LOCUS       Exported                4069 bp ds-DNA     circular SYN 23-JUL-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   John Heap
  TITLE     Direct Submission
  JOURNAL   Exported Jul 23, 2018 from SnapGene Viewer 4.2.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     promoter        144..248
                     /gene="bla"
                     /label=AmpR promoter
     CDS             249..1109
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1280..1868
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      2026..2120
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             2136..3998
                     /codon_start=1
                     /label=dxs
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVSR
                     SSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKGGL
                     HPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMAFEA
                     MNHAGDIRPDMLVILNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSGVPPI
                     KELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQFLHIM
                     TKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAAKDNKLM
                     AITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIYSTFLQRA
                     YDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMTPSDENECR
                     QMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAILNFGTLMPE
                     AAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAGSGVNEVLMAH
                     RKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     terminator      join(4028..4069,1..45)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt atttgatgcc cgaaagggcc
       61 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
      121 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
      181 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      241 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      301 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      361 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      421 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      481 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      541 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      601 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      661 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      721 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      781 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      841 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      901 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      961 gtgggtcccg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     1021 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     1081 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1141 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1201 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1261 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1321 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1381 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     1441 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1501 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1561 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1621 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1681 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1741 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1801 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1861 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1921 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1981 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg ccttggactc ctgttgatag
     2041 atccagtaat gacctcagaa ctccatctgg atttgttcag aacgctcggt tgccgccggg
     2101 cgttttttat tggtgagaat ccagtgtgct cttcgatgtc ttttgatatt gcgaaatatc
     2161 caaccctggc cctagttgac tcgactcagg aattacgcct gctgccgaaa gagagccttc
     2221 caaagctgtg cgatgagtta cgccgctacc tcctggattc tgttagtcgt agctccggcc
     2281 acttcgcatc gggactaggt accgtcgaac tgacggttgc actgcattat gtgtataaca
     2341 ccccgttcga tcaattaatt tgggacgtcg gtcatcaagc atatccgcat aaaattctga
     2401 ccggtcgtcg cgacaagatc ggcacaattc gtcaaaaagg tggattgcat cctttcccgt
     2461 ggcgcggcga gtccgaatac gatgtgttga gcgttggcca ttcgtcaact tctatcagcg
     2521 ctggtattgg tattgcggtc gccgcggaga aagaaggcaa aaatcggcgt acggtttgcg
     2581 tgatcgggga tggtgcaatc accgcaggca tggcattcga agcgatgaac catgcggggg
     2641 acattcgtcc ggatatgcta gtgattctga acgataacga aatgagtatt tccgagaacg
     2701 tgggggctct taataaccac ttagcgcagc tgctgagcgg taaactttat tctagcctgc
     2761 gcgaaggcgg taagaaagtg ttctcaggcg tccctccgat taaagagctc cttaagcgta
     2821 ctgaggaaca cattaagggt atggtggttc caggcaccct gttcgaagaa ctgggtttta
     2881 attacattgg tcctgtggac ggccatgacg tgttaggctt aattaccacg ttaaaaaaca
     2941 tgcgcgatct gaaaggacct cagtttctcc acatcatgac caagaaaggt cgcggctatg
     3001 aaccggccga gaaagatccg attaccttcc acgcagttcc gaaattcgac ccatcctccg
     3061 gctgtctgcc gaaaagcagc ggaggcctgc cgagctattc caaaatcttt ggtgactggc
     3121 tgtgcgaaac tgcggcaaaa gataacaaac tgatggccat caccccggca atgcgcgaag
     3181 gttcgggaat ggtggaattc tctcgcaagt tcccagaccg ttattttgac gttgctatcg
     3241 ccgagcaaca tgcagtaacc tttgcggctg gtctggcaat tggtggttat aagccaattg
     3301 tggccatcta ttcaacgttc ttacaacgtg catatgacca ggtgctacat gatgtggcca
     3361 ttcagaaact gccggtctta tttgccatcg atcgtgcggg gatcgttggg gcagatggtc
     3421 agacgcatca gggcgccttc gacctaagtt atctgcgctg tatcccagaa atggtgatta
     3481 tgacccctag cgatgagaat gaatgtcgtc agatgttata caccggctat cattacaacg
     3541 acggaccatc cgctgtgcgc tacccgcgcg gaaacgcggt gggcgttgaa ctgaccccgt
     3601 tagaaaaatt accgatcgga aaagggattg ttaaacgtcg cggagaaaaa ctcgctattc
     3661 tgaacttcgg cacacttatg cccgaagcgg ctaaagtagc ggaaagccta aacgcaacct
     3721 tggtggatat gcgcttcgta aagccactgg acgaagccct aatcttagag atggccgcgt
     3781 cacatgaggc gttagttacg gtagaggaaa acgctattat gggcggagcg ggtagtggtg
     3841 tgaacgaagt tctgatggct caccgcaaac cggtgccggt actgaacatc ggcctcccgg
     3901 attttttcat tccccaaggt actcaagagg aaatgcgcgc cgagctcggc ttagatgctg
     3961 caggaatgga agccaaaatt aaggcctggc ttgcataatg aagagcgtaa gacctctagg
     4021 gcggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagat
//
