LOCUS       pR0199_Cas13-B06_His6-T 9372 bp ds-DNA     circular     08-JUN-2017
DEFINITION  .
SOURCE      synthetic DNA construct                                            
  ORGANISM  recombinant plasmid                                                
FEATURES             Location/Qualifiers
     CDS             complement(2403..3062)
                     /label="AmpR"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    7435..7455
                     /label="B6 Pair3 F"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    4310..7606
                     /label="BsaI site"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer_bind     3778..3797
                     /label="T7"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    4280..7636
                     /label="Gibsonpiece1"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(7435..7455)
                     /label="B6 Pair2 R"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_binding    3797..3819
                     /label="LacO"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    complement(4739..4768)
                     /label="B6 Pair1 R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    4739..4768
                     /label="B6 Pair2 F"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    complement(7573..7599)
                     /label="B6 Pair3 R"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    4018..4309
                     /label="Sumo"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    4315..7599
                     /label="B-06"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     rep_origin      complement(1623..2305)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2444..2748
                     /label="GibsonInsert2"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2541..2541
                     /label="replaced to remove BsaI site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    4315..4344
                     /label="B6 Pair1 F"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    3869..4017
                     /label="6x His tag/ Twin Strep"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
ORIGIN
        1 accaaacgtt tcggcgagaa gcaggccatt atcgccggca tggcggccga cgcgctgggc
       61 tacgtcttgc tggcgttcgc gacgcgaggc tggatggcct tccccattat gattcttctc
      121 gcttccggcg gcatcgggat gcccgcgttg caggccatgc tgtccaggca ggtagatgac
      181 gaccatcagg gacagcttca aggatcgctc gcggctctta ccagcctaac ttcgatcact
      241 ggaccgctga tcgtcacggc gatttatgcc gcctcggcga gcacatggaa cgggttggca
      301 tggattgtag gcgccgccct ataccttgtc tgcctccccg cgttgcgtcg cggtgcatgg
      361 agccgggcca cctcgacctg aatggaagcc ggcggcacct cgctaacgga ttcaccactc
      421 caagaattgg agccaatcaa ttcttgcgga gaactgtgaa tgcgcaaacc aacccttggc
      481 agaacatatc catcgcgtcc gccatctcca gcagccgcac gcggcgcatc tcgggcagcg
      541 ttgggtcctg gccacgggtg cgcatgatcg tgctcctgtc gttgaggacc cggctaggct
      601 ggcggggttg ccttactggt tagcagaatg aatcaccgat acgcgagcga acgtgaagcg
      661 actgctgctg caaaacgtct gcgacctgag caacaacatg aatggtcttc ggtttccgtg
      721 tttcgtaaag tctggaaacg cggaagtcag cgccctgcac cattatgttc cggatctgca
      781 tcgcaggatg ctgctggcta ccctgtggaa cacctacatc tgtattaacg aagcgctggc
      841 attgaccctg agtgattttt ctctggtccc gccgcatcca taccgccagt tgtttaccct
      901 cacaacgttc cagtaaccgg gcatgttcat catcagtaac ccgtatcgtg agcatcctct
      961 ctcgtttcat cggtatcatt acccccatga acagaaatcc cccttacacg gaggcatcag
     1021 tgaccaaaca ggaaaaaacc gcccttaaca tggcccgctt tatcagaagc cagacattaa
     1081 cgcttctgga gaaactcaac gagctggacg cggatgaaca ggcagacatc tgtgaatcgc
     1141 ttcacgacca cgctgatgag ctttaccgca gctgcctcgc gcgtttcggt gatgacggtg
     1201 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     1261 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
     1321 tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca
     1381 gattgtactg agagtgcacc atatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     1441 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     1501 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     1561 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     1621 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     1681 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     1741 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     1801 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     1861 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     1921 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     1981 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     2041 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     2101 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     2161 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     2221 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     2281 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     2341 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     2401 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     2461 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     2521 agtgctgcaa tgataccgcg tgacccacgc tcaccggctc cagatttatc agcaataaac
     2581 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     2641 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     2701 gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     2761 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     2821 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     2881 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     2941 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     3001 tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     3061 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     3121 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     3181 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     3241 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     3301 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     3361 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     3421 ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaagaacc gggatctcga
     3481 cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg
     3541 agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc
     3601 acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc
     3661 cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg
     3721 gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaattaa
     3781 tacgactcac tataggggaa ttgtgagcgg ataacaattc ccctctagaa ataattttgt
     3841 ttaactttaa gaaggagata taccatgggc agcagccatc atcatcatca tcacagcagc
     3901 ggcctggtgc cgcgcggcag ccatatggct agctggagcc atccgcagtt tgaaaaaggt
     3961 ggtggtagcg gtggtggttc aggtggtagt gcatggtcac accctcagtt tgagaaaatg
     4021 tcggactcag aagtcaatca agaagctaag ccagaggtca agccagaagt caagcctgag
     4081 actcacatca atttaaaggt gtccgatgga tcttcagaga tcttcttcaa gatcaaaaag
     4141 accactcctt taagaaggct gatggaagcg ttcgctaaaa gacagggtaa ggaaatggac
     4201 tccttaagat tcttgtacga cggtattaga atccaagctg atcagacccc tgaagatttg
     4261 gacatggagg ataacgatat tattgaggct cacagagaac agattggtgG ATCTatggag
     4321 aagcccctgc tgcctaacgt gtataccctg aagcacaagt tcttttgggg cgccttcctg
     4381 aatatcgccc ggcacaacgc ctttatcacc atctgccaca tcaatgagca gctgggcctg
     4441 aaaaccccca gcaacgacga taagatcgtg gacgtggtgt gcgagacatg gaacaatatc
     4501 ctgaacaatg accacgatct gctgaagaag tcccagctga ccgagctgat cctgaagcac
     4561 ttcccttttc tgacagccat gtgctaccac ccccctaaga aggagggcaa gaagaagggc
     4621 caccagaagg agcagcagaa ggagaaggag agcgaggcac agtcccaggc agaggccctg
     4681 aacccatcca agctgatcga ggccctggag atcctggtga atcagctgca ctctctgcgg
     4741 aactactata gccactataa gcacaagaag cccgacgccg agaaggatat cttcaagcac
     4801 ctgtataagg ccttcgacgc ctctctgaga atggtgaagg aggattataa ggcccacttc
     4861 accgtgaatc tgacaaggga ctttgcccac ctgaaccgca agggcaagaa caagcaggac
     4921 aatcccgact tcaacagata cagattcgag aaggatggct tctttaccga gtctggcctg
     4981 ctgttcttta caaatctgtt tctggacaag agggatgcct attggatgct gaagaaggtg
     5041 tccggcttca aggcctctca caagcagcgc gagaagatga ccacagaggt gttttgcagg
     5101 agccgcatcc tgctgcccaa gctgaggctg gagtcccgct acgaccacaa ccagatgctg
     5161 ctggatatgc tgtctgagct gagcagatgt cctaagctgc tgtatgagaa gctgagcgag
     5221 gagaataaga agcacttcca ggtggaggcc gacggctttc tggatgagat cgaggaggag
     5281 cagaacccat tcaaggacac cctgatccgg caccaggata gattccccta ctttgccctg
     5341 aggtatctgg acctgaatga gtccttcaag tctatccgct ttcaggtgga tctgggcaca
     5401 taccactatt gtatctacga caagaagatc ggcgatgagc aggagaagag gcacctgacc
     5461 cgcacactgc tgtccttcgg ccggctgcag gactttaccg agatcaacag accccaggag
     5521 tggaaggccc tgaccaagga cctggattac aaggagacat ccaatcagcc tttcatctct
     5581 aagaccacac cacactatca catcaccgac aacaagatcg gctttcggct gggcacaagc
     5641 aaggagctgt acccctccct ggagatcaag gatggcgcca atagaatcgc caagtaccct
     5701 tataactccg gcttcgtggc ccacgccttt atctctgtgc acgagctgct gcctctgatg
     5761 ttctaccagc acctgaccgg caagagcgag gacctgctga aggagacagt gcggcacatc
     5821 cagagaatct ataaggactt cgaggaggag cggatcaata ccatcgagga tctggagaag
     5881 gcaaaccagg gcagactgcc actgggagcc tttcccaagc agatgctggg cctgctgcag
     5941 aataagcagc ctgatctgtc cgagaaggcc aagatcaaga tcgagaagct gatcgccgag
     6001 acaaagctgc tgtctcacag gctgaacaca aagctgaaga gctccccaaa gctgggcaag
     6061 cggagagaga agctgatcaa gacaggcgtg ctggccgact ggctggtgaa ggatttcatg
     6121 cgctttcagc ccgtggccta cgacgcccag aatcagccta tcaagtctag caaggccaac
     6181 tccaccgagt tctggtttat caggcgcgcc ctggccctgt acggaggaga gaagaatagg
     6241 ctggagggct atttcaagca gaccaacctg atcggcaaca caaatccaca ccccttcctg
     6301 aacaagttta attggaaggc ctgcaggaat ctggtggatt tctaccagca gtatctggag
     6361 cagcgcgaga agtttctgga ggccatcaag aaccagccat gggagcccta ccagtattgc
     6421 ctgctgctga agatccctaa ggagaacagg aagaatctgg tgaagggatg ggagcaggga
     6481 ggaatctctc tgccacgggg cctgtttacc gaggccatca gagagacact gtctgaggac
     6541 ctgatgctga gcaagccaat ccgcaaggag atcaagaagc acggccgggt gggcttcatc
     6601 agcagagcca tcaccctgta ctttaaggag aagtatcagg ataagcacca gagcttctac
     6661 aatctgtcct ataagctgga ggcaaaggca ccactgctga agcgggagga gcactacgag
     6721 tattggcagc agaacaagcc tcagtctcca accgagagcc agaggctgga gctgcacaca
     6781 agcgaccgct ggaaggatta cctgctgtat aagagatggc agcacctgga gaagaagctg
     6841 cggctgtacc ggaatcagga cgtgatgctg tggctgatga ccctggagct gacaaagaac
     6901 cacttcaagg agctgaacct gaattatcac cagctgaagc tggagaatct ggccgtgaac
     6961 gtgcaggagg ccgatgccaa gctgaaccct ctgaatcaga ccctgcccat ggtgctgcct
     7021 gtgaaggtgt accctgccac agcctttggc gaggtgcagt accacaagac cccaatcagg
     7081 acagtgtata tccgcgagga gcacaccaag gccctgaaga tgggcaactt caaggccctg
     7141 gtgaaggacc ggagactgaa tggcctgttc tcttttatca aggaggagaa cgatacacag
     7201 aagcacccaa tcagccagct gcggctgagg cgcgagctgg agatctacca gtctctgaga
     7261 gtggacgcct tcaaggagac actgagcctg gaggagaagc tgctgaataa gcacacatcc
     7321 ctgtcctctc tggagaacga gtttagggcc ctgctggagg agtggaagaa ggagtatgcc
     7381 gccagctcca tggtgacaga cgagcacatc gccttcatcg ccagcgtgcg caatgccttc
     7441 tgccacaacc agtacccctt ctacaaggag gccctgcacg cccctatccc actgttcacc
     7501 gtggcccagc ccaccacaga ggagaaggat ggcctgggaa tcgcagaggc cctgctgaag
     7561 gtgctgaggg agtactgtga gatcgtgaag tcccagatcT AAggCCgcac tcgaggcccg
     7621 aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc
     7681 ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatcccgc
     7741 aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg
     7801 ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa
     7861 tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
     7921 acaccatcga atggcgcaaa acctttcgcg gtatggcatg atagcgcccg gaagagagtc
     7981 aattcagggt ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag tatgccggtg
     8041 tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct gcgaaaacgc
     8101 gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc gtggcacaac
     8161 aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg gccctgcacg
     8221 cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt gccagcgtgg
     8281 tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg cacaatcttc
     8341 tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag gatgccattg
     8401 ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct gaccagacac
     8461 ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg gagcatctgg
     8521 tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct gtctcggcgc
     8581 gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag ccgatagcgg
     8641 aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa atgctgaatg
     8701 agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg ggcgcaatgc
     8761 gcgccattac cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg
     8821 ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag gattttcgcc
     8881 tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag gcggtgaagg
     8941 gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg cccaatacgc
     9001 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     9061 gactggaaag cgggcagtga gcgcaacgca attaatgtaa gttagctcac tcattaggca
     9121 ccgggatctc gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg
     9181 cggggcatga ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga
     9241 caggtgccgg cagcgctctg ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg
     9301 atgatcggcc tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca agccttcgtc
     9361 actggtcccg cc
//