LOCUS                   pTL780        4284 bp ds-DNA   circular     25-JUL-2018
DEFINITION  P element transformation vector pGE-attB-GMR, complete sequence.
ACCESSION   FJ791037
VERSION     FJ791037.1  gi:225903563
KEYWORDS    .
SOURCE      P element transformation vector pGE-attB-GMR
  ORGANISM  P element transformation vector pGE-attB-GMR other sequences;
            artificial sequences; vectors.
REFERENCE   1
  AUTHORS   Huang, J., Zhou, W., Dong, W., Watson, A.M., Hong, Y.
  TITLE     From the Cover: Directed, efficient, and versatile modifications of
            the Drosophila genome by genomic engineering
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 106 (20), 8284-8289 (2009)
  PUBMED    19429710
REFERENCE   2
  AUTHORS   Hong, Y., Huang, J.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-FEB-2009) Cell Biology and Physiology, University of
            Pittsburgh, 3500 Terrace Street, S325 BSTWR, Pittsburgh, PA 15261,
            USA
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    720..1397
                     /label=DsRed
                     /ApEinfo_fwdcolor=#ff0a3a
                     /ApEinfo_revcolor=#ff0a3a
     misc_feature    1399..1639
                     /label=SV40 3'UTR
                     /ApEinfo_fwdcolor=#ffb6fc
                     /ApEinfo_revcolor=#ffb6fc
     misc_feature    495..700
                     /label=3xP3 enhancer/hsp70 promoter
                     /ApEinfo_fwdcolor=#00ffff
                     /ApEinfo_revcolor=#00ffff
     CDS             complement(3235..4095)
                     /note="Amp resistance gene"
                     /codon_start=1
                     /transl_table=1
                     /product="beta-lactamase"
                     /protein_id="ACO34946.1"
                     /db_xref="GI:225903565"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /note="Geneious name: beta-lactamase CDS"
                     /label=beta-lactamase
                     /ApEinfo_fwdcolor=#fdff4a
                     /ApEinfo_revcolor=#fdff4a
     misc_feature    489..494
                     /label=BsiWI
                     /ApEinfo_fwdcolor=#c0c1b7
                     /ApEinfo_revcolor=#c0c1b7
     misc_feature    1658..2232
                     /note="5' P-element"
                     /note="Geneious name: 5' P-element"
                     /label=5' P-element
                     /ApEinfo_fwdcolor=#808080
                     /ApEinfo_revcolor=#808080
     misc_feature    join(342..368,371..394)
                     /note="phiC31 attB"
                     /note="Geneious name: phiC31 attB"
                     /label=phiC31 attB
                     /ApEinfo_fwdcolor=#c02dad
                     /ApEinfo_revcolor=#c02dad
     misc_feature    1..235
                     /note="3' P-element"
                     /note="Geneious name: 3' P-element"
                     /label=3' P-element
                     /ApEinfo_fwdcolor=#808080
                     /ApEinfo_revcolor=#808080
     misc_feature    369..369
                     /note="phiC31 attB"
                     /note="Geneious name: phiC31 attB"
                     /label=phiC31 attB(1)
                     /ApEinfo_label=phiC31 attB
                     /ApEinfo_fwdcolor=#c02dad
                     /ApEinfo_revcolor=#c02dad
     misc_feature    369..369
                     /label=attB-P crossover nucleotides
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=cyan
     misc_feature    369..369
                     /label=attP-attB crossover nucleotides
                     /ApEinfo_fwdcolor=#009cff
                     /ApEinfo_revcolor=#009cff
     misc_feature    370..370
                     /note="phiC31 attB"
                     /note="Geneious name: phiC31 attB"
                     /label=phiC31 attB(2)
                     /ApEinfo_label=phiC31 attB
                     /ApEinfo_fwdcolor=#c02dad
                     /ApEinfo_revcolor=#c02dad
     misc_feature    370..370
                     /label=attB-P crossover nucleotides(1)
                     /ApEinfo_label=attB-P crossover nucleotides
                     /ApEinfo_fwdcolor=#009cff
                     /ApEinfo_revcolor=#009cff
     misc_feature    370..370
                     /label=attP-attB crossover nucleotides(1)
                     /ApEinfo_label=attP-attB crossover nucleotides
                     /ApEinfo_fwdcolor=#009cff
                     /ApEinfo_revcolor=#009cff
     CDS             complement(3238..3897)
                     /label=AmpR
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
     misc_recomb     complement(455..488)
                     /label=LoxP
                     /ApEinfo_fwdcolor=steel blue
                     /ApEinfo_revcolor=orange
     misc_feature    4096..4194
                     /label=ampR promoter
                     /ApEinfo_fwdcolor=#fffcc1
                     /ApEinfo_revcolor=#fffcc1
     misc_feature    3235..4095
                     /label=ampR
                     /ApEinfo_fwdcolor=#fffa4a
                     /ApEinfo_revcolor=#fffa4a
     rep_origin      2547..3237
                     /label=ori (pJB8)
                     /ApEinfo_fwdcolor=#808080
                     /ApEinfo_revcolor=#808080
ORIGIN
        1 CATGATGAAA TAACATAAGG TGGTCCCGTC GGCAAGAGAC ATCCACTTAA CGTATGCTTG
       61 CAATAAGTGC GAGTGAAAGG AATAGTATTC TGAGTGTCGT ATTGAGTCTG AGTGAGACAG
      121 CGATATGATT GTTGATTAAC CCTTAGCATG TCCGTGGGGT TTGAATTAAC TCATAATATT
      181 AATTAGACGA AATTATTTTT AAAGTTTTAT TTTTAATAAT TTGCGAGTAC GCAAAGCTAG
      241 AGTTGTTGGT TGGCACACCA CAAATATACT GTTGCCGAGC ACAATTCCGC TCGAATTGCC
      301 GGCTGTACAC TTAAGAAGTT CCTACTGCAG GTAATGAATT GCCGCGGTGC GGGTGCCAGG
      361 GCGTGCCCTT GGGCTCCCCG GGCGCGTACT CCACGAATTC TTGCATGCAA TGCGGCCGCT
      421 AGCAGATCTA CTAGTCTCGA GGCGCGCCGG TACCATAACT TCGTATAATG TATGCTATAC
      481 GAAGTTATCG TACGGGATCT AATTCAATTA GAGACTAATT CAATTAGAGC TAATTCAATT
      541 AGGATCCAAG CTTATCGATT TCGAACCCTC GACCGCCGGA GTATAAATAG AGGCGCTTCG
      601 TCTACGGAGC GACAATTCAA TTCAAACAAG CAAAGTGAAC ACGTCGCTAA GCGAAAGCTA
      661 AGCAAATAAA CAAGCGCAGC TGAACAAGCT AAACAATCGG CTCGAAGCCG GTCGCCACCA
      721 TGGCCTCCTC CGAGGACGTC ATCAAGGAGT TCATGCGCTT CAAGGTGCGC ATGGAGGGCT
      781 CCGTGAACGG CCACGAGTTC GAGATCGAGG GCGAGGGCGA GGGCCGCCCC TACGAGGGCA
      841 CCCAGACCGC CAAGCTGAAG GTGACCAAGG GCGGCCCCCT GCCCTTCGCC TGGGACATCC
      901 TGTCCCCCCA GTTCCAGTAC GGCTCCAAGG TGTACGTGAA GCACCCCGCC GACATCCCCG
      961 ACTACAAGAA GCTGTCCTTC CCCGAGGGCT TCAAGTGGGA GCGCGTGATG AACTTCGAGG
     1021 ACGGCGGCGT GGTGACCGTG ACCCAGGACT CCTCCCTCCA GGACGGCTCC TTCATCTACA
     1081 AGGTGAAGTT CATCGGCGTG AACTTCCCCT CCGACGGCCC CGTAATGCAG AAGAAGACTA
     1141 TGGGCTGGGA GGCGTCCACC GAGCGCCTGT ACCCCCGCGA CGGCGTGCTG AAGGGCGAGA
     1201 TCCACAAGGC CCTGAAGCTG AAGGACGGCG GCCACTACCT GGTGGAGTTC AAGTCCATCT
     1261 ACATGGCCAA GAAGCCCGTG CAGCTGCCCG GCTACTACTA CGTGGACTCC AAGCTGGACA
     1321 TCACCTCCCA CAACGAGGAC TACACCATCG TGGAGCAGTA CGAGCGCGCC GAGGGCCGCC
     1381 ACCACCTGTT CCTGTAGGGG CCGCGACTCT AGATCATAAT CAGCCATACC ACATTTGTAG
     1441 AGGTTTTACT TGCTTTAAAA AACCTCCCAC ACCTCCCCCT GAACCTGAAA CATAAAATGA
     1501 ATGCAATTGT TGTTGTTAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA
     1561 GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA
     1621 AACTCATCAA TGTATCTTAA GATCCAAACC TAGTGGCTGC TGCTCTAAAC GACGCATTTC
     1681 GTACTCCAAA GTACGAATTT TTTCCCTCAA GCTCTTATTT TCATTAAACA ATGAACAGGA
     1741 CCTAACGCAC AGTCACGTTA TTGTTTACAT AAATGATTTT TTTTACTATT CAAACTTACT
     1801 CTGTTTGTGT ACTCCCACTG GTATAGCCTT CTTTTATCTT TTCTGGTTCA GGCTCTATCA
     1861 CTTTACTAGG TACGGCATCT GCGTTGAGTC GCCTCCTTTT AAATGTCTGA CCTTTTGCAG
     1921 GTGCAGCCTT CCACTGCGAA TCATTAAAGT GGGTATCACA AATTTGGGAG TTTTCACCAA
     1981 GGCTGCACCC AAGGCTCTGC TCCCACAATT TTCTCTTAAT AGCACACTTC GGCACGTGAA
     2041 TTAATTTTAC TCCAGTCACA GCTTTGCAGC AAAATTTGCA ATATTTCATT TTTTTTTATT
     2101 CCACGTAAGG GTTAATGTTT TCAAAAAAAA ATTCGTCCGC ACACAACCTT TCCTCTCAAC
     2161 AAGCAAACGT GCACTGAATT TAAGTGTATA CTTCGGTAAG CTTCGGCTAT CGACGGGACC
     2221 ACCTTATGTT ATTTCATCAT GGGCCAGACC CACGTAGTCC AGCGGCAGAT CGGCGGCGGA
     2281 GAAGTTAAGC GTCTCCAGGA TGACCTTGCC CGAACTGGGG CACGTGGTGT TCGACGATGT
     2341 GCAGCTAATT TCGCCCGGCT CCACGTCCGC CCATTGGTTA ATCAGCAGAC CCTCGTTGGC
     2401 GTAACGGAAC CATGAGAGGT ACGACAACCA TTTGAGGTAT ACTGGCACCG AGCCCGAGTT
     2461 CAAGAAGAAG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG
     2521 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG
     2581 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT
     2641 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT
     2701 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG
     2761 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT
     2821 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT
     2881 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT
     2941 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC
     3001 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC
     3061 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG
     3121 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA
     3181 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA
     3241 ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC
     3301 CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC
     3361 TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC
     3421 AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT
     3481 TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT
     3541 TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC
     3601 CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG
     3661 CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT
     3721 TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC
     3781 TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG
     3841 CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT
     3901 TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC
     3961 GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC
     4021 TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA
     4081 ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG
     4141 TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG
     4201 CACATTTCCC CGAAAAGTGC CACCTGCGAA GTCTTAGAGC CAGATATGCG AGCACCCGGA
     4261 AGCTCACGAT GAGAATGGCC AGAC   
//
