LOCUS Exported 3853 bp ds-DNA circular SYN 23-JUL-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM recombinant plasmid REFERENCE 1 (bases 1 to 3853) AUTHORS John Heap TITLE Direct Submission JOURNAL Exported Jul 23, 2018 from SnapGene Viewer 4.2.2 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3853 /organism="recombinant plasmid" /mol_type="other DNA" promoter 144..248 /gene="bla" /label=AmpR promoter CDS 249..1109 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 1280..1868 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" terminator 2026..2120 /label=lambda t0 terminator /note="transcription terminator from phage lambda" CDS 2136..3782 /codon_start=1 /label=crtI /translation="MTSALPRPAPSPYARRKTALVIGAGFGGLALGIRLQSLGFDTTIL ERLDGPGGRAYQKRTPDGYVFDMGPTVLTVPHFIEELFALERDRAGLDAPDYPPEVLSG ERVKEGVSGGPHTSRYVTLVPILPFYRIVFHDGTYFDYDGDPESTRRQIAELAPGDLAG YERFHADAEAIFRRGFLELGYTHFGDVPTMLRVVPDLLKLDAVRTLFSFTSKYFQSDKL RQVFSFETLLVGGNPLSVPAIYAMIHFVEKTWGIHYAMGGTGALVRGLVQKFEELGGAI RYGAGVDEVLVDGNLPGKRTARGVRLESGEELRADLVASNGDWANTYLKRVRPSARLVN SDLRVKAASESMSLLVVYFGFRGGDDLPLKHHNILLGPRYEALLSEIFGTKRLGEDFSQ YLHVPTLTDPALAPAGHHAAYTLVPVPHNGSGIDWDVEGPKLAEAALADIERRGLIPGL RERLTHFEFITPDYFAGTLDSYLGNAFGPEPRLVQSAFFRPHNRSEDLHNFYLVGAGAQ PGAGTPSVMMSAKMTARLIAEDFGIHADIRR" terminator join(3812..3853,1..45) /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" ORIGIN 1 aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt atttgatgcc cgaaagggcc 61 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag 121 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 181 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 241 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt 301 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt 361 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt 421 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg 481 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga 541 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa 601 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga 661 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa 721 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca 781 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta 841 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac 901 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc 961 gtgggtcccg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag 1021 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga 1081 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt 1141 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 1201 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 1261 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 1321 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 1381 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc 1441 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 1501 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 1561 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 1621 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 1681 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 1741 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 1801 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 1861 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 1921 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg 1981 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg ccttggactc ctgttgatag 2041 atccagtaat gacctcagaa ctccatctgg atttgttcag aacgctcggt tgccgccggg 2101 cgttttttat tggtgagaat ccagtgtgct cttcgatgac atcagctctc ccccgcccgg 2161 caccaagtcc gtacgcacgc cgtaaaacgg cgttggttat tggcgcaggt tttggtgggt 2221 tggccctggg cattcgtcta cagtcgttag gttttgatac aacaattttg gaacgtctgg 2281 atggtcctgg tggtcgcgcg tatcaaaaac gtaccccaga tggctatgtc tttgacatgg 2341 gtccgactgt gctgacggtg ccgcatttta tcgaagaact gtttgcgctt gaacgtgatc 2401 gtgccggcct ggatgccccc gattatcctc ctgaagtgtt gtcgggcgag cgcgttaagg 2461 aaggcgtttc tggtggcccg catacgagcc ggtatgtcac cttagtgccg attctgccct 2521 tttaccgcat tgtttttcac gatggcacgt attttgatta tgatggcgac cctgaaagta 2581 ctcggcggca gattgctgaa ttggcccctg gcgacttagc cgggtatgaa cgctttcatg 2641 ccgatgccga ggccatcttt cgtcggggct tcctggaact gggctacacg cactttggtg 2701 acgtcccgac gatgctgcgg gttgtacctg atctgctcaa actggacgcc gttcggaccc 2761 tgttctcctt tacgagtaag tactttcaga gcgacaaact gcgccaagtg ttctcttttg 2821 aaaccctttt ggtgggtggg aatcctctga gtgtgccggc gatctatgca atgattcact 2881 tcgttgaaaa gacttggggg atccactatg ccatgggcgg cacaggcgca ctggtgcgcg 2941 gcctagtcca aaaatttgag gagctgggtg gcgccattcg ttatggcgcc ggcgtcgatg 3001 aagtactggt ggatggcaat ctgcctggta aacgcacagc gcggggtgtg cgcctggaaa 3061 gcggcgaaga actgcgcgcc gacctggtgg cgtccaatgg cgattgggct aacacgtatc 3121 tgaaacgcgt ccggccatcg gcacgtctgg tcaactctga tttacgcgtg aaagccgcat 3181 ctgaaagtat gagcctgctc gtggtttatt tcgggtttcg cggcggtgat gacctgcccc 3241 tcaaacatca taatatttta ttaggcccac gctacgaggc tctgctgagc gaaatctttg 3301 gcacaaaacg gttgggcgaa gattttagcc agtacctgca cgtcccaacg ctcaccgatc 3361 cggctctggc acccgcgggt catcatgcgg cctatacact tgtcccggtg ccgcataatg 3421 gctcgggtat tgattgggac gtggaaggtc caaagcttgc cgaagcagcc ctggcagata 3481 tcgagcgccg cggtttgatt ccgggcctcc gtgaacggct cacacatttt gaatttatta 3541 cgccagatta tttcgcaggc actctcgatt cctatctggg gaacgcgttt ggtccggagc 3601 cgcgtctggt ccagtcggca tttttccgcc cgcacaaccg cagcgaggat ctccacaact 3661 tttacttagt cggggcgggc gcgcagccag gcgcaggcac accgagcgtt atgatgtccg 3721 cgaaaatgac agcgcgccta atcgctgaag atttcggtat ccatgctgat atccggcgct 3781 aatgaagagc gtaagacctc tagggcggcg gatttgtcct actcaggaga gcgttcaccg 3841 acaaacaaca gat //