LOCUS pAAV_EF1a_F14FRTFl 7041 bp ds-DNA circular 22-MAY-2015 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers misc_feature 3386..3994 /label=WPRE /ApEinfo_fwdcolor="#666666" /ApEinfo_revcolor="green" misc_feature 1..141 /label=L-ITR /ApEinfo_fwdcolor="#ffd404" /ApEinfo_revcolor="green" misc_feature 4532..4672 /label=R-ITR /ApEinfo_fwdcolor="#ffd00a" /ApEinfo_revcolor="green" misc_feature 1544..1549 /label=EcoRI /ApEinfo_fwdcolor="#ff0510" /ApEinfo_revcolor="green" misc_feature 156..1419 /label=EF1a promoter /ApEinfo_fwdcolor="#ff08b6" /ApEinfo_revcolor="green" misc_feature 1420..1425 /dnas_title="KpnI" /label=KpnI /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" Region complement(3336..3369) /label=FRT /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" misc_feature 3372..3377 /label=EcoRI(1) /ApEinfo_label="EcoRI" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" Region 1510..1543 /label=FRT(1) /ApEinfo_label="FRT" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" misc_feature complement(3252..3285) /label=F14 /ApEinfo_fwdcolor="#ffdd07" /ApEinfo_revcolor="#ffdd07" misc_feature 1426..1459 /label=F14(1) /ApEinfo_label="F14" /ApEinfo_fwdcolor="#ffdd07" /ApEinfo_revcolor="#ffdd07" misc_feature complement(3216..3221) /label=BamHI /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="#ff0816" misc_feature 3230..3235 /label=SnaBI /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="cyan" misc_feature 3244..3251 /label=PacI /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" misc_feature 3236..3243 /label=AscI /ApEinfo_fwdcolor="#ff0208" /ApEinfo_revcolor="green" misc_feature 1622..1627 /dnas_title="BsrGI" /label=BsrGI /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" misc_feature 3224..3229 /label=NheI /ApEinfo_fwdcolor="#ffff00" /ApEinfo_revcolor="green" gene complement(2343..3215) /note="GtACR2" misc_feature 3200..3215 /note="reverse primer" misc_feature complement(2335..2342) /note="NotI" PCR_primer complement(2343..2361) /pair="overlap for TS-EYFP" /primer="CATCAGAATGTTCGGCAGG" /current=0 PCR_primer 2343..2361 /primer="CCTGCCGAACATTCTGATG" /set="Set 2" /pair="Pair 1" /current=1 PCR_primer complement(3200..3216) /primer="CATGGCCTCCCAGGTCG" /set="Set 2" /pair="Pair 1" /current=1 PCR_product 2343..3216 /pair="Pair 1" /set="Set 2" /current=1 /score="1.000" PCR_frame 2343..3217 /note="Reading Frame" /set="Set 2" vector join(1..1555,1623..7269) /source="pAAV-EF1a-FRT-FLEX-GtACR2-EYFP-WPRE" /type="Custom cloned vector" misc_feature complement(1572..1619) /note="Kv4.2LL" /note="Amino acids 474-479" /note="from paper: An evolutionarily conserved dileucine motif in Shal K + channels mediates dendritic targeting, Rivera et al, nature neuroscience 2003" /translation="FETQHHHLLHCLEKTT" /note="Dendritic targeting domain from Kv4.2 rat" misc_feature 1561..1568 /note="PmeI" misc_feature 1569..1571 /note="stop" misc_feature 1555..1560 /note="BsrGI" exon complement(1620..2333) /dnas_title="EYFP (no start)" /label=EYFP (no start) /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" source 1..7269 /dnas_title="F-FLEX-ACR2-EYFP-KvD" ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 121 actccatcac taggggttcc tgcggccgca cgcgtaagct ttgcaaagat ggataaagtt 181 ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat 241 tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg 301 gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag 361 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc 421 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc 481 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat 541 tacttccact ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg 601 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg 661 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct 721 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg 781 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc 841 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga 901 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct 961 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca 1021 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg 1081 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg 1141 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat 1201 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg 1261 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa 1321 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca 1381 gtggttcaaa gtttttttct tccatttcag gtgtcgtgaG GTACCGAAGT TCCTATTCTA 1441 TCAGAAGTAT AGGAACTTCg cagaatggta gctggattgt agctgctatt agcaatatga 1501 aacctcttaG AAGTTCCTAT TCTCTAGAAA GTATAGGAAC TTCgaattcT TACTTGTACA 1561 gtttaaactc aCGTGGTTTT CTCCAGGCAG TGAagcAGGT GGTGGTGTTG TGTCTCGAAC 1621 TTGTACAGCT CGTCCATGCC GAGAGTGATC CCGGCGGCGG TCACGAACTC CAGCAGGACC 1681 ATGTGATCGC GCTTCTCGTT GGGGTCTTTG CTCAGGGCGG ACTGGTAGCT CAGGTAGTGG 1741 TTGTCGGGCA GCAGCACGGG GCCGTCGCCG ATGGGGGTGT TCTGCTGGTA GTGGTCGGCG 1801 AGCTGCACGC TGCCGTCCTC GATGTTGTGG CGGATCTTGA AGTTCACCTT GATGCCGTTC 1861 TTCTGCTTGT CGGCCATGAT ATAGACGTTG TGGCTGTTGT AGTTGTACTC CAGCTTGTGC 1921 CCCAGGATGT TGCCGTCCTC CTTGAAGTCG ATGCCCTTCA GCTCGATGCG GTTCACCAGG 1981 GTGTCGCCCT CGAACTTCAC CTCGGCGCGG GTCTTGTAGT TGCCGTCGTC CTTGAAGAAG 2041 ATGGTGCGCT CCTGGACGTA GCCTTCGGGC ATGGCGGACT TGAAGAAGTC GTGCTGCTTC 2101 ATGTGGTCGG GGTAGCGGGC GAAGCACTGC AGGCCGTAGC CGAAGGTGGT CACGAGGGTG 2161 GGCCAGGGCA CGGGCAGCTT GCCGGTGGTG CAGATGAACT TCAGGGTCAG CTTGCCGTAG 2221 GTGGCATCGC CCTCGCCCTC GCCGGACACG CTGAACTTGT GGCCGTTTAC GTCGCCGTCC 2281 AGCTCGACCA GGATGGGCAC CACCCCGGTG AACAGCTCCT CGCCCTTGCT CACggcggcc 2341 gcCCTGCCGA ACATTCTGAT GTTGACCTCG TCGTTGGGGG GGGCATTCTT CTCGTGGTTC 2401 AGTCCGAAGG GGTCGTCGGC CTCAGGCTTC TCCTGGCCAG GGAGGCAAGC CGGGTCCCAC 2461 TTTCCCTCGA GCACTCCCCA GGCGGTGGAC CACAGGATCA CGCCGTAGGT GTTCTTGCAC 2521 AGGCCGTCGG CGAGCAGATA CAGGATGGCG CTCACGTTCT CGTGGATCAC GCACACGCCG 2581 GTGGGGGAAA TCAGCCACAC GATGGGGAAC ATGGTCCAGG CGATGAAGAA GATCAGCCTC 2641 AGCATCTTGA TCCTCACCAC CACCTTCTGG GCCAGAGGGG TGCCCACGGA CTCGAAGCCG 2701 TACAGTCCCA CCTGGAAGAT GGTGAACACC ACGCTGTATT CGAAGACCAG GCAGGTAGCG 2761 CCGAAGAAGA AGAACAGCCA CTTCATGTAC TCGGCGGGGG AGATGGTAGC GGTAATGCCC 2821 ATCACCACCC TGATAATGCT GGCGGCCTGG GCGATGGGGT TCAGAGGGAT GCTCTTGTAC 2881 TTCACCAGAG CCATGTTGGA GATCTGGCCC AGCATAATGG GGCATGTGCA CAGCCAGCTG 2941 GCCATCCTGG ACCAGGGAAA GAACCTGCCG TCCCTCATCT GCAGTGTGCC ATTGCCGGTG 3001 CTGGCCAGGG CGTATGTGAT GGACTCCACG AAGGGCAGGT ACACGCTCTC CCAGGGCACC 3061 TTGGGGGCCC TGCTGATCAT GAAGATGGTC TGGCATCCGC TCACCACGGC CCACACCAGC 3121 TGCAGCAGGC TCACGAAGGT GCTGTCGATC CTGCTCATGT TGTAGCACTC TGTGTGGGTG 3181 CTGGCCCACT CGCCGTACAC GACCTGGGAG GCCATGGATC CCGGctagcT ACGTAggcgc 3241 gccTTAATTA AGAAGTTCCT ATACTTCTGA TAGAATAGGA ACTTCttgcc ttaacccaga 3301 aattatcact gttattcttt agaatggtgc aaagaGAAGT TCCTATACTT TCTAGAGAAT 3361 AGGAACTTCC AGAATTCgat atcaagctta tcgataatca acctctggat tacaaaattt 3421 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg 3481 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt 3541 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg 3601 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc 3661 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg 3721 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt 3781 tgtcggggaa atcatcgtcc tttccttggc tgctcgccta tgttgccacc tggattctgc 3841 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg 3901 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga 3961 tctccctttg ggccgcctcc ccgcatcgat accgagcgct gctcgagaga tctacgggtg 4021 gcatccctgt gacccctccc cagtgcctct cctggccctg gaagttgcca ctccagtgcc 4081 caccagcctt gtcctaataa aattaagttg catcattttg tctgactagg tgtccttcta 4141 taatattatg gggtggaggg gggtggtatg gagcaagggg caagttggga agacaacctg 4201 tagggcctgc ggggtctatt gggaaccaag ctggagtgca gtggcacaat cttggctcac 4261 tgcaatctcc gcctcctggg ttcaagcgat tctcctgcct cagcctcccg agttgttggg 4321 attccaggca tgcatgacca ggctcagcta atttttgttt ttttggtaga gacggggttt 4381 caccatattg gccaggctgg tctccaactc ctaatctcag gtgatctacc caccttggcc 4441 tcccaaattg ctgggattac aggcgtgaac cactgctccc ttccctgtcc ttctgatttt 4501 gtaggtaacc acgtgcggac cgagcggccg caggaacccc tagtgatgga gttggccact 4561 ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg 4621 ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg 4681 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg tcaaagcaac 4741 catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg 4801 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc 4861 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc 4921 gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta 4981 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta 5041 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcgggc tattcttttg 5101 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa 5161 aatttaacgc gaattttaac aaaatattaa cgtttacaat tttatggtgc actctcagta 5221 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg 5281 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg 5341 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc 5401 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag 5461 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 5521 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 5581 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt 5641 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt 5701 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt 5761 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg 5821 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga 5881 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa 5941 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga 6001 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa 6061 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca 6121 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta 6181 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac 6241 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc 6301 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag 6361 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga 6421 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt 6481 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 6541 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 6601 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 6661 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 6721 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc 6781 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 6841 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 6901 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 6961 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 7021 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 7081 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 7141 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 7201 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 7261 ctcacatgt //