaggatcccc gggtaccgag ctcgaattca ctggccgtcg 2941 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac 3001 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac 3061 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt 3121 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt 3181 ttccgaagac aacgag //