LOCUS       Exported                8950 bp ds-DNA     circular SYN 26-NOV-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 26, 2018 from SnapGene Viewer 4.2.5
            http://www.snapgene.com
COMMENT     Ligation of : 24X-MA_1_2.txt.xdna [6814 nt] : (SphI[4388] / 
            SpeI[4379]) to 12X-601-MA_3.txt.xdna [2128 nt] : (XbaI[131] / 
            SphI[2263])
FEATURES             Location/Qualifiers
     source          1..8950
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     66..83
                     /label=M13-fwd
     misc_feature    137..2254
                     /label=12X601-MA1
     misc_feature    2261..4378
                     /label=12X601-MA-2
     misc_feature    4385..6501
                     /label=12X601-MA3
     misc_feature    6685..6704
                     /label=New Feature
     rep_origin      7808..8436
                     /direction=RIGHT
                     /label=ColE1 origin
     terminator      complement(8748..8775)
                     /label=rrnB T2
     terminator      complement(8907..8950)
                     /label=rrnB T1
ORIGIN
        1 ttgatgcctg gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac
       61 gacgttgtaa aacgacggcc agtcttaagc gtctccccct gaacctgacc ccggaccaag
      121 tggtggctat tctagataac tgctcttagg cacaggatgt atatatctga cacgtgcctg
      181 gagactaggg agtaatcccc ttggcggtta aaacgcgggg gacagcgcgt acgtgcgttt
      241 aagcggtgct agagctgtct acgaccaatt gagcggcctc ggcaccggga ttctccaggt
      301 actggagaat tccagttcca tcagaagcac aggatgtata tatctgacac gtgcctggag
      361 actagggagt aatccccttg gcggttaaaa cgcgggggac agcgcgtacg tgcgtttaag
      421 cggtgctaga gctgtctacg accaattgag cggcctcggc accgggattc tccaggtact
      481 ggagaattcg gacgacagtg tgagcacagg atgtatatat ctgacacgtg cctggagact
      541 agggagtaat ccccttggcg gttaaaacgc gggggacagc gcgtacgtgc gtttaagcgg
      601 tgctagagct gtctacgacc aattgagcgg cctcggcacc gggattctcc aggtactgga
      661 gaattcccag ggatccttga gcacaggatg tatatatctg acacgtgcct ggagactagg
      721 gagtaatccc cttggcggtt aaaacgcggg ggacagcgcg tacgtgcgtt taagcggtgc
      781 tagagctgtc tacgaccaat tgagcggcct cggcaccggg attctccagg tactggagaa
      841 ttctgtttac accgacggca caggatgtat atatctgaca cgtgcctgga gactagggag
      901 taatcccctt ggcggttaaa acgcggggga cagcgcgtac gtgcgtttaa gcggtgctag
      961 agctgtctac gaccaattga gcggcctcgg caccgggatt ctccaggtac tggagaattc
     1021 atgctaacga gatcgcacag gatgtatata tctgacacgt gcctggagac tagggagtaa
     1081 tccccttggc ggttaaaacg cggggggtcg accgtacgtg cgtttaagcg gtgctagagc
     1141 tgtctacgac caattgagcg gcctcggcac cgggattctc caggtactgg agaattccgg
     1201 tccgtttacc agcacaggat gtatatatct gacacgtgcc tggagactag ggagtaatcc
     1261 ccttggcggt taaaacgcgg gggacagcgc gtacgtgcgt ttaagcggtg ctagagctgt
     1321 ctacgaccaa ttgagcggcc tcggcaccgg gattctccag gtactggaga attcgaaaaa
     1381 gagcgcaagc acaggatgta tatatctgac acgtgcctgg agactaggga gtaatcccct
     1441 tggcggttaa aacgcggggg acagcgcgta cgtgcgttta agcggtgcta gagctgtcta
     1501 cgaccaattg agcggcctcg gcaccgggat tctccaggta ctggagaatt ctacgcatat
     1561 actatgcaca ggatgtatat atctgacacg tgcctggaga ctagggagta atccccttgg
     1621 cggttaaaac gcgggggaca gcgcgtacgt gcgtttaagc ggtgctagag ctgtctacga
     1681 ccaattgagc ggcctcggca ccgggattct ccaggtactg gagaattcgc tcaagcttga
     1741 tcgcacagga tgtatatatc tgacacgtgc ctggagacta gggagtaatc cccttggcgg
     1801 ttaaaacgcg ggggacagcg cgtacgtgcg tttaagcggt gctagagctg tctacgacca
     1861 attgagcggc ctcggcaccg ggattctcca ggtactggag aattcagccc acgcagacgg
     1921 cacaggatgt atatatctga cacgtgcctg gagactaggg agtaatcccc ttggcggtta
     1981 aaacgcgggg gacagcgcgt acgtgcgttt aagcggtgct agagctgtct acgaccaatt
     2041 gagcggcctc ggcaccggga ttctccaggt actggagaat tcgcgtgtaa tacactgcac
     2101 aggatgtata tatctgacac gtgcctggag actagggagt aatccccttg gcggttaaaa
     2161 cgcgggggac agcgcgtacg tgcgtttaag cggtgctaga gctgtctacg accaattgag
     2221 cggcctcggc accgggattc tccaggtaga attcactaga taactgctct taggcacagg
     2281 atgtatatat ctgacacgtg cctggagact agggagtaat ccccttggcg gttaaaacgc
     2341 gggggacagc gcgtacgtgc gtttaagcgg tgctagagct gtctacgacc aattgagcgg
     2401 cctcggcacc gggattctcc aggtactgga gaattccagt tccatcagaa gcacaggatg
     2461 tatatatctg acacgtgcct ggagactagg gagtaatccc cttggcggtt aaaacgcggg
     2521 ggacagcgcg tacgtgcgtt taagcggtgc tagagctgtc tacgaccaat tgagcggcct
     2581 cggcaccggg attctccagg tactggagaa ttcggacgac agtgtgagca caggatgtat
     2641 atatctgaca cgtgcctgga gactagggag taatcccctt ggcggttaaa acgcggggga
     2701 cagcgcgtac gtgcgtttaa gcggtgctag agctgtctac gaccaattga gcggcctcgg
     2761 caccgggatt ctccaggtac tggagaattc ccagggatcc ttgagcacag gatgtatata
     2821 tctgacacgt gcctggagac tagggagtaa tccccttggc ggttaaaacg cgggggacag
     2881 cgcgtacgtg cgtttaagcg gtgctagagc tgtctacgac caattgagcg gcctcggcac
     2941 cgggattctc caggtactgg agaattctgt ttacaccgac ggcacaggat gtatatatct
     3001 gacacgtgcc tggagactag ggagtaatcc ccttggcggt taaaacgcgg gggacagcgc
     3061 gtacgtgcgt ttaagcggtg ctagagctgt ctacgaccaa ttgagcggcc tcggcaccgg
     3121 gattctccag gttgcgtggg cggaatgcga attctatcgc acaggatgta tatatctgac
     3181 acgtgcctgg agactaggga gtaatcccct tggcggttaa aacgcggggg gtcgaccgta
     3241 cgtgcgttta agcggtgcta gagctgtcta cgaccaattg agcggcctcg gcaccgggat
     3301 tctccaggta ctggagaatt ccggtccgtt taccagcaca ggatgtatat atctgacacg
     3361 tgcctggaga ctagggagta atccccttgg cggttaaaac gcgggggaca gcgcgtacgt
     3421 gcgtttaagc ggtgctagag ctgtctacga ccaattgagc ggcctcggca ccgggattct
     3481 ccaggtactg gagaattcga aaaagagcgc aagcacagga tgtatatatc tgacacgtgc
     3541 ctggagacta gggagtaatc cccttggcgg ttaaaacgcg ggggacagcg cgtacgtgcg
     3601 tttaagcggt gctagagctg tctacgacca attgagcggc ctcggcaccg ggattctcca
     3661 ggtactggag aattctacgc atatactatg cacaggatgt atatatctga cacgtgcctg
     3721 gagactaggg agtaatcccc ttggcggtta aaacgcgggg gacagcgcgt acgtgcgttt
     3781 aagcggtgct agagctgtct acgaccaatt gagcggcctc ggcaccggga ttctccaggt
     3841 actggagaat tcgctcaagc ttgatcgcac aggatgtata tatctgacac gtgcctggag
     3901 actagggagt aatccccttg gcggttaaaa cgcgggggac agcgcgtacg tgcgtttaag
     3961 cggtgctaga gctgtctacg accaattgag cggcctcggc accgggattc tccaggtact
     4021 ggagaattca gcccacgcag acggcacagg atgtatatat ctgacacgtg cctggagact
     4081 agggagtaat ccccttggcg gttaaaacgc gggggacagc gcgtacgtgc gtttaagcgg
     4141 tgctagagct gtctacgacc aattgagcgg cctcggcacc gggattctcc aggtactgga
     4201 gaattcgcgt gtaatacact gcacaggatg tatatatctg acacgtgcct ggagactagg
     4261 gagtaatccc cttggcggtt aaaacgcggg ggacagcgcg tacgtgcgtt taagcggtgc
     4321 tagagctgtc tacgaccaat tgagcggcct cggcaccggg attctccagg tagaattcac
     4381 tagataactg ctcttaggca caggatgtat atatctgaca cgtgcctgga gactagggag
     4441 taatcccctt ggcggttaaa acgcggggga cagcgcgtac gtgcgtttaa gcggtgctag
     4501 agctgtctac gaccaattga gcggcctcgg caccgggatt ctccaggtac tggagaattc
     4561 cagttccatc agaagcacag gatgtatata tctgacacgt gcctggagac tagggagtaa
     4621 tccccttggc ggttaaaacg cgggggacag cgcgtacgtg cgtttaagcg gtgctagagc
     4681 tgtctacgac caattgagcg gcctcggcac cgggattctc caggtactgg agaattcgga
     4741 cgacagtgtg agcacaggat gtatatatct gacacgtgcc tggagactag ggagtaatcc
     4801 ccttggcggt taaaacgcgg gggacagcgc gtacgtgcgt ttaagcggtg ctagagctgt
     4861 ctacgaccaa ttgagcggcc tcggcaccgg gattctccag gtactggaga attcccaggg
     4921 atccttgagc acaggatgta tatatctgac acgtgcctgg agactaggga gtaatcccct
     4981 tggcggttaa aacgcggggg acagcgcgta cgtgcgttta agcggtgcta gagctgtcta
     5041 cgaccaattg agcggcctcg gcaccgggat tctccaggta ctggagaatt ctgtttacac
     5101 cgacggcaca ggatgtatat atctgacacg tgcctggaga ctagggagta atccccttgg
     5161 cggttaaaac gcgggggaca gcgcgtacgt gcgtttaagc ggtgctagag ctgtctacga
     5221 ccaattgagc ggcctcggca ccgggattct ccaggtactg gagaattcat gctaacgaga
     5281 tcgcacagga tgtatatatc tgacacgtgc ctggagacta gggagtaatc cccttggcgg
     5341 ttaaaacgcg gggggtcgac cgtacgtgcg tttaagcggt gctagagctg tctacgacca
     5401 attgagcggc ctcggcaccg ggattctcca ggtactggag aattccggtc cgtttaccag
     5461 cacaggatgt atatatctga cacgtgcctg gagactaggg agtaatcccc ttggcggtta
     5521 aaacgcgggg gacagcgcgt acgtgcgttt aagcggtgct agagctgtct acgaccaatt
     5581 gagcggcctc ggcaccggga ttctccaggt tgcgtgggcg ggaaagaatt cgctagcaca
     5641 ggatgtatat atctgacacg tgcctggaga ctagggagta atccccttgg cggttaaaac
     5701 gcgggggaca gcgcgtacgt gcgtttaagc ggtgctagag ctgtctacga ccaattgagc
     5761 ggcctcggca ccgggattct ccaggtactg gagaattcta cgcatatact atgcacagga
     5821 tgtatatatc tgacacgtgc ctggagacta gggagtaatc cccttggcgg ttaaaacgcg
     5881 ggggacagcg cgtacgtgcg tttaagcggt gctagagctg tctacgacca attgagcggc
     5941 ctcggcaccg ggattctcca ggtactggag aattcgctca agcttgatcg cacaggatgt
     6001 atatatctga cacgtgcctg gagactaggg agtaatcccc ttggcggtta aaacgcgggg
     6061 gacagcgcgt acgtgcgttt aagcggtgct agagctgtct acgaccaatt gagcggcctc
     6121 ggcaccggga ttctccaggt actggagaat tcagcccacg cagacggcac aggatgtata
     6181 tatctgacac gtgcctggag actagggagt aatccccttg gcggttaaaa cgcgggggac
     6241 agcgcgtacg tgcgtttaag cggtgctaga gctgtctacg accaattgag cggcctcggc
     6301 accgggattc tccaggtact ggagaattcg cgtgtaatac actgcacagg atgtatatat
     6361 ctgacacgtg cctggagact agggagtaat ccccttggcg gttaaaacgc gggggacagc
     6421 gcgtacgtgc gtttaagcgg tgctagagct gtctacgacc aattgagcgg cctcggcacc
     6481 gggattctcc aggtagaatt cactagtgcg gcatgcggcg gcaagcaagc gctcgaaacg
     6541 gtgcagcggc tgttgccggt gctgtgccag gaccatggcc gagacgtcta gaccagccag
     6601 gacagaaatg cctcgacttc gctgctaccc aaggttgccg ggtgacgcac accgtggaaa
     6661 cggatgaagg cacgaaccca gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag
     6721 tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc
     6781 gcagtggcgg ttttcatggc ttgttatgac tgtttttttg gggtacagtc tatgcctcgg
     6841 gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac
     6901 gatgttacgc agcagggcag tcgccctaaa acaaagttaa acattatgag ggaagcggtg
     6961 atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa
     7021 ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac
     7081 agtgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct
     7141 ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct
     7201 gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc
     7261 gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc
     7321 acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg
     7381 gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg
     7441 ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat
     7501 gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag
     7561 gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt
     7621 gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag
     7681 ttggaagaat ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg caaataaccc
     7741 tcgagccacc catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca
     7801 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     7861 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     7921 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     7981 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     8041 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     8101 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     8161 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     8221 cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     8281 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     8341 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     8401 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     8461 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     8521 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     8581 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     8641 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     8701 gcaattaata cgcgtaccgc tagccaggaa gagtttgtag aaacgcaaaa aggccatccg
     8761 tcaggatggc cttctgctta gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc
     8821 accctccggg ccgttgcttc acaacgttca aatccgctcc cggcggattt gtcctactca
     8881 ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtcttcc gactgagcct
     8941 ttcgttttat
//
