LOCUS       Otop1_pCDNA3            7232 bp    DNA     circular     13-APR-2018
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11270337|
COMMENT     VNTDBDATE|-12243649|
COMMENT     LSOWNER|
COMMENT     VNTNAME|Otop1_pCDNA3|
COMMENT     VNTAUTHORNAME|Liman Lab|
COMMENT     VNTOAUTHORNAME|Emily Liman|
COMMENT     VNTOAUTHOREML|liman@usc.edu|
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COMMENT                       "Currently local object. Original author: Emily Liman" 1) 
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COMMENT                       "Created: 06/06/16 02:12PM" 1) 
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FEATURES             Location/Qualifiers
     promoter        7207..7226
                     /vntifkey="30"
                     /label=T7
     promoter        complement(1897..1914)
                     /vntifkey="30"
                     /label=Sp6
     misc_feature    56..58
                     /vntifkey="21"
                     /label=KOZAK
     CDS             59..1861
                     /vntifkey="4"
                     /label=Otop1
BASE COUNT     1580 a      1942 c      1911 g      1799 t 
ORIGIN
        1 aagcttggta ccgagctcgg atccactagt aacggccgcc agtgtgctgg aattcaccat 
       61 gcctgggggc ccgggcgcgc cctcttctcc cgcagcatcc tcaggctcct cgcgtgccgc 
      121 gccgtcgggg atcgcggcat gccctctgtc cccacctccc ctagcccgag ggtctccaca 
      181 agcttccggt ccccggcgcg gcgccagcgt cccgcagaag ctggcggaga ctctgagcag 
      241 ccagtatgga ctgaacgtgt tcgtggcggg gctgctgttc ctgctggcct gggcggtgca 
      301 cgcgacgggt gtgggcaaga gcgacctgct gtgcgttcta accgcgctta tgctgctgca 
      361 gctgctctgg atgctgtggt acgtgggtcg cagctacatg cagcgccgcc tcatccgccc 
      421 caaggacacg cacgcgggtg cgcgctggct tcgcggaagc atcacgttgt tcgcgtttat 
      481 cactgtcgtc ctgggatgct tgaaagtcgc atacttcatt ggattctcgg agtgcttgtc 
      541 agccaccgag ggagttttcc cagtcaccca tgcagtgcat accctattgc aggtgtattt 
      601 cctctggggc catgctaagg atatcatcat gtctttcaaa acactggaaa ggtttggggt 
      661 gatccattca gtgttcacga acctcctact gtgggccaac agcgtcctga atgaatcaaa 
      721 gcaccagctg aatgagcaca aggaacggct gatcactctg ggctttggca acatcaccat 
      781 cgttttggat gaccacacac cacagtgtaa ctgcacacca cccgccctct gctctgccct 
      841 ctcccatggg atttactatc tgtacccctt caacattgag taccagatcc tggcctcgac 
      901 catgctctac gtgctgtgga agaacatcgg gcgcagagtg gacagctccc agcaccagaa 
      961 gatgcagtgc agattcgacg gggtcctagt gggctccgtg ctgggcttga cagtgctggc 
     1021 tgccaccatc gccgtggttg tggtgtacat gatccacatc gggcgctcca aatccaagag 
     1081 cgagtcggct ctcatcatgt tctatttgta cgctatcacg gtgctgctgc ttatgggggc 
     1141 cgcagggcta gtcggaagct ggatttacag ggtggatgag aagtctctgg atgagtcaaa 
     1201 gaacccagcg cgcaagctgg atgttgacct gttggtggcc accggctccg gctcctggct 
     1261 cctctcctgg ggctccattc tggccatcgc ctgtgctgag actcgcccac cgtacacctg 
     1321 gtacaacctg ccctactcgg tcctggtgat cgtggagaag tatgtccaaa acattttcat 
     1381 catcgagtcc gtgcacctcg agcctgaggg ggtcccggag gatgtgcgca ctctgcgtgt 
     1441 ggtcaccgtc tgcagcagcg aggctgccgc actggctgca tccactctcg ggagccaggg 
     1501 gatggcccag gatgggtcac ctgctgtcaa tggaaatctg tgtctgcagc agaggtgtgg 
     1561 gaaagaggac caggagtctg gctgggaagg agctacgggg acaacccgat gtctggactt 
     1621 ccttcagggc ggcatgaaga ggaggcttct cagaaacatc acggcctttc tgtttctttg 
     1681 caacatctcg ctttggattc cccctgcctt tggctgccgt cccgagtatg acaacggatt 
     1741 ggaggaaatt gtctttggct ttgaaccctg gataattgtg gtcaacctgg ccatgccctt 
     1801 ttccattttc taccggatgc acgcagctgc tgccctcttt gaggtctatt gtaagatcta 
     1861 ggcggccgct cgagcatgca tctagagggc cctattctat agtgtcacct aaatgctaga 
     1921 gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc 
     1981 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag 
     2041 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag 
     2101 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct 
     2161 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt 
     2221 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc 
     2281 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc 
     2341 tttccccgtc aagctctaaa tcggggcatc cctttagggt tccgatttag tgctttacgg 
     2401 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga 
     2461 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc 
     2521 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg 
     2581 gggatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa 
     2641 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca ggcaggcaga 
     2701 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc 
     2761 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc 
     2821 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc 
     2881 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag 
     2941 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt 
     3001 atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa 
     3061 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg 
     3121 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc 
     3181 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca 
     3241 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc 
     3301 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca 
     3361 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat 
     3421 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca 
     3481 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg 
     3541 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc 
     3601 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct 
     3661 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct 
     3721 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac 
     3781 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc 
     3841 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag 
     3901 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg 
     3961 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact 
     4021 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata 
     4081 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc 
     4141 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc 
     4201 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt 
     4261 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc 
     4321 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg 
     4381 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct 
     4441 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 
     4501 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga 
     4561 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 
     4621 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 
     4681 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 
     4741 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt 
     4801 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 
     4861 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 
     4921 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 
     4981 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg 
     5041 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 
     5101 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 
     5161 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 
     5221 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 
     5281 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 
     5341 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 
     5401 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 
     5461 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 
     5521 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 
     5581 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 
     5641 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg 
     5701 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 
     5761 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 
     5821 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 
     5881 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 
     5941 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa 
     6001 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 
     6061 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 
     6121 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 
     6181 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 
     6241 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 
     6301 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc 
     6361 tcccgatccc ctatggtcga ctctcagtac aatctgctct gatgccgcat agttaagcca 
     6421 gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc 
     6481 tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt 
     6541 tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta 
     6601 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac 
     6661 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc 
     6721 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt 
     6781 ggactattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac 
     6841 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac 
     6901 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt 
     6961 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc 
     7021 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt 
     7081 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg 
     7141 ggaggtctat ataagcagag ctctctggct aactagagaa cccactgctt actggcttat 
     7201 cgaaattaat acgactcact atagggagac cc 
//
