LOCUS       New_DNA                 2686 bp ds-DNA     circular     28-APR-2017
DEFINITION  Cloning vector pUC19c, complete sequence.
ACCESSION   L09137 X02514
VERSION     L09137.2  GI:20141090
KEYWORDS    .
SOURCE      Cloning vector pUC19c
  ORGANISM  Cloning vector pUC19c other sequences; artificial sequences;
            vectors.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Yanisch-Perron,C., Vieira,J. and Messing,J.
  TITLE     Improved M13 phage cloning vectors and host strains: nucleotide
            sequences of the M13mp18 and pUC19 vectors
  JOURNAL   Gene 33 (1), 103-119 (1985)
   PUBMED   2985470
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Chambers,S.P., Prior,S.E., Barstow,D.A. and Minton,N.P.
  TITLE     The pMTL nic- cloning vectors. I. Improved pUC polylinker regions
            to facilitate the use of sonicated DNA for nucleotide sequencing
  JOURNAL   Gene 68 (1), 139-149 (1988)
   PUBMED   2851488
REFERENCE   3  (bases 1 to 2686)
  AUTHORS   Gilbert,W.
  TITLE     Obtained from VecBase 3.0
  JOURNAL   Unpublished
REFERENCE   4  (bases 1 to 2686)
  AUTHORS   Messing,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-1993) Department of Biochemistry, University of
            Minnesota, St. Paul, MN 55108, USA
REFERENCE   5  (bases 1 to 2686)
  AUTHORS   Messing,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-APR-2002) Rutgers, The State University of New
            Jersey, Waksman Institute of Microbiology, 190 Frelinghuysen Road,
            Piscataway, NJ 08854-8020, USA
  REMARK    Sequence update by submitter
COMMENT     
COMMENT     On Apr 11, 2002 this sequence version replaced gi:209213. These
            data and their annotation were supplied to GenBank by Will Gilbert
            under the auspices of the GenBank Currator Program. pUC19c -
            Cloning vector (beta-galactosidase mRNA on complementary strand)
            ENTRY PUC19C                    #TYPE DNA CIRCULAR TITLE pUC19c -
            Cloning vector               (beta-galactosidase mRNA on
            complementary strand) DATE      03-FEB-1986    #sequence
            16-DEC-1986 ACCESSION VB0033 SOURCE    artificial COLLECTION  ATCC
            37254 REFERENCE    #number    #authors  Norrander J., Kempe T.,
            Messing J.    #journal Gene (1983) 26: 101-106 REFERENCE    #number
            1    #authors  Yanisch-Perron C., Vieira J., Messing J.    #journal
            Gene (1985) 33: 103-119    #comment shows the complete compiled
            sequence REFERENCE    #number  2    #authors  Chambers,S.P., et al.
            #journal Gene (1988) 68: 139-149    #describes mutation at nt1308
            and its effect on copy number REFERENCE    #number    #authors
            Pouwels P.H., Enger-Valk B.E., Brammar W.J.    #book    Cloning
            Vectors, Elsvier 1985 and supplements    #comment  vector I-A-iv-20
            COMMENT    This Sequence was obtained 3-MAR-1986 from J. Messing,
            Waksman    Institute, NJ on floppy disk.    Revised 16-DEC-1986 by
            F. Pfeiffer:    1062/3 'AT' to 'TA' to match revised sequence of
            PBR322    The beta-galactosidase mRNA sequence including the
            multiple    cloning site of M13mp19 is on the strand complementary
            to that    shown. KEYWORDS CROSSREFERENCE    #complement
            VecBase(3):pUC19    #prerevised      GenBank(50):M11662,
            EMBL(11):ARPuc19    #parent      VecBase(3):pUC13,
            VecBase(3):M13mp19, VecSource(3):bGal19 PARENT    Features of
            pUC19c (2686 bp)      residue     source       1- 137  2074-2210
            pBR322     138- 237  2252-2351 pBR322     238- 395  1461-1304 (c)
            Lac-Operon     396- 452    57-   1 (c) polylinker of M13mp19 455-
            682  1298-1071 (c) Lac-Operon     683-2686  2352-4355 pBR322
            Conflict (cfl) and Mutations (mut):         pUC19c  source    mut
            1308  A  G  2977   pBR322   linked to increased copy number    mut
            1942  A  G  3611   pBR322    mut 2243  T  C  3912   pBR322 FEATURE
            1629-2417 789-1 (c) Ap-R; b-lactamase POLYLINKER
            HindIII-SphI-PstI-SalI-XbaI-BamHI-SmaI-KpnI-SacI-EcoRI SELECTION
            #resistance  Ap    #indicator beta-galactosidase SUMMARY  pUC19c
            #length 2686   #checksum 4465.
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     source          join(863..2686,1..830)
                     /organism="Cloning vector pUC19c"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:174689"
                     /label=source:Cloning vector pUC19c
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(427..449)
                     /label=pGEX 3 primer
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#800080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     429..446
                     /label=pGEX3_01R
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#800080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     162..179
                     /label=Amp_01R
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#800080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(706..830)
                     /label=LacZ alpha tln start- MCS for blue/white screen
                     /ApEinfo_fwdcolor="#8080ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     776..793
                     /label=M13-fwd
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#800080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     759..776
                     /label=pUC19_Univ_01F
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#800080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(637..705)
                     /label=LacZ alpha
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(807..834)
                     /label=pUC19_MlyI_01F
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#800080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(863..879)
                     /label=M13-rev (DNA Core)
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#800080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(1247..1929)
                     /label=ColE1 origin
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    complement(885..907)
                     /label=LacO
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(2027..2686)
                     /label=AmpR
                     /ApEinfo_fwdcolor="yellow"
                     /ApEinfo_revcolor="yellow"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(893..910)
                     /label=LacO_01F
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#800080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     863..889
                     /label=pUC19_ZraI_01R
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#800080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     exon            835..862
                     /label=T790_28bp
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     variation       839..839
                     /label=T790M, 2369C>T
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
       61 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
      121 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
      181 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
      241 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
      301 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
      361 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg
      421 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
      481 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct
      541 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
      601 gcacagatgc gtaaggagaa aataccgcat caggcgCCAT TCGCCATTCA GGCTGCGCAA
      661 CTGTTGGGAA GGGCGATCGG TGCGGGCCTC TTCGCTATTA CGCCAGCTGG CGAAAGGGGG
      721 ATGTGCTGCA AGGCGATTAA GTTGGGTAAC GCCAGGGTTT TCCCAGTCAC GACGTTGTAA
      781 AACGACGGCC AGTGAATTCG AGCTCGGTAC CCGGGGATCC TCTAGAGTCG TgatATCATG
      841 CAGCTCATGC CCTTCGtagg acgtcatagc tgtttcctgt gtgaaattgt tatccgctca
      901 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
      961 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     1021 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     1081 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     1141 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     1201 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     1261 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     1321 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     1381 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     1441 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     1501 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     1561 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     1621 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     1681 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     1741 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     1801 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     1861 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     1921 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     1981 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     2041 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     2101 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     2161 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     2221 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     2281 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     2341 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     2401 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     2461 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     2521 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     2581 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     2641 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcat 
//
