LOCUS       pR0199_Cas13-B01_His6-T 9759 bp ds-DNA     circular     14-MAY-2017
DEFINITION  .
SOURCE      synthetic DNA construct                                            
  ORGANISM  recombinant plasmid                                                
FEATURES             Location/Qualifiers
     misc_feature    2541..2541
                     /label="replaced to remove BsaI site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    4310..7993
                     /label="BsaI site"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    4018..4309
                     /label="Sumo"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3869..4017
                     /label="6x His tag/ Twin Strep"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     3778..3797
                     /label="T7"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_binding    3797..3819
                     /label="LacO"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2444..2748
                     /label="GibsonInsert2"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(2403..3062)
                     /label="AmpR"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    4315..7986
                     /label="B-01"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      complement(1623..2305)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    4280..8023
                     /label="Gibsonpiece1"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
ORIGIN
        1 accaaacgtt tcggcgagaa gcaggccatt atcgccggca tggcggccga cgcgctgggc
       61 tacgtcttgc tggcgttcgc gacgcgaggc tggatggcct tccccattat gattcttctc
      121 gcttccggcg gcatcgggat gcccgcgttg caggccatgc tgtccaggca ggtagatgac
      181 gaccatcagg gacagcttca aggatcgctc gcggctctta ccagcctaac ttcgatcact
      241 ggaccgctga tcgtcacggc gatttatgcc gcctcggcga gcacatggaa cgggttggca
      301 tggattgtag gcgccgccct ataccttgtc tgcctccccg cgttgcgtcg cggtgcatgg
      361 agccgggcca cctcgacctg aatggaagcc ggcggcacct cgctaacgga ttcaccactc
      421 caagaattgg agccaatcaa ttcttgcgga gaactgtgaa tgcgcaaacc aacccttggc
      481 agaacatatc catcgcgtcc gccatctcca gcagccgcac gcggcgcatc tcgggcagcg
      541 ttgggtcctg gccacgggtg cgcatgatcg tgctcctgtc gttgaggacc cggctaggct
      601 ggcggggttg ccttactggt tagcagaatg aatcaccgat acgcgagcga acgtgaagcg
      661 actgctgctg caaaacgtct gcgacctgag caacaacatg aatggtcttc ggtttccgtg
      721 tttcgtaaag tctggaaacg cggaagtcag cgccctgcac cattatgttc cggatctgca
      781 tcgcaggatg ctgctggcta ccctgtggaa cacctacatc tgtattaacg aagcgctggc
      841 attgaccctg agtgattttt ctctggtccc gccgcatcca taccgccagt tgtttaccct
      901 cacaacgttc cagtaaccgg gcatgttcat catcagtaac ccgtatcgtg agcatcctct
      961 ctcgtttcat cggtatcatt acccccatga acagaaatcc cccttacacg gaggcatcag
     1021 tgaccaaaca ggaaaaaacc gcccttaaca tggcccgctt tatcagaagc cagacattaa
     1081 cgcttctgga gaaactcaac gagctggacg cggatgaaca ggcagacatc tgtgaatcgc
     1141 ttcacgacca cgctgatgag ctttaccgca gctgcctcgc gcgtttcggt gatgacggtg
     1201 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     1261 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
     1321 tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca
     1381 gattgtactg agagtgcacc atatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     1441 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     1501 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     1561 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     1621 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     1681 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     1741 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     1801 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     1861 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     1921 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     1981 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     2041 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     2101 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     2161 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     2221 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     2281 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     2341 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     2401 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     2461 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     2521 agtgctgcaa tgataccgcg tgacccacgc tcaccggctc cagatttatc agcaataaac
     2581 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     2641 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     2701 gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     2761 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     2821 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     2881 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     2941 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     3001 tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     3061 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     3121 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     3181 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     3241 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     3301 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     3361 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     3421 ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaagaacc gggatctcga
     3481 cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg
     3541 agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc
     3601 acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc
     3661 cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg
     3721 gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaattaa
     3781 tacgactcac tataggggaa ttgtgagcgg ataacaattc ccctctagaa ataattttgt
     3841 ttaactttaa gaaggagata taccatgggc agcagccatc atcatcatca tcacagcagc
     3901 ggcctggtgc cgcgcggcag ccatatggct agctggagcc atccgcagtt tgaaaaaggt
     3961 ggtggtagcg gtggtggttc aggtggtagt gcatggtcac accctcagtt tgagaaaatg
     4021 tcggactcag aagtcaatca agaagctaag ccagaggtca agccagaagt caagcctgag
     4081 actcacatca atttaaaggt gtccgatgga tcttcagaga tcttcttcaa gatcaaaaag
     4141 accactcctt taagaaggct gatggaagcg ttcgctaaaa gacagggtaa ggaaatggac
     4201 tccttaagat tcttgtacga cggtattaga atccaagctg atcagacccc tgaagatttg
     4261 gacatggagg ataacgatat tattgaggct cacagagaac agattggtgG ATCTatggag
     4321 aacaagacct ccctgggcaa caatatctac tataatccct tcaagcccca ggataagagc
     4381 tacttcgccg gctacttcaa cgccgccatg gagaataccg actccgtgtt ccgggagctg
     4441 ggcaagagac tgaagggcaa ggagtacaca tctgagaact tctttgacgc catctttaag
     4501 gagaatatct ctctggtgga gtacgagcgg tatgtgaagc tgctgagcga ttatttcccc
     4561 atggcccggc tgctggacaa gaaggaggtg cctatcaagg agagaaagga gaacttcaag
     4621 aagaacttca agggcatcat caaggccgtg cgggatctga gaaactttta cacccacaag
     4681 gagcacggcg aggtggagat cacagacgag atcttcggcg tgctggatga gatgctgaag
     4741 tccaccgtgc tgacagtgaa gaagaagaag gtgaaaaccg acaagacaaa ggagatcctg
     4801 aagaagtcta tcgagaagca gctggatatc ctgtgccaga agaagctgga gtacctgagg
     4861 gacaccgccc gcaagatcga ggagaagcgg agaaaccaga gggagagggg agagaaggag
     4921 ctggtggcac cttttaagta ttctgataag cgggacgacc tgatcgccgc catctacaat
     4981 gacgccttcg acgtgtatat cgacaagaag aaggattccc tgaaggagag ctccaaggcc
     5041 aagtacaaca caaagtctga ccctcagcag gaggagggcg atctgaagat cccaatcagc
     5101 aagaatggcg tggtgttcct gctgtccctg tttctgacca agcaggagat ccacgccttc
     5161 aagagcaaga tcgccggctt taaggccaca gtgatcgacg aggccaccgt gtccgaggcc
     5221 acagtgtctc acggcaagaa cagcatctgt ttcatggcca cccacgagat ctttagccac
     5281 ctggcctaca agaagctgaa gaggaaggtg cgcacagccg agatcaacta cggcgaggcc
     5341 gagaatgccg agcagctgtc cgtgtatgcc aaggagacac tgatgatgca gatgctggac
     5401 gagctgtcta aggtgcctga tgtggtgtac cagaacctga gcgaggacgt gcagaaaacc
     5461 ttcatcgagg actggaatga gtatctgaag gagaacaatg gcgacgtggg cacaatggag
     5521 gaggagcagg tcatccaccc agtgatccgg aagagatacg aggataagtt caactatttt
     5581 gccatcaggt tcctggacga gttcgcccag tttcctaccc tgcgctttca ggtgcacctg
     5641 ggcaactacc tgcacgattc ccggccaaag gagaatctga tctctgacag gcgcatcaag
     5701 gagaagatca cagtgtttgg cagactgtcc gagctggagc acaagaaggc cctgttcatc
     5761 aagaacaccg agacaaatga ggatagggag cactactggg agatcttccc caaccctaat
     5821 tatgactttc caaaggagaa catctccgtg aatgacaagg attttcccat cgccggctct
     5881 atcctggatc gcgagaagca gcctgtggcc ggcaagatcg gcatcaaggt gaagctgctg
     5941 aaccagcagt acgtgtctga ggtggacaag gccgtgaagg cccaccagct gaagcagcgg
     6001 aaggcctcta agcccagcat ccagaatatc atcgaggaga tcgtgcccat caacgagagc
     6061 aatcctaagg aggcaatcgt gttcggagga cagcctaccg cctacctgtc tatgaacgat
     6121 atccacagca tcctgtatga gttctttgac aagtgggaga agaagaagga gaagctggag
     6181 aagaagggcg agaaggagct gagaaaggag atcggcaagg agctggagaa gaagatcgtg
     6241 ggcaagatcc aggcccagat ccagcagatc atcgacaagg ataccaacgc caagatcctg
     6301 aagccctacc aggacggcaa tagcacagcc atcgataagg agaagctgat caaggacctg
     6361 aagcaggagc agaacatcct gcagaagctg aaggatgagc agaccgtgag ggagaaggag
     6421 tacaatgact tcatcgccta tcaggataag aaccgcgaga tcaataaggt gagggatcgc
     6481 aaccacaagc agtacctgaa ggacaatctg aagaggaagt atcctgaggc cccagccagg
     6541 aaggaggtgc tgtactatcg cgagaagggc aaggtggccg tgtggctggc caacgatatc
     6601 aagcggttca tgcctaccga ctttaagaat gagtggaagg gcgagcagca ctccctgctg
     6661 cagaagtctc tggcctacta tgagcagtgc aaggaggagc tgaagaacct gctgccagag
     6721 aaggtgttcc agcacctgcc ctttaagctg ggcggctact tccagcagaa gtacctgtat
     6781 cagttttaca cctgctatct ggacaagagg ctggagtata tcagcggcct ggtgcagcag
     6841 gccgagaact tcaagtccga gaataaggtg tttaagaagg tggagaacga gtgtttcaag
     6901 tttctgaaga agcagaatta cacacacaag gagctggatg cccgggtgca gtccatcctg
     6961 ggctatccaa tcttcctgga gagaggcttt atggacgaga agcccaccat catcaagggc
     7021 aagacattca agggcaacga ggccctgttc gccgattggt ttcgctacta taaggagtac
     7081 cagaacttcc agacctttta cgacacagag aattatccac tggtggagct ggagaagaag
     7141 caggccgatc ggaagagaaa gaccaagatc tatcagcaga agaagaatga cgtgtttaca
     7201 ctgctgatgg ccaagcacat cttcaagagc gtgttcaagc aggacagcat cgatcagttc
     7261 tccctggagg acctgtacca gagccgggag gagagactgg gaaaccagga gagggcaagg
     7321 cagaccggcg agaggaacac aaattatatc tggaataaga ccgtggacct gaagctgtgc
     7381 gatggcaaga tcacagtgga gaacgtgaag ctgaagaatg tgggcgattt catcaagtac
     7441 gagtatgacc agcgggtgca ggcctttctg aagtacgagg agaacatcga gtggcaggcc
     7501 ttcctgatca aggagagcaa ggaggaggag aattacccct atgtggtgga gagagagatc
     7561 gagcagtacg agaaggtgcg gagagaggag ctgctgaagg aggtgcacct gatcgaggag
     7621 tatatcctgg agaaggtgaa ggataaagaa atcctgaaga agggcgacaa ccagaacttc
     7681 aagtactata tcctgaacgg cctgctgaag cagctgaaga atgaggatgt ggagtcctac
     7741 aaggtgttca acctgaatac cgagccagag gatgtgaaca tcaatcagct gaagcaggag
     7801 gccaccgacc tggagcagaa ggccttcgtg ctgacatata tccggaacaa gtttgcccac
     7861 aatcagctgc ccaagaagga gttctgggac tactgtcagg agaagtatgg caagatcgag
     7921 aaggagaaaa cctacgccga gtatttcgcc gaggtgttta agaaggagaa ggaggccctg
     7981 atcaagTAAg gCCgcactcg aggcccgaaa ggaagctgag ttggctgctg ccaccgctga
     8041 gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa
     8101 aggaggaact atatccggat atcccgcaag aggcccggca gtaccggcat aaccaagcct
     8161 atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc
     8221 atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta
     8281 tcgatgataa gctgtcaaac atgagaaaca ccatcgaatg gcgcaaaacc tttcgcggta
     8341 tggcatgata gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgt
     8401 tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc gtggtgaacc
     8461 aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg gcggagctga
     8521 attacattcc caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg ctgattggcg
     8581 ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc
     8641 gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag
     8701 cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg atcattaact
     8761 atccgctgga tgaccaggat gccattgctg tggaagctgc ctgcactaat gttccggcgt
     8821 tatttcttga tgtctctgac cagacaccca tcaacagtat tattttctcc catgaagacg
     8881 gtacgcgact gggcgtggag catctggtcg cattgggtca ccagcaaatc gcgctgttag
     8941 cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat aaatatctca
     9001 ctcgcaatca aattcagccg atagcggaac gggaaggcga ctggagtgcc atgtccggtt
     9061 ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca
     9121 acgatcagat ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg
     9181 cggatatctc ggtagtggga tacgacgata ccgaagacag ctcatgttat atcccgccgt
     9241 taaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc
     9301 aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa
     9361 gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     9421 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     9481 aatgtaagtt agctcactca ttaggcaccg ggatctcgac cgatgccctt gagagccttc
     9541 aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact
     9601 gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctctgggt cattttcggc
     9661 gaggaccgct ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt attcggaatc
     9721 ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcc
//