LOCUS       Exported               12425 bp ds-DNA     circular SYN 23-JUL-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12425)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Jul 24, 2018 from SnapGene Viewer 4.2.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12425
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     misc_feature    874..936
                     /label=3' TR
     misc_feature    1113..1344
                     /label=Core Insulator
     misc_feature    1346..1366
                     /label=H1
     misc_feature    1367..1977
                     /label=SH LHA
     misc_feature    1886..1888
                     /label=PAM
     misc_feature    1889..1907
                     /label=SH gRNA 4
     misc_feature    1960..1968
                     /label=SHS231
     misc_feature    1978..2030
                     /label=Bxb1 attP
     promoter        2058..2269
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             2298..2321
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2328..2348
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2373..6473
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     misc_feature    6106..7090
                     /label=11-16-15 100% Seq MP7
     misc_feature    6108..7041
                     /label=12-16-15 100% Seq MP13 Midi
     CDS             6474..6521
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6528..6581
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6582..6980
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    6996..7584
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(7467..7478)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7656..7889
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7967..8088
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      8128..8262
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     misc_feature    8269..8310
                     /label=fC31 attP
     misc_feature    8311..8838
                     /label=SH RHA
     misc_feature    8489..8491
                     /label=PAM
     misc_feature    8492..8510
                     /label=SH gRNA 5
     misc_feature    8839..8859
                     /label=H2
     polyA_signal    9000..9117
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    complement(9374..9604)
                     /label=Core Insulator
     misc_feature    9879..9913
                     /label=5' TR
     primer_bind     complement(10404..10420)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    10428..10444
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(10452..10482)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10497..10518
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(10806..11394)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(11565..12425)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgc gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaaa caaaaactca
     1081 aaatttcttc tataaagtaa caaaactttt atgagggaca gccccccccc aaagccccca
     1141 gggatgtaat tacgtccctc ccccgctagg gggcagcagc gagccgcccg gggctccgct
     1201 ccggtccggc gctccccccg catccccgag ccggcagcgt gcggggacag cccgggcacg
     1261 gggaaggtgg cacgggatcg ctttcctctg aacgcttctc gctgctcttt gagcctgcag
     1321 acacctgggg ggatacgggg aaaaggcctc caaggccaga ctagaggtac ccattgagtt
     1381 ctgaggtggt ttgtcataca gcaatggcaa tcagatatat ccacacaaat ataattttag
     1441 tttatatttc tgctactgca gttctcatct tattctgagg atatgtgaca aaataattct
     1501 ttcaaaaata ttgatgctgt gctagattac tattttgaat gaattactag acaaatactt
     1561 catatgtatc tttttttttt tttttttttt tttttttttt tttttttgag acggagtctc
     1621 gctctgtcgc ccaggctgga gtgcagtggc gggatctcgg ctcactgcaa tatgtatctt
     1681 attatgtggg tttacacatt atttatctta ttgatttaac ttcaaaacta aactttagtt
     1741 tagctcttgg gccctatctg gaaagggtca tcttttaatc accattaaat cactgaagtc
     1801 atcagtttat tcaaagtact ctgcacaaat tagcattctt tagtggttgt gaaataaata
     1861 gactttaaac ttatcattaa tattcccaat ggtactatgg gggaggcaaa attttctgtc
     1921 ttcttagtgg tttttttttt ttggctaggg ctaaggatta aactgtcaag cttcgaggtg
     1981 gtttgtctgg tcaaccaccg cggtctcagt ggtgtacggt acaaaccccg agtcgagtgg
     2041 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
     2101 aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
     2161 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
     2221 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg tgtcgtgacg
     2281 cgggatccgc caccatggat tacaaagacg atgacgataa gatggcccca aagaagaagc
     2341 ggaaggtcgg tatccacgga gtcccagcag ccgacaagaa gtacagcatc ggcctggaca
     2401 tcggcaccaa ctctgtgggc tgggccgtga tcaccgacga gtacaaggtg cccagcaaga
     2461 aattcaaggt gctgggcaac accgaccggc acagcatcaa gaagaacctg atcggagccc
     2521 tgctgttcga cagcggcgaa acagccgagg ccacccggct gaagagaacc gccagaagaa
     2581 gatacaccag acggaagaac cggatctgct atctgcaaga gatcttcagc aacgagatgg
     2641 ccaaggtgga cgacagcttc ttccacagac tggaagagtc cttcctggtg gaagaggata
     2701 agaagcacga gcggcacccc atcttcggca acatcgtgga cgaggtggcc taccacgaga
     2761 agtaccccac catctaccac ctgagaaaga aactggtgga cagcaccgac aaggccgacc
     2821 tgcggctgat ctatctggcc ctggcccaca tgatcaagtt ccggggccac ttcctgatcg
     2881 agggcgacct gaaccccgac aacagcgacg tggacaagct gttcatccag ctggtgcaga
     2941 cctacaacca gctgttcgag gaaaacccca tcaacgccag cggcgtggac gccaaggcca
     3001 tcctgtctgc cagactgagc aagagcagac ggctggaaaa tctgatcgcc cagctgcccg
     3061 gcgagaagaa gaatggcctg ttcggcaacc tgattgccct gagcctgggc ctgaccccca
     3121 acttcaagag caacttcgac ctggccgagg atgccaaact gcagctgagc aaggacacct
     3181 acgacgacga cctggacaac ctgctggccc agatcggcga ccagtacgcc gacctgtttc
     3241 tggccgccaa gaacctgtcc gacgccatcc tgctgagcga catcctgaga gtgaacaccg
     3301 agatcaccaa ggcccccctg agcgcctcta tgatcaagag atacgacgag caccaccagg
     3361 acctgaccct gctgaaagct ctcgtgcggc agcagctgcc tgagaagtac aaagagattt
     3421 tcttcgacca gagcaagaac ggctacgccg gctacattga cggcggagcc agccaggaag
     3481 agttctacaa gttcatcaag cccatcctgg aaaagatgga cggcaccgag gaactgctcg
     3541 tgaagctgaa cagagaggac ctgctgcgga agcagcggac cttcgacaac ggcagcatcc
     3601 cccaccagat ccacctggga gagctgcacg ccattctgcg gcggcaggaa gatttttacc
     3661 cattcctgaa ggacaaccgg gaaaagatcg agaagatcct gaccttccgc atcccctact
     3721 acgtgggccc tctggccagg ggaaacagca gattcgcctg gatgaccaga aagagcgagg
     3781 aaaccatcac cccctggaac ttcgaggaag tggtggacaa gggcgcttcc gcccagagct
     3841 tcatcgagcg gatgaccaac ttcgataaga acctgcccaa cgagaaggtg ctgcccaagc
     3901 acagcctgct gtacgagtac ttcaccgtgt ataacgagct gaccaaagtg aaatacgtga
     3961 ccgagggaat gagaaagccc gccttcctga gcggcgagca gaaaaaggcc atcgtggacc
     4021 tgctgttcaa gaccaaccgg aaagtgaccg tgaagcagct gaaagaggac tacttcaaga
     4081 aaatcgagtg cttcgactcc gtggaaatct ccggcgtgga agatcggttc aacgcctccc
     4141 tgggcacata ccacgatctg ctgaaaatta tcaaggacaa ggacttcctg gacaatgagg
     4201 aaaacgagga cattctggaa gatatcgtgc tgaccctgac actgtttgag gacagagaga
     4261 tgatcgagga acggctgaaa acctatgccc acctgttcga cgacaaagtg atgaagcagc
     4321 tgaagcggcg gagatacacc ggctggggca ggctgagccg gaagctgatc aacggcatcc
     4381 gggacaagca gtccggcaag acaatcctgg atttcctgaa gtccgacggc ttcgccaaca
     4441 gaaacttcat gcagctgatc cacgacgaca gcctgacctt taaagaggac atccagaaag
     4501 cccaggtgtc cggccagggc gatagcctgc acgagcacat tgccaatctg gccggcagcc
     4561 ccgccattaa gaagggcatc ctgcagacag tgaaggtggt ggacgagctc gtgaaagtga
     4621 tgggccggca caagcccgag aacatcgtga tcgaaatggc cagagagaac cagaccaccc
     4681 agaagggaca gaagaacagc cgcgagagaa tgaagcggat cgaagagggc atcaaagagc
     4741 tgggcagcca gatcctgaaa gaacaccccg tggaaaacac ccagctgcag aacgagaagc
     4801 tgtacctgta ctacctgcag aatgggcggg atatgtacgt ggaccaggaa ctggacatca
     4861 accggctgtc cgactacgat gtggaccata tcgtgcctca gagctttctg aaggacgact
     4921 ccatcgacaa caaggtgctg accagaagcg acaagaaccg gggcaagagc gacaacgtgc
     4981 cctccgaaga ggtcgtgaag aagatgaaga actactggcg gcagctgctg aacgccaagc
     5041 tgattaccca gagaaagttc gacaatctga ccaaggccga gagaggcggc ctgagcgaac
     5101 tggataaggc cggcttcatc aagagacagc tggtggaaac ccggcagatc acaaagcacg
     5161 tggcacagat cctggactcc cggatgaaca ctaagtacga cgagaatgac aagctgatcc
     5221 gggaagtgaa agtgatcacc ctgaagtcca agctggtgtc cgatttccgg aaggatttcc
     5281 agttttacaa agtgcgcgag atcaacaact accaccacgc ccacgacgcc tacctgaacg
     5341 ccgtcgtggg aaccgccctg atcaaaaagt accctaagct ggaaagcgag ttcgtgtacg
     5401 gcgactacaa ggtgtacgac gtgcggaaga tgatcgccaa gagcgagcag gaaatcggca
     5461 aggctaccgc caagtacttc ttctacagca acatcatgaa ctttttcaag accgagatta
     5521 ccctggccaa cggcgagatc cggaagcggc ctctgatcga gacaaacggc gaaaccgggg
     5581 agatcgtgtg ggataagggc cgggattttg ccaccgtgcg gaaagtgctg agcatgcccc
     5641 aagtgaatat cgtgaaaaag accgaggtgc agacaggcgg cttcagcaaa gagtctatcc
     5701 tgcccaagag gaacagcgat aagctgatcg ccagaaagaa ggactgggac cctaagaagt
     5761 acggcggctt cgacagcccc accgtggcct attctgtgct ggtggtggcc aaagtggaaa
     5821 agggcaagtc caagaaactg aagagtgtga aagagctgct ggggatcacc atcatggaaa
     5881 gaagcagctt cgagaagaat cccatcgact ttctggaagc caagggctac aaagaagtga
     5941 aaaaggacct gatcatcaag ctgcctaagt actccctgtt cgagctggaa aacggccgga
     6001 agagaatgct ggcctctgcc ggcgaactgc agaagggaaa cgaactggcc ctgccctcca
     6061 aatatgtgaa cttcctgtac ctggccagcc actatgagaa gctgaagggc tcccccgagg
     6121 ataatgagca gaaacagctg tttgtggaac agcacaagca ctacctggac gagatcatcg
     6181 agcagatcag cgagttctcc aagagagtga tcctggccga cgctaatctg gacaaagtgc
     6241 tgtccgccta caacaagcac cgggataagc ccatcagaga gcaggccgag aatatcatcc
     6301 acctgtttac cctgaccaat ctgggagccc ctgccgcctt caagtacttt gacaccacca
     6361 tcgaccggaa gaggtacacc agcaccaaag aggtgctgga cgccaccctg atccaccaga
     6421 gcatcaccgg cctgtacgag acacggatcg acctgtctca gctgggaggc gacaagcgtc
     6481 ctgctgctac taagaaagct ggtcaagcta agaaaaagaa agctagcgag ggcagaggaa
     6541 gtctgctaac atgcggtgac gtcgaggaga atcctggccc aatggccaag cctttgtctc
     6601 aagaagaatc caccctcatt gaaagagcaa cggctacaat caacagcatc cccatctctg
     6661 aagactacag cgtcgccagc gcagctctct ctagcgacgg ccgcatcttc actggtgtca
     6721 atgtatatca ttttactggg ggaccttgtg cagaactcgt ggtgctgggc actgctgctg
     6781 ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg aaatgagaac aggggcatct
     6841 tgagcccctg cggacggtgc cgacaggtgc ttctcgatct gcatcctggg atcaaagcca
     6901 tagtgaagga cagtgatgga cagccgacgg cagttgggat tcgtgaattg ctgccctctg
     6961 gttatgtgtg ggagggctaa acgcgttaag tcgacaatca acctctggat tacaaaattt
     7021 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     7081 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     7141 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     7201 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     7261 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     7321 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     7381 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     7441 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     7501 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     7561 tctccctttg ggccgcctcc ccgcgtcgac tttaagacca atgacttaca aggcagctgt
     7621 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     7681 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     7741 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     7801 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     7861 cttttagtca gtgtggaaaa tctctagcag tacgtatagt agttcatgtc atcttattat
     7921 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc
     7981 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     8041 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct
     8101 ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     8161 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     8221 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagaagt gccccaactg
     8281 gggtaacctt tgagttctct cagttggggg actagtgcgt ggacaaagag cagtaaattg
     8341 tgaaaatgga acaaggcaag ggggcttaga ctacagtagt taatcatcaa gaagtgacaa
     8401 aaaaaataat ggttagttaa taagatttgt ttaagcagat ttctcccagc tttagctctc
     8461 tgtctctggt gatcagaatg cactccttcc ttcagactca gtgagcacat cttccacatg
     8521 gaagatttct tctctagctt ttaggaaatc cagagaaccc tttttgtatc tgctgttttt
     8581 ttttttttaa atgtctttaa ctcaaaacaa tttatgtgcc aggatgacat atctttggat
     8641 aatgtgttct gaactccttc agtacatacg tatataaatt aaagcaaata ttttttatga
     8701 taagctggca taatagtttc ataatttaat cactgattta aaaatttaat taaaattatt
     8761 ttttgatatt ttgtgtaata atttttgagg agtatctttt gtgcttaatg agtggcagat
     8821 gacacccatg ttcttagcta cgcgttaact aagtaagcgg ccgccccggt atcgacaatc
     8881 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     8941 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgcgttaact aactaaactt
     9001 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     9061 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     9121 tgtctggaat tgactcaaat gatgtcaatt agtctatcag aagctatctg gtctcccttc
     9181 cgggggacaa gacatccctg tttaatattt aaacagcagt gttcccaaac tgggttctta
     9241 tatcccttgc tctggtcaac caggttgcag ggtttcctgt cctcacagga acgaagtccc
     9301 taaagaaaca gtggcagcca ggtttagccc cggaattgac tggattcctt ttttagggcc
     9361 cattggtatg gctttttccc cgtatccccc caggtgtctg caggctcaaa gagcagcgag
     9421 aagcgttcag aggaaagcga tcccgtgcca ccttccccgt gcccgggctg tccccgcacg
     9481 ctgccggctc ggggatgcgg ggggagcgcc ggaccggagc ggagccccgg gcggctcgct
     9541 gctgccccct agcgggggag ggacgtaatt acatccctgg gggctttggg ggggggctgt
     9601 ccctgatatc tataacaaga aaatatatat ataataagtt atcacgtaag tagaacatga
     9661 aataacaata taattatcgt atgagttaaa tcttaaaagt cacgtaaaag ataatcatgc
     9721 gtcattttga ctcacgcggt cgttatagtt caaaatcagt gacacttacc gcattgacaa
     9781 gcacgcctca cgggagctcc aagcggcgac tgagatgtcc taaatgcaca gcgacggatt
     9841 cgcgctattt agaaagagag agcaatattt caagaatgca tgcgtcaatt ttacgcagac
     9901 tatctttcta gggttaatct agctgcatca ggatcatatc gtcgggtctt ttttccggct
     9961 cagtcatcgc ccaagctggc gctatctggg catcggggag gaagaagccc gtgccttttc
    10021 ccgcgaggtt gaagcggcat ggaaagagtt tgccgaggat gactgctgct gcattgacgt
    10081 tgagcgaaaa cgcacgttta ccatgatgat tcgggaaggt gtggccatgc acgcctttaa
    10141 cggtgaactg ttcgttcagg ccacctggga taccagttcg tcgcggcttt tccggacaca
    10201 gttccggatg gtcagcccga agcgcatcag caacccgaac aataccggcg acagccggaa
    10261 ctgccgtgcc ggtgtgcaga ttaatgacag cggtgcggcg ctgggatatt acgtcagcga
    10321 ggacgggtat cctggctgga tgccgcagaa atggacatgg ataccccgtg agttacccgg
    10381 cgggcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
    10441 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
    10501 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
    10561 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
    10621 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
    10681 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
    10741 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
    10801 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
    10861 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
    10921 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
    10981 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
    11041 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
    11101 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
    11161 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
    11221 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
    11281 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
    11341 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
    11401 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
    11461 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
    11521 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
    11581 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
    11641 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
    11701 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
    11761 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
    11821 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
    11881 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
    11941 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
    12001 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
    12061 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
    12121 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
    12181 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
    12241 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
    12301 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
    12361 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
    12421 ctcat
//
