LOCUS       pDestTol2pAacry-        7092 bp    DNA     circular UNA 07-AUG-2018
DEFINITION  an expression clone created from pDestTol2pACryGFP and Reaction of
            3 fragments.
ACCESSION   urn.local...b8-9craxq9
VERSION     urn.local...b8-9craxq9
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    2714..2734
                     /note="Geneious type: attB4"
                     /standard_name="attB4"
     misc_recomb     <2725..2734
                     /standard_name="attL4"
     misc_recomb     <2725..2734
                     /standard_name="attB4"
     misc_feature    <2725..2727
                     /standard_name="Gateway Recomb4"
     misc_feature    complement(2736..2741)
                     /standard_name="Kozak(3)"
     misc_recomb     2932..>2942
                     /standard_name="AttR1"
     misc_recomb     2932..>2942
                     /standard_name="attB1"
     misc_feature    2932..2957
                     /note="Geneious type: attP1"
                     /standard_name="attP1"
     misc_feature    2939..>2942
                     /standard_name="Gateway recomb1"
     misc_feature    2958..2960
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     misc_feature    2965..2968
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     misc_feature    2983..3042
                     /note="Geneious type: motif"
                     /standard_name="mem sequence"
     primer_bind     2983..3005
                     /standard_name="679 F"
     primer_bind     2983..3004
                     /standard_name="679 F"
     primer_bind     2983..3003
                     /standard_name="679 F"
     gene            3043..3756
                     /standard_name="Killer Red"
     misc_feature    complement(3736..3756)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="1,452 R"
     misc_feature    complement(3737..3756)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="1,452 R"
     misc_feature    3760..3764
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     misc_feature    3759^3760
                     /note="Geneious type: Editing History Deletion"
                     /standard_name="GGCC"
     misc_feature    complement(3765..3789)
                     /note="Geneious type: attB2"
                     /standard_name="attB2"
     polyA_signal    3790..3987
     misc_feature    complement(3988..4009)
                     /note="Geneious type: attB3"
                     /standard_name="attB3"
ORIGIN      
        1 nwtasmdstn cccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
       61 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
      121 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
      181 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccacg
      241 ctggctaaga actcatcagc ctccccggtc catctaccca cgtaccaatg caccaattgg
      301 ccacaatgac ggctactaca tggtgccatt ccttcctctt tataggaatg gagactacct
      361 cctgtccaac aaggctcttg gatacgagta cgcctacctg ttggacccag gtcattgcac
      421 aacaccagaa atgccctctg atctgcaaaa gacgtgaata tctgttcaga cacccatatc
      481 cactctgttc cacacaggtc agaggtttgt ccaggagttc ttgacagagg tgtaaaaagt
      541 actcaaaaat tttactcaag tgaaagtaca agtacttagg gaaaatttta ctcaattaaa
      601 agtaaaagta tctggctaga atcttacttg agtaaaagta aaaaagtact ccattaaaat
      661 tgtacttgag tattaaggaa gtaaaagtaa aagcaagaaa gaaaactaga gattcttgtt
      721 taagctttta atctcaaaaa acattaaatg aaatgcatac aaggttttat cctgctttag
      781 aactgtttgt atttaattat caaactataa gacagacaat ctaatgccag tacacgctac
      841 tcaaagttgt aaaacctcag atttaacttc agtagaagct gattctcaaa attgttagtg
      901 tcaagcctag ctcttttggg gctgaaaagc aatcctgcag tgctgaaaag cctctcacag
      961 gcagccgatg cgggaagagg tgtattagtc ttgatagaga ggctgcaaat agcaggaaac
     1021 gtgagcagag actccctggt gtctgaaaca caggccagat tggccttaag atacattgat
     1081 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     1141 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     1201 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa
     1261 aacctctaca aatgtggtat ggctgattat gatctagagt cgcggccgct ttacttgtac
     1321 agctcgtcca tgccgagagt gatcccggcg gcggtcacga actccagcag gaccatgtga
     1381 tcgcgcttct cgttggggtc tttgctcagg gcggactggg tgctcaggta gtggttgtcg
     1441 ggcagcagca cggggccgtc gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc
     1501 acgctgccgt cctcgatgtt gtggcggatc ttgaagttca ccttgatgcc gttcttctgc
     1561 ttgtcggcca tgatatagac gttgtggctg ttgtagttgt actccagctt gtgccccagg
     1621 atgttgccgt cctccttgaa gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg
     1681 ccctcgaact tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg
     1741 cgctcctgga cgtagccttc gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg
     1801 tcggggtagc ggctgaagca ctgcacgccg taggtcaggg tggtcacgag ggtgggccag
     1861 ggcacgggca gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca
     1921 tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg
     1981 accaggatgg gcaccacccc ggtgaacagc tcctcgccct tgctcaccat ggtggcgacc
     2041 ggtaatgtca gacctggtaa ctccttactg taacttattg cacctgatga acaaaagatg
     2101 tgaactgaac gcaagtagga ggctaataga ggctagtaca gtacaaagat ccccaaatga
     2161 tgtgtgccgg ccttagtgga ggcagacccc tggttatata cctgtgctgc agaaagaggg
     2221 gcttacaaat ggatctctct ggaatggcat tatctcatgg tgggaacagt gttgtcagca
     2281 gaatcagcgg ggtggactct gacctgagaa tgacagtcta gcactgaatg gctcagacaa
     2341 tactcttgtg gccaactatc accggaaaat aagtgcctgc caaaacaaac cagaccagat
     2401 gaagctacat ggctgtagaa cgtcagtatt taatgtgttg tggccacttg gttcaccaga
     2461 tttaagagct aattttttta aatatcacag ataatcaatc gctttttttt ttcttgactg
     2521 aaaagcttag actggaaaat tctttgaaaa agtttgcact acagaaaata aatacataac
     2581 atcctgcact gaatgcacac tattaataga aaagagagca ttattgtagg aaatgtattg
     2641 tatatgtaca ttctgtgcat ttcacaattt tctcgagcag gaaacagcta tgaccatgat
     2701 tacgccaagc tatcaacttt gtatagaaaa gttgcggtgg cttctaatcc gtgagtccta
     2761 gcgggtgaca gccctccgtc ttcacaggcg gaggagagtc ttccgtaggg ttcctcggag
     2821 tactgtcctc cgacgcgtgc aagggtcgac tctagagggt atataatgga tcccatcgcg
     2881 tctcagcctc actttgagct cctccacacg aattccctcg acctcgaaat tcaagtttgt
     2941 acaaaaaagc aggcttacgg gatcgatcca ccggtcgcca ccatgctgtg ctgtatgaga
     3001 agaaccaaac aggttgaaaa gaatgatgag gaccaaaaga tctccgaggg cggccccgcc
     3061 ctgttccaga gcgacatgac cttcaaaatc ttcatcgacg gcgaggtgaa cggccagaag
     3121 ttcaccatcg tggccgacgg cagcagcaag ttcccccacg gcgacttcaa cgtgcacgcc
     3181 gtgtgcgaga ccggcaagct gcccatgagc tggaagccca tctgccacct gatccagtac
     3241 ggcgagccct tcttcgcccg ctaccccgac ggcatcagcc atttcgccca ggagtgcttc
     3301 cccgagggcc tgagcatcga ccgcaccgtg cgcttcgaga acgacggcac catgaccagc
     3361 caccacacct acgagctgga cgacacctgc gtggtgagcc gcatcaccgt gaactgcgac
     3421 ggcttccagc ccgacggccc catcatgcgc gaccagctgg tggacatcct gcccaacgag
     3481 acccacatgt tcccccacgg ccccaacgcc gtgcgccagc tggccttcat cggcttcacc
     3541 accgccgacg gcggcctgat gatgggccac ttcgacagca agatgacctt caacggcagc
     3601 cgcgccatcg agatccccgg cccacacttc gtgaccatca tcaccaagca gatgagggac
     3661 accagcgaca agcgcgacca cgtgtgccag cgcgaggtgg cctacgccca cagcgtgccc
     3721 cgcatcacca gcgccatcgg tagcgacgag gattaaagca attctaccca gctttcttgt
     3781 acaaagtggg ggatccagac atgataagat acattgatga gtttggacaa accacaacta
     3841 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     3901 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     3961 ttcaggggga ggtgtgggag gttttttcca actttattat acatagttga taattcactg
     4021 gccgtcgttt tacggtacca tcgatgatga tccagacatg ataagataca ttgatgagtt
     4081 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     4141 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
     4201 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct
     4261 ctacaaatgt ggtatggctg attatgatcc tctagatcag atctgcgaag atacggccac
     4321 gggtgctctt gatcctgtgg ctgattttgg actgtgctgc tcgcagctgc tgatgaatca
     4381 catacttcct ccattttctt ccactgattg actgttataa tttccctaat ttccaggtca
     4441 aggtgctgtg cattgtggta atagatgtga catgacgtca cttccaaagg accaatgaac
     4501 atgtctgacc aatttcatat aatgtgaaaa cgattttcat aggcagaata aataacattt
     4561 aaattaaact gggcatcagc gcaattcaat tggtttggta atagcaaggg aaaatagaat
     4621 gaagtgatct ccaaaaaata agtacttttt gactgtaaat aaaattgtaa ggagtaaaaa
     4681 gtactttttt ttctaaaaaa atgtaattaa gtaaaagtaa aagtattgat ttttaattgt
     4741 actcaagtaa agtaaaaatc cccaaaaata atacttaagt acagtaatca agtaaaatta
     4801 ctcaagtact ttacacctct ggttcttgac cccctacctt cagcaagccc agcagatcca
     4861 ctagttctag agcggccgcc accgcggtgg agctccagct tttgttccct ttagtgaggg
     4921 ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     4981 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     5041 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     5101 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     5161 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     5221 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     5281 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     5341 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     5401 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     5461 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     5521 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     5581 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     5641 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     5701 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     5761 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     5821 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     5881 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     5941 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     6001 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     6061 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     6121 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     6181 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     6241 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     6301 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     6361 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     6421 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     6481 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     6541 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     6601 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     6661 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     6721 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     6781 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     6841 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     6901 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     6961 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     7021 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     7081 ccccgaaaag tg
//
