LOCUS New_DNA 2874 bp ds-DNA circular 15-MAY-2017 DEFINITION Cloning vector pUC19c, complete sequence. ACCESSION L09137 X02514 VERSION L09137.2 GI:20141090 KEYWORDS . SOURCE Cloning vector pUC19c ORGANISM Cloning vector pUC19c other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 2686) AUTHORS Yanisch-Perron,C., Vieira,J. and Messing,J. TITLE Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors JOURNAL Gene 33 (1), 103-119 (1985) PUBMED 2985470 REFERENCE 2 (bases 1 to 2686) AUTHORS Chambers,S.P., Prior,S.E., Barstow,D.A. and Minton,N.P. TITLE The pMTL nic- cloning vectors. I. Improved pUC polylinker regions to facilitate the use of sonicated DNA for nucleotide sequencing JOURNAL Gene 68 (1), 139-149 (1988) PUBMED 2851488 REFERENCE 3 (bases 1 to 2686) AUTHORS Gilbert,W. TITLE Obtained from VecBase 3.0 JOURNAL Unpublished REFERENCE 4 (bases 1 to 2686) AUTHORS Messing,J. TITLE Direct Submission JOURNAL Submitted (27-APR-1993) Department of Biochemistry, University of Minnesota, St. Paul, MN 55108, USA REFERENCE 5 (bases 1 to 2686) AUTHORS Messing,J. TITLE Direct Submission JOURNAL Submitted (11-APR-2002) Rutgers, The State University of New Jersey, Waksman Institute of Microbiology, 190 Frelinghuysen Road, Piscataway, NJ 08854-8020, USA REMARK Sequence update by submitter COMMENT pUC19_RE28_C5 from 1 to 2874 COMMENT RE28_C-5; DNASeq# 2754373 COMMENT Maxiprep RE34_RE28_C-5; DNASeq# 2761959 COMMENT COMMENT COMMENT COMMENT COMMENT On Apr 11, 2002 this sequence version replaced gi:209213. These data and their annotation were supplied to GenBank by Will Gilbert under the auspices of the GenBank Currator Program. pUC19c - Cloning vector (beta-galactosidase mRNA on complementary strand) ENTRY PUC19C #TYPE DNA CIRCULAR TITLE pUC19c - Cloning vector (beta-galactosidase mRNA on complementary strand) DATE 03-FEB-1986 #sequence 16-DEC-1986 ACCESSION VB0033 SOURCE artificial COLLECTION ATCC 37254 REFERENCE #number #authors Norrander J., Kempe T., Messing J. #journal Gene (1983) 26: 101-106 REFERENCE #number 1 #authors Yanisch-Perron C., Vieira J., Messing J. #journal Gene (1985) 33: 103-119 #comment shows the complete compiled sequence REFERENCE #number 2 #authors Chambers,S.P., et al. #journal Gene (1988) 68: 139-149 #describes mutation at nt1308 and its effect on copy number REFERENCE #number #authors Pouwels P.H., Enger-Valk B.E., Brammar W.J. #book Cloning Vectors, Elsvier 1985 and supplements #comment vector I-A-iv-20 COMMENT This Sequence was obtained 3-MAR-1986 from J. Messing, Waksman Institute, NJ on floppy disk. Revised 16-DEC-1986 by F. Pfeiffer: 1062/3 'AT' to 'TA' to match revised sequence of PBR322 The beta-galactosidase mRNA sequence including the multiple cloning site of M13mp19 is on the strand complementary to that shown. KEYWORDS CROSSREFERENCE #complement VecBase(3):pUC19 #prerevised GenBank(50):M11662, EMBL(11):ARPuc19 #parent VecBase(3):pUC13, VecBase(3):M13mp19, VecSource(3):bGal19 PARENT Features of pUC19c (2686 bp) residue source 1- 137 2074-2210 pBR322 138- 237 2252-2351 pBR322 238- 395 1461-1304 (c) Lac-Operon 396- 452 57- 1 (c) polylinker of M13mp19 455- 682 1298-1071 (c) Lac-Operon 683-2686 2352-4355 pBR322 Conflict (cfl) and Mutations (mut): pUC19c source mut 1308 A G 2977 pBR322 linked to increased copy number mut 1942 A G 3611 pBR322 mut 2243 T C 3912 pBR322 FEATURE 1629-2417 789-1 (c) Ap-R; b-lactamase POLYLINKER HindIII-SphI-PstI-SalI-XbaI-BamHI-SmaI-KpnI-SacI-EcoRI SELECTION #resistance Ap #indicator beta-galactosidase SUMMARY pUC19c #length 2686 #checksum 4465. COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers source join(1..1569,1758..2874) /organism="Cloning vector pUC19c" /mol_type="genomic DNA" /db_xref="taxon:174689" /label=source:Cloning vector pUC19c /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind complement(1807..1824) /label=M13-rev (DNA Core) /ApEinfo_fwdcolor="#ff8000" /ApEinfo_revcolor="#800080" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" rep_origin complement(2192..2874) /label=ColE1 origin /ApEinfo_fwdcolor="gray50" /ApEinfo_revcolor="gray50" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_binding complement(1830..1852) /label=LacO /ApEinfo_fwdcolor="#6495ed" /ApEinfo_revcolor="#6495ed" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind complement(1184..1206) /label=pGEX 3 primer /ApEinfo_fwdcolor="#ff8000" /ApEinfo_revcolor="#800080" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind 1186..1203 /label=pGEX3_01R /ApEinfo_fwdcolor="#ff8000" /ApEinfo_revcolor="#800080" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind complement(1838..1855) /label=LacO_01F /ApEinfo_fwdcolor="#ff8000" /ApEinfo_revcolor="#800080" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature complement(1758..1806) /label=LacZ alpha tln start- MCS for blue/white screen /ApEinfo_fwdcolor="#8080ff" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind 1533..1550 /label=M13-fwd /ApEinfo_fwdcolor="#ff8000" /ApEinfo_revcolor="#800080" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind 1516..1533 /label=pUC19_Univ_01F /ApEinfo_fwdcolor="#ff8000" /ApEinfo_revcolor="#800080" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" CDS complement(1394..1462) /label=LacZ alpha /ApEinfo_fwdcolor="#6495ed" /ApEinfo_revcolor="#6495ed" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" CDS complement(98..757) /label=AmpR /ApEinfo_fwdcolor="yellow" /ApEinfo_revcolor="yellow" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind 919..936 /label=Amp_01R /ApEinfo_fwdcolor="#ff8000" /ApEinfo_revcolor="#800080" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature complement(1463..1569) /label=LacZ alpha tln start- MCS for blue/white screen(1) /ApEinfo_label="LacZ alpha tln start- MCS for blue/white screen" /ApEinfo_fwdcolor="#8080ff" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" repeat_unit 1570..1616 /label=T790M_28bp /ApEinfo_fwdcolor="#00ffff" /ApEinfo_revcolor="#408080" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" repeat_unit 1617..1663 /label=T790M_28bp(1) /ApEinfo_label="T790M_28bp" /ApEinfo_fwdcolor="#00ffff" /ApEinfo_revcolor="#408080" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" repeat_unit 1664..1710 /label=T790M_28bp(2) /ApEinfo_label="T790M_28bp" /ApEinfo_fwdcolor="#00ffff" /ApEinfo_revcolor="#408080" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" repeat_unit 1711..1757 /label=T790M_28bp(3) /ApEinfo_label="T790M_28bp" /ApEinfo_fwdcolor="#00ffff" /ApEinfo_revcolor="#408080" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" variation 1587..1587 /label=2369C>T, T7790M /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#ff00ff" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" variation 1634..1634 /label=2369C>T, T7790M(1) /ApEinfo_label="2369C>T, T7790M" /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#ff00ff" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" variation 1681..1681 /label=2369C>T, T790M /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#ff00ff" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" variation 1728..1728 /label=2369C>T, T790M(1) /ApEinfo_label="2369C>T, T790M" /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#ff00ff" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" ORIGIN 1 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 61 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 121 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 181 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 241 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 301 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 361 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 421 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 481 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 541 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 601 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 661 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 721 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 781 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 841 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 901 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 961 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 1021 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 1081 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca 1141 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc 1201 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg 1261 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga 1321 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat 1381 accgcatcag gcgCCATTCG CCATTCAGGC TGCGCAACTG TTGGGAAGGG CGATCGGTGC 1441 GGGCCTCTTC GCTATTACGC CAGCTGGCGA AAGGGGGATG TGCTGCAAGG CGATTAAGTT 1501 GGGTAACGCC AGGGTTTTCC CAGTCACGAC GTTGTAAAAC GACGGCCAGT GAATTCGAGC 1561 TCGGTACCCA GCGAGTCATT CAATCATGCA GCTCATGCCC TTCGTAGGAC GTCAAAGTCG 1621 AGTCCGACAA TCATGCAGCT CATGCCCTTC GTAGGACGTC AAGTTGGAGT CAGTCAATCA 1681 TGCAGCTCAT GCCCTTCGTA GGACGTCAAA GTCGAGTCCG ACAATCATGC AGCTCATGCC 1741 CTTCGTAGGA CGTCTAAGGG GATCCTCTAG AGTCGACCTG CAGGCATGCA AGCTTGGCGT 1801 AATCATggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca 1861 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat 1921 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt 1981 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct 2041 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 2101 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 2161 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 2221 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 2281 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 2341 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 2401 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 2461 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 2521 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 2581 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 2641 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 2701 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 2761 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 2821 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atga //