LOCUS       pR0199_Cas13-B12_His6-T 9357 bp ds-DNA     circular     08-JUN-2017
DEFINITION  .
SOURCE      synthetic DNA construct                                            
  ORGANISM  recombinant plasmid                                                
FEATURES             Location/Qualifiers
     misc_feature    3869..4017
                     /label="6x His tag/ Twin Strep"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    4315..4338
                     /label="B12 Pair1 F"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     CDS             complement(2403..3062)
                     /label="AmpR"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer_bind     3778..3797
                     /label="T7"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    complement(7476..7499)
                     /label="B12 Pair2 R"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    complement(4701..4723)
                     /label="B12 Pair1 R"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    7476..7499
                     /label="B12 Pair3 F"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(7560..7584)
                     /label="B12 Pair 3 R"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2541..2541
                     /label="replaced to remove BsaI site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    4701..4723
                     /label="B12 Pair2 F"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_binding    3797..3819
                     /label="LacO"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    4310..7591
                     /label="BsaI site"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    4280..7621
                     /label="Gibsonpiece1"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    4315..7584
                     /label="B-12"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     rep_origin      complement(1623..2305)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2444..2748
                     /label="GibsonInsert2"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    4018..4309
                     /label="Sumo"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
ORIGIN
        1 accaaacgtt tcggcgagaa gcaggccatt atcgccggca tggcggccga cgcgctgggc
       61 tacgtcttgc tggcgttcgc gacgcgaggc tggatggcct tccccattat gattcttctc
      121 gcttccggcg gcatcgggat gcccgcgttg caggccatgc tgtccaggca ggtagatgac
      181 gaccatcagg gacagcttca aggatcgctc gcggctctta ccagcctaac ttcgatcact
      241 ggaccgctga tcgtcacggc gatttatgcc gcctcggcga gcacatggaa cgggttggca
      301 tggattgtag gcgccgccct ataccttgtc tgcctccccg cgttgcgtcg cggtgcatgg
      361 agccgggcca cctcgacctg aatggaagcc ggcggcacct cgctaacgga ttcaccactc
      421 caagaattgg agccaatcaa ttcttgcgga gaactgtgaa tgcgcaaacc aacccttggc
      481 agaacatatc catcgcgtcc gccatctcca gcagccgcac gcggcgcatc tcgggcagcg
      541 ttgggtcctg gccacgggtg cgcatgatcg tgctcctgtc gttgaggacc cggctaggct
      601 ggcggggttg ccttactggt tagcagaatg aatcaccgat acgcgagcga acgtgaagcg
      661 actgctgctg caaaacgtct gcgacctgag caacaacatg aatggtcttc ggtttccgtg
      721 tttcgtaaag tctggaaacg cggaagtcag cgccctgcac cattatgttc cggatctgca
      781 tcgcaggatg ctgctggcta ccctgtggaa cacctacatc tgtattaacg aagcgctggc
      841 attgaccctg agtgattttt ctctggtccc gccgcatcca taccgccagt tgtttaccct
      901 cacaacgttc cagtaaccgg gcatgttcat catcagtaac ccgtatcgtg agcatcctct
      961 ctcgtttcat cggtatcatt acccccatga acagaaatcc cccttacacg gaggcatcag
     1021 tgaccaaaca ggaaaaaacc gcccttaaca tggcccgctt tatcagaagc cagacattaa
     1081 cgcttctgga gaaactcaac gagctggacg cggatgaaca ggcagacatc tgtgaatcgc
     1141 ttcacgacca cgctgatgag ctttaccgca gctgcctcgc gcgtttcggt gatgacggtg
     1201 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     1261 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
     1321 tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca
     1381 gattgtactg agagtgcacc atatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     1441 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     1501 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     1561 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     1621 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     1681 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     1741 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     1801 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     1861 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     1921 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     1981 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     2041 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     2101 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     2161 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     2221 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     2281 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     2341 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     2401 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     2461 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     2521 agtgctgcaa tgataccgcg tgacccacgc tcaccggctc cagatttatc agcaataaac
     2581 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     2641 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     2701 gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     2761 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     2821 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     2881 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     2941 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     3001 tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     3061 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     3121 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     3181 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     3241 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     3301 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     3361 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     3421 ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaagaacc gggatctcga
     3481 cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg
     3541 agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc
     3601 acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc
     3661 cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg
     3721 gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaattaa
     3781 tacgactcac tataggggaa ttgtgagcgg ataacaattc ccctctagaa ataattttgt
     3841 ttaactttaa gaaggagata taccatgggc agcagccatc atcatcatca tcacagcagc
     3901 ggcctggtgc cgcgcggcag ccatatggct agctggagcc atccgcagtt tgaaaaaggt
     3961 ggtggtagcg gtggtggttc aggtggtagt gcatggtcac accctcagtt tgagaaaatg
     4021 tcggactcag aagtcaatca agaagctaag ccagaggtca agccagaagt caagcctgag
     4081 actcacatca atttaaaggt gtccgatgga tcttcagaga tcttcttcaa gatcaaaaag
     4141 accactcctt taagaaggct gatggaagcg ttcgctaaaa gacagggtaa ggaaatggac
     4201 tccttaagat tcttgtacga cggtattaga atccaagctg atcagacccc tgaagatttg
     4261 gacatggagg ataacgatat tattgaggct cacagagaac agattggtgG ATCTatgaac
     4321 atccccgctc tggtggaaaa ccagaagaag tactttggca cctacagcgt gatggccatg
     4381 ctgaacgctc agaccgtgct ggaccacatc cagaaggtgg ccgatattga gggcgagcag
     4441 aacgagaaca acgagaatct gtggtttcac cccgtgatga gccacctgta caacgccaag
     4501 aacggctacg acaagcagcc cgagaaaacc atgttcatca tcgagcggct gcagagctac
     4561 ttcccattcc tgaagatcat ggccgagaac cagagagagt acagcaacgg caagtacaag
     4621 cagaaccgcg tggaagtgaa cagcaacgac atcttcgagg tgctgaagcg cgccttcggc
     4681 gtgctgaaga tgtacaggga cctgaccaac cactacaaga cctacgagga aaagctgaac
     4741 gacggctgcg agttcctgac cagcacagag caacctctga gcggcatgat caacaactac
     4801 tacacagtgg ccctgcggaa catgaacgag agatacggct acaagacaga ggacctggcc
     4861 ttcatccagg acaagcggtt caagttcgtg aaggacgcct acggcaagaa aaagtcccaa
     4921 gtgaataccg gattcttcct gagcctgcag gactacaacg gcgacacaca gaagaagctg
     4981 cacctgagcg gagtgggaat cgccctgctg atctgcctgt tcctggacaa gcagtacatc
     5041 aacatctttc tgagcaggct gcccatcttc tccagctaca atgcccagag cgaggaacgg
     5101 cggatcatca tcagatcctt cggcatcaac agcatcaagc tgcccaagga ccggatccac
     5161 agcgagaagt ccaacaagag cgtggccatg gatatgctca acgaagtgaa gcggtgcccc
     5221 gacgagctgt tcacaacact gtctgccgag aagcagtccc ggttcagaat catcagcgac
     5281 gaccacaatg aagtgctgat gaagcggagc agcgacagat tcgtgcctct gctgctgcag
     5341 tatatcgatt acggcaagct gttcgaccac atcaggttcc acgtgaacat gggcaagctg
     5401 agatacctgc tgaaggccga caagacctgc atcgacggcc agaccagagt cagagtgatc
     5461 gagcagcccc tgaacggctt cggcagactg gaagaggccg agacaatgcg gaagcaagag
     5521 aacggcacct tcggcaacag cggcatccgg atcagagact tcgagaacat gaagcgggac
     5581 gacgccaatc ctgccaacta tccctacatc gtggacacct acacacacta catcctggaa
     5641 aacaacaagg tcgagatgtt tatcaacgac aaagaggaca gcgccccact gctgcccgtg
     5701 atcgaggatg atagatacgt ggtcaagaca atccccagct gccggatgag caccctggaa
     5761 attccagcca tggccttcca catgtttctg ttcggcagca agaaaaccga gaagctgatc
     5821 gtggacgtgc acaaccggta caagagactg ttccaggcca tgcagaaaga agaagtgacc
     5881 gccgagaata tcgccagctt cggaatcgcc gagagcgacc tgcctcagaa gatcctggat
     5941 ctgatcagcg gcaatgccca cggcaaggat gtggacgcct tcatcagact gaccgtggac
     6001 gacatgctga ccgacaccga gcggagaatc aagagattca aggacgaccg gaagtccatt
     6061 cggagcgccg acaacaagat gggaaagaga ggcttcaagc agatctccac aggcaagctg
     6121 gccgacttcc tggccaagga catcgtgctg tttcagccca gcgtgaacga tggcgagaac
     6181 aagatcaccg gcctgaacta ccggatcatg cagagcgcca ttgccgtgta cgatagcggc
     6241 gacgattacg aggccaagca gcagttcaag ctgatgttcg agaaggcccg gctgatcggc
     6301 aagggcacaa cagagcctca tccatttctg tacaaggtgt tcgcccgcag catccccgcc
     6361 aatgccgtcg agttctacga gcgctacctg atcgagcgga agttctacct gaccggcctg
     6421 tccaacgaga tcaagaaagg caacagagtg gatgtgccct tcatccggcg ggaccagaac
     6481 aagtggaaaa cacccgccat gaagaccctg ggcagaatct acagcgagga tctgcccgtg
     6541 gaactgccca gacagatgtt cgacaatgag atcaagtccc acctgaagtc cctgccacag
     6601 atggaaggca tcgacttcaa caatgccaac gtgacctatc tgatcgccga gtacatgaag
     6661 agagtgctgg acgacgactt ccagaccttc taccagtgga accgcaacta ccggtacatg
     6721 gacatgctta agggcgagta cgacagaaag ggctccctgc agcactgctt caccagcgtg
     6781 gaagagagag aaggcctctg gaaagagcgg gcctccagaa cagagcggta cagaaagcag
     6841 gccagcaaca agatccgcag caaccggcag atgagaaacg ccagcagcga agagatcgag
     6901 acaatcctgg ataagcggct gagcaacagc cggaacgagt accagaaaag cgagaaagtg
     6961 atccggcgct acagagtgca ggatgccctg ctgtttctgc tggccaaaaa gaccctgacc
     7021 gaactggccg atttcgacgg cgagaggttc aaactgaaag aaatcatgcc cgacgccgag
     7081 aagggaatcc tgagcgagat catgcccatg agcttcacct tcgagaaagg cggcaagaag
     7141 tacaccatca ccagcgaggg catgaagctg aagaactacg gcgacttctt tgtgctggct
     7201 agcgacaaga ggatcggcaa cctgctggaa ctcgtgggca gcgacatcgt gtccaaagag
     7261 gatatcatgg aagagttcaa caaatacgac cagtgcaggc ccgagatcag ctccatcgtg
     7321 ttcaacctgg aaaagtgggc cttcgacaca taccccgagc tgtctgccag agtggaccgg
     7381 gaagagaagg tggacttcaa gagcatcctg aaaatcctgc tgaacaacaa gaacatcaac
     7441 aaagagcaga gcgacatcct gcggaagatc cggaacgcct tcgatcacaa caattacccc
     7501 gacaaaggcg tggtggaaat caaggccctg cctgagatcg ccatgagcat caagaaggcc
     7561 tttggggagt acgccatcat gaagTAAggC Cgcactcgag gcccgaaagg aagctgagtt
     7621 ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt
     7681 gaggggtttt ttgctgaaag gaggaactat atccggatat cccgcaagag gcccggcagt
     7741 accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg
     7801 agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa
     7861 ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaaacacc atcgaatggc
     7921 gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga gagtcaattc agggtggtga
     7981 atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg
     8041 tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag
     8101 cggcgatggc ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac
     8161 agtcgttgct gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg
     8221 tcgcggcgat taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag
     8281 aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca
     8341 gtgggctgat cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct
     8401 gcactaatgt tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta
     8461 ttttctccca tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc
     8521 agcaaatcgc gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg
     8581 gctggcataa atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact
     8641 ggagtgccat gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca
     8701 ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt
     8761 ccgggctgcg cgttggtgcg gatatctcgg tagtgggata cgacgatacc gaagacagct
     8821 catgttatat cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca
     8881 gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc
     8941 ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc
     9001 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     9061 agtgagcgca acgcaattaa tgtaagttag ctcactcatt aggcaccggg atctcgaccg
     9121 atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc
     9181 gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg
     9241 ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg
     9301 cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcactgg tcccgcc
//