LOCUS       114404_AICSDP-36        4717 bp    DNA     linear   UNA 19-DEC-2018
DEFINITION  A new nucleotide sequence entered manually.
ACCESSION   urn.local.amandah.64-9vz64la
VERSION     urn.local.amandah.64-9vz64la
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     unsure          1..808
                     /created_by="User"
                     /modified_by="User"
                     /label="AAVS1 5-prime Homology Arm"
     misc_RNA        786..805
                     /created_by="User"
                     /modified_by="User"
                     /label="crRNA2 Binding Site"
     misc_RNA        805..808
                     /created_by="User"
                     /label="crRNA1 Binding Site"
     misc_feature    808
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     modified_base   808
                     /created_by="User"
                     /modified_by="User"
                     /label="G to C"
     promoter        1875..2381
                     /created_by="User"
                     /modified_by="User"
                     /label="hPGK_promoter"
     ORF             2403..3119
                     /created_by="User"
                     /modified_by="User"
                     /label="mEGFP"
     misc_feature    2405
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     polyA_site      3132..3658
                     /created_by="User"
                     /label="Beta_globin_polyA_terminator"
     modified_base   3880
                     /created_by="User"
                     /modified_by="User"
                     /label="C to T"
     unsure          3881..4717
                     /created_by="User"
                     /modified_by="User"
                     /label="AAVS1 3-prime Homology Arm"
     misc_RNA        3881..3894
                     /created_by="User"
                     /label="crRNA1 Binding Site"
ORIGIN      
        1 ccctttgctt tctctgacca gcattctctc ccctgggcct gtgccgcttt ctgtctgcag
       61 cttgtggcct gggtcacctc tacggctggc ccagatcctt ccctgccgcc tccttcaggt
      121 tccgtcttcc tccactccct cttccccttg ctctctgctg tgttgctgcc caaggatgct
      181 ctttccggag cacttccttc tcggcgctgc accacgtgat gtcctctgag cggatcctcc
      241 ccgtgtctgg gtcctctccg ggcatctctc ctccctcacc caaccccatg ccgtcttcac
      301 tcgctgggtt cccttttcct tctccttctg gggcctgtgc catctctcgt ttcttaggat
      361 ggccttctcc gacggatgtc tcccttgcgt cccgcctccc cttcttgtag gcctgcatca
      421 tcaccgtttt tctggacaac cccaaagtac cccgtctccc tggctttagc cacctctcca
      481 tcctcttgct ttctttgcct ggacaccccg ttctcctgtg gattcgggtc acctctcact
      541 cctttcattt gggcagctcc cctacccccc ttacctctct agtctgtgct agctcttcca
      601 gccccctgtc atggcatctt ccaggggtcc gagagctcag ctagtcttct tcctccaacc
      661 cgggccccta tgtccacttc aggacagcat gtttgctgcc tccagggatc ctgtgtcccc
      721 gagctgggac caccttatat tcccagggcc ggttaatgtg gctctggttc tgggtacttt
      781 tatctgtccc ctccacccca cagtgggcca agcttctgac ctcttctctt cctcccacag
      841 ggcctcgaga gatctggcag cggagagggc agaggaagtc ttctaacatg cggtgacgtg
      901 gaggagaatc ccggccctag gctcgagatg accgagtaca agcccacggt gcgcctcgcc
      961 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     1021 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     1081 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     1141 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     1201 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     1261 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     1321 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     1381 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
     1441 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     1501 tgcatgaccc gcaagcccgg tgcctgatct agagggcccg tttaaacccg ctgatcagcc
     1561 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     1621 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     1681 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     1741 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggg tctcgacatt
     1801 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
     1861 tggagttccg cgttttgggg ttgcgccttt tccaaggcag ccctgggttt gcgcagggac
     1921 gcggctgctc tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg tctcgcacat
     1981 tcttcacgtc cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg ggccccccgg
     2041 cgacgcttcc tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga
     2101 cgtgacaaac ggaagccgca cgtctcacta gtaccctcgc agacggacag cgccagggag
     2161 caatggcagc gcgccgaccg cgatgggctg tggccaatag cggctgctca gcagggcgcg
     2221 ccgagagcag cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg
     2281 ccctgttcct gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc acgtcggcag
     2341 tcggctccct cgttgaccga atcaccgacc tctctcccca gggggatcca ccggagctta
     2401 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     2461 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     2521 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     2581 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     2641 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     2701 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     2761 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     2821 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     2881 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     2941 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     3001 actacctgag cacccagtcc aagctgagca aagaccccaa cgagaagcgc gatcacatgg
     3061 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
     3121 agacgcgtga attcactcct caggtgcagg ctgcctatca gaaggtggtg gctggtgtgg
     3181 ccaatgccct ggctcacaaa taccactgag atctttttcc ctctgccaaa aattatgggg
     3241 acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt attttcattg
     3301 caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc
     3361 atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc catatgctgg
     3421 ctgccatgaa caaaggttgg ctataaagag gtcatcagta tatgaaacag ccccctgctg
     3481 tccattcctt attccataga aaagccttga cttgaggtta gatttttttt atattttgtt
     3541 ttgtgttatt tttttcttta acatccctaa aattttcctt acatgtttta ctagccagat
     3601 ttttcctcct ctcctgacta ctcccagtca tagctgtccc tcttctctta tggagatccc
     3661 tcgacctgca gcccaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     3721 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     3781 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     3841 ggaaacctgt cgtgccagcg gatcgacagt actaagcttt actagggaca ggattggtga
     3901 cagaaaagcc ccatccttag gcctcctcct tcctagtctc ctgatattgg gtctaacccc
     3961 cacctcctgt taggcagatt ccttatctgg tgacacaccc ccatttcctg gagccatctc
     4021 tctccttgcc agaacctcta aggtttgctt acgatggagc cagagaggat cctgggaggg
     4081 agagcttggc agggggtggg agggaagggg gggatgcgtg acctgcccgg ttctcagtgg
     4141 ccaccctgcg ctaccctctc ccagaacctg agctgctctg acgcggctgt ctggtgcgtt
     4201 tcactgatcc tggtgctgca gcttccttac acttcccaag aggagaagca gtttggaaaa
     4261 acaaaatcag aataagttgg tcctgagttc taactttggc tcttcacctt tctagtcccc
     4321 aatttatatt gttcctccgt gcgtcagttt tacctgtgag ataaggccag tagccagccc
     4381 cgtcctggca gggctgtggt gaggaggggg gtgtccgtgt ggaaaactcc ctttgtgaga
     4441 atggtgcgtc ctaggtgttc accaggtcgt ggccgcctct actccctttc tctttctcca
     4501 tccttctttc cttaaagagt ccccagtgct atctgggaca tattcctccg cccagagcag
     4561 ggtcccgctt ccctaaggcc ctgctctggg cttctgggtt tgagtccttg gcaagcccag
     4621 gagaggcgct caggcttccc tgtccccctt cctcgtccac catctcatgc ccctggctct
     4681 cctgcccctt ccctacaggg gttcctggct ctgctct
//
