LOCUS New_DNA 2686 bp ds-DNA circular 28-APR-2017 DEFINITION Cloning vector pUC19c, complete sequence. ACCESSION L09137 X02514 VERSION L09137.2 GI:20141090 KEYWORDS . SOURCE Cloning vector pUC19c ORGANISM Cloning vector pUC19c other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 2686) AUTHORS Yanisch-Perron,C., Vieira,J. and Messing,J. TITLE Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors JOURNAL Gene 33 (1), 103-119 (1985) PUBMED 2985470 REFERENCE 2 (bases 1 to 2686) AUTHORS Chambers,S.P., Prior,S.E., Barstow,D.A. and Minton,N.P. TITLE The pMTL nic- cloning vectors. I. Improved pUC polylinker regions to facilitate the use of sonicated DNA for nucleotide sequencing JOURNAL Gene 68 (1), 139-149 (1988) PUBMED 2851488 REFERENCE 3 (bases 1 to 2686) AUTHORS Gilbert,W. TITLE Obtained from VecBase 3.0 JOURNAL Unpublished REFERENCE 4 (bases 1 to 2686) AUTHORS Messing,J. TITLE Direct Submission JOURNAL Submitted (27-APR-1993) Department of Biochemistry, University of Minnesota, St. Paul, MN 55108, USA REFERENCE 5 (bases 1 to 2686) AUTHORS Messing,J. TITLE Direct Submission JOURNAL Submitted (11-APR-2002) Rutgers, The State University of New Jersey, Waksman Institute of Microbiology, 190 Frelinghuysen Road, Piscataway, NJ 08854-8020, USA REMARK Sequence update by submitter COMMENT COMMENT On Apr 11, 2002 this sequence version replaced gi:209213. These data and their annotation were supplied to GenBank by Will Gilbert under the auspices of the GenBank Currator Program. pUC19c - Cloning vector (beta-galactosidase mRNA on complementary strand) ENTRY PUC19C #TYPE DNA CIRCULAR TITLE pUC19c - Cloning vector (beta-galactosidase mRNA on complementary strand) DATE 03-FEB-1986 #sequence 16-DEC-1986 ACCESSION VB0033 SOURCE artificial COLLECTION ATCC 37254 REFERENCE #number #authors Norrander J., Kempe T., Messing J. #journal Gene (1983) 26: 101-106 REFERENCE #number 1 #authors Yanisch-Perron C., Vieira J., Messing J. #journal Gene (1985) 33: 103-119 #comment shows the complete compiled sequence REFERENCE #number 2 #authors Chambers,S.P., et al. #journal Gene (1988) 68: 139-149 #describes mutation at nt1308 and its effect on copy number REFERENCE #number #authors Pouwels P.H., Enger-Valk B.E., Brammar W.J. #book Cloning Vectors, Elsvier 1985 and supplements #comment vector I-A-iv-20 COMMENT This Sequence was obtained 3-MAR-1986 from J. Messing, Waksman Institute, NJ on floppy disk. Revised 16-DEC-1986 by F. Pfeiffer: 1062/3 'AT' to 'TA' to match revised sequence of PBR322 The beta-galactosidase mRNA sequence including the multiple cloning site of M13mp19 is on the strand complementary to that shown. KEYWORDS CROSSREFERENCE #complement VecBase(3):pUC19 #prerevised GenBank(50):M11662, EMBL(11):ARPuc19 #parent VecBase(3):pUC13, VecBase(3):M13mp19, VecSource(3):bGal19 PARENT Features of pUC19c (2686 bp) residue source 1- 137 2074-2210 pBR322 138- 237 2252-2351 pBR322 238- 395 1461-1304 (c) Lac-Operon 396- 452 57- 1 (c) polylinker of M13mp19 455- 682 1298-1071 (c) Lac-Operon 683-2686 2352-4355 pBR322 Conflict (cfl) and Mutations (mut): pUC19c source mut 1308 A G 2977 pBR322 linked to increased copy number mut 1942 A G 3611 pBR322 mut 2243 T C 3912 pBR322 FEATURE 1629-2417 789-1 (c) Ap-R; b-lactamase POLYLINKER HindIII-SphI-PstI-SalI-XbaI-BamHI-SmaI-KpnI-SacI-EcoRI SELECTION #resistance Ap #indicator beta-galactosidase SUMMARY pUC19c #length 2686 #checksum 4465. COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers source join(863..2686,1..830) /organism="Cloning vector pUC19c" /mol_type="genomic DNA" /db_xref="taxon:174689" /label=source:Cloning vector pUC19c /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind complement(427..449) /label=pGEX 3 primer /ApEinfo_fwdcolor="#ff8000" /ApEinfo_revcolor="#800080" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind 429..446 /label=pGEX3_01R /ApEinfo_fwdcolor="#ff8000" /ApEinfo_revcolor="#800080" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind 162..179 /label=Amp_01R /ApEinfo_fwdcolor="#ff8000" /ApEinfo_revcolor="#800080" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature complement(706..830) /label=LacZ alpha tln start- MCS for blue/white screen /ApEinfo_fwdcolor="#8080ff" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind 776..793 /label=M13-fwd /ApEinfo_fwdcolor="#ff8000" /ApEinfo_revcolor="#800080" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind 759..776 /label=pUC19_Univ_01F /ApEinfo_fwdcolor="#ff8000" /ApEinfo_revcolor="#800080" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" CDS complement(637..705) /label=LacZ alpha /ApEinfo_fwdcolor="#6495ed" /ApEinfo_revcolor="#6495ed" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind complement(807..834) /label=pUC19_MlyI_01F /ApEinfo_fwdcolor="#ff8000" /ApEinfo_revcolor="#800080" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind complement(863..879) /label=M13-rev (DNA Core) /ApEinfo_fwdcolor="#ff8000" /ApEinfo_revcolor="#800080" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" rep_origin complement(1247..1929) /label=ColE1 origin /ApEinfo_fwdcolor="gray50" /ApEinfo_revcolor="gray50" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_binding complement(885..907) /label=LacO /ApEinfo_fwdcolor="#6495ed" /ApEinfo_revcolor="#6495ed" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" CDS complement(2027..2686) /label=AmpR /ApEinfo_fwdcolor="yellow" /ApEinfo_revcolor="yellow" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind complement(893..910) /label=LacO_01F /ApEinfo_fwdcolor="#ff8000" /ApEinfo_revcolor="#800080" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind 863..889 /label=pUC19_ZraI_01R /ApEinfo_fwdcolor="#ff8000" /ApEinfo_revcolor="#800080" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" exon 835..862 /label=T790_28bp /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" variation 839..839 /label=T790M, 2369C>T /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#ff00ff" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" ORIGIN 1 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 61 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 121 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 181 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 241 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 301 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt 361 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg 421 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc 481 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct 541 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc 601 gcacagatgc gtaaggagaa aataccgcat caggcgCCAT TCGCCATTCA GGCTGCGCAA 661 CTGTTGGGAA GGGCGATCGG TGCGGGCCTC TTCGCTATTA CGCCAGCTGG CGAAAGGGGG 721 ATGTGCTGCA AGGCGATTAA GTTGGGTAAC GCCAGGGTTT TCCCAGTCAC GACGTTGTAA 781 AACGACGGCC AGTGAATTCG AGCTCGGTAC CCGGGGATCC TCTAGAGTCG TgatATCATG 841 CAGCTCATGC CCTTCGtagg acgtcatagc tgtttcctgt gtgaaattgt tatccgctca 901 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag 961 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt 1021 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc 1081 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 1141 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 1201 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 1261 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 1321 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 1381 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 1441 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 1501 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 1561 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 1621 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 1681 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag 1741 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 1801 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 1861 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 1921 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 1981 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 2041 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 2101 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 2161 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 2221 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 2281 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta 2341 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 2401 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 2461 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 2521 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 2581 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 2641 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcat //