LOCUS       Otop1_pGEMHE            4822 bp    DNA     circular      9-SEP-2016
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11254408|
COMMENT     VNTDBDATE|-11407365|
COMMENT     LSOWNER|
COMMENT     VNTNAME|Otop1_pGEMHE|
COMMENT     VNTAUTHORNAME|Liman Lab|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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FEATURES             Location/Qualifiers
     promoter        547..566
                     /vntifkey="29"
                     /label=T7_promoter
     promoter        complement(2710..2728)
                     /vntifkey="29"
                     /label=Sp6_promoter
     CDS             complement(3907..4767)
                     /vntifkey="4"
                     /label=Amp
     CDS             668..2470
                     /vntifkey="4"
                     /label=Otop1
     misc_feature    1854..1854
                     /vntifkey="21"
                     /label=G-->A\(SNP)
BASE COUNT     1120 a      1288 c      1251 g      1163 t 
ORIGIN
        1 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 
       61 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat 
      121 aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga 
      181 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa 
      241 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca 
      301 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta 
      361 aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg 
      421 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg 
      481 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt 
      541 gaattgtaat acgactcact atagggcgaa ttaattcgag ctcggtaccc agcttgcttg 
      601 ttctttttgc agaagctcag aataaacgct caactttggc agatcaattc cccggggatc 
      661 cgaattcatg cctgggggcc cgggcgcgcc ctcttctccc gcagcatcct caggctcctc 
      721 gcgtgccgcg ccgtcgggga tcgcggcatg ccctctgtcc ccacctcccc tagcccgagg 
      781 gtctccacaa gcttccggtc cccggcgcgg cgccagcgtc ccgcagaagc tggcggagac 
      841 tctgagcagc cagtatggac tgaacgtgtt cgtggcgggg ctgctgttcc tgctggcctg 
      901 ggcggtgcac gcgacgggtg tgggcaagag cgacctgctg tgcgttctaa ccgcgcttat 
      961 gctgctgcag ctgctctgga tgctgtggta cgtgggtcgc agctacatgc agcgccgcct 
     1021 catccgcccc aaggacacgc acgcgggtgc gcgctggctt cgcggaagca tcacgttgtt 
     1081 cgcgtttatc actgtcgtcc tgggatgctt gaaagtcgca tacttcattg gattctcgga 
     1141 gtgcttgtca gccaccgagg gagttttccc agtcacccat gcagtgcata ccctattgca 
     1201 ggtgtatttc ctctggggcc atgctaagga tatcatcatg tctttcaaaa cactggaaag 
     1261 gtttggggtg atccattcag tgttcacgaa cctcctactg tgggccaaca gcgtcctgaa 
     1321 tgaatcaaag caccagctga atgagcacaa ggaacggctg atcactctgg gctttggcaa 
     1381 catcaccatc gttttggatg accacacacc acagtgtaac tgcacaccac ccgccctctg 
     1441 ctctgccctc tcccatggga tttactatct gtaccccttc aacattgagt accagatcct 
     1501 ggcctcgacc atgctctacg tgctgtggaa gaacatcggg cgcagagtgg acagctccca 
     1561 gcaccagaag atgcagtgca gattcgacgg ggtcctagtg ggctccgtgc tgggcttgac 
     1621 agtgctggct gccaccatcg ccgtggttgt ggtgtacatg atccacatcg ggcgctccaa 
     1681 atccaagagc gagtcggctc tcatcatgtt ctatttgtac gctatcacgg tgctgctgct 
     1741 tatgggggcc gcagggctag tcggaagctg gatttacagg gtggatgaga agtctctgga 
     1801 tgagtcaaag aacccagcgc gcaagctgga tgttgacctg ttggtggcca ccggctccgg 
     1861 ctcctggctc ctctcctggg gctccattct ggccatcgcc tgtgctgaga ctcgcccacc 
     1921 gtacacctgg tacaacctgc cctactcggt cctggtgatc gtggagaagt atgtccaaaa 
     1981 cattttcatc atcgagtccg tgcacctcga gcctgagggg gtcccggagg atgtgcgcac 
     2041 tctgcgtgtg gtcaccgtct gcagcagcga ggctgccgca ctggctgcat ccactctcgg 
     2101 gagccagggg atggcccagg atgggtcacc tgctgtcaat ggaaatctgt gtctgcagca 
     2161 gaggtgtggg aaagaggacc aggagtctgg ctgggaagga gctacgggga caacccgatg 
     2221 tctggacttc cttcagggcg gcatgaagag gaggcttctc agaaacatca cggcctttct 
     2281 gtttctttgc aacatctcgc tttggattcc ccctgccttt ggctgccgtc ccgagtatga 
     2341 caacggattg gaggaaattg tctttggctt tgaaccctgg ataattgtgg tcaacctggc 
     2401 catgcccttt tccattttct accggatgca cgcagctgct gccctctttg aggtctattg 
     2461 taagatctag tctagagcaa gcttgatctg gttaccacta aaccagcctc aagaacaccc 
     2521 gaatggagtc tctaagctac ataataccaa cttacacttt acaaaatgtt gtcccccaaa 
     2581 atgtagccat tcgtatctgc tcctaataaa aagaaagttt cttcacattc taaaaaaaaa 
     2641 aaaaaaaaaa aaaaaaaaaa aaaacccccc cccccccccc cctgcaggca tgcaagctag 
     2701 cttgagtatt ctatagtgtc acctaaatag cttggcgtaa tcatggtcat agctgtttcc 
     2761 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg 
     2821 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc 
     2881 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg 
     2941 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 
     3001 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 
     3061 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 
     3121 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 
     3181 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 
     3241 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 
     3301 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 
     3361 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 
     3421 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 
     3481 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 
     3541 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg 
     3601 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 
     3661 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 
     3721 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 
     3781 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 
     3841 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 
     3901 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 
     3961 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 
     4021 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 
     4081 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 
     4141 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 
     4201 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc 
     4261 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 
     4321 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 
     4381 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 
     4441 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 
     4501 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa 
     4561 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 
     4621 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 
     4681 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 
     4741 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 
     4801 tcagggttat tgtctcatga gc 
//
