LOCUS       pR0199_EiCsm6-RP1181_Hi 7377 bp ds-DNA     circular     30-AUG-2017
DEFINITION  .
SOURCE      synthetic DNA construct                                            
  ORGANISM  recombinant plasmid                                                
FEATURES             Location/Qualifiers
     misc_feature    2407..3699
                     /label="EiCsm6-RP1181"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    536..840
                     /label="GibsonInsert2"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1961..2109
                     /label="6x His tag/ Twin Strep"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2372..3733
                     /label="Gibsonpiece1"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             complement(495..1154)
                     /label="AmpR"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2110..2401
                     /label="Sumo"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_binding    1889..1911
                     /label="LacO"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer_bind     1870..1889
                     /label="T7"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    633..633
                     /label="replaced to remove BsaI site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     rep_origin      complement(join(7092..7377,1..397))
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
ORIGIN
        1 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
       61 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
      121 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
      181 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
      241 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
      301 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
      361 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
      421 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
      481 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
      541 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
      601 ccatctggcc ccagtgctgc aatgataccg cgtgacccac gctcaccggc tccagattta
      661 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
      721 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
      781 agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt
      841 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
      901 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
      961 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     1021 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     1081 cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact
     1141 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     1201 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     1261 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     1321 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     1381 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     1441 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     1501 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaagaa
     1561 ccgggatctc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg
     1621 ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac
     1681 agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg
     1741 aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca
     1801 cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc
     1861 ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag
     1921 aaataatttt gtttaacttt aagaaggaga tataccatgg gcagcagcca tcatcatcat
     1981 catcacagca gcggcctggt gccgcgcggc agccatatgg ctagctggag ccatccgcag
     2041 tttgaaaaag gtggtggtag cggtggtggt tcaggtggta gtgcatggtc acaccctcag
     2101 tttgagaaaa tgtcggactc agaagtcaat caagaagcta agccagaggt caagccagaa
     2161 gtcaagcctg agactcacat caatttaaag gtgtccgatg gatcttcaga gatcttcttc
     2221 aagatcaaaa agaccactcc tttaagaagg ctgatggaag cgttcgctaa aagacagggt
     2281 aaggaaatgg actccttaag attcttgtac gacggtatta gaatccaagc tgatcagacc
     2341 cctgaagatt tggacatgga ggataacgat attattgagg ctcacagaga acagattggt
     2401 gGATCTATGA AGATTCTTTT TAGCCCGATT GGCAACACAG ATCCTTGGCG CAATGACCGC
     2461 GATGGTGCGA TGTTGCACAT CGTTCGTCAC TACCAACCTG ACCGTGTAGT GCTTTTTTTT
     2521 ACCGAGTCCA TCTGGCAAGG GAATCAACAC TTCTCAGGAC AACAAGCCTT TGATTGGGTG
     2581 AAAATCATTC AGAGTATTAA CGAGAACTGT CAGATTGAAA TTAAATGTGA TACTATTGAG
     2641 GTGGAGAATG ATTTCGATGC CTACAAAGAT TTGTTCCATC AGTACTTAGT CGAGGAAAAA
     2701 CGTAAATATC CTAATGCTGA AATTTTCCTG AATGTCACAA GCGGGACTCC GCAGATGGAA
     2761 ACAACTTTGT GCCTTGAATA TGTGACTTAC CCCGACAAAA TGCGCTGCAT TCAGGTCAGC
     2821 ACCCCGCTTA AGACTAGCAA TGCTAAAACC AAATACGCGC AAGCCGATTG CCAGGAAGTT
     2881 GACCTTGAAA TCGTTAATGA AGAGGAGTCT CAGCAACCTT CACGCTGTCA CAAGATCGCT
     2941 ATTCTGAGCT TTCGTGAAGC AATCGTACGT AATCAGATCA AATCATTACT TGATAATTAC
     3001 GACTACGAAG CCGCGCTGCA GCTTGTTGCA TCACAAAAGA GTTTCCGCAA TGGGAAGGAG
     3061 ATTCGCAAAA AGCTGAAAGA GCTGATCGAC GACATTAAGA TGCACCGTGT GTTCTCATAC
     3121 TTAATCAAAC AGTATCCCCG CAACGAGAAG TTGCAAAAAG CTCTGTTACA CACCATTCTT
     3181 TTAGAAATGC GTCACCAACG TGGAGACATT GCCGAAACTC TGATCCGTGT AAAGAGTATT
     3241 GCAGAGTATA TCGTGGAACA ATATATCCAA AAGAACTATC CATATCTGAT CATCTACAAA
     3301 GAAGACAAGC CTTACTTCAA CGTTAGCTAC AGTCAAGAGC TGACCGAGAG TTATTTGGCC
     3361 CTGATGGACA GCCGCAACAA GAAGACTAAC AAGAAAATGA CCGTGGACTC CCTGGATCGT
     3421 ATCTTGGGGT TTCCCGCTTA CCGCGATTTC TTGCAACTGT TAGAAGCCAG CAACGAGATG
     3481 ACTAACGAGA TGAATAAAGT AAATGAAATT AATAACCTTC GCAATAAGGT CGCCCATAAC
     3541 TTAGACAGTC TTAATTTAGA TCGTGACAAG AATGGTCGCA AAATCACGAA TGCGGTAACT
     3601 GCGGTGCGCA CCATGCTGTT GGCGGTCTTC CCAGAGGTTC AGGAAAACGA CTTCCATTAT
     3661 TTAAAGCAAT TTAATCAGAG CATTAAAGAA CTTTTGTAAg gCCgcactcg aggcccgaaa
     3721 ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc
     3781 taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atcccgcaag
     3841 aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg
     3901 aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt
     3961 aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaaaca
     4021 ccatcgaatg gcgcaaaacc tttcgcggta tggcatgata gcgcccggaa gagagtcaat
     4081 tcagggtggt gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat gccggtgtct
     4141 cttatcagac cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg
     4201 aaaaagtgga agcggcgatg gcggagctga attacattcc caaccgcgtg gcacaacaac
     4261 tggcgggcaa acagtcgttg ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc
     4321 cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca actgggtgcc agcgtggtgg
     4381 tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg
     4441 cgcaacgcgt cagtgggctg atcattaact atccgctgga tgaccaggat gccattgctg
     4501 tggaagctgc ctgcactaat gttccggcgt tatttcttga tgtctctgac cagacaccca
     4561 tcaacagtat tattttctcc catgaagacg gtacgcgact gggcgtggag catctggtcg
     4621 cattgggtca ccagcaaatc gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc
     4681 tgcgtctggc tggctggcat aaatatctca ctcgcaatca aattcagccg atagcggaac
     4741 gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg
     4801 gcatcgttcc cactgcgatg ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg
     4861 ccattaccga gtccgggctg cgcgttggtg cggatatctc ggtagtggga tacgacgata
     4921 ccgaagacag ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc
     4981 tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca
     5041 atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa
     5101 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     5161 tggaaagcgg gcagtgagcg caacgcaatt aatgtaagtt agctcactca ttaggcaccg
     5221 ggatctcgac cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg
     5281 ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag
     5341 gtgccggcag cgctctgggt cattttcggc gaggaccgct ttcgctggag cgcgacgatg
     5401 atcggcctgt cgcttgcggt attcggaatc ttgcacgccc tcgctcaagc cttcgtcact
     5461 ggtcccgcca ccaaacgttt cggcgagaag caggccatta tcgccggcat ggcggccgac
     5521 gcgctgggct acgtcttgct ggcgttcgcg acgcgaggct ggatggcctt ccccattatg
     5581 attcttctcg cttccggcgg catcgggatg cccgcgttgc aggccatgct gtccaggcag
     5641 gtagatgacg accatcaggg acagcttcaa ggatcgctcg cggctcttac cagcctaact
     5701 tcgatcactg gaccgctgat cgtcacggcg atttatgccg cctcggcgag cacatggaac
     5761 gggttggcat ggattgtagg cgccgcccta taccttgtct gcctccccgc gttgcgtcgc
     5821 ggtgcatgga gccgggccac ctcgacctga atggaagccg gcggcacctc gctaacggat
     5881 tcaccactcc aagaattgga gccaatcaat tcttgcggag aactgtgaat gcgcaaacca
     5941 acccttggca gaacatatcc atcgcgtccg ccatctccag cagccgcacg cggcgcatct
     6001 cgggcagcgt tgggtcctgg ccacgggtgc gcatgatcgt gctcctgtcg ttgaggaccc
     6061 ggctaggctg gcggggttgc cttactggtt agcagaatga atcaccgata cgcgagcgaa
     6121 cgtgaagcga ctgctgctgc aaaacgtctg cgacctgagc aacaacatga atggtcttcg
     6181 gtttccgtgt ttcgtaaagt ctggaaacgc ggaagtcagc gccctgcacc attatgttcc
     6241 ggatctgcat cgcaggatgc tgctggctac cctgtggaac acctacatct gtattaacga
     6301 agcgctggca ttgaccctga gtgatttttc tctggtcccg ccgcatccat accgccagtt
     6361 gtttaccctc acaacgttcc agtaaccggg catgttcatc atcagtaacc cgtatcgtga
     6421 gcatcctctc tcgtttcatc ggtatcatta cccccatgaa cagaaatccc ccttacacgg
     6481 aggcatcagt gaccaaacag gaaaaaaccg cccttaacat ggcccgcttt atcagaagcc
     6541 agacattaac gcttctggag aaactcaacg agctggacgc ggatgaacag gcagacatct
     6601 gtgaatcgct tcacgaccac gctgatgagc tttaccgcag ctgcctcgcg cgtttcggtg
     6661 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     6721 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     6781 gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc
     6841 atcagagcag attgtactga gagtgcacca tatatgcggt gtgaaatacc gcacagatgc
     6901 gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc
     6961 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     7021 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     7081 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     7141 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     7201 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     7261 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     7321 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga acccccc
//