LOCUS       Exported               10789 bp ds-DNA     circular SYN 12-JUL-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10789)
  AUTHORS   Sibley Lab WUSTL
  TITLE     Direct Submission
  JOURNAL   Exported Jul 20, 2018 from SnapGene 4.2.0
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10789
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(21..37)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        511..615
                     /gene="bla"
                     /label=AmpR promoter
     CDS             616..1476
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1647..2235
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2381..2397
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2417..2437
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    2438..3191
                     /label=upUPRT 5' HR flank
     protein_bind    3211..3244
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    3245..4235
                     /label=pDHFR
     CDS             4236..6068
                     /codon_start=1
                     /label=DHFR-TS* (PyrR)
                     /translation="MQKPVCLVVAMTPKRGIGINNGLPWPHLTTDFKHFRRVTKTTPEE
                     ASRLNGWLPRKFAKTGDSGLPSPSVGKRFNAVVMGRKNWESMPRKFRPLVDRLNIVVSS
                     SLKEEDIAAEKPQAEGQQRVRVCASLPAALSLLEEEYKDSVDQIFVVGGAGLYEAALSL
                     GVASHLYITRVAREFPCDVFFPAFPGDDILSNKSTAAQAAAPAESVFVPFCPELGREKD
                     NEATYRPIFISKTFSDNGVPYDFVVLEKRRKTDDAATAEPSNAMSSLTSTRETTPVHGL
                     QAPSSAAAIAPVLAWMDEEDRKKREQKELIRAVPHVHFRGHEEFQYLDLIADIINNGRT
                     MDDRTGVGVISKFGCTMRYSLDQAFPLLTTKRVFWKGVLEELLWFIRGDTNANHLSEKG
                     VKIWDKNVTREFLDSRNLPHREVGDIGPGYGFQWRHFGAAYKDMHTDYTGQGVDQLKNV
                     IQMLRTNPTDRRMLMTAWNPAALDEMALPPCHLLCQFYVNDQKELSCIMYQRSCDVGLG
                     VPFNIASYSLLTLMVAHVCNLKPKEFIHFMGNTHVYTNHVEALKEQLRREPRPFPIVNI
                     LNKERIKEIDDFTAEDFEVVGYVPHGRIQMEMAV"
     protein_bind    6076..6109
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    6110..7132
                     /label=DHFR 3' UTR
     primer_bind     complement(6569..6592)
                     /label=JBR121
                     /note="GA for gblock FLUC.  Use with JBR120.  shortened 
                     primer to avoid self annealing.  Can also use with JBR118 
                     to amplify sequence."
     primer_bind     complement(6569..6592)
                     /label=JBR119
                     /note="GA with gblock. use with JBR118 to amplify Fluc 
                     expression cassette."
     primer_bind     7098..7157
                     /label=JBR118
                     /note="GA with gBlock. use with JBR119 to amplify 
                     expression cassette from gBlock"
     primer_bind     complement(7111..7170)
                     /label=JBR116
                     /note="amplification of UPRT/DHFR backbone for generation 
                     of pTUB-FLuc by Gibson assembly.  Use with JBR117"
     primer_bind     complement(7111..7132)
                     /label=JBR110
                     /note="Amplification of UPRT-DHFRflox_AMP_3'UTR backbone 
                     for GA to create FLYLUC expression construct."
     misc_feature    7133..7740
                     /label=a-tubulin promoter
     primer_bind     7179..7202
                     /label=JBR120
                     /note="GA primer set 2 for amp of gblock. use with JBR119 
                     OR 121.  Fuse overlap sequence as in JBR118"
     primer_bind     7605..7625
                     /label=JB114
                     /note="sequencing primer sits at 3' end of pTub for 
                     sequencing across Fluc start site."
     CDS             7754..9394
                     /codon_start=1
                     /label=Firefly luciferase seq
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGK"
     primer_bind     complement(7930..7950)
                     /label=JB115
                     /note="for sequencing across FLUC start of translation in 
                     pTUBFLUC UPRT plasmid.  sits in fluc seq."
     misc_feature    9407..9882
                     /label=DHFR-TS 3'UTR
     primer_bind     9845..9904
                     /label=JBR117
                     /note="amplifcation of UPRT/DHFR backbone for pTUB Fluc 
                     expression construct.  Use with JBR116"
     primer_bind     complement(9859..9918)
                     /label=JBR119
                     /note="GA with gblock. use with JBR118 to amplify Fluc 
                     expression cassette."
     primer_bind     complement(9859..9902)
                     /label=JBR121
                     /note="GA for gblock FLUC.  Use with JBR120.  shortened 
                     primer to avoid self annealing.  Can also use with JBR118 
                     to amplify sequence."
     primer_bind     9884..9904
                     /label=JBR112
                     /note="amplification of UPRT-DHFRflox_AMP-3'UPRT backbone 
                     for GA in building FLUC reporter.  Use with JBR110"
     misc_feature    9903..10782
                     /label=dnUPRT 3' HR Flank
     primer_bind     complement(10745..10764)
                     /label=dnUPRT 2 R
     primer_bind     complement(10765..10782)
                     /label=dnUPRT R
ORIGIN
        1 ttatacatag ttgataattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
       61 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
      121 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc
      181 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact
      241 ctcagtgcaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
      301 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
      361 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
      421 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
      481 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
      541 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
      601 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
      661 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
      721 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
      781 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
      841 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
      901 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
      961 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     1021 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     1081 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     1141 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     1201 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     1261 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     1321 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggt cagatggtaa gccctcccgt
     1381 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     1441 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     1501 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     1561 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     1621 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     1681 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     1741 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     1801 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     1861 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     1921 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     1981 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     2041 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     2101 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     2161 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     2221 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggt cttttgctgg
     2281 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     2341 gcctttgagt gagctgatac cagcggataa caatttcaca caggaaacag ctatgaccat
     2401 gattacgcca agctatcaac tttgtataga aaagttgagc tactcctgtg tctgagtggg
     2461 aattaggtct cccgtatgtt ttccatctct gccccgtcta tatcccctgt ttgtctcctt
     2521 cagtcttcta cgccgaccgc ctgattcgcc tcctcatcga agaagctttg aacggtgagt
     2581 ctcaagtctc aaaagcagat ccgcttctct tgtactgcta gcatgagtct gaaaatgtag
     2641 gcaacgccta aaaagcggtg catgacccac ttcagtctac gtcaaaaaca cgccgctttt
     2701 ttggagagat ggagttaatc ggtttgctga gaaactgtac aatttattta attcgagact
     2761 tggattgttt gacatggcag gaagggtctg agcgtgcagc tgatgcaggc agactctagt
     2821 gagtgtccca acgtcgcaag taactgctgg aagcatgcag ccatatacga aactccgagg
     2881 agtaaattgt gttcttctgt cggtgtctca catgtgtttt ctgtttcgag ggtcaacatg
     2941 tttacatatg tagatatgag tttgaacgcg tgtatcccgc ttcatacgta cgtctgtctc
     3001 tgattgcctt cttcacttct tcatgtctat caacatgtta ctgcgtttgc ggatgcggag
     3061 ttctagactt tcaactgacg tcgggtgcct acgttcttct acattttact gcagaactgc
     3121 cgttcgaaaa gaaggaagtg acaacccctc tggatgtgtc ataccatgga gtttccttct
     3181 attccaagat cgaattcgag ctcggtaccc ataacttcgt atagcataca ttatacgaag
     3241 ttataagctt cgccaggctg taaatcccgt gagtcgtcct cacaaatcat caagcaggtg
     3301 tcctcaggga gactgcctga ctgagttatg ctaattcctt tctactttgg cgtggtcacg
     3361 ggcgcgccgg atccttaatt aagtctagca tgtcattcga ttttcacccc ccgcgtagtt
     3421 cctgtgtgtc attcgttgtc gagacaactc tgtcccgccc cggtgctgtt ccatatgcgt
     3481 gactttcccg caattttttc agactttcag gaaagacagg ctccggaacg atctcgtcca
     3541 tgactggtaa atccacgaca ccgcaatggc ccccagcacc tctatctctc gtgccagggg
     3601 actaacgttg tatgcgtctg cgtcttgtct ttttgcattc gctttccaaa aaagagagcc
     3661 atccgttccc ccgcacattc aacgccgcga gtgcggtttt tgtctttttt gagtggtagg
     3721 acgcttttca tgcgcgaact acgtggacat taagttccat tctctttttc gacagcacga
     3781 aaccttgcat tcaaacccgc ccgcggaaga tccgatcttg ctgctgttcg cagtcccagt
     3841 agcgtcctgt cggccgcgcc gtctctgttg gtgggcagcc gctacacctg ttatctgact
     3901 gccgtgcgcg aaaatgacgc catttttggg aaaatcgggg aacttcattc tttaaaagta
     3961 tgcggaggtt tcctttttct tctgttcgtt tctttttctc gggtttgata accgtgttcg
     4021 atgtaagcac tttccgtctc tcctccgtgc tttgttcgac atcgagacca ggtgtgcaga
     4081 tccttcgctt gtcgatccgg agacgcgtgt ctcgtagaac cttttcattt taccacacgg
     4141 cagtgcggag cactgctctg agtgcagcag ggacgggtga agtttcgctt tagtagtgcg
     4201 tttctgctct acggggcgtt gtcgtgtctg ggaagatgca gaaaccggtg tgtctggtcg
     4261 tcgcgatgac ccccaagagg ggcatcggca tcaacaacgg cctcccgtgg ccccacttga
     4321 ccacagattt caaacacttt cgtcgtgtga caaaaacgac gcccgaagaa gccagtcgcc
     4381 tgaacgggtg gcttcccagg aaatttgcaa agacgggcga ctctggactt ccctctccat
     4441 cagtcggcaa gagattcaac gccgttgtca tgggacggaa aaactgggaa agcatgcctc
     4501 gaaagtttag acccctcgtg gacagattga acatcgtcgt ttcctcttcc ctcaaagaag
     4561 aagacattgc ggcggagaag cctcaagctg aaggccagca gcgcgtccga gtctgtgctt
     4621 cactcccagc agctctcagc cttctggagg aagagtacaa ggattctgtc gaccagattt
     4681 ttgtcgtggg aggagcggga ctgtacgagg cagcgctgtc tctgggcgtt gcctctcacc
     4741 tgtacatcac gcgtgtagcc cgcgagtttc cgtgcgacgt tttcttccct gcgttccccg
     4801 gagatgacat tctttcaaac aaatcaactg ctgcgcaggc tgcagctcct gccgagtctg
     4861 tgttcgttcc cttttgtccg gagctcggaa gagagaagga caatgaagcg acgtatcgac
     4921 ccatcttcat ttccaagacc ttctcagaca acggggtacc ctacgacttt gtggttctcg
     4981 agaagagaag gaagactgac gacgcagcca ctgcggaacc gagcaacgca atgagctcct
     5041 tgacgtccac gagggagaca actcccgtgc acgggttgca ggctccttct tcggccgcag
     5101 ccattgcccc ggtgttggcg tggatggacg aagaagaccg gaaaaaacgc gagcaaaagg
     5161 aactgattcg ggccgttccg catgttcact ttagaggcca tgaagaattc cagtaccttg
     5221 atctcattgc cgacattatt aacaatggaa ggacaatgga tgaccgaacg ggcgttggtg
     5281 tcatctccaa attcggctgc actatgcgct actcgctgga tcaggccttt ccacttctca
     5341 ccacaaagcg tgtgttctgg aaaggggtcc tcgaagagtt gctgtggttc attcgcggcg
     5401 acacgaacgc aaaccatctt tctgagaagg gcgtgaagat ctgggacaag aatgtgacac
     5461 gcgagttcct cgattcgcgc aatctccccc accgagaggt cggagacatc ggcccgggct
     5521 acggcttcca gtggagacac ttcggcgcgg catacaaaga catgcacaca gactacacag
     5581 ggcagggcgt cgaccagctg aagaatgtga tccagatgct gagaacgaat ccaacagatc
     5641 gtcgcatgct catgactgcc tggaatcctg cagcgctgga cgaaatggcg ctgccgcctt
     5701 gtcacttgtt gtgccagttc tacgtgaacg accagaagga gctgtcgtgc atcatgtatc
     5761 agcggtcgtg cgatgtcggc ctcggcgtcc ccttcaacat cgcttcctat tcgcttttga
     5821 cgctcatggt tgcacacgtc tgcaacctaa aacctaagga gttcattcac ttcatgggga
     5881 acacgcatgt ctacacgaac catgtcgagg ctttaaaaga gcagctgcgg agagaaccga
     5941 gaccgttccc cattgtgaac atcctcaaca aggaacgcat caaggaaatc gacgatttca
     6001 ccgccgagga ttttgaggtc gtgggctacg tcccgcacgg acgaatccag atggagatgg
     6061 ctgtctaagc tgagcataac ttcgtatagc atacattata cgaagttatc ggaaatacag
     6121 aagctgcccg tctctcgttt tcctctcttt tcggagggat cagggagagt gcctcgggtc
     6181 ggagagagct gacgaggggg tgccagagac ccctgtgtcc tttatcgaag aaaagggatg
     6241 actcttcatg tggcatttca cacagtctca cctcgccttg ttttcttttt gtcaatcaga
     6301 acgaaagcga gttgcgggtg acgcagatgt gcgtgtatcc actcgtgaat gcgttatcgt
     6361 tctgtatgcc gctagagtgc tggactgttg ctgtctgccc acgacagcag acaactttcc
     6421 ttctatgcac ttgcaggatg gtgcagcgca aacgacggag agaaaggagc accctctcag
     6481 tttccctacg atgtgctgtc agtttcgact cttcaccgcg aacgattggc gatacgtctc
     6541 tgttgacttg ttaggctccg accacgaagc tcccttaact aaataagccg cgacacctaa
     6601 gtgtacacca tttgcagatc gataatctgc gaccgctgaa tccgtccaga tcagtaaaac
     6661 cgcaccacct aagtgtaaac cttgtttagg tcgataaaat gctaccaacc cccacccaca
     6721 atcgagcctt gagcgtttct gcgcacgcgt tggcctacgt gacttgctga tgcctgcctc
     6781 tggccattca tgccagtcag tgcgcataaa aatgtggaca cagtcggttg acaagtgttc
     6841 tggcaggcta cagtgacacg gggaaatcgt ttctgagaga gcaccaaaac cttttagctg
     6901 tcaccatctt cttcgcttgc tacgttgtct gatttgtcgt ttttcctctc ggtgtgaagc
     6961 gtttttcgta acgtcaatcg acccaggaca ctgcttccag ctgtctgtgc cgtcctgtcg
     7021 tgaccagttt gtcgccgcga ctttatgagc tttttcctgt tgagaaagcg gttcggcggc
     7081 cgccacgtga catgcgggag aagaaaacgt gaccgaacaa atcaactggt gagaagagtg
     7141 tgttttcgtt taattcgtca cagaccacga aaaacgaacc atcgaagacg atcactgcgt
     7201 ccgcgtgcat ctggatggat gacccacatc tgttgcagcc gtcgcagaca tgcatgtccc
     7261 gcgttcgtga aattctctgc atcagcggag tgatcaggaa tcatcgtctc agcgggatga
     7321 cgttgcggag caggccggct cgcggtgggc agtcagatgc cgaaggcgta actcaggacg
     7381 gcttgcgctc atcgcagaac aggggtggtg cctgcattgg gtgcggttgg tgatcctggt
     7441 tggaccggtg gagatgcgcg cgcacgaagg ggatgtgtca gaaacatttt gtttgttctc
     7501 tgtgaacttt tagatgtgtt aaaggcggcg aatattagca gagagtcctc cttgttggat
     7561 tctctcttga atttcgccct ttccttctct ttgcgagtct cgtagagaac aagcactcgt
     7621 tcgccgtccc tgacgacgca acccgcgcag aagacatcca ccaaacggtg ttacacaatc
     7681 accttgtgtg aagttcttgc ggaaaactac tcgttggcat tttttcttga attccctttt
     7741 agatctggca aaaatggaag acgccaaaaa cataaagaaa ggcccggcgc cattctatcc
     7801 tctagaggat ggaaccgctg gagagcaact gcataaggct atgaagagat acgccctggt
     7861 tcctggaaca attgctttta cagatgcaca tatcgaggtg aacatcacgt acgcggaata
     7921 cttcgaaatg tccgttcggt tggcagaagc tatgaaacga tatgggctga atacaaatca
     7981 cagaatcgtc gtatgcagtg aaaactctct tcaattcttt atgccggtgt tgggcgcgtt
     8041 atttatcgga gttgcagttg cgcccgcgaa cgacatttat aatgaacgtg aattgctcaa
     8101 cagtatgaac atttcgcagc ctaccgtagt gtttgtttcc aaaaaggggt tgcaaaaaat
     8161 tttgaacgtg caaaaaaaat taccaataat ccagaaaatt attatcatgg attctaaaac
     8221 ggattaccag ggatttcagt cgatgtacac gttcgtcaca tctcatctac ctcccggttt
     8281 taatgaatac gattttgtac cagagtcctt tgatcgtgac aaaacaattg cactgataat
     8341 gaattcctct ggatctactg ggttacctaa gggtgtggcc cttccgcata gaactgcctg
     8401 cgtcagattc tcgcatgcca gagatcctat ttttggcaat caaatcattc cggatactgc
     8461 gattttaagt gttgttccat tccatcacgg ttttggaatg tttactacac tcggatattt
     8521 gatatgtgga tttcgagtcg tcttaatgta tagatttgaa gaagagctgt ttttacgatc
     8581 ccttcaggat tacaaaattc aaagtgcgtt gctagtacca accctatttt cattcttcgc
     8641 caaaagcact ctgattgaca aatacgattt atctaattta cacgaaattg cttctggggg
     8701 cgcacctctt tcgaaagaag tcggggaagc ggttgcaaaa cgcttccatc ttccagggat
     8761 acgacaagga tatgggctca ctgagactac atcagctatt ctgattacac ccgaggggga
     8821 tgataaaccg ggcgcggtcg gtaaagttgt tccatttttt gaagcgaagg ttgtggatct
     8881 ggataccggg aaaacgctgg gcgttaatca gagaggcgaa ttatgtgtca gaggacctat
     8941 gattatgtcc ggttatgtaa acaatccgga agcgaccaac gccttgattg acaaggatgg
     9001 atggctacat tctggagaca tagcttactg ggacgaagac gaacacttct tcatagttga
     9061 ccgcttgaag tctttaatta aatacaaagg atatcaggtg gcccccgctg aattggaatc
     9121 gatattgtta caacacccca acatcttcga cgcgggcgtg gcaggtcttc ccgacgatga
     9181 cgccggtgaa cttcccgccg ccgttgttgt tttggagcac ggaaagacga tgacggaaaa
     9241 agagatcgtg gattacgtcg ccagtcaagt aacaaccgcg aaaaagttgc gcggaggagt
     9301 tgtgtttgtg gacgaagtac cgaaaggtct taccggaaaa ctcgacgcaa gaaaaatcag
     9361 agagatcctc ataaaggcca agaagggcgg aaagtaactg cagcccacag aagctgcccg
     9421 tctctcgttt tcctctcttt tcggagggat cagggagagt gcctcgggtc ggagagagct
     9481 gacgaggggg tgccagagac ccctgtgtcc tttatcgaag aaaagggatg actcttcatg
     9541 tggcatttca cacagtctca cctcgccttg ttttcttttt gtcaatcaga acgaaagcga
     9601 gttgcgggtg acgcagatgt gcgtgtatcc actcgtgaat gcgttatcgt tctgtatgcc
     9661 gctagagtgc tggactgttg ctgtctgccc acgacagcag acaactttcc ttctatgcac
     9721 ttgcaggatg gtgcagcgca aacgacggag agaaaggagc accctctcag tttccctacg
     9781 atgtgctgtc agtttcgact cttcaccgcg aacgattggc gatacgtctc tgttgacttg
     9841 ttaggctccg accacgaagc tcccttaact agataagccg cgtctagagt cgacctgcag
     9901 gcgcgagtcg atggaaagcg gcttgcgggc agtttgccgc ggctgccgca tcgggaaaat
     9961 cctcatccag agagacgaaa caactgcgga gcctaaggta cggacttgtt tgcgttctcg
    10021 ctacctgggg accacagaca tcaggtgtct tcgagtgcag ctgtcgctgg aatttcccct
    10081 cctgtcgttc accgcgagaa aaagctaagg aagagggaga aggtgaagag cgataggcaa
    10141 cccaagaggc tgctgtccac tttgttacac taaaacagat tggagtataa gaagcacaac
    10201 ttggcctgaa acacagaaca gagcggagag actacgcagt tgtgtctgtg gaggaagagc
    10261 acaattctgt tgtgacggga aggaagaaga gagagggact cgtaggtcag ccggaaggga
    10321 aggcaggtcg tggaaactat ggaggcaaga ggaaagaatt gctgcgctct gactcactgc
    10381 acgtgttttc cttacccatt gcctcccatt ttctgacacg gatgatattc atcgctgaac
    10441 ttgacagttg gttgtggctg tttgttgctg gtttgcgtgg ccaagaccgc cgtgggcgcc
    10501 cctctgctct tgtccgctgc acctgcttcc tccgcccttg atgtttgctc tgttccagct
    10561 gatctacgag aagctgcctg ccgacattag agatcgctgg gtgatgctgc tcgatccgat
    10621 gtgcgcgacg gcgggcagtg tgtgcaaagc gatcgaggtc ctcctgaggc tcggcgtgaa
    10681 ggaagagaga atcattttcg tcaacatttt ggctgctccc caaggcattg aacgtgtttt
    10741 caaggaatac ccgaaagtcc gcatggtcac tgctgcgtaa gcaacttta
//
