LOCUS       pAAV_EF1a_F14FRTFl        7041 bp ds-DNA   linear       22-MAY-2015
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    3319..3927
                     /label=WPRE
                     /ApEinfo_fwdcolor="#666666"
                     /ApEinfo_revcolor="green"
     misc_feature    1..141
                     /label=L-ITR
                     /ApEinfo_fwdcolor="#ffd404"
                     /ApEinfo_revcolor="green"
     misc_feature    4465..4605
                     /label=R-ITR
                     /ApEinfo_fwdcolor="#ffd00a"
                     /ApEinfo_revcolor="green"
     misc_feature    1544..1549
                     /label=EcoRI
                     /ApEinfo_fwdcolor="#ff0510"
                     /ApEinfo_revcolor="green"
     misc_feature    156..1419
                     /label=EF1a promoter
                     /ApEinfo_fwdcolor="#ff08b6"
                     /ApEinfo_revcolor="green"
     misc_feature    1420..1425
                     /label=KpnI
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
     Region          complement(3269..3302)
                     /label=FRT
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
     misc_feature    3305..3310
                     /label=EcoRI(1)
                     /ApEinfo_label="EcoRI"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
     Region          1510..1543
                     /label=FRT(1)
                     /ApEinfo_label="FRT"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
     misc_feature    complement(3185..3218)
                     /label=F14
                     /ApEinfo_fwdcolor="#ffdd07"
                     /ApEinfo_revcolor="#ffdd07"
     misc_feature    1426..1459
                     /label=F14(1)
                     /ApEinfo_label="F14"
                     /ApEinfo_fwdcolor="#ffdd07"
                     /ApEinfo_revcolor="#ffdd07"
     misc_feature    complement(3149..3154)
                     /label=BamHI
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="#ff0816"
     misc_feature    3163..3168
                     /label=SnaBI
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
     misc_feature    3177..3184
                     /label=PacI
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
     misc_feature    3169..3176
                     /label=AscI
                     /ApEinfo_fwdcolor="#ff0208"
                     /ApEinfo_revcolor="green"
     misc_feature    1555..1560
                     /label=BsrGI
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
     misc_feature    3157..3162
                     /label=NheI
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="green"
     exon            complement(1550..2266)
                     /label=EYFP (no start)
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
     gene            complement(2276..3148)
                     /note="GtACR2"
     misc_feature    3133..3148
                     /note="reverse primer"
     misc_feature    complement(2268..2275)
                     /note="NotI"
     source          1..7202
                     /dnas_title="pAAV-EF1a-FRT-FLEX-GtACR2-EYFP-WPRE
                     incompleted"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtaagct ttgcaaagat ggataaagtt
      181 ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat
      241 tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
      301 gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag
      361 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
      421 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc
      481 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat
      541 tacttccact ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      601 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      661 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      721 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      781 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      841 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      901 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      961 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     1021 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     1081 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     1141 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     1201 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1261 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1321 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1381 gtggttcaaa gtttttttct tccatttcag gtgtcgtgaG GTACCGAAGT TCCTATTCTA
     1441 TCAGAAGTAT AGGAACTTCg cagaatggta gctggattgt agctgctatt agcaatatga
     1501 aacctcttaG AAGTTCCTAT TCTCTAGAAA GTATAGGAAC TTCgaattcT TACTTGTACA
     1561 GCTCGTCCAT GCCGAGAGTG ATCCCGGCGG CGGTCACGAA CTCCAGCAGG ACCATGTGAT
     1621 CGCGCTTCTC GTTGGGGTCT TTGCTCAGGG CGGACTGGTA GCTCAGGTAG TGGTTGTCGG
     1681 GCAGCAGCAC GGGGCCGTCG CCGATGGGGG TGTTCTGCTG GTAGTGGTCG GCGAGCTGCA
     1741 CGCTGCCGTC CTCGATGTTG TGGCGGATCT TGAAGTTCAC CTTGATGCCG TTCTTCTGCT
     1801 TGTCGGCCAT GATATAGACG TTGTGGCTGT TGTAGTTGTA CTCCAGCTTG TGCCCCAGGA
     1861 TGTTGCCGTC CTCCTTGAAG TCGATGCCCT TCAGCTCGAT GCGGTTCACC AGGGTGTCGC
     1921 CCTCGAACTT CACCTCGGCG CGGGTCTTGT AGTTGCCGTC GTCCTTGAAG AAGATGGTGC
     1981 GCTCCTGGAC GTAGCCTTCG GGCATGGCGG ACTTGAAGAA GTCGTGCTGC TTCATGTGGT
     2041 CGGGGTAGCG GGCGAAGCAC TGCAGGCCGT AGCCGAAGGT GGTCACGAGG GTGGGCCAGG
     2101 GCACGGGCAG CTTGCCGGTG GTGCAGATGA ACTTCAGGGT CAGCTTGCCG TAGGTGGCAT
     2161 CGCCCTCGCC CTCGCCGGAC ACGCTGAACT TGTGGCCGTT TACGTCGCCG TCCAGCTCGA
     2221 CCAGGATGGG CACCACCCCG GTGAACAGCT CCTCGCCCTT GCTCACggcg gccgcCCTGC
     2281 CGAACATTCT GATGTTGACC TCGTCGTTGG GGGGGGCATT CTTCTCGTGG TTCAGTCCGA
     2341 AGGGGTCGTC GGCCTCAGGC TTCTCCTGGC CAGGGAGGCA AGCCGGGTCC CACTTTCCCT
     2401 CGAGCACTCC CCAGGCGGTG GACCACAGGA TCACGCCGTA GGTGTTCTTG CACAGGCCGT
     2461 CGGCGAGCAG ATACAGGATG GCGCTCACGT TCTCGTGGAT CACGCACACG CCGGTGGGGG
     2521 AAATCAGCCA CACGATGGGG AACATGGTCC AGGCGATGAA GAAGATCAGC CTCAGCATCT
     2581 TGATCCTCAC CACCACCTTC TGGGCCAGAG GGGTGCCCAC GGACTCGAAG CCGTACAGTC
     2641 CCACCTGGAA GATGGTGAAC ACCACGCTGT ATTCGAAGAC CAGGCAGGTA GCGCCGAAGA
     2701 AGAAGAACAG CCACTTCATG TACTCGGCGG GGGAGATGGT AGCGGTAATG CCCATCACCA
     2761 CCCTGATAAT GCTGGCGGCC TGGGCGATGG GGTTCAGAGG GATGCTCTTG TACTTCACCA
     2821 GAGCCATGTT GGAGATCTGG CCCAGCATAA TGGGGCATGT GCACAGCCAG CTGGCCATCC
     2881 TGGACCAGGG AAAGAACCTG CCGTCCCTCA TCTGCAGTGT GCCATTGCCG GTGCTGGCCA
     2941 GGGCGTATGT GATGGACTCC ACGAAGGGCA GGTACACGCT CTCCCAGGGC ACCTTGGGGG
     3001 CCCTGCTGAT CATGAAGATG GTCTGGCATC CGCTCACCAC GGCCCACACC AGCTGCAGCA
     3061 GGCTCACGAA GGTGCTGTCG ATCCTGCTCA TGTTGTAGCA CTCTGTGTGG GTGCTGGCCC
     3121 ACTCGCCGTA CACGACCTGG GAGGCCATGG ATCCCGGcta gcTACGTAgg cgcgccTTAA
     3181 TTAAGAAGTT CCTATACTTC TGATAGAATA GGAACTTCtt gccttaaccc agaaattatc
     3241 actgttattc tttagaatgg tgcaaagaGA AGTTCCTATA CTTTCTAGAG AATAGGAACT
     3301 TCCAGAATTC gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag
     3361 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     3421 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     3481 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     3541 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     3601 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     3661 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     3721 gaaatcatcg tcctttcctt ggctgctcgc ctatgttgcc acctggattc tgcgcgggac
     3781 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     3841 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     3901 ttgggccgcc tccccgcatc gataccgagc gctgctcgag agatctacgg gtggcatccc
     3961 tgtgacccct ccccagtgcc tctcctggcc ctggaagttg ccactccagt gcccaccagc
     4021 cttgtcctaa taaaattaag ttgcatcatt ttgtctgact aggtgtcctt ctataatatt
     4081 atggggtgga ggggggtggt atggagcaag gggcaagttg ggaagacaac ctgtagggcc
     4141 tgcggggtct attgggaacc aagctggagt gcagtggcac aatcttggct cactgcaatc
     4201 tccgcctcct gggttcaagc gattctcctg cctcagcctc ccgagttgtt gggattccag
     4261 gcatgcatga ccaggctcag ctaatttttg tttttttggt agagacgggg tttcaccata
     4321 ttggccaggc tggtctccaa ctcctaatct caggtgatct acccaccttg gcctcccaaa
     4381 ttgctgggat tacaggcgtg aaccactgct cccttccctg tccttctgat tttgtaggta
     4441 accacgtgcg gaccgagcgg ccgcaggaac ccctagtgat ggagttggcc actccctctc
     4501 tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg
     4561 cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt
     4621 attttctcct tacgcatctg tgcggtattt cacaccgcat acgtcaaagc aaccatagta
     4681 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     4741 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     4801 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     4861 tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc
     4921 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     4981 actcttgttc caaactggaa caacactcaa ccctatctcg ggctattctt ttgatttata
     5041 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     5101 cgcgaatttt aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg
     5161 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
     5221 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     5281 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
     5341 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     5401 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     5461 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     5521 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     5581 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     5641 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     5701 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     5761 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     5821 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     5881 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     5941 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     6001 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     6061 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     6121 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     6181 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     6241 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     6301 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     6361 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     6421 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     6481 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     6541 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     6601 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     6661 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     6721 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     6781 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     6841 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     6901 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     6961 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     7021 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     7081 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     7141 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     7201 gt
//

