LOCUS Exported 9605 bp ds-DNA circular SYN 23-JUL-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9605) AUTHORS Trial User TITLE Direct Submission JOURNAL Exported Jul 24, 2018 from SnapGene Viewer 4.2.1 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9605 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 1..845 /label=11-30-14 99% Seq MP6 promoter 50..290 /label=U6 promoter /note="RNA polymerase III promoter for human U6 snRNA" misc_feature 299..317 /label=SHCtrl gRNA4 misc_RNA 318..393 /label=gRNA scaffold /note="guide RNA scaffold for the CRISPR/Cas9 system" misc_feature 400..425 /label=11-30-14 99% Seq MP6 promoter 426..666 /label=U6 promoter /note="RNA polymerase III promoter for human U6 snRNA" misc_feature 675..694 /label=SHCtrl gRNA5 misc_RNA 695..770 /label=gRNA scaffold /note="guide RNA scaffold for the CRISPR/Cas9 system" promoter 1086..1363 /label=chicken beta-actin promoter CDS 1613..1678 /codon_start=1 /product="three tandem FLAG(R) epitope tags, followed by an enterokinase cleavage site" /label=3xFLAG /translation="DYKDHDGDYKDHDIDYKDDDDK" CDS 1685..1705 /codon_start=1 /product="nuclear localization signal of SV40 large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 1730..5830 /codon_start=1 /product="Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=Cas9 /note="generates RNA-guided double strand breaks in DNA" /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD ATLIHQSITGLYETRIDLSQLGGD" CDS 5831..5878 /codon_start=1 /product="bipartite nuclear localization signal from nucleoplasmin" /label=nucleoplasmin NLS /translation="KRPAATKKAGQAKKKK" CDS 5885..5938 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" CDS 5945..6664 /codon_start=1 /product="Emerald GFP" /label=EmGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" polyA_signal 6698..6905 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" repeat_region 6914..7054 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" rep_origin 7129..7584 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 7866..7970 /gene="bla" /label=AmpR promoter CDS 7971..8831 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 9002..9590 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" ORIGIN 1 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttgtg agggcctatt 61 tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga taattggaat 121 taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt 181 tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata tgcttaccgt 241 aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccgc 301 ctcccccata gtaccatgtt ttagagctag aaatagcaag ttaaaataag gctagtccgt 361 tatcaacttg aaaaagtggc accgagtcgg tgcttttttg accaacgagc aagataagga 421 ctagggaggg cctatttccc atgattcctt catatttgca tatacgatac aaggctgtta 481 gagagataat tggaattaat ttgactgtaa acacaaagat attagtacaa aatacgtgac 541 gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt aaaatggact 601 atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga 661 aaggacgaaa caccgatgtg ctcactgagt ctgagtttta gagctagaaa tagcaagtta 721 aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc ttttttctcg 781 agaaactagt aaggtacccg ttacataact tacggtaaat ggcccgcctg gctgaccgcc 841 caacgacccc cgcccattga cgtcaatagt aacgccaata gggactttcc attgacgtca 901 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 961 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt gtgcccagta 1021 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 1081 catggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc 1141 cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg 1201 gggggggcgc gcgccaggcg gggcggggcg gggcgagggg cggggcgggg cgaggcggag 1261 aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg 1321 gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgacgct 1381 gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg ctctgactga 1441 ccgcgttact cccacaggtg agcgggcggg acggcccttc tcctccgggc tgtaattagc 1501 tgagcaagag gtaagggttt aagggatggt tggttggtgg ggtattaatg tttaattacc 1561 tggagcacct gcctgaaatc actttttttc aggttggacc ggtgccacca tggactataa 1621 ggaccacgac ggagactaca aggatcatga tattgattac aaagacgatg acgataagat 1681 ggccccaaag aagaagcgga aggtcggtat ccacggagtc ccagcagccg acaagaagta 1741 cagcatcggc ctggacatcg gcaccaactc tgtgggctgg gccgtgatca ccgacgagta 1801 caaggtgccc agcaagaaat tcaaggtgct gggcaacacc gaccggcaca gcatcaagaa 1861 gaacctgatc ggagccctgc tgttcgacag cggcgaaaca gccgaggcca cccggctgaa 1921 gagaaccgcc agaagaagat acaccagacg gaagaaccgg atctgctatc tgcaagagat 1981 cttcagcaac gagatggcca aggtggacga cagcttcttc cacagactgg aagagtcctt 2041 cctggtggaa gaggataaga agcacgagcg gcaccccatc ttcggcaaca tcgtggacga 2101 ggtggcctac cacgagaagt accccaccat ctaccacctg agaaagaaac tggtggacag 2161 caccgacaag gccgacctgc ggctgatcta tctggccctg gcccacatga tcaagttccg 2221 gggccacttc ctgatcgagg gcgacctgaa ccccgacaac agcgacgtgg acaagctgtt 2281 catccagctg gtgcagacct acaaccagct gttcgaggaa aaccccatca acgccagcgg 2341 cgtggacgcc aaggccatcc tgtctgccag actgagcaag agcagacggc tggaaaatct 2401 gatcgcccag ctgcccggcg agaagaagaa tggcctgttc ggaaacctga ttgccctgag 2461 cctgggcctg acccccaact tcaagagcaa cttcgacctg gccgaggatg ccaaactgca 2521 gctgagcaag gacacctacg acgacgacct ggacaacctg ctggcccaga tcggcgacca 2581 gtacgccgac ctgtttctgg ccgccaagaa cctgtccgac gccatcctgc tgagcgacat 2641 cctgagagtg aacaccgaga tcaccaaggc ccccctgagc gcctctatga tcaagagata 2701 cgacgagcac caccaggacc tgaccctgct gaaagctctc gtgcggcagc agctgcctga 2761 gaagtacaaa gagattttct tcgaccagag caagaacggc tacgccggct acattgacgg 2821 cggagccagc caggaagagt tctacaagtt catcaagccc atcctggaaa agatggacgg 2881 caccgaggaa ctgctcgtga agctgaacag agaggacctg ctgcggaagc agcggacctt 2941 cgacaacggc agcatccccc accagatcca cctgggagag ctgcacgcca ttctgcggcg 3001 gcaggaagat ttttacccat tcctgaagga caaccgggaa aagatcgaga agatcctgac 3061 cttccgcatc ccctactacg tgggccctct ggccagggga aacagcagat tcgcctggat 3121 gaccagaaag agcgaggaaa ccatcacccc ctggaacttc gaggaagtgg tggacaaggg 3181 cgcttccgcc cagagcttca tcgagcggat gaccaacttc gataagaacc tgcccaacga 3241 gaaggtgctg cccaagcaca gcctgctgta cgagtacttc accgtgtata acgagctgac 3301 caaagtgaaa tacgtgaccg agggaatgag aaagcccgcc ttcctgagcg gcgagcagaa 3361 aaaggccatc gtggacctgc tgttcaagac caaccggaaa gtgaccgtga agcagctgaa 3421 agaggactac ttcaagaaaa tcgagtgctt cgactccgtg gaaatctccg gcgtggaaga 3481 tcggttcaac gcctccctgg gcacatacca cgatctgctg aaaattatca aggacaagga 3541 cttcctggac aatgaggaaa acgaggacat tctggaagat atcgtgctga ccctgacact 3601 gtttgaggac agagagatga tcgaggaacg gctgaaaacc tatgcccacc tgttcgacga 3661 caaagtgatg aagcagctga agcggcggag atacaccggc tggggcaggc tgagccggaa 3721 gctgatcaac ggcatccggg acaagcagtc cggcaagaca atcctggatt tcctgaagtc 3781 cgacggcttc gccaacagaa acttcatgca gctgatccac gacgacagcc tgacctttaa 3841 agaggacatc cagaaagccc aggtgtccgg ccagggcgat agcctgcacg agcacattgc 3901 caatctggcc ggcagccccg ccattaagaa gggcatcctg cagacagtga aggtggtgga 3961 cgagctcgtg aaagtgatgg gccggcacaa gcccgagaac atcgtgatcg aaatggccag 4021 agagaaccag accacccaga agggacagaa gaacagccgc gagagaatga agcggatcga 4081 agagggcatc aaagagctgg gcagccagat cctgaaagaa caccccgtgg aaaacaccca 4141 gctgcagaac gagaagctgt acctgtacta cctgcagaat gggcgggata tgtacgtgga 4201 ccaggaactg gacatcaacc ggctgtccga ctacgatgtg gaccatatcg tgcctcagag 4261 ctttctgaag gacgactcca tcgacaacaa ggtgctgacc agaagcgaca agaaccgggg 4321 caagagcgac aacgtgccct ccgaagaggt cgtgaagaag atgaagaact actggcggca 4381 gctgctgaac gccaagctga ttacccagag aaagttcgac aatctgacca aggccgagag 4441 aggcggcctg agcgaactgg ataaggccgg cttcatcaag agacagctgg tggaaacccg 4501 gcagatcaca aagcacgtgg cacagatcct ggactcccgg atgaacacta agtacgacga 4561 gaatgacaag ctgatccggg aagtgaaagt gatcaccctg aagtccaagc tggtgtccga 4621 tttccggaag gatttccagt tttacaaagt gcgcgagatc aacaactacc accacgccca 4681 cgacgcctac ctgaacgccg tcgtgggaac cgccctgatc aaaaagtacc ctaagctgga 4741 aagcgagttc gtgtacggcg actacaaggt gtacgacgtg cggaagatga tcgccaagag 4801 cgagcaggaa atcggcaagg ctaccgccaa gtacttcttc tacagcaaca tcatgaactt 4861 tttcaagacc gagattaccc tggccaacgg cgagatccgg aagcggcctc tgatcgagac 4921 aaacggcgaa accggggaga tcgtgtggga taagggccgg gattttgcca ccgtgcggaa 4981 agtgctgagc atgccccaag tgaatatcgt gaaaaagacc gaggtgcaga caggcggctt 5041 cagcaaagag tctatcctgc ccaagaggaa cagcgataag ctgatcgcca gaaagaagga 5101 ctgggaccct aagaagtacg gcggcttcga cagccccacc gtggcctatt ctgtgctggt 5161 ggtggccaaa gtggaaaagg gcaagtccaa gaaactgaag agtgtgaaag agctgctggg 5221 gatcaccatc atggaaagaa gcagcttcga gaagaatccc atcgactttc tggaagccaa 5281 gggctacaaa gaagtgaaaa aggacctgat catcaagctg cctaagtact ccctgttcga 5341 gctggaaaac ggccggaaga gaatgctggc ctctgccggc gaactgcaga agggaaacga 5401 actggccctg ccctccaaat atgtgaactt cctgtacctg gccagccact atgagaagct 5461 gaagggctcc cccgaggata atgagcagaa acagctgttt gtggaacagc acaagcacta 5521 cctggacgag atcatcgagc agatcagcga gttctccaag agagtgatcc tggccgacgc 5581 taatctggac aaagtgctgt ccgcctacaa caagcaccgg gataagccca tcagagagca 5641 ggccgagaat atcatccacc tgtttaccct gaccaatctg ggagcccctg ccgccttcaa 5701 gtactttgac accaccatcg accggaagag gtacaccagc accaaagagg tgctggacgc 5761 caccctgatc caccagagca tcaccggcct gtacgagaca cggatcgacc tgtctcagct 5821 gggaggcgac aaaaggccgg cggccacgaa aaaggccggc caggcaaaaa agaaaaaggc 5881 tagcgagggc agaggaagtc tgctaacatg cggtgacgtc gaggagaatc ctggcccaac 5941 cggtatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct 6001 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac 6061 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc 6121 caccctcgtg accaccttca cctacggcgt gcagtgcttc gcccgctacc ccgaccacat 6181 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat 6241 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac 6301 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg 6361 gcacaagctg gagtacaact acaacagcca caaggtctat atcaccgccg acaagcagaa 6421 gaacggcatc aaggtgaact tcaagacccg ccacaacatc gaggacggca gcgtgcagct 6481 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa 6541 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat 6601 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa 6661 gtaagaattc taactagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca 6721 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc 6781 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg 6841 gggggtgggg tggggcagga cagcaagggg gaggattggg aagagaatag caggcatgct 6901 ggggagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc 6961 tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc 7021 tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt 7081 acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta 7141 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca 7201 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct 7261 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc 7321 acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat 7381 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc 7441 aaactggaac aacactcaac cctatctcgg gctattcttt tgatttataa gggattttgc 7501 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta 7561 acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg 7621 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc 7681 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga 7741 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt 7801 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa 7861 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca 7921 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc 7981 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc 8041 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt 8101 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt 8161 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg 8221 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact 8281 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg 8341 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga 8401 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg 8461 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa 8521 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac 8581 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc 8641 cggctggctg gtttattgct gataaatctg gagccggtga gcgtggaagc cgcggtatca 8701 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga 8761 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta 8821 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc 8881 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc 8941 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt 9001 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac 9061 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct 9121 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact 9181 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg 9241 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata 9301 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga 9361 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag 9421 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg 9481 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac 9541 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca 9601 acgcg //