LOCUS       Otop3_X1_pGEMHE         4753 bp    DNA     circular      5-JUN-2017
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11286203|
COMMENT     VNTDBDATE|-11794379|
COMMENT     LSOWNER|
COMMENT     VNTNAME|Otop3_X1_pGEMHE|
COMMENT     VNTAUTHORNAME|Liman Lab|
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FEATURES             Location/Qualifiers
     promoter        547..566
                     /vntifkey="29"
                     /label=T7_promoter
     promoter        complement(2641..2659)
                     /vntifkey="29"
                     /label=Sp6_promoter
     primer          124..143
                     /vntifkey="27"
                     /label=ExpLib_IVT_F
     CDS             complement(3838..4698)
                     /vntifkey="4"
                     /label=Amp
     CDS             668..2401
                     /vntifkey="4"
                     /label=Otop3_transcript\variant\2
BASE COUNT     1124 a      1281 c      1202 g      1146 t 
ORIGIN
        1 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 
       61 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat 
      121 aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga 
      181 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa 
      241 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca 
      301 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta 
      361 aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg 
      421 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg 
      481 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt 
      541 gaattgtaat acgactcact atagggcgaa ttaattcgag ctcggtaccc agcttgcttg 
      601 ttctttttgc agaagctcag aataaacgct caactttggc agatcaattc cccggggatc 
      661 cgaattcatg gcttcgcaga cttcagcccc tgctgagcct gcacccatgc cttctccaga 
      721 agcaaagacc actgagggag catcctccta tgaccaagca gacatggaga ctaagcatgc 
      781 ggggtcccca tgccccccga agcagaagtc ctggctggct cggcatttct ccctgctgtt 
      841 gcggagagac cgacaggccc agaaggccgg gcagctcttc tccgggctcc tggctctcaa 
      901 tgtggtattc ctgggtgggg cttttatctg tagcatgata ttcaacaagg tgtctgtcac 
      961 tctgggggac gtgtggatcc tgctggctgc actgaaggtc ctctccctgt tgtggctcct 
     1021 ctactacaca gtgggtacca cccggaagcc ccacgcagtc ctgtaccgtg atccccacgc 
     1081 gggacccatc tgggtgaggg gctccctggt gctcttcggc agctgtaccg tctgcctgaa 
     1141 catatttcga atgggttatg acgtgagcca cattcactgc aagtcagaag tggaactcat 
     1201 ctttccggcc attgagattg tctttatgat tattcagaca tgggtgctct ggagacactg 
     1261 taaggactgt gttcaggtgc agaccaactt caccaggtgt ggactcatgc tgactctggc 
     1321 cacaaacttg ctgatgtggg ttttggctgt gaccaatgac tccatgcacc gtgagattga 
     1381 ggctgagctc gacgccctca tggagaagtt ctcaggtaac gggaccaaca cttgcatgtg 
     1441 cctcaacacc acagtgtgcg aggtcttccg gaagggctat ctgatgctct accccttcag 
     1501 cactgagtac tgcctcatct gctgtgctgt gctcttcgtc atgtggaaga acgtgagccg 
     1561 cagcctggca gcccacacag gtgcccatcc caacagatca cccttccgcc tgcacgggac 
     1621 catctttggg ccactgctgg gccttttggc actggtggct ggcgtatgtg tctttgtgct 
     1681 cttccaaatc gaagcgagtg gccctgacat agcccgccag tacttcaccc tctactatgc 
     1741 gttctatgtg gctgtgctgc ccaccatgag cctggcgtgc ctggctggta cagccatcca 
     1801 tgggctggag gagcgcgaat tggacacgct caaaaacccc acacgcagcc tggatgtggt 
     1861 gctgttgatg ggcgctgcac tgggccagat gggcatcgcc tacttctcca tcgtggctat 
     1921 cgtggccacc cagccgcacg agctactcaa ccagcttatc ctggcctact cactgctgct 
     1981 aatcctgcag cacatcactc agaatctctt catcatcgag ggcctgcacc gccgccccct 
     2041 ctgggagcct gcggtctcag gtgtgatgga gaagcaggat gttgagctac cccgcagagg 
     2101 ctccctgcgg gagctgggtc aggacctacg gagagcctca agggcctaca tccactcctt 
     2161 cagccacctc aactggaaac gcaggatgct caaggagatc tctctcttcc tcatcctctg 
     2221 caacatcaca ctgtggatga tgcccgcctt cggcatacac ccagagtttg agaacgggct 
     2281 ggaaaaggat ttttacggct acaggacctg gttcaccatt gttaacttcg gcctgcccct 
     2341 gggtgtgttc taccgcatgc actctgtcgg ggggctggtg gaggtctacc tgggggcctg 
     2401 atctagagca agcttgatct ggttaccact aaaccagcct caagaacacc cgaatggagt 
     2461 ctctaagcta cataatacca acttacactt tacaaaatgt tgtcccccaa aatgtagcca 
     2521 ttcgtatctg ctcctaataa aaagaaagtt tcttcacatt ctaaaaaaaa aaaaaaaaaa 
     2581 aaaaaaaaaa aaaaaccccc cccccccccc ccctgcaggc atgcaagcta gcttgagtat 
     2641 tctatagtgt cacctaaata gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa 
     2701 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg 
     2761 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca 
     2821 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg 
     2881 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 
     2941 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 
     3001 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 
     3061 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 
     3121 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 
     3181 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 
     3241 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 
     3301 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 
     3361 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 
     3421 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 
     3481 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc 
     3541 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 
     3601 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 
     3661 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 
     3721 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 
     3781 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 
     3841 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 
     3901 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 
     3961 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 
     4021 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 
     4081 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 
     4141 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 
     4201 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 
     4261 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 
     4321 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 
     4381 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 
     4441 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat 
     4501 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 
     4561 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 
     4621 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 
     4681 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 
     4741 ttgtctcatg agc 
//
