LOCUS       114411_AICSDP-34        4472 bp    DNA     linear   UNA 20-DEC-2018
DEFINITION  A new nucleotide sequence entered manually.
ACCESSION   urn.local.amandah.7r-9w3mn56
VERSION     urn.local.amandah.7r-9w3mn56
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     source          <1..>1000
                     /organism="Homo sapiens"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:9606"
                     /chromosome=1
                     /label="source Homo sapiens"
     gene            <1..>1000
                     /gene="TNNI1"
                     /gene_synonym="SSTNI; TNN1"
                     /note="troponin I1, slow skeletal type; Derived by
                     automated computational analysis using gene prediction
                     method: BestRefSeq."
                     /db_xref="GeneID:7135"
                     /db_xref="HGNC:HGNC:11945"
                     /db_xref="MIM:191042"
                     /label="TNNI1 gene"
     CDS             896..>1000
                     /gene="TNNI1"
                     /gene_synonym="SSTNI; TNN1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: BestRefSeq."
                     /codon_start=1
                     /product="troponin I, slow skeletal muscle"
                     /protein_id="NP_003272.3"
                     /db_xref="GI:56682969"
                     /db_xref="CCDS:CCDS1411.1"
                     /db_xref="GeneID:7135"
                     /db_xref="HGNC:HGNC:11945"
                     /db_xref="MIM:191042"
                     /translation="MPEVERKPKITASRKLLLKSLMLAKAKECWEQEHEEREAEKVRY
                     LAERIPTLQTRGLSLSALQDLCRELHAKVEVVDEERYDIEAKCLHNTREIKDLKLKVM
                     DLRGKFKRPPLRRVRVSADAMLRALLGSKHKVSMDLRANLKSVKKEDTEKERPVEVGD
                     WRKNVEAMSGMEGRKKMFDAAKSPTSQ"
                     /label="TNNI1 CDS"
     mRNA            896..>1000
                     /gene="TNNI1"
                     /gene_synonym="SSTNI; TNN1"
                     /product="troponin I1, slow skeletal type"
                     /note="Derived by automated computational analysis using
                     gene prediction method: BestRefSeq."
                     /transcript_id="NM_003281.3"
                     /db_xref="GI:56682968"
                     /db_xref="GeneID:7135"
                     /db_xref="HGNC:HGNC:11945"
                     /db_xref="MIM:191042"
                     /label="TNNI1 mRNA"
     misc_feature    984..>1000
                     /created_by="User"
                     /Color="#973bff"
                     /modified_by="User"
                     /label="crRNA1 Binding Site"
     misc_feature    complement(996..>1000)
                     /created_by="User"
                     /Color="#973bff"
                     /modified_by="User"
                     /label="crRNA2 Binding Site"
     misc_feature    1001..1006
                     /created_by="User"
                     /modified_by="User"
                     /label="Linker"
     misc_feature    1007..1015
                     /created_by="User"
                     /modified_by="User"
                     /label="Linker"
     misc_feature    complement(1019..1038)
                     /created_by="User"
                     /Color="#4096ee"
                     /modified_by="User"
                     /label="Tia1L-crRNA Binding Site"
     misc_feature    1040..1073
                     /created_by="User"
                     /Color="#0033aa"
                     /modified_by="User"
                     /label="loxP_forward_translation_linker_optimized"
     regulatory      1080..1218
                     /created_by="User"
                     /modified_by="User"
                     /label="SV40 Terminator"
     promoter        1220..1726
                     /created_by="User"
                     /modified_by="User"
                     /label="hPGK Promoter"
     ORF             1748..2458
                     /created_by="User"
                     /modified_by="User"
                     /label="mCherry"
     polyA_site      2475..2690
                     /created_by="User"
                     /modified_by="User"
                     /label="BGH_polyA_terminator"
     misc_feature    2693..2726
                     /created_by="User"
                     /Color="#0033aa"
                     /modified_by="User"
                     /label="loxP_forward_translation_linker_optimized"
     misc_feature    2730..2749
                     /created_by="User"
                     /Color="#33bbff"
                     /modified_by="User"
                     /label="Tia1L-crRNA Binding Site"
     misc_feature    2753..2758
                     /created_by="User"
                     /modified_by="User"
                     /label="Linker"
     ORF             2759..3472
                     /created_by="User"
                     /modified_by="User"
                     /label="mEGFP"
     CDS             <3473..3475
                     /gene="TNNI1"
                     /gene_synonym="SSTNI; TNN1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: BestRefSeq."
                     /codon_start=1
                     /product="troponin I, slow skeletal muscle"
                     /protein_id="NP_003272.3"
                     /db_xref="GI:56682969"
                     /db_xref="CCDS:CCDS1411.1"
                     /db_xref="GeneID:7135"
                     /db_xref="HGNC:HGNC:11945"
                     /db_xref="MIM:191042"
                     /translation="MPEVERKPKITASRKLLLKSLMLAKAKECWEQEHEEREAEKVRY
                     LAERIPTLQTRGLSLSALQDLCRELHAKVEVVDEERYDIEAKCLHNTREIKDLKLKVM
                     DLRGKFKRPPLRRVRVSADAMLRALLGSKHKVSMDLRANLKSVKKEDTEKERPVEVGD
                     WRKNVEAMSGMEGRKKMFDAAKSPTSQ"
                     /label="TNNI1 CDS"
     mRNA            <3473..3477
                     /gene="TNNI1"
                     /gene_synonym="SSTNI; TNN1"
                     /product="troponin I1, slow skeletal type"
                     /note="Derived by automated computational analysis using
                     gene prediction method: BestRefSeq."
                     /transcript_id="NM_003281.3"
                     /db_xref="GI:56682968"
                     /db_xref="GeneID:7135"
                     /db_xref="HGNC:HGNC:11945"
                     /db_xref="MIM:191042"
                     /label="TNNI1 mRNA"
     source          <3473..>4472
                     /organism="Homo sapiens"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:9606"
                     /chromosome=1
                     /label="source Homo sapiens"
     gene            <3473..>4472
                     /gene="TNNI1"
                     /gene_synonym="SSTNI; TNN1"
                     /note="troponin I1, slow skeletal type; Derived by
                     automated computational analysis using gene prediction
                     method: BestRefSeq."
                     /db_xref="GeneID:7135"
                     /db_xref="HGNC:HGNC:11945"
                     /db_xref="MIM:191042"
                     /label="TNNI1 gene"
     misc_feature    complement(<3473..3487)
                     /created_by="User"
                     /Color="#973bff"
                     /modified_by="User"
                     /label="crRNA2 Binding Site"
     misc_feature    <3473..3475
                     /created_by="User"
                     /Color="#973bff"
                     /modified_by="User"
                     /label="crRNA1 Binding Site"
ORIGIN      
        1 caccctctgc cctaccagct ttccccttgt caacccctca catggcctca ggctggccca
       61 ggagctcctg ttggagacca gtcagcttga cagacagaac attctagaat gctttgggag
      121 accagtgtgg gaagaaagaa agaagatttg ctcccttcct tcaggctgtt cccagtctga
      181 cgaagaaact aggacataaa gaaagctaga ccccagacgg agaaatgaag taaaaatctt
      241 gttcccagtg tgaagataat gtggagattt agggtaatgg tggagatgac tgcctggtca
      301 ccacagggaa aggagacagc agctcatctt ggttatagcc acacacgagc atagacatta
      361 gagaactgag ttcaaccacc cagcaacaaa ttattcaaag ttggttgaat tcacagtata
      421 tgtgccccaa attgataggc tctgtaagga acagggatga aggaacagtc tctggcccaa
      481 gaagcttcag aggagaagag ataagccaga caatatactg aggaaatgga aaagcagccg
      541 catttgcagg ggaagacttc ttggaagagg tggccttgag cctgctgagt gccagaccag
      601 gagggtggca tggagccttg gaggaggttt tgctgaggtg cactgaggtg ggaactagcc
      661 ccatagctgc cactttcacc cagtgctccc aagccacaca gcttcctttt tcctgtgggg
      721 cctcggcttg gcctgtgtcc acctgcctgg ctgagtccta ggaccttacc agctggggag
      781 gggcatcagg atttcttatc atccgaggag actggtttcc tatcacccag cttctcttga
      841 gctggggggc cgtccagcct ggtaagtccc tgatgtgtgt gccacttgtc tacaggagcg
      901 gcctgtggag gtgggtgact ggaggaagaa cgtggaggcc atgtctggca tggaaggccg
      961 gaagaagatg tttgatgccg ccaagtctcc gacctcacaa tccgggtccg ggtcccccgg
     1021 agaggttccc gacataccaa taacttcgta tagtgtagga tatacgaagt tatattagta
     1081 ttccgataac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     1141 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     1201 tgtatcttat catgtctgat tggggttgcg ccttttccaa ggcagccctg ggtttgcgca
     1261 gggacgcggc tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg
     1321 cacattcttc acgtccgttc gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc
     1381 cccggcgacg cttcctgctc cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg
     1441 ccggacgtga caaacggaag ccgcacgtct cactagtacc ctcgcagacg gacagcgcca
     1501 gggagcaatg gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct gctcagcagg
     1561 gcgcgccgag agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt
     1621 gtgggccctg ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc
     1681 ggcagtcggc tccctcgttg accgaatcac cgacctctct ccccaggggg atccaccgga
     1741 gcttaccatg gtctccaaag gagaagaaga taacatggcc atcatcaagg agttcatgcg
     1801 cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg
     1861 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc
     1921 cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt
     1981 gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg
     2041 ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct
     2101 gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg
     2161 ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga
     2221 ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta
     2281 cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta
     2341 caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca
     2401 gtacgaacgc gccgagggcc gccactccac cggcggaatg gatgaactct ataaatgagg
     2461 tactcctgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     2521 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     2581 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     2641 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg aaataacttc
     2701 gtatagtgta ggatatacga agttatatag gtatgtcggg aacctctccg ggtccggggt
     2761 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     2821 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     2881 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     2941 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
     3001 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     3061 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     3121 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     3181 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     3241 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     3301 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     3361 gagcacccag tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     3421 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtagaggta
     3481 cctagagacc agctcccttc tctggggtcc atcagagtct agaggataat gagggagtcc
     3541 acaatgggca ggcgggttgt acttagtgtg gtgcacggat gcacggacta aggcaccgat
     3601 gagactgatt tgagtcggct ctcctgggct cagtcctggc tccagcacca tgatacttgg
     3661 gcatgtcatt tctgtttggc ggcccttttt gttcccatta tctatgaaat tgggggctgt
     3721 atcaaatcag tggttctcca agtgtggtcc tgggaccagc agcgtctaca tcacctggga
     3781 tgcaaatgat cagcccccac cccagatcca ctaaattgga aactctgggg tgaggccagc
     3841 cttctaggtg attctgatgc acatgagagt ttgagaacca ctaggttaga gggtccctgg
     3901 gatcccttca tctctgactt tctgagaatc tatgcacttt taaatacctc ctggtgatgt
     3961 cctttatttg tgctccctgc gaagtaccca gcactcacag tacctgttcc caaggagcta
     4021 ccactcacag aagctaagac ttcccaccat ctgaggaaat agctggagaa actgggcaaa
     4081 gcaaggaaat caggccacgt aaaaccctct acggaagtgc tgagagaatg tgcaataggg
     4141 caggcatcca ccagacagca ctggtcagag caggctttct ggagaggagg agctctctga
     4201 ggatgcgacg agcgagtgtt agctgataaa gggaagggca tgttgaaggg ctgatggtgc
     4261 aacagaagca tggaggtggg aattaagcag ccacagagta gcatggcaag gctaggtaag
     4321 agctgagggc cgcagtgtgg aggagcggtg tgaggggtcc caaaagggga gcaactgtgc
     4381 ccgagggccg agaagctgag gttttgtagg agcccttatg aatgaagatc cagtccgggg
     4441 tgcgctaggg ctgagaagcc ctggggactg ca
//
