LOCUS       pR0199_Cas13-B02_His6-T 9465 bp ds-DNA     circular     14-MAY-2017
DEFINITION  .
SOURCE      synthetic DNA construct                                            
  ORGANISM  recombinant plasmid                                                
FEATURES             Location/Qualifiers
     rep_origin      complement(1623..2305)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_binding    3797..3819
                     /label="LacO"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             complement(2403..3062)
                     /label="AmpR"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2444..2748
                     /label="GibsonInsert2"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    4280..7729
                     /label="Gibsonpiece1"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    4315..7692
                     /label="B-02"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2541..2541
                     /label="replaced to remove BsaI site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    4310..7699
                     /label="BsaI site"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    4018..4309
                     /label="Sumo"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     3778..3797
                     /label="T7"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    3869..4017
                     /label="6x His tag/ Twin Strep"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
ORIGIN
        1 accaaacgtt tcggcgagaa gcaggccatt atcgccggca tggcggccga cgcgctgggc
       61 tacgtcttgc tggcgttcgc gacgcgaggc tggatggcct tccccattat gattcttctc
      121 gcttccggcg gcatcgggat gcccgcgttg caggccatgc tgtccaggca ggtagatgac
      181 gaccatcagg gacagcttca aggatcgctc gcggctctta ccagcctaac ttcgatcact
      241 ggaccgctga tcgtcacggc gatttatgcc gcctcggcga gcacatggaa cgggttggca
      301 tggattgtag gcgccgccct ataccttgtc tgcctccccg cgttgcgtcg cggtgcatgg
      361 agccgggcca cctcgacctg aatggaagcc ggcggcacct cgctaacgga ttcaccactc
      421 caagaattgg agccaatcaa ttcttgcgga gaactgtgaa tgcgcaaacc aacccttggc
      481 agaacatatc catcgcgtcc gccatctcca gcagccgcac gcggcgcatc tcgggcagcg
      541 ttgggtcctg gccacgggtg cgcatgatcg tgctcctgtc gttgaggacc cggctaggct
      601 ggcggggttg ccttactggt tagcagaatg aatcaccgat acgcgagcga acgtgaagcg
      661 actgctgctg caaaacgtct gcgacctgag caacaacatg aatggtcttc ggtttccgtg
      721 tttcgtaaag tctggaaacg cggaagtcag cgccctgcac cattatgttc cggatctgca
      781 tcgcaggatg ctgctggcta ccctgtggaa cacctacatc tgtattaacg aagcgctggc
      841 attgaccctg agtgattttt ctctggtccc gccgcatcca taccgccagt tgtttaccct
      901 cacaacgttc cagtaaccgg gcatgttcat catcagtaac ccgtatcgtg agcatcctct
      961 ctcgtttcat cggtatcatt acccccatga acagaaatcc cccttacacg gaggcatcag
     1021 tgaccaaaca ggaaaaaacc gcccttaaca tggcccgctt tatcagaagc cagacattaa
     1081 cgcttctgga gaaactcaac gagctggacg cggatgaaca ggcagacatc tgtgaatcgc
     1141 ttcacgacca cgctgatgag ctttaccgca gctgcctcgc gcgtttcggt gatgacggtg
     1201 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     1261 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
     1321 tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca
     1381 gattgtactg agagtgcacc atatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     1441 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     1501 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     1561 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     1621 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     1681 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     1741 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     1801 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     1861 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     1921 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     1981 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     2041 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     2101 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     2161 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     2221 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     2281 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     2341 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     2401 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     2461 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     2521 agtgctgcaa tgataccgcg tgacccacgc tcaccggctc cagatttatc agcaataaac
     2581 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     2641 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     2701 gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     2761 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     2821 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     2881 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     2941 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     3001 tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     3061 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     3121 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     3181 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     3241 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     3301 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     3361 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     3421 ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaagaacc gggatctcga
     3481 cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg
     3541 agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc
     3601 acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc
     3661 cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg
     3721 gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaattaa
     3781 tacgactcac tataggggaa ttgtgagcgg ataacaattc ccctctagaa ataattttgt
     3841 ttaactttaa gaaggagata taccatgggc agcagccatc atcatcatca tcacagcagc
     3901 ggcctggtgc cgcgcggcag ccatatggct agctggagcc atccgcagtt tgaaaaaggt
     3961 ggtggtagcg gtggtggttc aggtggtagt gcatggtcac accctcagtt tgagaaaatg
     4021 tcggactcag aagtcaatca agaagctaag ccagaggtca agccagaagt caagcctgag
     4081 actcacatca atttaaaggt gtccgatgga tcttcagaga tcttcttcaa gatcaaaaag
     4141 accactcctt taagaaggct gatggaagcg ttcgctaaaa gacagggtaa ggaaatggac
     4201 tccttaagat tcttgtacga cggtattaga atccaagctg atcagacccc tgaagatttg
     4261 gacatggagg ataacgatat tattgaggct cacagagaac agattggtgG ATCTatggag
     4321 gacgataaga aaaccacaga tagcatcaga tacgagctga aggacaagca cttctgggcc
     4381 gcctttctga atctggccag gcacaacgtg tatatcaccg tgaatcacat caacaagatc
     4441 ctggaggagg gcgagatcaa ccgcgatggc tacgagacaa ccctgaagaa tacatggaac
     4501 gagatcaagg atatcaataa gaaggaccgc ctgtccaagc tgatcatcaa gcacttcccc
     4561 tttctggagg ccgccaccta ccggctgaac cctacagata ccacaaagca gaaggaggag
     4621 aagcaggcag aggcacagtc cctggagtct ctgcggaaga gcttcttcgt gttcatctac
     4681 aagctgaggg acctgcgcaa tcactactct cactataagc acagcaagag cctggagcgg
     4741 cccaagttcg aggagggcct gctggagaag atgtataaca tctttaacgc cagcatcagg
     4801 ctggtgaagg aggattacca gtataataag gacatcaacc ctgacgagga tttcaagcac
     4861 ctggatcgca ccgaggagga gttcaactac tacttcacca aggacaatga gggcaacatc
     4921 acagagagcg gcctgctgtt cttcgtgagc ctgttcctgg agaagaagga tgccatctgg
     4981 atgcagcaga agctgcgcgg cttcaaggac aatcgggaga acaagaagaa gatgaccaac
     5041 gaggtgtttt gcaggagccg catgctgctg cctaagctgc gcctgcagtc cacccagaca
     5101 caggattgga ttctgctgga catgctgaat gagctgatcc ggtgtccaaa gtccctgtac
     5161 gagcggctga gagaggagga ccgcgagaag ttccgggtgc ccatcgagat cgccgacgag
     5221 gattatgacg ccgagcagga gccttttaag aacaccctgg tgagacacca ggataggttc
     5281 ccatactttg ccctgagata cttcgactat aatgagatct tcaccaacct gaggtttcag
     5341 atcgatctgg gcacatacca cttttctatc tataagaagc agatcggcga ctacaaggag
     5401 agccaccacc tgacccacaa gctgtatggc ttcgagagaa tccaggagtt tacaaagcag
     5461 aaccgccccg atgagtggcg gaagttcgtg aaaaccttca actcttttga gacaagcaag
     5521 gagccataca tccccgagac aacacctcac tatcacctgg agaaccagaa gatcggcatc
     5581 aggtttcgca acgataatga caagatctgg cccagcctga aaaccaacag cgagaagaac
     5641 gagaagtcta agtacaagct ggacaagagc ttccaggccg aggcctttct gagcgtgcac
     5701 gagctgctgc caatgatgtt ctactatctg ctgctgaaaa ccgagaacac agataacgac
     5761 aatgagatcg agacaaagaa gaaggagaac aagaatgata agcaggagaa gcacaagatc
     5821 gaggagatca tcgagaacaa gatcaccgag atctacgccc tgtatgacac attcgccaat
     5881 ggcgagatca agtccatcga tgagctggag gagtactgca agggcaagga catcgagatc
     5941 ggccacctgc ccaagcagat gatcgccatc ctgaaggacg agcacaaagt gatggccacc
     6001 gaggccgaga gaaagcagga ggagatgctg gtggatgtgc agaagagcct ggagtccctg
     6061 gacaatcaga tcaacgagga gatcgagaat gtggagagaa agaacagctc cctgaagtcc
     6121 ggcaagatcg cctcttggct ggtgaacgat atgatgaggt tccagcctgt gcagaaggac
     6181 aacgagggca agccactgaa caattccaag gccaattcta ccgagtatca gctgctgcag
     6241 cgcacactgg ccttctttgg ctccgagcac gagcggctgg ccccttactt caagcagacc
     6301 aagctgatcg agtctagcaa tccccaccct tttctgaagg atacagagtg ggagaagtgt
     6361 aacaatatcc tgagcttcta caggtcctat ctggaggcca agaagaactt tctggagtct
     6421 ctgaagccag aggactggga gaagaatcag tatttcctga agctgaagga gcctaagacc
     6481 aagccaaaga cactggtgca gggctggaag aatggcttca acctgccacg cggcatcttt
     6541 accgagccca tcagaaagtg gtttatgaag cacagggaga acatcacagt ggcagagctg
     6601 aagagggtgg gcctggtggc caaagtgatc cccctgttct ttagcgagga gtacaaggat
     6661 tccgtgcagc ctttctacaa ttatcacttt aacgtgggca acatcaataa gccagacgag
     6721 aagaatttcc tgaactgcga ggagcggaga gagctgctgc ggaagaagaa ggatgagttt
     6781 aagaagatga ccgacaagga gaaggaggag aacccctctt atctggagtt caagagctgg
     6841 aataagtttg agcgggagct gagactggtg aggaaccagg atatcgtgac atggctgctg
     6901 tgcatggagc tgttcaacaa gaagaagatc aaggagctga atgtggagaa gatctacctg
     6961 aagaacatca ataccaacac cacaaagaag gagaagaata cagaggagaa gaacggcgag
     7021 gagaagaata tcaaggagaa gaacaatatc ctgaacagaa tcatgcccat gaggctgcct
     7081 atcaaggtgt atggcagaga gaatttcagc aagaacaaga agaagaagat caggcgcaac
     7141 accttcttta ccgtgtatat cgaggagaag ggcaccaagc tgctgaagca gggcaacttc
     7201 aaggccctgg agagggaccg gagactggga ggcctgttca gcttcgtgaa aaccccctct
     7261 aaggccgagt ctaagagcaa cacaatctct aagctgcgcg tggagtacga gctgggcgag
     7321 tatcagaagg cccggatcga gatcatcaag gatatgctgg ccctggagaa aaccctgatc
     7381 gacaagtata atagcctgga taccgacaat ttcaacaaga tgctgacaga ttggctggag
     7441 ctgaagggcg agcctgacaa ggccagcttc cagaacgatg tggacctgct gatcgccgtg
     7501 cggaacgcct tttcccacaa tcagtaccca atgcggaata gaatcgcctt cgccaatatc
     7561 aacccctttt ccctgtcctc tgccaacacc tctgaggaga agggcctggg catcgccaat
     7621 cagctgaagg acaagaccca caagacaatc gagaagatca tcgagatcga gaagcccatc
     7681 gagacaaagg agTAAggCCg cactcgaggc ccgaaaggaa gctgagttgg ctgctgccac
     7741 cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt
     7801 gctgaaagga ggaactatat ccggatatcc cgcaagaggc ccggcagtac cggcataacc
     7861 aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta
     7921 gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa
     7981 agcttatcga tgataagctg tcaaacatga gaaacaccat cgaatggcgc aaaacctttc
     8041 gcggtatggc atgatagcgc ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag
     8101 taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg
     8161 tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg
     8221 agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga
     8281 ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta
     8341 aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg
     8401 tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca
     8461 ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc
     8521 cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg
     8581 aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc
     8641 tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat
     8701 atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt
     8761 ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg
     8821 ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg
     8881 ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc
     8941 cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct
     9001 tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg
     9061 tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     9121 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     9181 gcaattaatg taagttagct cactcattag gcaccgggat ctcgaccgat gcccttgaga
     9241 gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt
     9301 atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt
     9361 ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc
     9421 ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgcc
//