LOCUS       Exported                9678 bp ds-DNA     circular SYN 10-AUG-2018
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9678)
  AUTHORS   locusliu
  TITLE     Direct Submission
  JOURNAL   Exported Jan 4, 2019 from SnapGene 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9678
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     promoter        complement(699..776)
                     /gene="lacI"
                     /label=lacI promoter
                     /note="color: #ffffff; direction: LEFT"
     promoter        1085..1103
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff"
     protein_bind    1104..1128
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     RBS             1143..1165
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
                     /note="color: #a6acb3"
     CDS             1173..1205
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /note="color: #993366"
                     /translation="MASMTGGQQMG"
     CDS             1215..1241
                     /codon_start=1
                     /product="nuclear localization signal (Makkerh et al., 
                     1996)"
                     /label=NLS
                     /note="color: #993366"
                     /translation="PAAKKKKLD"
     CDS             1254..5357
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /note="color: #993366"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             3324..3326
                     /codon_start=1
                     /label=R691
                     /note="color: #993366"
                     /translation="R"
     CDS             5403..5423
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     CDS             5436..5483
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /note="color: #993366"
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5490..5507
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /note="color: #993366"
                     /translation="HHHHHH"
     terminator      5574..5621
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
                     /note="color: #ffffff"
     rep_origin      5658..6113
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        6140..6244
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff"
     CDS             6245..7105
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #993366"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7276..7864
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     misc_feature    8050..8192
                     /label=bom
                     /note="basis of mobility region from pBR322"
                     /note="color: #a6acb3"
     CDS             complement(8294..8485)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /note="color: #993366"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(9294..9678,1..698))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #993366"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 agcatttgca tggtttgttg aaaaccggac atggcactcc agtcgccttc ccgttccgct
       61 atcggctgaa tttgattgcg agtgagatat ttatgccagc cagccagacg cagacgcgcc
      121 gagacagaac ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga
      181 tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa taatactgtt gatgggtgtc
      241 tggtcagaga catcaagaaa taacgccgga acattagtgc aggcagcttc cacagcaatg
      301 gcatcctggt catccagcgg atagttaatg atcagcccac tgacgcgttg cgcgagaaga
      361 ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg
      421 ctggcaccca gttgatcggc gcgagattta atcgccgcga caatttgcga cggcgcgtgc
      481 agggccagac tggaggtggc aacgccaatc agcaacgact gtttgcccgc cagttgttgt
      541 gccacgcggt tgggaatgta attcagctcc gccatcgccg cttccacttt ttcccgcgtt
      601 ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa cggtctgata agagacaccg
      661 gcatactctg cgacatcgta taacgttact ggtttcacat tcaccaccct gaattgactc
      721 tcttccgggc gctatcatgc cataccgcga aaggttttgc gccattcgat ggtgtccggg
      781 atctcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag
      841 gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc
      901 cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg
      961 gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt
     1021 ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg
     1081 aaattaatac gactcactat aggggaattg tgagcggata acaattcccc tctagaaata
     1141 attttgttta actttaagaa ggagatatac atatggctag catgactggt ggacagcaaa
     1201 tgggtcgcgc cgctccggca gctaagaaaa agaaactgga tggcagcgtc gacatggata
     1261 agaaatactc aataggctta gatatcggca caaatagcgt cggatgggcg gtgatcactg
     1321 atgaatataa ggttccgtct aaaaagttca aggttctggg aaatacagac cgccacagta
     1381 tcaaaaaaaa tcttataggg gctcttttat ttgacagtgg agagacagcg gaagcgactc
     1441 gtctcaaacg gacagctcgt agaaggtata cacgtcggaa gaatcgtatt tgttatctac
     1501 aggagatttt ttcaaatgag atggcgaaag tagatgatag tttctttcat cgacttgaag
     1561 agtctttttt ggtggaagaa gacaagaagc atgaacgtca tcctattttt ggaaatatag
     1621 tagatgaagt tgcttatcat gagaaatatc caactatcta tcatctgcga aaaaaattgg
     1681 tagattctac tgataaagcg gatttgcgct taatctattt ggccttagcg catatgatta
     1741 agtttcgtgg tcattttttg attgagggag atttaaatcc tgataatagt gatgtggaca
     1801 aactatttat ccagttggta caaacctaca atcaattatt tgaagaaaac cctattaacg
     1861 caagtggagt agatgctaaa gcgattcttt ctgcacgatt gagtaaatca agacgattag
     1921 aaaatctcat tgctcagctc cccggtgaga agaaaaatgg cttatttggg aatctcattg
     1981 ctttgtcatt gggtttgacc cctaatttta aatcaaattt tgatttggca gaagatgcta
     2041 aattacagct ttcaaaagat acttacgatg atgatttaga taatttattg gcgcaaattg
     2101 gagatcaata tgctgatttg tttttggcag ctaagaattt atcagatgct attttacttt
     2161 cagatatcct aagagtaaat actgaaataa ctaaggctcc cctatcagct tcaatgatta
     2221 aacgctacga tgaacatcat caagacttga ctcttttaaa agctttagtt cgacaacaac
     2281 ttccagaaaa gtataaagaa atcttttttg atcaatcaaa aaacggatat gcaggttata
     2341 ttgatggggg agctagccaa gaagaatttt ataaatttat caaaccaatt ttagaaaaaa
     2401 tggatggtac tgaggaatta ttggtgaaac taaatcgtga agatttgctg cgcaagcaac
     2461 ggacctttga caacggctct attccccatc aaattcactt gggtgagctg catgctattt
     2521 tgagaagaca agaagacttt tatccatttt taaaagacaa tcgtgagaag attgaaaaaa
     2581 tcttgacttt tcgaattcct tattatgttg gtccattggc gcgtggcaat agtcgttttg
     2641 catggatgac tcggaagtct gaagaaacaa ttaccccatg gaattttgaa gaagttgtcg
     2701 ataaaggtgc ttcagctcaa tcatttattg aacgcatgac aaactttgat aaaaatcttc
     2761 caaatgaaaa agtactacca aaacatagtt tgctttatga gtattttacg gtttataacg
     2821 aattgacaaa ggtcaaatat gttactgaag gaatgcgaaa accagcattt ctttcaggtg
     2881 aacagaagaa agccattgtt gatttactct tcaaaacaaa tcgaaaagta accgttaagc
     2941 aattaaaaga agattatttc aaaaaaatag aatgttttga tagtgttgaa atttcaggag
     3001 ttgaagatag atttaatgct tcattaggta cctaccatga tttgctaaaa attattaaag
     3061 ataaagattt tttggataat gaagaaaatg aagatatctt agaggatatt gttttaacat
     3121 tgaccttatt tgaagatagg gagatgattg aggaaagact taaaacatat gctcacctct
     3181 ttgatgataa ggtgatgaaa cagcttaaac gtcgccgtta tactggttgg ggacgtttgt
     3241 ctcgaaaatt gattaatggt attagggata agcaatctgg caaaacaata ttagattttt
     3301 tgaaatcaga tggttttgcc aatcgcaatt ttatgcagct gatccatgat gatagtttga
     3361 catttaaaga agacattcaa aaagcacaag tgtctggaca aggcgatagt ttacatgaac
     3421 atattgcaaa tttagctggt agccctgcta ttaaaaaagg tattttacag actgtaaaag
     3481 ttgttgatga attggtcaaa gtaatggggc ggcataagcc agaaaatatc gttattgaaa
     3541 tggcacgtga aaatcagaca actcaaaagg gccagaaaaa ttcgcgagag cgtatgaaac
     3601 gaatcgaaga aggtatcaaa gaattaggaa gtcagattct taaagagcat cctgttgaaa
     3661 atactcaatt gcaaaatgaa aagctctatc tctattatct ccaaaatgga agagacatgt
     3721 atgtggacca agaattagat attaatcgtt taagtgatta tgatgtcgat cacattgttc
     3781 cacaaagttt ccttaaagac gattcaatag acaataaggt cttaacgcgt tctgataaaa
     3841 atcgtggtaa atcggataac gttccaagtg aagaagtagt caaaaagatg aaaaactatt
     3901 ggagacaact tctaaacgcc aagttaatca ctcaacgtaa gtttgataat ttaacgaaag
     3961 ctgaacgtgg aggtttgagt gaacttgata aagctggttt tatcaaacgc caattggttg
     4021 aaactcgcca aatcactaag catgtggcac aaattttgga tagtcgcatg aatactaaat
     4081 acgatgaaaa tgataaactt attcgagagg ttaaagtgat taccttaaaa tctaaattag
     4141 tttctgactt ccgaaaagat ttccaattct ataaagtacg tgagattaac aattaccatc
     4201 atgcccatga tgcgtatcta aatgccgtcg ttggaactgc tttgattaag aaatatccaa
     4261 aacttgaatc ggagtttgtc tatggtgatt ataaagttta tgatgttcgt aaaatgattg
     4321 ctaagtctga gcaagaaata ggcaaagcaa ccgcaaaata tttcttttac tctaatatca
     4381 tgaacttctt caaaacagaa attacacttg caaatggaga gattcgcaaa cgccctctaa
     4441 tcgaaactaa tggggaaact ggagaaattg tctgggataa agggcgagat tttgccacag
     4501 tgcgcaaagt attgtccatg ccccaagtca atattgtcaa gaaaacagaa gtacagacag
     4561 gcggattctc caaggagtca attttaccaa aaagaaattc ggacaagctt attgctcgta
     4621 aaaaagactg ggatccaaaa aaatatggtg gttttgatag tccaacggta gcttattcag
     4681 tcctagtggt tgctaaggtg gaaaaaggga aatcgaagaa gttaaaatcc gttaaagagt
     4741 tactagggat cacaattatg gaaagaagtt cctttgaaaa aaatccgatt gactttttag
     4801 aagctaaagg atataaggaa gttaaaaaag acttaatcat taaactacct aaatatagtc
     4861 tttttgagtt agaaaacggt cgtaaacgga tgctggctag tgccggagaa ttacaaaaag
     4921 gaaatgagct ggctctgcca agcaaatatg tgaatttttt atatttagct agtcattatg
     4981 aaaagttgaa gggtagtcca gaagataacg aacaaaaaca attgtttgtg gagcagcata
     5041 agcattattt agatgagatt attgagcaaa tcagtgaatt ttctaagcgt gttattttag
     5101 cagatgccaa tttagataaa gttcttagtg catataacaa acatagagac aaaccaatac
     5161 gtgaacaagc agaaaatatt attcatttat ttacgttgac gaatcttgga gctcccgctg
     5221 cttttaaata ttttgataca acaattgatc gtaaacgata tacgtctaca aaagaagttt
     5281 tagatgccac tcttatccat caatccatca ctggtcttta tgaaacacgc attgatttga
     5341 gtcagctagg aggtgacacc ggtggtggtc ccgggggtgg tgcggccgca ggcagcggaa
     5401 gccctaagaa aaaacgaaaa gttggcagcg gaagcaaaag gccggcggcc acgaaaaagg
     5461 ccggccaggc aaaaaagaaa aagctcgagc accaccacca ccaccactga gatccggctg
     5521 ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat
     5581 aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat
     5641 ccggattggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     5701 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     5761 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     5821 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     5881 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     5941 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     6001 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     6061 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa tttcaggtgg
     6121 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     6181 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     6241 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     6301 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     6361 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     6421 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     6481 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     6541 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     6601 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     6661 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     6721 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     6781 gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     6841 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     6901 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     6961 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     7021 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     7081 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     7141 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     7201 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     7261 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     7321 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     7381 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     7441 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     7501 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     7561 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     7621 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     7681 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     7741 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     7801 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     7861 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     7921 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     7981 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     8041 ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     8101 atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc
     8161 cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg
     8221 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     8281 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt
     8341 aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca
     8401 gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa
     8461 gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg
     8521 gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac
     8581 atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc
     8641 agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac
     8701 agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact
     8761 tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg
     8821 tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct
     8881 gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca
     8941 tgcgcacccg tggggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg
     9001 tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg
     9061 acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg
     9121 ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga
     9181 tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg
     9241 gtcgagatcc cggtgcctaa tgagtgagct aacttacatt aattgcgttg cgctcactgc
     9301 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     9361 ggagaggcgg tttgcgtatt gggcgccagg gtggtttttc ttttcaccag tgagacgggc
     9421 aacagctgat tgcccttcac cgcctggccc tgagagagtt gcagcaagcg gtccacgctg
     9481 gtttgcccca gcaggcgaaa atcctgtttg atggtggtta acggcgggat ataacatgag
     9541 ctgtcttcgg tatcgtcgta tcccactacc gagatatccg caccaacgcg cagcccggac
     9601 tcggtaatgg cgcgcattgc gcccagcgcc atctgatcgt tggcaaccag catcgcagtg
     9661 ggaacgatgc cctcattc
//
