LOCUS       #873_(modified)        18656 bp    DNA     circular UNA 19-MAR-1980
DEFINITION  .
ACCESSION   urn.local...mh-4xsn6kd (modified)
VERSION     urn.local...mh-4xsn6kd
KEYWORDS    .
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
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COMMENT     LSOWNER|
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FEATURES             Location/Qualifiers
     misc_recomb     414..447
                     /vntifkey="86"
                     /label=FRT\3
     misc_feature    495..1698
                     /vntifkey="21"
                     /label=Insulator\1
     misc_feature    1711..2914
                     /vntifkey="21"
                     /label=Insulator\2
     misc_feature    2930..2935
                     /vntifkey="21"
                     /label=Age
     misc_feature    2936..2941
                     /vntifkey="21"
                     /label=Cla
     misc_feature    2943..2948
                     /vntifkey="21"
                     /label=Eag
     misc_feature    2944..2951
                     /vntifkey="21"
                     /label=Fse
     misc_feature    3046..3478
                     /vntifkey="21"
                     /label=TRE2\from\pTRE2-Hyg
     misc_feature    3359..3418
                     /vntifkey="21"
                     /label=pCMV\min
     misc_recomb     complement(3529..3562)
                     /vntifkey="86"
                     /label=LoxP
     misc_feature    3563..3568
                     /vntifkey="21"
                     /label=Nru
     misc_feature    3570..3580
                     /vntifkey="21"
                     /label=Stops
     misc_feature    3595..3601
                     /vntifkey="21"
                     /label=BbvCI
     misc_feature    3605..3628
                     /vntifkey="21"
                     /label=ORF
     misc_feature    3629..3639
                     /vntifkey="21"
                     /label=Stops
     misc_feature    3640..3689
                     /vntifkey="21"
                     /label=Synthetic\pA
     misc_feature    3640..3645
                     /vntifkey="21"
                     /label=Element\1
     misc_feature    3646..3668
                     /vntifkey="21"
                     /label=23\bp
     misc_difference 3663
                     /vntifkey="85"
                     /label=A\to\C
     misc_feature    3669..3688
                     /vntifkey="21"
                     /label=Element\2
     polyA_signal    3690..4176
                     /vntifkey="25"
                     /label=hGHpA
     misc_feature    3979..3985
                     /Recognition_pattern="CC^TNAGG"
                     /annotation_group="CCTNAGG"
                     /vntifkey="21"
                     /label=Bsu36I
                     /note="Geneious type: restriction site"
     misc_difference 3985
                     /vntifkey="85"
                     /label=C\to\G\change
     misc_feature    4177..4183
                     /vntifkey="21"
                     /label=BbvCI
     polyA_signal    4184..4651
                     /vntifkey="25"
                     /label=PGK\pA
     misc_feature    4652..4659
                     /vntifkey="21"
                     /label=Pac
     misc_feature    4661..4671
                     /vntifkey="21"
                     /label=Stops
     misc_recomb     complement(4673..4706)
                     /vntifkey="86"
                     /label=LoxP
     modified_base   complement(4708..4713)
                     /vntifkey="23"
                     /label=MluI
     misc_feature    4714..4722
                     /vntifkey="21"
                     /label=Kozak
     CDS             4723..6075
                     /vntifkey="4"
                     /label=GCaMP6\slow
     modified_base   complement(6076..6081)
                     /vntifkey="23"
                     /label=MluI
     misc_feature    6082..6087
                     /vntifkey="21"
                     /label=SnaBI
     misc_feature    6088..6093
                     /vntifkey="21"
                     /label=HindIII
     misc_feature    6090..6698
                     /vntifkey="21"
                     /label=WPRE
     misc_feature    6094
                     /vntifkey="21"
                     /label=A\to\G\change
     misc_feature    6694
                     /vntifkey="21"
                     /label=T\to\C\change
     polyA_signal    6712..6926
                     /vntifkey="25"
                     /label=bGHpA
     misc_feature    6927..6932
                     /vntifkey="21"
                     /label=Pml
     misc_feature    6950..8153
                     /vntifkey="21"
                     /label=Insulator\3
     misc_feature    8160..9363
                     /vntifkey="21"
                     /label=Insulator\4
     misc_feature    9369
                     /created_by="User"
                     /vntifkey="21"
                     /label=deleted\A\from\sequencing
     misc_feature    9369^9370
                     /Original_Bases="A"
                     /vntifkey="21"
                     /label=A
                     /note="Geneious type: Editing History Deletion"
     misc_feature    9390..11137
                     /vntifkey="21"
                     /label=CAG
     misc_feature    complement(9449..9470)
                     /Sequence="CGTAAGTTATGTAACGCGGAAC"
                     /Hairpin_Tm="41.0"
                     /Tm="57.8"
                     /Self_Dimer_Tm="8.5"
                     /%GC="45.5"
                     /created_by="primer3"
                     /vntifkey="21"
                     /label=MG-107-R
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(9664..9683)
                     /Sequence="ATGCCAGGCGGGCCATTTAC"
                     /Hairpin_Tm="63.9"
                     /Tm="63.5"
                     /Self_Dimer_Tm="15.7"
                     /%GC="60.0"
                     /created_by="primer3"
                     /vntifkey="21"
                     /label=MG-311-R
                     /note="Geneious type: primer_bind_reverse"
     misc_recomb     11144..11177
                     /vntifkey="86"
                     /label=Lox2272
     misc_feature    11180..11203
                     /vntifkey="21"
                     /label=ORF
     misc_feature    11204..11214
                     /vntifkey="21"
                     /label=Stops
     misc_feature    11215..11264
                     /vntifkey="21"
                     /label=Synthetic\pA
     misc_feature    11215..11220
                     /vntifkey="21"
                     /label=Element\1
     misc_feature    11221..11243
                     /vntifkey="21"
                     /label=23\bp
     misc_difference 11238
                     /vntifkey="85"
                     /label=A\to\C
     misc_feature    11244..11263
                     /vntifkey="21"
                     /label=Element\2
     polyA_signal    11265..11751
                     /vntifkey="25"
                     /label=hGHpA
     primer          11331..11353
                     /vntifkey="27"
                     /label=Mg-1077F
     misc_feature    11554..11560
                     /Recognition_pattern="CC^TNAGG"
                     /annotation_group="CCTNAGG"
                     /vntifkey="21"
                     /label=Bsu36I
                     /note="Geneious type: restriction site"
     misc_difference 11560
                     /vntifkey="85"
                     /label=C\to\G\change
     misc_feature    11752..11758
                     /vntifkey="21"
                     /label=BbvCI
     misc_feature    11759..11769
                     /vntifkey="21"
                     /label=Stops
     misc_feature    11770..12031
                     /vntifkey="21"
                     /label=TK\pA
     primer          11845..11867
                     /vntifkey="27"
                     /label=MG-1078R
     misc_feature    12032..12037
                     /vntifkey="21"
                     /label=Nru
     misc_recomb     12049..12082
                     /vntifkey="86"
                     /label=Lox2272
     misc_feature    12104..13532
                     /vntifkey="21"
                     /label=tdTomato
     misc_feature    13525..13530
                     /vntifkey="21"
                     /label=BsrGI
     misc_feature    13533..13598
                     /vntifkey="21"
                     /label=P2A
     CDS             13599..14345
                     /vntifkey="4"
                     /label=tTA2
     misc_feature    14116..14121
                     /Recognition_pattern="C^AATTG"
                     /annotation_group="CAATTG"
                     /vntifkey="21"
                     /label=MfeI
                     /note="Geneious type: restriction site"
     misc_feature    14370..14375
                     /vntifkey="21"
                     /label=SnaBI
     misc_feature    14376..14381
                     /vntifkey="21"
                     /label=HindIII
     misc_feature    14378..14986
                     /vntifkey="21"
                     /label=WPRE
     misc_feature    14382
                     /vntifkey="21"
                     /label=A\to\G\change
     misc_feature    14982
                     /vntifkey="21"
                     /label=T\to\C\change
     polyA_signal    15000..15214
                     /vntifkey="25"
                     /label=bGHpA
     misc_feature    15215..15220
                     /vntifkey="21"
                     /label=Pml
     misc_feature    15227..15261
                     /vntifkey="21"
                     /label=AttB
     misc_feature    15275..15800
                     /vntifkey="21"
                     /label=PGK
     misc_feature    15808..15813
                     /vntifkey="21"
                     /label=Kozak
     CDS             15814..16326
                     /vntifkey="4"
                     /label=Hygro\1
     misc_feature    16327..16332
                     /vntifkey="21"
                     /label=SD
     misc_recomb     16354..16387
                     /vntifkey="86"
                     /label=FRT\5
     rep_origin      16781..17448
                     /vntifkey="33"
                     /label=ori
     CDS             17599..18456
                     /vntifkey="4"
                     /label=Amp
     misc_binding    3054..3072
                     /vntifkey="20"
                     /label=TRE
     misc_binding    3096..3114
                     /vntifkey="20"
                     /label=TRE
     misc_binding    3138..3156
                     /vntifkey="20"
                     /label=TRE
     misc_binding    3180..3198
                     /vntifkey="20"
                     /label=TRE
     misc_binding    3222..3240
                     /vntifkey="20"
                     /label=TRE
     misc_binding    3264..3282
                     /vntifkey="20"
                     /label=TRE
     misc_feature    3306..3324
                     /vntifkey="21"
                     /label=TRE
BASE COUNT     3922 a      5076 c      5324 g      4334 t 
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt accgaagttc 
      421 ctattcttca aatagtatag gaacttcttt cggccggcca atcgattgga tccgtttatt 
      481 aagaattcga gctcgcccca tcctcactga ctccgtcctg gagttggatg agagataatg 
      541 gccttacgtt gtgccagggg agggtcgggc tggatttagc aagatttacc ttctccaaag 
      601 agcggtgctg cagtggcaca gctgcccacg gaggtggggg ggtcaccgtc cctggaggtg 
      661 atgaagaact gtggggatgt ggcactgagg gacatggcca gtgggcacgg tgggtgggtt 
      721 ggggttggtc ttggggatct tggagggctt ttccagcctt catgatttga cgattgtatg 
      781 aacatctaca tggcaattct ccagctgcct gtcccagtcc tactgaccca gctgtatctc 
      841 tccaggcaag ctcttccacc ccttctgctt gcatccagac accatcaaac atgcaggctc 
      901 agacacaggg accagcagtg tctgtggcct ttttgtgctc ctctccatgc tgggttttaa 
      961 cttgctcttt gtccttctat cctatcttct tatccttaag gctgttctga acgctgtgac 
     1021 ttggagagtg tcccagagcc ctcaacacct gcatgtccca cgtccatgct gtcctgcact 
     1081 tccttatccc caagatctgc ctctccgtga tgcactgaat tggcaaacat gtgtcacccc 
     1141 agaccaacaa tgtcacagca aactccccct tgataggaca agggggaatg gctttacact 
     1201 gagacagggg aggtttgggt tggatatgag gaggcagttt ttcccccaga gggtggtgac 
     1261 gcactgaaca ggttgcccaa ggaggctgtg gatgccccat ccctgcaggc attcaaggcc 
     1321 aggctggatg tggctctggg cagcctgggc tgctggttga tgaccctgca catagcaggg 
     1381 ggttggatct ggatgagcac tgtgctcctt tgcaacccag gccgttctat gattctgtca 
     1441 ttctaaatct ctctttcagc ctaaagcttt ttccccgtat ccccccaggt gtctgcaggc 
     1501 tcaaagagca gcgagaagcg ttcagaggaa agcgatcccg tgccaccttc cccgtgcccg 
     1561 ggctgtcccc gcacgctgcc ggctcgggga tgcgggggga gcgccggacc ggagcggagc 
     1621 cccgggcggc tcgctgctgc cccctagcgg gggagggacg taattacatc cctgggggct 
     1681 ttgggggggg gctgtcccga gctcggtacc gccccatcct cactgactcc gtcctggagt 
     1741 tggatgagag ataatggcct tacgttgtgc caggggaggg tcgggctgga tttagcaaga 
     1801 tttaccttct ccaaagagcg gtgctgcagt ggcacagctg cccacggagg tgggggggtc 
     1861 accgtccctg gaggtgatga agaactgtgg ggatgtggca ctgagggaca tggccagtgg 
     1921 gcacggtggg tgggttgggg ttggtcttgg ggatcttgga gggcttttcc agccttcatg 
     1981 atttgacgat tgtatgaaca tctacatggc aattctccag ctgcctgtcc cagtcctact 
     2041 gacccagctg tatctctcca ggcaagctct tccacccctt ctgcttgcat ccagacacca 
     2101 tcaaacatgc aggctcagac acagggacca gcagtgtctg tggccttttt gtgctcctct 
     2161 ccatgctggg ttttaacttg ctctttgtcc ttctatccta tcttcttatc cttaaggctg 
     2221 ttctgaacgc tgtgacttgg agagtgtccc agagccctca acacctgcat gtcccacgtc 
     2281 catgctgtcc tgcacttcct tatccccaag atctgcctct ccgtgatgca ctgaattggc 
     2341 aaacatgtgt caccccagac caacaatgtc acagcaaact cccccttgat aggacaaggg 
     2401 ggaatggctt tacactgaga caggggaggt ttgggttgga tatgaggagg cagtttttcc 
     2461 cccagagggt ggtgacgcac tgaacaggtt gcccaaggag gctgtggatg ccccatccct 
     2521 gcaggcattc aaggccaggc tggatgtggc tctgggcagc ctgggctgct ggttgatgac 
     2581 cctgcacata gcagggggtt ggatctggat gagcactgtg ctcctttgca acccaggccg 
     2641 ttctatgatt ctgtcattct aaatctctct ttcagcctaa agctttttcc ccgtatcccc 
     2701 ccaggtgtct gcaggctcaa agagcagcga gaagcgttca gaggaaagcg atcccgtgcc 
     2761 accttccccg tgcccgggct gtccccgcac gctgccggct cggggatgcg gggggagcgc 
     2821 cggaccggag cggagccccg ggcggctcgc tgctgccccc tagcggggga gggacgtaat 
     2881 tacatccctg ggggctttgg gggggggctg tcccggtacc cggggatcca ccggtatcga 
     2941 ttcggccggc cgacgtcggc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat 
     3001 tgctttattt gtaaccatta taagctgcaa taaacaagtt tcgagtttac cactccctat 
     3061 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg 
     3121 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact 
     3181 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga 
     3241 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt 
     3301 accactccct atcagtgata gagaaaagtg aaagtcgagc tcggtacccg ggtcgaggta 
     3361 ggcgtgtacg gtgggaggcc tatataagca gagctcgttt agtgaaccgt cagatcgcct 
     3421 ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga tccagcctcc 
     3481 gcggccccga attcgagctc ggtacccggg gatcaaattc aattaaatat aacttcgtat 
     3541 agcatacatt atacgaagtt attcgcgagt gaataaatga aagcttgcag atcccctcag 
     3601 caccatggct agcggcagcc tcggagtttg aatagataga ataaaatatc tttattttca 
     3661 ttccatctgt gtgttggttt tttgtgtgag atctacgggt ggcatccctg tgacccctcc 
     3721 ccagtgcctc tcctggccct ggaagttgcc actccagtgc ccaccagcct tgtcctaata 
     3781 aaattaagtt gcatcatttt gtctgactag gtgtccttct ataatattat ggggtggagg 
     3841 ggggtggtat ggagcaaggg gcaagttggg aagacaacct gtagggcctg cggggtctat 
     3901 tgggaaccaa gctggagtgc agtggcacaa tcttggctca ctgcaatctc cgcctcctgg 
     3961 gttcaagcga ttctcctgcc tcaggctccc gagttgttgg gattccaggc atgcatgacc 
     4021 aggctcagct aatttttgtt tttttggtag agacggggtt tcaccatatt ggccaggctg 
     4081 gtctccaact cctaatctca ggtgatctac ccaccttggc ctcccaaatt gctgggatta 
     4141 caggcgtgaa ccactgctcc cttccctgtc cttctgcctc agcgctgtaa gtctgcagaa 
     4201 attgatgatc tattaaacaa taaagatgtc cactaaaatg gaagtttttc ctgtcatact 
     4261 ttgttaagaa gggtgagaac agagtaccta cattttgaat ggaaggattg gagctacggg 
     4321 ggtgggggtg gggtgggatt agataaatgc ctgctcttta ctgaaggctc tttactattg 
     4381 ctttatgata atgtttcata gttggatatc ataatttaaa caagcaaaac caaattaagg 
     4441 gccagctcat tcctcccact catgatctat agatctatag atctctcgtg ggatcattgt 
     4501 ttttctcttg attcccactt tgtggttcta agtactgtgg tttccaaatg tgtcagtttc 
     4561 atagcctgaa gaacgagatc agcagcctct gttccacata cacttcattc tcagtattgt 
     4621 tttgccaagt tctaattcca tcagaaagct tttaattaac tgaatagcta acataacttc 
     4681 gtatagcata cattatacga agttatcacg cgtgccgcca ccatgggttc tcatcatcat 
     4741 catcatcatg gtatggctag catgactggt ggacagcaaa tgggtcggga tctgtacgac 
     4801 gatgacgata aggatctcgc caccatggtc gactcatcac gtcgtaagtg gaataagaca 
     4861 ggtcacgcag tcagagctat aggtcggctg agctcactcg agaacgtcta tatcaaggcc 
     4921 gacaagcaga agaacggcat caaggcgaac ttccacatcc gccacaacat cgaggacggc 
     4981 ggcgtgcagc tcgcctacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg 
     5041 ctgcccgaca accactacct gagcgtgcag tccaaacttt cgaaagaccc caacgagaag 
     5101 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac 
     5161 gagctgtaca agggcggtac cggagggagc atggtgagca agggcgagga gctgttcacc 
     5221 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg 
     5281 tccggcgagg gtgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc 
     5341 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag 
     5401 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc 
     5461 gaaggctaca tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc 
     5521 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac 
     5581 ttcaaggagg acggcaacat cctggggcac aagctggagt acaacctgcc ggaccaactg 
     5641 actgaagagc agatcgcaga atttaaagag gctttctccc tatttgacaa ggacggggat 
     5701 gggacaataa caaccaagga gctggggacg gtgatgcggt ctctggggca gaaccccaca 
     5761 gaagcagagc tgcaggacat gatcaatgaa gtagatgccg acggtgacgg cacaatcgac 
     5821 ttccctgagt tcctgacaat gatggcaaga aaaatgaaat acagggacac ggaagaagaa 
     5881 attagagaag cgttcggtgt gtttgataag gatggcaatg gctacatcag tgcagcagag 
     5941 cttcgccacg tgatgacaaa ccttggagag aagttaacag atgaagaggt tgatgaaatg 
     6001 atcagggaag cagacatcga tggggatggt caggtaaact acgaagagtt tgtacaaatg 
     6061 atgacagcga agtaaacgcg ttacgtaaag cttgtcgata atcaacctct ggattacaaa 
     6121 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac 
     6181 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc 
     6241 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt 
     6301 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc 
     6361 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc 
     6421 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg 
     6481 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctatgttgc cacctggatt 
     6541 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc 
     6601 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt 
     6661 cggatctccc tttgggccgc ctccccgcat cgacaccgag cgctgctcga ggcctcgact 
     6721 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg 
     6781 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg 
     6841 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg 
     6901 gaagacaata gcaggcatgc tggggacacg tgggatcctc tagtctagag ccccatcctc 
     6961 actgactccg tcctggagtt ggatgagaga taatggcctt acgttgtgcc aggggagggt 
     7021 cgggctggat ttagcaagat ttaccttctc caaagagcgg tgctgcagtg gcacagctgc 
     7081 ccacggaggt gggggggtca ccgtccctgg aggtgatgaa gaactgtggg gatgtggcac 
     7141 tgagggacat ggccagtggg cacggtgggt gggttggggt tggtcttggg gatcttggag 
     7201 ggcttttcca gccttcatga tttgacgatt gtatgaacat ctacatggca attctccagc 
     7261 tgcctgtccc agtcctactg acccagctgt atctctccag gcaagctctt ccaccccttc 
     7321 tgcttgcatc cagacaccat caaacatgca ggctcagaca cagggaccag cagtgtctgt 
     7381 ggcctttttg tgctcctctc catgctgggt tttaacttgc tctttgtcct tctatcctat 
     7441 cttcttatcc ttaaggctgt tctgaacgct gtgacttgga gagtgtccca gagccctcaa 
     7501 cacctgcatg tcccacgtcc atgctgtcct gcacttcctt atccccaaga tctgcctctc 
     7561 cgtgatgcac tgaattggca aacatgtgtc accccagacc aacaatgtca cagcaaactc 
     7621 ccccttgata ggacaagggg gaatggcttt acactgagac aggggaggtt tgggttggat 
     7681 atgaggaggc agtttttccc ccagagggtg gtgacgcact gaacaggttg cccaaggagg 
     7741 ctgtggatgc cccatccctg caggcattca aggccaggct ggatgtggct ctgggcagcc 
     7801 tgggctgctg gttgatgacc ctgcacatag cagggggttg gatctggatg agcactgtgc 
     7861 tcctttgcaa cccaggccgt tctatgattc tgtcattcta aatctctctt tcagcctaaa 
     7921 gctttttccc cgtatccccc caggtgtctg caggctcaaa gagcagcgag aagcgttcag 
     7981 aggaaagcga tcccgtgcca ccttccccgt gcccgggctg tccccgcacg ctgccggctc 
     8041 ggggatgcgg ggggagcgcc ggaccggagc ggagccccgg gcggctcgct gctgccccct 
     8101 agcgggggag ggacgtaatt acatccctgg gggctttggg ggggggctgt ccctctagag 
     8161 ccccatcctc actgactccg tcctggagtt ggatgagaga taatggcctt acgttgtgcc 
     8221 aggggagggt cgggctggat ttagcaagat ttaccttctc caaagagcgg tgctgcagtg 
     8281 gcacagctgc ccacggaggt gggggggtca ccgtccctgg aggtgatgaa gaactgtggg 
     8341 gatgtggcac tgagggacat ggccagtggg cacggtgggt gggttggggt tggtcttggg 
     8401 gatcttggag ggcttttcca gccttcatga tttgacgatt gtatgaacat ctacatggca 
     8461 attctccagc tgcctgtccc agtcctactg acccagctgt atctctccag gcaagctctt 
     8521 ccaccccttc tgcttgcatc cagacaccat caaacatgca ggctcagaca cagggaccag 
     8581 cagtgtctgt ggcctttttg tgctcctctc catgctgggt tttaacttgc tctttgtcct 
     8641 tctatcctat cttcttatcc ttaaggctgt tctgaacgct gtgacttgga gagtgtccca 
     8701 gagccctcaa cacctgcatg tcccacgtcc atgctgtcct gcacttcctt atccccaaga 
     8761 tctgcctctc cgtgatgcac tgaattggca aacatgtgtc accccagacc aacaatgtca 
     8821 cagcaaactc ccccttgata ggacaagggg gaatggcttt acactgagac aggggaggtt 
     8881 tgggttggat atgaggaggc agtttttccc ccagagggtg gtgacgcact gaacaggttg 
     8941 cccaaggagg ctgtggatgc cccatccctg caggcattca aggccaggct ggatgtggct 
     9001 ctgggcagcc tgggctgctg gttgatgacc ctgcacatag cagggggttg gatctggatg 
     9061 agcactgtgc tcctttgcaa cccaggccgt tctatgattc tgtcattcta aatctctctt 
     9121 tcagcctaaa gctttttccc cgtatccccc caggtgtctg caggctcaaa gagcagcgag 
     9181 aagcgttcag aggaaagcga tcccgtgcca ccttccccgt gcccgggctg tccccgcacg 
     9241 ctgccggctc ggggatgcgg ggggagcgcc ggaccggagc ggagccccgg gcggctcgct 
     9301 gctgccccct agcgggggag ggacgtaatt acatccctgg gggctttggg ggggggctgt 
     9361 ccctctagag tcgacggcgc gccctgcagg aatctagtta ttaatagtaa tcaattacgg 
     9421 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc 
     9481 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca 
     9541 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg 
     9601 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg 
     9661 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt 
     9721 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc cccacgttct 
     9781 gcttcactct ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt 
     9841 aattattttg tgcagcgatg ggggcggggg gggggggggg gcgcgcgcca ggcggggcgg 
     9901 ggcggggcga ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg 
     9961 gcgcgctccg aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc 
    10021 gaagcgcgcg gcgggcgggg agtcgctgcg acgctgcctt cgccccgtgc cccgctccgc 
    10081 cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg 
    10141 gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc 
    10201 ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag 
    10261 cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct 
    10321 gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg 
    10381 cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa 
    10441 aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg 
    10501 gctgcaaccc cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc 
    10561 ggggctccgt acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt 
    10621 gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aggggcgcgg 
    10681 cggcccccgg agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt gccttttatg 
    10741 gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa atctgtgcgg agccgaaatc 
    10801 tgggaggcgc cgccgcaccc cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag 
    10861 gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct 
    10921 ccagcctcgg ggctgtccgc ggggggacgg ctgccttcgg gggggacggg gcagggcggg 
    10981 gttcggcttc tggcgtgtga ccggcggctc tagagcctct gctaaccatg ttcatgcctt 
    11041 cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg 
    11101 caaagaattg atttgatacc gcgggccccg cgtggacggt accataactt cgtataaagt 
    11161 atcctatacg aagttatcca tggctagcgg cagcctcgga gtttgaatag atagaataaa 
    11221 atatctttat tttcattcca tctgtgtgtt ggttttttgt gtgagatcta cgggtggcat 
    11281 ccctgtgacc cctccccagt gcctctcctg gccctggaag ttgccactcc agtgcccacc 
    11341 agccttgtcc taataaaatt aagttgcatc attttgtctg actaggtgtc cttctataat 
    11401 attatggggt ggaggggggt ggtatggagc aaggggcaag ttgggaagac aacctgtagg 
    11461 gcctgcgggg tctattggga accaagctgg agtgcagtgg cacaatcttg gctcactgca 
    11521 atctccgcct cctgggttca agcgattctc ctgcctcagg ctcccgagtt gttgggattc 
    11581 caggcatgca tgaccaggct cagctaattt ttgttttttt ggtagagacg gggtttcacc 
    11641 atattggcca ggctggtctc caactcctaa tctcaggtga tctacccacc ttggcctccc 
    11701 aaattgctgg gattacaggc gtgaaccact gctcccttcc ctgtccttct gcctcagcta 
    11761 attgagtagg ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg 
    11821 ctatgacggc aataaaaaga cagaataaaa cgcacgggtg ttgggtcgtt tgttcataaa 
    11881 cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc 
    11941 caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca 
    12001 gggctcgcag ccaacgtcgg ggcggcaggc ctcgcgaggg tttctagaat aacttcgtat 
    12061 aaagtatcct atacgaagtt atacgcgcgc gtactagcgc caccatggtg agcaagggcg 
    12121 aggaggtcat caaagagttc atgcgcttca aggtgcgcat ggagggctcc atgaacggcc 
    12181 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca 
    12241 agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tccccccagt 
    12301 tcatgtacgg ctccaaggcg tacgtgaagc accccgccga catccccgat tacaagaagc 
    12361 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggtctgg 
    12421 tgaccgtgac ccaggactcc tccctgcagg acggcacgct gatctacaag gtgaagatgc 
    12481 gcggcaccaa cttccccccc gacggccccg taatgcagaa gaagaccatg ggctgggagg 
    12541 cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc caccaggccc 
    12601 tgaagctgaa ggacggcggc cactacctgg tggagttcaa gaccatctac atggccaaga 
    12661 agcccgtgca actgcccggc tactactacg tggacaccaa gctggacatc acctcccaca 
    12721 acgaggacta caccatcgtg gaacagtacg agcgctccga gggccgccac cacctgttcc 
    12781 tggggcatgg taccggcagc accggcagcg gcagctccgg caccgcctcc tccgaggaca 
    12841 acaacatggc cgtgattaag gagtttatga ggtttaaagt caggatggag ggcagcatga 
    12901 acgggcacga gtttgagatt gaaggagagg gggaagggag gccctatgag ggcacccaga 
    12961 cagcaaagct gaaagtgaca aagggcggcc ctctgccatt cgcatgggac atcctgtctc 
    13021 ctcagtttat gtatggaagt aaagcctacg tgaaacaccc tgctgacatt ccagattaca 
    13081 agaaactgag cttcccagag gggtttaagt gggaaagagt gatgaacttc gaagacgggg 
    13141 gcctggtgac tgtcacccag gacagctctc tgcaggacgg cactctgatc tataaggtga 
    13201 aaatgcgcgg caccaatttt ccccctgatg gcccagtcat gcagaagaag acaatgggct 
    13261 gggaggcttc aacagaaagg ctgtacccca gagacggcgt gctgaaggga gagatccacc 
    13321 aggcactgaa gctgaaagat ggagggcatt atctggtcga attcaaaacc atctacatgg 
    13381 ccaagaaacc cgtgcagctg cctggctact attacgtcga cacaaagctg gatattacta 
    13441 gccataacga agactacacc attgtggagc agtatgagcg gagcgagggg aggcaccatc 
    13501 tgtttctgta cggcatggac gagctgtaca agggaagcgg agctactaac ttcagcctgc 
    13561 tgaagcaggc tggagacgtg gaggagaacc ctggacctat gtctagactg gacaagagca 
    13621 aagtcataaa ctctgctctg gaattactca atgaagtcgg tatcgaaggc ctgacgacaa 
    13681 ggaaactcgc tcaaaagctg ggagttgagc agcctaccct gtactggcac gtgaagaaca 
    13741 agcgggccct gctcgatgcc ctggcaatcg agatgctgga caggcatcat acccacttct 
    13801 gccccctgga aggcgagtca tggcaagact ttctgcggaa caacgccaag tcattccgct 
    13861 gtgctctcct ctcacatcgc gacggggcta aagtgcatct cggcacccgc ccaacagaga 
    13921 aacagtacga aaccctggaa aatcagctcg cgttcctgtg tcagcaaggc ttctccctgg 
    13981 agaacgcact gtacgctctg tccgccgtgg gccactttac actgggctgc gtattggagg 
    14041 atcaggagca tcaagtagca aaagaggaaa gagagacacc taccaccgat tctatgcccc 
    14101 cacttctgag acaagcaatt gagctgttcg accatcaggg agccgaacct gccttccttt 
    14161 tcggcctgga actaatcata tgtggcctgg agaaacagct aaagtgcgaa agcggcgggc 
    14221 cggccgacgc ccttgacgat tttgacttag acatgctccc agccgatgcc cttgacgact 
    14281 ttgaccttga tatgctgcct gctgacgctc ttgacgattt tgaccttgac atgctccccg 
    14341 ggtaactaag taaggatcaa atggcgcgtt acgtaaagct tgtcgataat caacctctgg 
    14401 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat 
    14461 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt 
    14521 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca 
    14581 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg 
    14641 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg 
    14701 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca 
    14761 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tatgttgcca 
    14821 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc 
    14881 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc 
    14941 agacgagtcg gatctccctt tgggccgcct ccccgcatcg acaccgagcg ctgctcgagg 
    15001 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct 
    15061 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc 
    15121 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg 
    15181 aggattggga agacaatagc aggcatgctg gggacacgtg ggatccggtg ccagggcgtg 
    15241 cccttgggct ccccgggcgc gtttgggcgg ccgcatcgaa ttctaccggg taggggaggc 
    15301 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta 
    15361 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc 
    15421 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc 
    15481 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct 
    15541 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca 
    15601 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg 
    15661 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc 
    15721 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg 
    15781 cctttcgacc tgcaatcgcc gctagccgcc accatgaaaa agcctgaact caccgcgacg 
    15841 tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg 
    15901 gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg 
    15961 gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg 
    16021 gccgcgctcc cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat 
    16081 tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc 
    16141 gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag 
    16201 acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat 
    16261 ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc 
    16321 gtcagtgtaa gtgctagccc ctaggcctga atcgaagttc ctattcttca aaaggtatag 
    16381 gaacttcgag ctcgtcgact gcagaggcct gcatgcaagc ttggcgtaat catggtcata 
    16441 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag 
    16501 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg 
    16561 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca 
    16621 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc 
    16681 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 
    16741 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 
    16801 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 
    16861 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 
    16921 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 
    16981 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 
    17041 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 
    17101 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 
    17161 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 
    17221 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac 
    17281 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 
    17341 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 
    17401 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 
    17461 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 
    17521 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 
    17581 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 
    17641 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 
    17701 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 
    17761 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 
    17821 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 
    17881 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 
    17941 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 
    18001 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 
    18061 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 
    18121 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 
    18181 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 
    18241 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 
    18301 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 
    18361 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 
    18421 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 
    18481 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 
    18541 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac 
    18601 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc 
//
