LOCUS pAAV_EF1a_F14FRTFl 7041 bp ds-DNA circular 22-MAY-2015 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers misc_feature 3584..4192 /label=WPRE /ApEinfo_fwdcolor="#666666" /ApEinfo_revcolor="green" misc_feature 1..141 /label=L-ITR /ApEinfo_fwdcolor="#ffd404" /ApEinfo_revcolor="green" misc_feature 4730..4870 /label=R-ITR /ApEinfo_fwdcolor="#ffd00a" /ApEinfo_revcolor="green" misc_feature 1544..1549 /label=EcoRI /ApEinfo_fwdcolor="#ff0510" /ApEinfo_revcolor="green" misc_feature 156..1419 /label=EF1a promoter /ApEinfo_fwdcolor="#ff08b6" /ApEinfo_revcolor="green" misc_feature 1420..1425 /label=KpnI /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" Region complement(3534..3567) /label=FRT /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" misc_feature 3570..3575 /label=EcoRI(1) /ApEinfo_label="EcoRI" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" Region 1510..1543 /label=FRT(1) /ApEinfo_label="FRT" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" misc_feature complement(3450..3483) /label=F14 /ApEinfo_fwdcolor="#ffdd07" /ApEinfo_revcolor="#ffdd07" misc_feature 1426..1459 /label=F14(1) /ApEinfo_label="F14" /ApEinfo_fwdcolor="#ffdd07" /ApEinfo_revcolor="#ffdd07" misc_feature complement(3414..3419) /label=BamHI /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="#ff0816" misc_feature 3428..3433 /label=SnaBI /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="cyan" misc_feature 3442..3449 /label=PacI /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" misc_feature 3434..3441 /label=AscI /ApEinfo_fwdcolor="#ff0208" /ApEinfo_revcolor="green" misc_feature 1820..1825 /dnas_title="BsrGI" /label=BsrGI /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" misc_feature 3422..3427 /label=NheI /ApEinfo_fwdcolor="#ffff00" /ApEinfo_revcolor="green" exon complement(1818..2531) /dnas_title="EYFP (no start)" /label=EYFP (no start) /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" gene complement(2541..3413) /note="GtACR2" misc_feature complement(2533..2540) /note="NotI" vector join(1..1555,1821..7467) /source="pAAV-EF1a-FRT-FLEX-GtACR2-EYFP-WPRE" /type="Custom cloned vector" insert 1556..1820 /source="Kv2.1+TLCND PCR product" /type="Custom cloned insert" misc_feature complement(1572..1622) /dnas_title="TlcnC" /note="TLCND" /note="from paper: A Novel Phenylalanine-Based Targeting Signal Directs Telencephalin to Neuronal Dendrites, Mitsui et al" /translation="AESPADGEVFAIQLTSS" /note="from moust (but in paper they used rabbit)" /note="dendritic targeting domain" misc_feature 1561..1568 /note="AscI" misc_feature 1569..1571 /note="stop" exon complement(1623..1817) /dnas_title="Kv2.1C" /vntifkey="61" /label=Kv2.1 motif misc_feature 1555..1560 /note="BsrGI" misc_feature 1365..1388 /note="EF1a-F sequencing primer" misc_feature complement(2024..2047) /note="EGFP/EYFP-F488 sequencing primer" source 1..7467 /dnas_title="#202 pAAV-EF1a-FRT-FLEX-GtACR2-EYFP-Kv2.1C-TlcnC" ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 121 actccatcac taggggttcc tgcggccgca cgcgtaagct ttgcaaagat ggataaagtt 181 ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat 241 tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg 301 gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag 361 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc 421 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc 481 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat 541 tacttccact ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg 601 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg 661 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct 721 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg 781 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc 841 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga 901 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct 961 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca 1021 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg 1081 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg 1141 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat 1201 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg 1261 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa 1321 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca 1381 gtggttcaaa gtttttttct tccatttcag gtgtcgtgaG GTACCGAAGT TCCTATTCTA 1441 TCAGAAGTAT AGGAACTTCg cagaatggta gctggattgt agctgctatt agcaatatga 1501 aacctcttaG AAGTTCCTAT TCTCTAGAAA GTATAGGAAC TTCgaattcT TACTTGTACA 1561 ggcgcgcctc aGGAAGATGT CAGCTGGATA GCGAAAACCT CGCCATCTGC AGGTGACTCT 1621 GCaaaccttg tagcttcggg gaagtcggtg gcgcaggaaa tgaagctatc gatactagac 1681 atgcttctca tgtcaatcac gccctcggtc ctagctggca gaggagccac tggagatggc 1741 atggagctca tttccagttc ctcagggggt ttactctgag gggccatctc cttagtgttc 1801 aggatgggct gagactgctt GTACAGCTCG TCCATGCCGA GAGTGATCCC GGCGGCGGTC 1861 ACGAACTCCA GCAGGACCAT GTGATCGCGC TTCTCGTTGG GGTCTTTGCT CAGGGCGGAC 1921 TGGTAGCTCA GGTAGTGGTT GTCGGGCAGC AGCACGGGGC CGTCGCCGAT GGGGGTGTTC 1981 TGCTGGTAGT GGTCGGCGAG CTGCACGCTG CCGTCCTCGA TGTTGTGGCG GATCTTGAAG 2041 TTCACCTTGA TGCCGTTCTT CTGCTTGTCG GCCATGATAT AGACGTTGTG GCTGTTGTAG 2101 TTGTACTCCA GCTTGTGCCC CAGGATGTTG CCGTCCTCCT TGAAGTCGAT GCCCTTCAGC 2161 TCGATGCGGT TCACCAGGGT GTCGCCCTCG AACTTCACCT CGGCGCGGGT CTTGTAGTTG 2221 CCGTCGTCCT TGAAGAAGAT GGTGCGCTCC TGGACGTAGC CTTCGGGCAT GGCGGACTTG 2281 AAGAAGTCGT GCTGCTTCAT GTGGTCGGGG TAGCGGGCGA AGCACTGCAG GCCGTAGCCG 2341 AAGGTGGTCA CGAGGGTGGG CCAGGGCACG GGCAGCTTGC CGGTGGTGCA GATGAACTTC 2401 AGGGTCAGCT TGCCGTAGGT GGCATCGCCC TCGCCCTCGC CGGACACGCT GAACTTGTGG 2461 CCGTTTACGT CGCCGTCCAG CTCGACCAGG ATGGGCACCA CCCCGGTGAA CAGCTCCTCG 2521 CCCTTGCTCA Cggcggccgc CCTGCCGAAC ATTCTGATGT TGACCTCGTC GTTGGGGGGG 2581 GCATTCTTCT CGTGGTTCAG TCCGAAGGGG TCGTCGGCCT CAGGCTTCTC CTGGCCAGGG 2641 AGGCAAGCCG GGTCCCACTT TCCCTCGAGC ACTCCCCAGG CGGTGGACCA CAGGATCACG 2701 CCGTAGGTGT TCTTGCACAG GCCGTCGGCG AGCAGATACA GGATGGCGCT CACGTTCTCG 2761 TGGATCACGC ACACGCCGGT GGGGGAAATC AGCCACACGA TGGGGAACAT GGTCCAGGCG 2821 ATGAAGAAGA TCAGCCTCAG CATCTTGATC CTCACCACCA CCTTCTGGGC CAGAGGGGTG 2881 CCCACGGACT CGAAGCCGTA CAGTCCCACC TGGAAGATGG TGAACACCAC GCTGTATTCG 2941 AAGACCAGGC AGGTAGCGCC GAAGAAGAAG AACAGCCACT TCATGTACTC GGCGGGGGAG 3001 ATGGTAGCGG TAATGCCCAT CACCACCCTG ATAATGCTGG CGGCCTGGGC GATGGGGTTC 3061 AGAGGGATGC TCTTGTACTT CACCAGAGCC ATGTTGGAGA TCTGGCCCAG CATAATGGGG 3121 CATGTGCACA GCCAGCTGGC CATCCTGGAC CAGGGAAAGA ACCTGCCGTC CCTCATCTGC 3181 AGTGTGCCAT TGCCGGTGCT GGCCAGGGCG TATGTGATGG ACTCCACGAA GGGCAGGTAC 3241 ACGCTCTCCC AGGGCACCTT GGGGGCCCTG CTGATCATGA AGATGGTCTG GCATCCGCTC 3301 ACCACGGCCC ACACCAGCTG CAGCAGGCTC ACGAAGGTGC TGTCGATCCT GCTCATGTTG 3361 TAGCACTCTG TGTGGGTGCT GGCCCACTCG CCGTACACGA CCTGGGAGGC CATGGATCCC 3421 GGctagcTAC GTAggcgcgc cTTAATTAAG AAGTTCCTAT ACTTCTGATA GAATAGGAAC 3481 TTCttgcctt aacccagaaa ttatcactgt tattctttag aatggtgcaa agaGAAGTTC 3541 CTATACTTTC TAGAGAATAG GAACTTCCAG AATTCgatat caagcttatc gataatcaac 3601 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta 3661 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt 3721 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg 3781 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg 3841 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca 3901 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca 3961 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctatg 4021 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag 4081 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc 4141 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgagcgctgc 4201 tcgagagatc tacgggtggc atccctgtga cccctcccca gtgcctctcc tggccctgga 4261 agttgccact ccagtgccca ccagccttgt cctaataaaa ttaagttgca tcattttgtc 4321 tgactaggtg tccttctata atattatggg gtggaggggg gtggtatgga gcaaggggca 4381 agttgggaag acaacctgta gggcctgcgg ggtctattgg gaaccaagct ggagtgcagt 4441 ggcacaatct tggctcactg caatctccgc ctcctgggtt caagcgattc tcctgcctca 4501 gcctcccgag ttgttgggat tccaggcatg catgaccagg ctcagctaat ttttgttttt 4561 ttggtagaga cggggtttca ccatattggc caggctggtc tccaactcct aatctcaggt 4621 gatctaccca ccttggcctc ccaaattgct gggattacag gcgtgaacca ctgctccctt 4681 ccctgtcctt ctgattttgt aggtaaccac gtgcggaccg agcggccgca ggaaccccta 4741 gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca 4801 aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc 4861 tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac 4921 cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg 4981 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg 5041 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg 5101 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt 5161 tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt 5221 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta 5281 tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa 5341 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt 5401 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc 5461 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac 5521 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac 5581 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa 5641 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt 5701 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc 5761 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc 5821 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa 5881 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg 5941 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag 6001 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc 6061 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta 6121 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg 6181 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca 6241 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac 6301 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat 6361 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg 6421 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata 6481 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta 6541 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa 6601 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag 6661 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg 6721 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact 6781 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg 6841 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc 6901 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata 6961 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta 7021 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc 7081 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg 7141 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac 7201 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg 7261 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt 7321 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct 7381 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg 7441 ccttttgctg gccttttgct cacatgt //