LOCUS       pDSG323                 5635 bp    DNA     circular SYN 14-JUN-2018
DEFINITION  Synthetic plasmid.
ACCESSION   pDSG323
VERSION
KEYWORDS    .
SOURCE      Synthetic plasmid
  ORGANISM  Synthetic plasmid
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 5635)
  AUTHORS   Glass,D.S. and Riedel-Kruse,I.H.
  TITLE     A synthetic bacterial cell-cell adhesion toolbox for programming
            multicellular morphologies and patterns
  JOURNAL   Cell (2018) In press
REFERENCE   2  (bases 1 to 5635)
  AUTHORS   Glass,D.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2018) Bioengineering, Stanford University, 318
            Campus Drive, Clark Center E350, Stanford, CA 94305, USA
COMMENT     Bankit Comment: ALT EMAIL:dsglass@gmail.com
            Bankit Comment: Vecscreen Comment:Submitter says that this sequence
            represents a Cloning Vector
            Bankit Comment: TOTAL # OF SEQS:83.
FEATURES             Location/Qualifiers
     source          1..5635
                     /organism="Synthetic plasmid"
                     /mol_type="other DNA"
                     /db_xref="taxon:1440148"
     misc_feature    1..689
                     /label=BBa_I732084
     misc_feature    1..36
                     /label=pLacIQ (BBa_I4032, 1nt deletion)
     misc_feature    45..56
                     /label=BBa_J04500(1)
     misc_feature    45..56
                     /label=RBS (BBa_B0034)
     CDS             63..686
                     /codon_start=1
                     /transl_table=11
                     /product="tetR ORF (BBa_I732080)"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     misc_feature    698..826
                     /label=Double terminator (BBa_B0015)
     misc_feature    835..896
                     /label=BBa_J13002
     misc_feature    835..888
                     /label=pTet (BBa_R0040)
     misc_feature    897..908
                     /label=BBa_J04500
     misc_feature    897..908
                     /label=RBS (BBa_B0034)(1)
     CDS             915..2882
                     /codon_start=1
                     /transl_table=11
                     /product="Neae_removedRestrictionsites"
                     /translation="MITHGCYTRTRHKHKLKKTLIMLSAGLGLFFYVNQNSFANGENY
                     FKLGSDSKLLTHDSYQNRLFYTLKTGETVADLSKSQDINLSTIWSLNKHLYSSESEMM
                     KAAPGQQIILPLKKLPFEYSALPLLGSAPLVAAGGVAGHTNKLTKMSPDVTKSNMTDD
                     KALNYAAQQAASLGSQLQSRSLNGDYAKDTALGIAGNQASSQLQAWLQHYGTAEVNLQ
                     SGNNFDGSSLDFLLPFYDSEKMLAFGQVGARYIDSRFTANLGAGQRFFLPANMLGYNV
                     FIDQDFSGDNTRLGIGGEYWRDYFKSSVNGYFRMSGWHESYNKKDYDERPANGFDIRF
                     NGYLPSYPALGAKLIYEQYYGDNVALFNSDKLQSNPGAATVGVNYTPIPLVTMGIDYR
                     HGTGNENDLLYSMQFRYQFDKSWSQQIEPQYVNELRTLSGSRYDLVQRNNNIILEYKK
                     QDILSLNIPHDINGTEHSTQKIQLIVKSKYGLDRIVWDDSALRSQGGQIQHSGSQSAQ
                     DYQAILPAYVQGGSNIYKVTARAYDRNGNSSNNVQLTITVLSNGQVVDQVGVTDFTAD
                     KTSAKADNADTITYTATVKKNGVAQANVPVSFNIVSGTATLGANSAKTDANGKATVTL
                     KSSTPGQVVVSAKTAEMTSALNASAVIFFDGA"
     misc_feature    2883..2908
                     /label=RFC[23] biobrick suffix (partial)
     misc_feature    2909..2980
                     /label=his operon terminator
     misc_feature    complement(3044..3061)
                     /label=VR primer site
     CDS             complement(3124..3939)
                     /codon_start=1
                     /transl_table=11
                     /product="KanR"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     misc_feature    complement(4271..5095)
                     /label=p15A ORI
     misc_feature    5496..5516
                     /label=VF2 primer site
     misc_feature    5614..5635
                     /label=biobrick prefix
BASE COUNT     1580 a   1258 c   1294 g   1503 t
ORIGIN      
        1 tggtgcaaaa cctttgcggt atggcatgat agcgcctact agagaaagag gagaaatact
       61 agatgtccag attagataaa agtaaagtga ttaacagcgc attagagctg cttaatgagg
      121 tcggaatcga aggtttaaca acccgtaaac tcgcccagaa gctaggtgta gagcagccta
      181 cattgtattg gcatgtaaaa aataagcggg ctttgctcga cgccttagcc attgagatgt
      241 tagataggca ccatactcac ttttgccctt tagaagggga aagctggcaa gattttttac
      301 gtaataacgc taaaagtttt agatgtgctt tactaagtca tcgcgatgga gcaaaagtac
      361 atttaggtac acggcctaca gaaaaacagt atgaaactct cgaaaatcaa ttagcctttt
      421 tatgccaaca aggtttttca ctagagaatg cattatatgc actcagcgct gtggggcatt
      481 ttactttagg ttgcgtattg gaagatcaag agcatcaagt cgctaaagaa gaaagggaaa
      541 cacctactac tgatagtatg ccgccattat tacgacaagc tatcgaatta tttgatcacc
      601 aaggtgcaga gccagccttc ttattcggcc ttgaattgat catatgcgga ttagaaaaac
      661 aacttaaatg tgaaagtggg tcctaataat actagagcca ggcatcaaat aaaacgaaag
      721 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
      781 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agagtcccta
      841 tcagtgatag agattgacat ccctatcagt gatagagata ctgagcacta ctagagaaag
      901 aggagaaata ctagatgatt actcatggtt gttatacccg gacccggcac aagcataagc
      961 taaaaaaaac attgattatg cttagtgctg gtttaggatt gtttttttat gttaatcaga
     1021 actcatttgc aaatggtgaa aattatttta aattgggttc ggattcaaaa ctgttaactc
     1081 atgatagcta tcagaatcgc cttttttata cgttgaaaac tggtgaaact gttgccgatc
     1141 tttctaaatc gcaagatatt aatttatcga cgatttggtc gttgaataag catttataca
     1201 gttctgaaag cgaaatgatg aaggccgcgc ctggtcagca gatcattttg ccactcaaaa
     1261 aacttccctt tgaatacagt gcactaccac ttttaggttc ggcacctctt gttgctgcgg
     1321 gtggtgttgc tggtcacacg aataaactga ctaaaatgtc cccggacgtg accaaaagca
     1381 acatgaccga tgacaaggca ttaaattatg cggcacaaca ggcggcgagt ctcggtagcc
     1441 agcttcagtc gcgatctctg aacggcgatt acgcgaaaga taccgctctt ggtatcgctg
     1501 gtaaccaggc ttcgtcacag ttgcaggcct ggttacaaca ttatggaacg gcagaggtta
     1561 atctgcaaag tggtaataac tttgacggta gttcactgga cttcttatta ccgttctatg
     1621 attccgaaaa aatgctggca tttggtcagg tcggagcgcg ttacattgac tcccgcttta
     1681 cggcaaattt aggtgcgggt cagcgttttt tccttcctgc aaacatgttg ggctataacg
     1741 tcttcattga tcaggatttt tctggtgata atacccgttt aggtattggt ggcgaatact
     1801 ggcgagacta tttcaaaagt agcgttaacg gctatttccg catgagcggc tggcatgagt
     1861 catacaataa gaaagactat gatgagcgcc cagcaaatgg cttcgatatc cgttttaatg
     1921 gctatctacc gtcatatccg gcattaggcg ccaagctgat atatgagcag tattatggtg
     1981 ataatgttgc tttgtttaat tctgataagc tgcaatcgaa tcctggtgcg gcgaccgttg
     2041 gtgtaaacta tactccgatt cctctggtga cgatggggat cgattaccgt catggtacgg
     2101 gtaatgaaaa tgatctcctt tactcaatgc agttccgtta tcagtttgat aaatcgtggt
     2161 ctcagcaaat tgaaccacag tatgttaacg agttaagaac attatcaggc agccgttacg
     2221 atctggttca gcgtaataac aatattattc tggagtacaa gaagcaggat attctttctc
     2281 tgaatattcc gcatgatatt aatggtactg aacacagtac gcagaagatt cagttgatcg
     2341 ttaagagcaa atacggtctg gatcgtatcg tctgggatga tagtgcatta cgcagtcagg
     2401 gcggtcagat tcagcatagc ggaagccaaa gcgcacaaga ctaccaggct attttgcctg
     2461 cttatgtgca aggtggcagc aatatttata aagtgacggc tcgcgcctat gaccgtaatg
     2521 gcaatagctc taacaatgta cagcttacta ttaccgttct gtcgaatggt caagttgtcg
     2581 accaggttgg ggtaacggac tttacggcgg ataagacttc ggctaaagcg gataacgccg
     2641 ataccattac ttataccgcg acggtgaaaa agaatggggt agctcaggct aatgtccctg
     2701 tttcatttaa tattgtttca ggaactgcaa ctcttggggc aaatagtgcc aaaacggatg
     2761 ctaacggtaa ggcaaccgta acgttgaagt cgagtacgcc aggacaggtc gtcgtgtctg
     2821 ctaaaaccgc ggagatgact tcagcactta atgccagtgc ggttatattt tttgatggtg
     2881 cgactagtta ataaagcggc cgctgcagtc cggcaaaaaa gggcaaggtg tcaccaccct
     2941 gccctttttc tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg
     3001 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     3061 tctcgagtcc cgtcaagtca gcgtaatgct ctgccagtgt tacaaccaat taaccaattc
     3121 tgattagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc
     3181 aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt
     3241 ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca
     3301 acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac
     3361 gactgaatcc ggtgagaatg gcaaaagctt atgcatttct ttccagactt gttcaacagg
     3421 ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga
     3481 ttgcgcctga gcgagacgaa atacgcgatc gctgttaaaa ggacaattac aaacaggaat
     3541 cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg
     3601 atattcttct aatacctgga atgctgtttt cccggggatc gcagtggtga gtaaccatgc
     3661 atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca
     3721 gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag
     3781 aaacaactct ggcgcatcgg gcttcccata caatcgatag attgtcgcac ctgattgccc
     3841 gacattatcg cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg
     3901 cggcctcgag caagacgttt cccgttgaat atggctcata acaccccttg tattactgtt
     3961 tatgtaagca gacagtttta ttgttcatga tgatatattt ttatcttgtg caatgtaaca
     4021 tcagagattt tgagacacaa cgtggctttg ttgaataaat cgaacttttg ctgagttgaa
     4081 ggatcagatc acgcatcttc ccgacaacgc agaccgttcc gtggcaaagc aaaagttcaa
     4141 aatcaccaac tggtccacct acaacaaagc tctcatcaac cgtggctccc tcactttctg
     4201 gctggatgat ggggcgattc aggcctggta tgagtcagca acaccttctt cacgaggcag
     4261 acctcagcgc tagcggagtg tatactggct tactatgttg gcactgatga gggtgtcagt
     4321 gaagtgcttc atgtggcagg agaaaaaagg ctgcaccggt gcgtcagcag aatatgtgat
     4381 acaggatata ttccgcttcc tcgctcactg actcgctacg ctcggtcgtt cgactgcggc
     4441 gagcggaaat ggcttacgaa cggggcggag atttcctgga agatgccagg aagatactta
     4501 acagggaagt gagagggccg cggcaaagcc gtttttccat aggctccgcc cccctgacaa
     4561 gcatcacgaa atctgacgct caaatcagtg gtggcgaaac ccgacaggac tataaagata
     4621 ccaggcgttt cccctggcgg ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc
     4681 ggtgtcattc cgctgttatg gccgcgtttg tctcattcca cgcctgacac tcagttccgg
     4741 gtaggcagtt cgctccaagc tggactgtat gcacgaaccc cccgttcagt ccgaccgctg
     4801 cgccttatcc ggtaactatc gtcttgagtc caacccggaa agacatgcaa aagcaccact
     4861 ggcagcagcc actggtaatt gatttagagg agttagtctt gaagtcatgc gccggttaag
     4921 gctaaactga aaggacaagt tttggtgact gcgctcctcc aagccagtta cctcggttca
     4981 aagagttggt agctcagaga accttcgaaa aaccgccctg caaggcggtt ttttcgtttt
     5041 cagagcaaga gattacgcgc agaccaaaac gatctcaaga agatcatctt attaaggggt
     5101 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     5161 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     5221 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     5281 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     5341 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     5401 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     5461 caactttatc cgcctccatc cagtctattc catggtgcca cctgacgtct aagaaaccat
     5521 tattatcatg acattaacct ataaaaatag gcgtatcacg aggcagaatt tcagataaaa
     5581 aaaatcctta gctttcgcta aggatgattt ctggaattcg cggccgcttc tagag
//
LOCUS       pDSG290                 6701 bp    DNA     circular SYN 14-JUN-2018
DEFINITION  Synthetic plasmid.
ACCESSION   pDSG290
VERSION
KEYWORDS    .
SOURCE      Synthetic plasmid
  ORGANISM  Synthetic plasmid
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 6701)
  AUTHORS   Glass,D.S. and Riedel-Kruse,I.H.
  TITLE     A synthetic bacterial cell-cell adhesion toolbox for programming
            multicellular morphologies and patterns
  JOURNAL   Cell (2018) In press
REFERENCE   2  (bases 1 to 6701)
  AUTHORS   Glass,D.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2018) Bioengineering, Stanford University, 318
            Campus Drive, Clark Center E350, Stanford, CA 94305, USA
COMMENT     Bankit Comment: ALT EMAIL:dsglass@gmail.com
            Bankit Comment: Vecscreen Comment:Submitter says that this sequence
            represents a Cloning Vector
            Bankit Comment: TOTAL # OF SEQS:83.
FEATURES             Location/Qualifiers
     source          1..6701
                     /organism="Synthetic plasmid"
                     /mol_type="other DNA"
                     /db_xref="taxon:1440148"
     misc_feature    1..689
                     /label=BBa_I732084
     misc_feature    1..36
                     /label=pLacIQ (BBa_I4032, 1nt deletion)
     misc_feature    45..56
                     /label=BBa_J04500(1)
     misc_feature    45..56
                     /label=RBS (BBa_B0034)(1)
     CDS             63..686
                     /codon_start=1
                     /transl_table=11
                     /product="tetR ORF (BBa_I732080)"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     misc_feature    698..826
                     /label=Double terminator (BBa_B0015)
     misc_feature    835..896
                     /label=BBa_J13002
     misc_feature    835..888
                     /label=pTet (BBa_R0040)
     misc_feature    897..908
                     /label=BBa_J04500
     misc_feature    897..908
                     /label=RBS (BBa_B0034)
     CDS             915..2882
                     /codon_start=1
                     /transl_table=11
                     /product="Neae_removedRestrictionsites"
                     /translation="MITHGCYTRTRHKHKLKKTLIMLSAGLGLFFYVNQNSFANGENY
                     FKLGSDSKLLTHDSYQNRLFYTLKTGETVADLSKSQDINLSTIWSLNKHLYSSESEMM
                     KAAPGQQIILPLKKLPFEYSALPLLGSAPLVAAGGVAGHTNKLTKMSPDVTKSNMTDD
                     KALNYAAQQAASLGSQLQSRSLNGDYAKDTALGIAGNQASSQLQAWLQHYGTAEVNLQ
                     SGNNFDGSSLDFLLPFYDSEKMLAFGQVGARYIDSRFTANLGAGQRFFLPANMLGYNV
                     FIDQDFSGDNTRLGIGGEYWRDYFKSSVNGYFRMSGWHESYNKKDYDERPANGFDIRF
                     NGYLPSYPALGAKLIYEQYYGDNVALFNSDKLQSNPGAATVGVNYTPIPLVTMGIDYR
                     HGTGNENDLLYSMQFRYQFDKSWSQQIEPQYVNELRTLSGSRYDLVQRNNNIILEYKK
                     QDILSLNIPHDINGTEHSTQKIQLIVKSKYGLDRIVWDDSALRSQGGQIQHSGSQSAQ
                     DYQAILPAYVQGGSNIYKVTARAYDRNGNSSNNVQLTITVLSNGQVVDQVGVTDFTAD
                     KTSAKADNADTITYTATVKKNGVAQANVPVSFNIVSGTATLGANSAKTDANGKATVTL
                     KSSTPGQVVVSAKTAEMTSALNASAVIFFDGA"
     CDS             2889..3221
                     /codon_start=1
                     /transl_table=11
                     /product="Nanobody R4P43 against human P53 transactivation
                     do"
                     /translation="-
                     VQLQESGGGSVRPGESLRLSCIAAGTLFSGKAVGWYRQAPGKDRELVAALSSGRETYY
                     IGPVKGRFIISRDDNKDTLYLQMNNLKLEDTAIYTCRLGLHWGQGTQVTVSS"
     misc_feature    3222..3227
                     /label=Stop codons
     regulatory      3236..3364
                     /regulatory_class="terminator"
                     /label=terminator B0015
     misc_feature    3365..3385
                     /label=biobrick suffix
     misc_feature    3386..3457
                     /label=his operon terminator
     misc_feature    complement(3521..3538)
                     /label=VR primer site
     CDS             complement(3746..4696)
                     /codon_start=1
                     /transl_table=11
                     /product="repA"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRK
                     ERTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAK
                     FSSEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHK
                     ANIEISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTL
                     IFQVELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTS
                     FEAKFLSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     misc_feature    complement(4700..4712)
                     /label=RBS for repA
     regulatory      complement(4717..4736)
                     /regulatory_class="promoter"
                     /label=repA promoter
     CDS             complement(5582..6442)
                     /codon_start=1
                     /transl_table=11
                     /product="AmpR"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     misc_feature    complement(6443..6521)
                     /label=Promoter/RBS for AmpR
     misc_feature    6562..6582
                     /label=VF2 primer site
     misc_feature    6680..6701
                     /label=BioBricks prefix
BASE COUNT     1817 a   1432 c   1495 g   1957 t
ORIGIN      
        1 tggtgcaaaa cctttgcggt atggcatgat agcgcctact agagaaagag gagaaatact
       61 agatgtccag attagataaa agtaaagtga ttaacagcgc attagagctg cttaatgagg
      121 tcggaatcga aggtttaaca acccgtaaac tcgcccagaa gctaggtgta gagcagccta
      181 cattgtattg gcatgtaaaa aataagcggg ctttgctcga cgccttagcc attgagatgt
      241 tagataggca ccatactcac ttttgccctt tagaagggga aagctggcaa gattttttac
      301 gtaataacgc taaaagtttt agatgtgctt tactaagtca tcgcgatgga gcaaaagtac
      361 atttaggtac acggcctaca gaaaaacagt atgaaactct cgaaaatcaa ttagcctttt
      421 tatgccaaca aggtttttca ctagagaatg cattatatgc actcagcgct gtggggcatt
      481 ttactttagg ttgcgtattg gaagatcaag agcatcaagt cgctaaagaa gaaagggaaa
      541 cacctactac tgatagtatg ccgccattat tacgacaagc tatcgaatta tttgatcacc
      601 aaggtgcaga gccagccttc ttattcggcc ttgaattgat catatgcgga ttagaaaaac
      661 aacttaaatg tgaaagtggg tcctaataat actagagcca ggcatcaaat aaaacgaaag
      721 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
      781 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agagtcccta
      841 tcagtgatag agattgacat ccctatcagt gatagagata ctgagcacta ctagagaaag
      901 aggagaaata ctagatgatt actcatggtt gttatacccg gacccggcac aagcataagc
      961 taaaaaaaac attgattatg cttagtgctg gtttaggatt gtttttttat gttaatcaga
     1021 actcatttgc aaatggtgaa aattatttta aattgggttc ggattcaaaa ctgttaactc
     1081 atgatagcta tcagaatcgc cttttttata cgttgaaaac tggtgaaact gttgccgatc
     1141 tttctaaatc gcaagatatt aatttatcga cgatttggtc gttgaataag catttataca
     1201 gttctgaaag cgaaatgatg aaggccgcgc ctggtcagca gatcattttg ccactcaaaa
     1261 aacttccctt tgaatacagt gcactaccac ttttaggttc ggcacctctt gttgctgcgg
     1321 gtggtgttgc tggtcacacg aataaactga ctaaaatgtc cccggacgtg accaaaagca
     1381 acatgaccga tgacaaggca ttaaattatg cggcacaaca ggcggcgagt ctcggtagcc
     1441 agcttcagtc gcgatctctg aacggcgatt acgcgaaaga taccgctctt ggtatcgctg
     1501 gtaaccaggc ttcgtcacag ttgcaggcct ggttacaaca ttatggaacg gcagaggtta
     1561 atctgcaaag tggtaataac tttgacggta gttcactgga cttcttatta ccgttctatg
     1621 attccgaaaa aatgctggca tttggtcagg tcggagcgcg ttacattgac tcccgcttta
     1681 cggcaaattt aggtgcgggt cagcgttttt tccttcctgc aaacatgttg ggctataacg
     1741 tcttcattga tcaggatttt tctggtgata atacccgttt aggtattggt ggcgaatact
     1801 ggcgagacta tttcaaaagt agcgttaacg gctatttccg catgagcggc tggcatgagt
     1861 catacaataa gaaagactat gatgagcgcc cagcaaatgg cttcgatatc cgttttaatg
     1921 gctatctacc gtcatatccg gcattaggcg ccaagctgat atatgagcag tattatggtg
     1981 ataatgttgc tttgtttaat tctgataagc tgcaatcgaa tcctggtgcg gcgaccgttg
     2041 gtgtaaacta tactccgatt cctctggtga cgatggggat cgattaccgt catggtacgg
     2101 gtaatgaaaa tgatctcctt tactcaatgc agttccgtta tcagtttgat aaatcgtggt
     2161 ctcagcaaat tgaaccacag tatgttaacg agttaagaac attatcaggc agccgttacg
     2221 atctggttca gcgtaataac aatattattc tggagtacaa gaagcaggat attctttctc
     2281 tgaatattcc gcatgatatt aatggtactg aacacagtac gcagaagatt cagttgatcg
     2341 ttaagagcaa atacggtctg gatcgtatcg tctgggatga tagtgcatta cgcagtcagg
     2401 gcggtcagat tcagcatagc ggaagccaaa gcgcacaaga ctaccaggct attttgcctg
     2461 cttatgtgca aggtggcagc aatatttata aagtgacggc tcgcgcctat gaccgtaatg
     2521 gcaatagctc taacaatgta cagcttacta ttaccgttct gtcgaatggt caagttgtcg
     2581 accaggttgg ggtaacggac tttacggcgg ataagacttc ggctaaagcg gataacgccg
     2641 ataccattac ttataccgcg acggtgaaaa agaatggggt agctcaggct aatgtccctg
     2701 tttcatttaa tattgtttca ggaactgcaa ctcttggggc aaatagtgcc aaaacggatg
     2761 ctaacggtaa ggcaaccgta acgttgaagt cgagtacgcc aggacaggtc gtcgtgtctg
     2821 ctaaaaccgc ggagatgact tcagcactta atgccagtgc ggttatattt tttgatggtg
     2881 cgactagaca ggtgcagcta caggagtctg ggggaggctc ggtgcggcct ggggagtctc
     2941 ttcgactctc ctgtatagcc gctggaacct tgtttagtgg caaagccgtg ggctggtacc
     3001 gccaggctcc agggaaggac cgcgagctgg tcgcagctct tagtagtggt cgtgaaacat
     3061 actatatagg gcctgtgaag ggccgattca ttatctccag agacgataat aaagacacac
     3121 tgtatctgca aatgaacaac ctaaaacttg aggacacggc catctatacc tgtaggttag
     3181 gcctacattg gggccagggg acccaggtca ccgtctcctc ataataatac tagagccagg
     3241 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     3301 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
     3361 tatatactag tagcggccgc tgcagtccgg caaaaaaggg caaggtgtca ccaccctgcc
     3421 ctttttcttt aaaaccgaaa agattacttc gcgttatgca ggcttcctcg ctcactgact
     3481 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatct
     3541 cgaggttaca ttgtcgatct gttcatggtg aacagcttta aatgcaccaa aaactcgtaa
     3601 aagctctgat gtatctatct tttttacacc gttttcatct gtgcatatgg acagttttcc
     3661 ctttgatatc taacggtgaa cagttgttct acttttgttt gttagtcttg atgcttcact
     3721 gatagataca agagccataa gaacctcaga tccttccgta tttagccagt atgttctcta
     3781 gtgtggttcg ttgtttttgc gtgagccatg agaacgaacc attgagatca tgcttacttt
     3841 gcatgtcact caaaaatttt gcctcaaaac tggtgagctg aatttttgca gttaaagcat
     3901 cgtgtagtgt ttttcttagt ccgttacgta ggtaggaatc tgatgtaatg gttgttggta
     3961 ttttgtcacc attcattttt atctggttgt tctcaagttc ggttacgaga tccatttgtc
     4021 tatctagttc aacttggaaa atcaacgtat cagtcgggcg gcctcgctta tcaaccacca
     4081 atttcatatt gctgtaagtg tttaaatctt tacttattgg tttcaaaacc cattggttaa
     4141 gccttttaaa ctcatggtag ttattttcaa gcattaacat gaacttaaat tcatcaaggc
     4201 taatctctat atttgccttg tgagttttct tttgtgttag ttcttttaat aaccactcat
     4261 aaatcctcat agagtatttg ttttcaaaag acttaacatg ttccagatta tattttatga
     4321 atttttttaa ctggaaaaga taaggcaata tctcttcact aaaaactaat tctaattttt
     4381 cgcttgagaa cttggcatag tttgtccact ggaaaatctc aaagccttta accaaaggat
     4441 tcctgatttc cacagttctc gtcatcagct ctctggttgc tttagctaat acaccataag
     4501 cattttccct actgatgttc atcatctgag cgtattggtt ataagtgaac gataccgtcc
     4561 gttctttcct tgtagggttt tcaatcgtgg ggttgagtag tgccacacag cataaaatta
     4621 gcttggtttc atgctccgtt aagtcatagc gactaatcgc tagttcattt gctttgaaaa
     4681 caactaattc agacatacat ctcaattggt ctaggtgatt ttaatcacta taccaattga
     4741 gatgggctag tcaatgataa ttacatgtcc ttttcctttg agttgtgggt atctgtaaat
     4801 tctgctagac ctttgctgga aaacttgtaa attctgctag accctctgta aattccgcta
     4861 gacctttgtg tgtttttttt gtttatattc aagtggttat aatttataga ataaagaaag
     4921 aataaaaaaa gataaaaaga atagatccca gccctgtgta taactcacta ctttagtcag
     4981 ttccgcagta ttacaaaagg atgtcgcaaa cgctgtttgc tcctctacaa aacagacctt
     5041 aaaaccctaa aggcttaagt agcaccctcg caagctcggg caaatcgctg aatattcctt
     5101 ttgtctccga ccatcaggca cctgagtcgc tgtctttttc gtgacattca gttcgctgcg
     5161 ctcacggctc tggcagtgaa tgggggtaaa tggcactaca ggcgcctttt atggattcat
     5221 gcaaggaaac tacccataat acaagaaaag cccgtcacgg gcttctcagg gcgttttatg
     5281 gcgggtctgc tatgtggtgc tatctgactt tttgctgttc agcagttcct gccctctgat
     5341 tttccagtct gaccacttcg gattatcccg tgacaggtca ttcagactgg ctaatgcacc
     5401 cagtaaggca gcggtatcat caacaggctt acccgtctta ctgtccctag tgcttggatt
     5461 ctcaccaata aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca gatggagttc
     5521 tgaggtcatt actggatcta tcaacaggag tccaagcgag ctcgtaaact tggtctgaca
     5581 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     5641 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     5701 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     5761 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     5821 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     5881 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     5941 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     6001 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     6061 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     6121 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     6181 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     6241 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     6301 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     6361 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     6421 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     6481 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     6541 ttccgcgcac atttcccatg gtgccacctg acgtctaaga aaccattatt atcatgacat
     6601 taacctataa aaataggcgt atcacgaggc agaatttcag ataaaaaaaa tccttagctt
     6661 tcgctaagga tgatttctgg aattcgcggc cgcttctaga g
//
LOCUS       pDSG291                 6224 bp    DNA     circular SYN 14-JUN-2018
DEFINITION  Synthetic plasmid.
ACCESSION   pDSG291
VERSION
KEYWORDS    .
SOURCE      Synthetic plasmid
  ORGANISM  Synthetic plasmid
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 6224)
  AUTHORS   Glass,D.S. and Riedel-Kruse,I.H.
  TITLE     A synthetic bacterial cell-cell adhesion toolbox for programming
            multicellular morphologies and patterns
  JOURNAL   Cell (2018) In press
REFERENCE   2  (bases 1 to 6224)
  AUTHORS   Glass,D.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2018) Bioengineering, Stanford University, 318
            Campus Drive, Clark Center E350, Stanford, CA 94305, USA
COMMENT     Bankit Comment: ALT EMAIL:dsglass@gmail.com
            Bankit Comment: Vecscreen Comment:Submitter says that this sequence
            represents a Cloning Vector
            Bankit Comment: TOTAL # OF SEQS:83.
FEATURES             Location/Qualifiers
     source          1..6224
                     /organism="Synthetic plasmid"
                     /mol_type="other DNA"
                     /db_xref="taxon:1440148"
     misc_feature    1..689
                     /label=BBa_I732084
     misc_feature    1..36
                     /label=pLacIQ (BBa_I4032, 1nt deletion)
     misc_feature    45..56
                     /label=BBa_J04500(1)
     misc_feature    45..56
                     /label=RBS (BBa_B0034)(1)
     CDS             63..686
                     /codon_start=1
                     /transl_table=11
                     /product="tetR ORF (BBa_I732080)"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     misc_feature    698..826
                     /label=Double terminator (BBa_B0015)
     misc_feature    835..896
                     /label=BBa_J13002
     misc_feature    835..888
                     /label=pTet (BBa_R0040)
     misc_feature    897..908
                     /label=BBa_J04500
     misc_feature    897..908
                     /label=RBS (BBa_B0034)
     CDS             915..2882
                     /codon_start=1
                     /transl_table=11
                     /product="Neae_removedRestrictionsites"
                     /translation="MITHGCYTRTRHKHKLKKTLIMLSAGLGLFFYVNQNSFANGENY
                     FKLGSDSKLLTHDSYQNRLFYTLKTGETVADLSKSQDINLSTIWSLNKHLYSSESEMM
                     KAAPGQQIILPLKKLPFEYSALPLLGSAPLVAAGGVAGHTNKLTKMSPDVTKSNMTDD
                     KALNYAAQQAASLGSQLQSRSLNGDYAKDTALGIAGNQASSQLQAWLQHYGTAEVNLQ
                     SGNNFDGSSLDFLLPFYDSEKMLAFGQVGARYIDSRFTANLGAGQRFFLPANMLGYNV
                     FIDQDFSGDNTRLGIGGEYWRDYFKSSVNGYFRMSGWHESYNKKDYDERPANGFDIRF
                     NGYLPSYPALGAKLIYEQYYGDNVALFNSDKLQSNPGAATVGVNYTPIPLVTMGIDYR
                     HGTGNENDLLYSMQFRYQFDKSWSQQIEPQYVNELRTLSGSRYDLVQRNNNIILEYKK
                     QDILSLNIPHDINGTEHSTQKIQLIVKSKYGLDRIVWDDSALRSQGGQIQHSGSQSAQ
                     DYQAILPAYVQGGSNIYKVTARAYDRNGNSSNNVQLTITVLSNGQVVDQVGVTDFTAD
                     KTSAKADNADTITYTATVKKNGVAQANVPVSFNIVSGTATLGANSAKTDANGKATVTL
                     KSSTPGQVVVSAKTAEMTSALNASAVIFFDGA"
     misc_feature    2883..2908
                     /label=RFC[23] biobrick suffix (partial)
     misc_feature    2909..2980
                     /label=his operon terminator
     misc_feature    complement(3044..3061)
                     /label=VR primer site
     CDS             complement(3269..4219)
                     /codon_start=1
                     /transl_table=11
                     /product="repA"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRK
                     ERTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAK
                     FSSEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHK
                     ANIEISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTL
                     IFQVELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTS
                     FEAKFLSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     misc_feature    complement(4223..4235)
                     /label=RBS for repA
     regulatory      complement(4240..4259)
                     /regulatory_class="promoter"
                     /label=repA promoter
     CDS             complement(5105..5965)
                     /codon_start=1
                     /transl_table=11
                     /product="AmpR"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     misc_feature    complement(5966..6044)
                     /label=Promoter/RBS for AmpR
     misc_feature    6085..6105
                     /label=VF2 primer site
     misc_feature    6203..6224
                     /label=BioBricks prefix
BASE COUNT     1705 a   1315 c   1362 g   1842 t
ORIGIN      
        1 tggtgcaaaa cctttgcggt atggcatgat agcgcctact agagaaagag gagaaatact
       61 agatgtccag attagataaa agtaaagtga ttaacagcgc attagagctg cttaatgagg
      121 tcggaatcga aggtttaaca acccgtaaac tcgcccagaa gctaggtgta gagcagccta
      181 cattgtattg gcatgtaaaa aataagcggg ctttgctcga cgccttagcc attgagatgt
      241 tagataggca ccatactcac ttttgccctt tagaagggga aagctggcaa gattttttac
      301 gtaataacgc taaaagtttt agatgtgctt tactaagtca tcgcgatgga gcaaaagtac
      361 atttaggtac acggcctaca gaaaaacagt atgaaactct cgaaaatcaa ttagcctttt
      421 tatgccaaca aggtttttca ctagagaatg cattatatgc actcagcgct gtggggcatt
      481 ttactttagg ttgcgtattg gaagatcaag agcatcaagt cgctaaagaa gaaagggaaa
      541 cacctactac tgatagtatg ccgccattat tacgacaagc tatcgaatta tttgatcacc
      601 aaggtgcaga gccagccttc ttattcggcc ttgaattgat catatgcgga ttagaaaaac
      661 aacttaaatg tgaaagtggg tcctaataat actagagcca ggcatcaaat aaaacgaaag
      721 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
      781 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agagtcccta
      841 tcagtgatag agattgacat ccctatcagt gatagagata ctgagcacta ctagagaaag
      901 aggagaaata ctagatgatt actcatggtt gttatacccg gacccggcac aagcataagc
      961 taaaaaaaac attgattatg cttagtgctg gtttaggatt gtttttttat gttaatcaga
     1021 actcatttgc aaatggtgaa aattatttta aattgggttc ggattcaaaa ctgttaactc
     1081 atgatagcta tcagaatcgc cttttttata cgttgaaaac tggtgaaact gttgccgatc
     1141 tttctaaatc gcaagatatt aatttatcga cgatttggtc gttgaataag catttataca
     1201 gttctgaaag cgaaatgatg aaggccgcgc ctggtcagca gatcattttg ccactcaaaa
     1261 aacttccctt tgaatacagt gcactaccac ttttaggttc ggcacctctt gttgctgcgg
     1321 gtggtgttgc tggtcacacg aataaactga ctaaaatgtc cccggacgtg accaaaagca
     1381 acatgaccga tgacaaggca ttaaattatg cggcacaaca ggcggcgagt ctcggtagcc
     1441 agcttcagtc gcgatctctg aacggcgatt acgcgaaaga taccgctctt ggtatcgctg
     1501 gtaaccaggc ttcgtcacag ttgcaggcct ggttacaaca ttatggaacg gcagaggtta
     1561 atctgcaaag tggtaataac tttgacggta gttcactgga cttcttatta ccgttctatg
     1621 attccgaaaa aatgctggca tttggtcagg tcggagcgcg ttacattgac tcccgcttta
     1681 cggcaaattt aggtgcgggt cagcgttttt tccttcctgc aaacatgttg ggctataacg
     1741 tcttcattga tcaggatttt tctggtgata atacccgttt aggtattggt ggcgaatact
     1801 ggcgagacta tttcaaaagt agcgttaacg gctatttccg catgagcggc tggcatgagt
     1861 catacaataa gaaagactat gatgagcgcc cagcaaatgg cttcgatatc cgttttaatg
     1921 gctatctacc gtcatatccg gcattaggcg ccaagctgat atatgagcag tattatggtg
     1981 ataatgttgc tttgtttaat tctgataagc tgcaatcgaa tcctggtgcg gcgaccgttg
     2041 gtgtaaacta tactccgatt cctctggtga cgatggggat cgattaccgt catggtacgg
     2101 gtaatgaaaa tgatctcctt tactcaatgc agttccgtta tcagtttgat aaatcgtggt
     2161 ctcagcaaat tgaaccacag tatgttaacg agttaagaac attatcaggc agccgttacg
     2221 atctggttca gcgtaataac aatattattc tggagtacaa gaagcaggat attctttctc
     2281 tgaatattcc gcatgatatt aatggtactg aacacagtac gcagaagatt cagttgatcg
     2341 ttaagagcaa atacggtctg gatcgtatcg tctgggatga tagtgcatta cgcagtcagg
     2401 gcggtcagat tcagcatagc ggaagccaaa gcgcacaaga ctaccaggct attttgcctg
     2461 cttatgtgca aggtggcagc aatatttata aagtgacggc tcgcgcctat gaccgtaatg
     2521 gcaatagctc taacaatgta cagcttacta ttaccgttct gtcgaatggt caagttgtcg
     2581 accaggttgg ggtaacggac tttacggcgg ataagacttc ggctaaagcg gataacgccg
     2641 ataccattac ttataccgcg acggtgaaaa agaatggggt agctcaggct aatgtccctg
     2701 tttcatttaa tattgtttca ggaactgcaa ctcttggggc aaatagtgcc aaaacggatg
     2761 ctaacggtaa ggcaaccgta acgttgaagt cgagtacgcc aggacaggtc gtcgtgtctg
     2821 ctaaaaccgc ggagatgact tcagcactta atgccagtgc ggttatattt tttgatggtg
     2881 cgactagtta ataaagcggc cgctgcagtc cggcaaaaaa gggcaaggtg tcaccaccct
     2941 gccctttttc tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg
     3001 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     3061 tctcgaggtt acattgtcga tctgttcatg gtgaacagct ttaaatgcac caaaaactcg
     3121 taaaagctct gatgtatcta tcttttttac accgttttca tctgtgcata tggacagttt
     3181 tccctttgat atctaacggt gaacagttgt tctacttttg tttgttagtc ttgatgcttc
     3241 actgatagat acaagagcca taagaacctc agatccttcc gtatttagcc agtatgttct
     3301 ctagtgtggt tcgttgtttt tgcgtgagcc atgagaacga accattgaga tcatgcttac
     3361 tttgcatgtc actcaaaaat tttgcctcaa aactggtgag ctgaattttt gcagttaaag
     3421 catcgtgtag tgtttttctt agtccgttac gtaggtagga atctgatgta atggttgttg
     3481 gtattttgtc accattcatt tttatctggt tgttctcaag ttcggttacg agatccattt
     3541 gtctatctag ttcaacttgg aaaatcaacg tatcagtcgg gcggcctcgc ttatcaacca
     3601 ccaatttcat attgctgtaa gtgtttaaat ctttacttat tggtttcaaa acccattggt
     3661 taagcctttt aaactcatgg tagttatttt caagcattaa catgaactta aattcatcaa
     3721 ggctaatctc tatatttgcc ttgtgagttt tcttttgtgt tagttctttt aataaccact
     3781 cataaatcct catagagtat ttgttttcaa aagacttaac atgttccaga ttatatttta
     3841 tgaatttttt taactggaaa agataaggca atatctcttc actaaaaact aattctaatt
     3901 tttcgcttga gaacttggca tagtttgtcc actggaaaat ctcaaagcct ttaaccaaag
     3961 gattcctgat ttccacagtt ctcgtcatca gctctctggt tgctttagct aatacaccat
     4021 aagcattttc cctactgatg ttcatcatct gagcgtattg gttataagtg aacgataccg
     4081 tccgttcttt ccttgtaggg ttttcaatcg tggggttgag tagtgccaca cagcataaaa
     4141 ttagcttggt ttcatgctcc gttaagtcat agcgactaat cgctagttca tttgctttga
     4201 aaacaactaa ttcagacata catctcaatt ggtctaggtg attttaatca ctataccaat
     4261 tgagatgggc tagtcaatga taattacatg tccttttcct ttgagttgtg ggtatctgta
     4321 aattctgcta gacctttgct ggaaaacttg taaattctgc tagaccctct gtaaattccg
     4381 ctagaccttt gtgtgttttt tttgtttata ttcaagtggt tataatttat agaataaaga
     4441 aagaataaaa aaagataaaa agaatagatc ccagccctgt gtataactca ctactttagt
     4501 cagttccgca gtattacaaa aggatgtcgc aaacgctgtt tgctcctcta caaaacagac
     4561 cttaaaaccc taaaggctta agtagcaccc tcgcaagctc gggcaaatcg ctgaatattc
     4621 cttttgtctc cgaccatcag gcacctgagt cgctgtcttt ttcgtgacat tcagttcgct
     4681 gcgctcacgg ctctggcagt gaatgggggt aaatggcact acaggcgcct tttatggatt
     4741 catgcaagga aactacccat aatacaagaa aagcccgtca cgggcttctc agggcgtttt
     4801 atggcgggtc tgctatgtgg tgctatctga ctttttgctg ttcagcagtt cctgccctct
     4861 gattttccag tctgaccact tcggattatc ccgtgacagg tcattcagac tggctaatgc
     4921 acccagtaag gcagcggtat catcaacagg cttacccgtc ttactgtccc tagtgcttgg
     4981 attctcacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag
     5041 ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgtaa acttggtctg
     5101 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     5161 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     5221 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     5281 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     5341 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     5401 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     5461 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     5521 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     5581 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     5641 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     5701 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     5761 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     5821 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     5881 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     5941 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     6001 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     6061 gggttccgcg cacatttccc atggtgccac ctgacgtcta agaaaccatt attatcatga
     6121 cattaaccta taaaaatagg cgtatcacga ggcagaattt cagataaaaa aaatccttag
     6181 ctttcgctaa ggatgatttc tggaattcgc ggccgcttct agag
//
LOCUS       pDSG289                 6748 bp    DNA     circular SYN 14-JUN-2018
DEFINITION  Synthetic plasmid.
ACCESSION   pDSG289
VERSION
KEYWORDS    .
SOURCE      Synthetic plasmid
  ORGANISM  Synthetic plasmid
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 6748)
  AUTHORS   Glass,D.S. and Riedel-Kruse,I.H.
  TITLE     A synthetic bacterial cell-cell adhesion toolbox for programming
            multicellular morphologies and patterns
  JOURNAL   Cell (2018) In press
REFERENCE   2  (bases 1 to 6748)
  AUTHORS   Glass,D.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2018) Bioengineering, Stanford University, 318
            Campus Drive, Clark Center E350, Stanford, CA 94305, USA
COMMENT     Bankit Comment: ALT EMAIL:dsglass@gmail.com
            Bankit Comment: Vecscreen Comment:Submitter says that this sequence
            represents a Cloning Vector
            Bankit Comment: TOTAL # OF SEQS:83.
FEATURES             Location/Qualifiers
     source          1..6748
                     /organism="Synthetic plasmid"
                     /mol_type="other DNA"
                     /db_xref="taxon:1440148"
     misc_feature    1..689
                     /label=BBa_I732084
     misc_feature    1..36
                     /label=pLacIQ (BBa_I4032, 1nt deletion)
     misc_feature    45..56
                     /label=BBa_J04500(1)
     misc_feature    45..56
                     /label=RBS (BBa_B0034)(1)
     CDS             63..686
                     /codon_start=1
                     /transl_table=11
                     /product="tetR ORF (BBa_I732080)"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     misc_feature    698..826
                     /label=Double terminator (BBa_B0015)
     misc_feature    835..896
                     /label=BBa_J13002
     misc_feature    835..888
                     /label=pTet (BBa_R0040)
     misc_feature    897..908
                     /label=BBa_J04500
     misc_feature    897..908
                     /label=RBS (BBa_B0034)
     CDS             915..2882
                     /codon_start=1
                     /transl_table=11
                     /product="Neae_removedRestrictionsites"
                     /translation="MITHGCYTRTRHKHKLKKTLIMLSAGLGLFFYVNQNSFANGENY
                     FKLGSDSKLLTHDSYQNRLFYTLKTGETVADLSKSQDINLSTIWSLNKHLYSSESEMM
                     KAAPGQQIILPLKKLPFEYSALPLLGSAPLVAAGGVAGHTNKLTKMSPDVTKSNMTDD
                     KALNYAAQQAASLGSQLQSRSLNGDYAKDTALGIAGNQASSQLQAWLQHYGTAEVNLQ
                     SGNNFDGSSLDFLLPFYDSEKMLAFGQVGARYIDSRFTANLGAGQRFFLPANMLGYNV
                     FIDQDFSGDNTRLGIGGEYWRDYFKSSVNGYFRMSGWHESYNKKDYDERPANGFDIRF
                     NGYLPSYPALGAKLIYEQYYGDNVALFNSDKLQSNPGAATVGVNYTPIPLVTMGIDYR
                     HGTGNENDLLYSMQFRYQFDKSWSQQIEPQYVNELRTLSGSRYDLVQRNNNIILEYKK
                     QDILSLNIPHDINGTEHSTQKIQLIVKSKYGLDRIVWDDSALRSQGGQIQHSGSQSAQ
                     DYQAILPAYVQGGSNIYKVTARAYDRNGNSSNNVQLTITVLSNGQVVDQVGVTDFTAD
                     KTSAKADNADTITYTATVKKNGVAQANVPVSFNIVSGTATLGANSAKTDANGKATVTL
                     KSSTPGQVVVSAKTAEMTSALNASAVIFFDGA"
     CDS             2889..3266
                     /codon_start=1
                     /transl_table=11
                     /product="Nanobody against EPEA tag"
                     /translation="-
                     GQLVESGGGSVQAGGSLRLSCAASGIDSSSYCMGWFRQRPGKEREGVARINGLGGVKT
                     AYADSVKDRFTISRDNAENTVYLQMNSLKPEDTAIYYCAAKFSPGYCGGSWSNFGYWG
                     QGTQVTVSS"
     misc_feature    3267..3272
                     /label=Stop codons
     regulatory      3281..3409
                     /regulatory_class="terminator"
                     /label=terminator B0015
     misc_feature    3410..3430
                     /label=biobrick suffix
     misc_feature    3431..3504
                     /label=his operon terminator
     misc_feature    complement(3568..3585)
                     /label=VR primer site
     CDS             complement(3793..4743)
                     /codon_start=1
                     /transl_table=11
                     /product="repA"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRK
                     ERTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAK
                     FSSEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHK
                     ANIEISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTL
                     IFQVELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTS
                     FEAKFLSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     misc_feature    complement(4747..4759)
                     /label=RBS for repA
     regulatory      complement(4764..4783)
                     /regulatory_class="promoter"
                     /label=repA promoter
     CDS             complement(5629..6489)
                     /codon_start=1
                     /transl_table=11
                     /product="AmpR"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     misc_feature    complement(6490..6568)
                     /label=Promoter/RBS for AmpR
     misc_feature    6609..6629
                     /label=VF2 primer site
     misc_feature    6727..6748
                     /label=BioBricks prefix
BASE COUNT     1828 a   1424 c   1511 g   1985 t
ORIGIN      
        1 tggtgcaaaa cctttgcggt atggcatgat agcgcctact agagaaagag gagaaatact
       61 agatgtccag attagataaa agtaaagtga ttaacagcgc attagagctg cttaatgagg
      121 tcggaatcga aggtttaaca acccgtaaac tcgcccagaa gctaggtgta gagcagccta
      181 cattgtattg gcatgtaaaa aataagcggg ctttgctcga cgccttagcc attgagatgt
      241 tagataggca ccatactcac ttttgccctt tagaagggga aagctggcaa gattttttac
      301 gtaataacgc taaaagtttt agatgtgctt tactaagtca tcgcgatgga gcaaaagtac
      361 atttaggtac acggcctaca gaaaaacagt atgaaactct cgaaaatcaa ttagcctttt
      421 tatgccaaca aggtttttca ctagagaatg cattatatgc actcagcgct gtggggcatt
      481 ttactttagg ttgcgtattg gaagatcaag agcatcaagt cgctaaagaa gaaagggaaa
      541 cacctactac tgatagtatg ccgccattat tacgacaagc tatcgaatta tttgatcacc
      601 aaggtgcaga gccagccttc ttattcggcc ttgaattgat catatgcgga ttagaaaaac
      661 aacttaaatg tgaaagtggg tcctaataat actagagcca ggcatcaaat aaaacgaaag
      721 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
      781 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agagtcccta
      841 tcagtgatag agattgacat ccctatcagt gatagagata ctgagcacta ctagagaaag
      901 aggagaaata ctagatgatt actcatggtt gttatacccg gacccggcac aagcataagc
      961 taaaaaaaac attgattatg cttagtgctg gtttaggatt gtttttttat gttaatcaga
     1021 actcatttgc aaatggtgaa aattatttta aattgggttc ggattcaaaa ctgttaactc
     1081 atgatagcta tcagaatcgc cttttttata cgttgaaaac tggtgaaact gttgccgatc
     1141 tttctaaatc gcaagatatt aatttatcga cgatttggtc gttgaataag catttataca
     1201 gttctgaaag cgaaatgatg aaggccgcgc ctggtcagca gatcattttg ccactcaaaa
     1261 aacttccctt tgaatacagt gcactaccac ttttaggttc ggcacctctt gttgctgcgg
     1321 gtggtgttgc tggtcacacg aataaactga ctaaaatgtc cccggacgtg accaaaagca
     1381 acatgaccga tgacaaggca ttaaattatg cggcacaaca ggcggcgagt ctcggtagcc
     1441 agcttcagtc gcgatctctg aacggcgatt acgcgaaaga taccgctctt ggtatcgctg
     1501 gtaaccaggc ttcgtcacag ttgcaggcct ggttacaaca ttatggaacg gcagaggtta
     1561 atctgcaaag tggtaataac tttgacggta gttcactgga cttcttatta ccgttctatg
     1621 attccgaaaa aatgctggca tttggtcagg tcggagcgcg ttacattgac tcccgcttta
     1681 cggcaaattt aggtgcgggt cagcgttttt tccttcctgc aaacatgttg ggctataacg
     1741 tcttcattga tcaggatttt tctggtgata atacccgttt aggtattggt ggcgaatact
     1801 ggcgagacta tttcaaaagt agcgttaacg gctatttccg catgagcggc tggcatgagt
     1861 catacaataa gaaagactat gatgagcgcc cagcaaatgg cttcgatatc cgttttaatg
     1921 gctatctacc gtcatatccg gcattaggcg ccaagctgat atatgagcag tattatggtg
     1981 ataatgttgc tttgtttaat tctgataagc tgcaatcgaa tcctggtgcg gcgaccgttg
     2041 gtgtaaacta tactccgatt cctctggtga cgatggggat cgattaccgt catggtacgg
     2101 gtaatgaaaa tgatctcctt tactcaatgc agttccgtta tcagtttgat aaatcgtggt
     2161 ctcagcaaat tgaaccacag tatgttaacg agttaagaac attatcaggc agccgttacg
     2221 atctggttca gcgtaataac aatattattc tggagtacaa gaagcaggat attctttctc
     2281 tgaatattcc gcatgatatt aatggtactg aacacagtac gcagaagatt cagttgatcg
     2341 ttaagagcaa atacggtctg gatcgtatcg tctgggatga tagtgcatta cgcagtcagg
     2401 gcggtcagat tcagcatagc ggaagccaaa gcgcacaaga ctaccaggct attttgcctg
     2461 cttatgtgca aggtggcagc aatatttata aagtgacggc tcgcgcctat gaccgtaatg
     2521 gcaatagctc taacaatgta cagcttacta ttaccgttct gtcgaatggt caagttgtcg
     2581 accaggttgg ggtaacggac tttacggcgg ataagacttc ggctaaagcg gataacgccg
     2641 ataccattac ttataccgcg acggtgaaaa agaatggggt agctcaggct aatgtccctg
     2701 tttcatttaa tattgtttca ggaactgcaa ctcttggggc aaatagtgcc aaaacggatg
     2761 ctaacggtaa ggcaaccgta acgttgaagt cgagtacgcc aggacaggtc gtcgtgtctg
     2821 ctaaaaccgc ggagatgact tcagcactta atgccagtgc ggttatattt tttgatggtg
     2881 cgactagaca agggcagctt gttgagagcg gggggggaag cgtacaagcc gggggatcgc
     2941 tgcgcttgtc gtgtgcagct tccggtattg attccagctc atactgtatg ggttggtttc
     3001 gtcagcgtcc gggaaaggag cgcgagggtg tcgcacgtat caacgggttg ggcggcgtta
     3061 aaacagctta cgctgactct gttaaagatc gtttcaccat tagtcgtgat aatgcagaaa
     3121 atactgttta tttgcaaatg aactccttga aacccgaaga tacagctatt tattattgtg
     3181 ctgccaagtt tagtccaggt tattgcggag gatcgtggag caatttcgga tactggggtc
     3241 aaggaacgca agtgaccgta tcttcgtaat aatactagag ccaggcatca aataaaacga
     3301 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     3361 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat actagtagcg
     3421 gccgctgcag tccggcaaaa aaacgggcaa ggtgtcacca ccctgccctt tttctttaaa
     3481 accgaaaaga ttacttcgcg ttatgcaggc ttcctcgctc actgactcgc tgcgctcggt
     3541 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatctcga ggttacattg
     3601 tcgatctgtt catggtgaac agctttaaat gcaccaaaaa ctcgtaaaag ctctgatgta
     3661 tctatctttt ttacaccgtt ttcatctgtg catatggaca gttttccctt tgatatctaa
     3721 cggtgaacag ttgttctact tttgtttgtt agtcttgatg cttcactgat agatacaaga
     3781 gccataagaa cctcagatcc ttccgtattt agccagtatg ttctctagtg tggttcgttg
     3841 tttttgcgtg agccatgaga acgaaccatt gagatcatgc ttactttgca tgtcactcaa
     3901 aaattttgcc tcaaaactgg tgagctgaat ttttgcagtt aaagcatcgt gtagtgtttt
     3961 tcttagtccg ttacgtaggt aggaatctga tgtaatggtt gttggtattt tgtcaccatt
     4021 catttttatc tggttgttct caagttcggt tacgagatcc atttgtctat ctagttcaac
     4081 ttggaaaatc aacgtatcag tcgggcggcc tcgcttatca accaccaatt tcatattgct
     4141 gtaagtgttt aaatctttac ttattggttt caaaacccat tggttaagcc ttttaaactc
     4201 atggtagtta ttttcaagca ttaacatgaa cttaaattca tcaaggctaa tctctatatt
     4261 tgccttgtga gttttctttt gtgttagttc ttttaataac cactcataaa tcctcataga
     4321 gtatttgttt tcaaaagact taacatgttc cagattatat tttatgaatt tttttaactg
     4381 gaaaagataa ggcaatatct cttcactaaa aactaattct aatttttcgc ttgagaactt
     4441 ggcatagttt gtccactgga aaatctcaaa gcctttaacc aaaggattcc tgatttccac
     4501 agttctcgtc atcagctctc tggttgcttt agctaataca ccataagcat tttccctact
     4561 gatgttcatc atctgagcgt attggttata agtgaacgat accgtccgtt ctttccttgt
     4621 agggttttca atcgtggggt tgagtagtgc cacacagcat aaaattagct tggtttcatg
     4681 ctccgttaag tcatagcgac taatcgctag ttcatttgct ttgaaaacaa ctaattcaga
     4741 catacatctc aattggtcta ggtgatttta atcactatac caattgagat gggctagtca
     4801 atgataatta catgtccttt tcctttgagt tgtgggtatc tgtaaattct gctagacctt
     4861 tgctggaaaa cttgtaaatt ctgctagacc ctctgtaaat tccgctagac ctttgtgtgt
     4921 tttttttgtt tatattcaag tggttataat ttatagaata aagaaagaat aaaaaaagat
     4981 aaaaagaata gatcccagcc ctgtgtataa ctcactactt tagtcagttc cgcagtatta
     5041 caaaaggatg tcgcaaacgc tgtttgctcc tctacaaaac agaccttaaa accctaaagg
     5101 cttaagtagc accctcgcaa gctcgggcaa atcgctgaat attccttttg tctccgacca
     5161 tcaggcacct gagtcgctgt ctttttcgtg acattcagtt cgctgcgctc acggctctgg
     5221 cagtgaatgg gggtaaatgg cactacaggc gccttttatg gattcatgca aggaaactac
     5281 ccataataca agaaaagccc gtcacgggct tctcagggcg ttttatggcg ggtctgctat
     5341 gtggtgctat ctgacttttt gctgttcagc agttcctgcc ctctgatttt ccagtctgac
     5401 cacttcggat tatcccgtga caggtcattc agactggcta atgcacccag taaggcagcg
     5461 gtatcatcaa caggcttacc cgtcttactg tccctagtgc ttggattctc accaataaaa
     5521 aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact
     5581 ggatctatca acaggagtcc aagcgagctc gtaaacttgg tctgacagtt accaatgctt
     5641 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     5701 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     5761 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     5821 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     5881 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     5941 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     6001 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     6061 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     6121 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     6181 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     6241 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     6301 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     6361 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     6421 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     6481 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     6541 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     6601 tcccatggtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     6661 taggcgtatc acgaggcaga atttcagata aaaaaaatcc ttagctttcg ctaaggatga
     6721 tttctggaat tcgcggccgc ttctagag
//
LOCUS       pDSG288                 6676 bp    DNA     circular SYN 14-JUN-2018
DEFINITION  Synthetic plasmid.
ACCESSION   pDSG288
VERSION
KEYWORDS    .
SOURCE      Synthetic plasmid
  ORGANISM  Synthetic plasmid
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 6676)
  AUTHORS   Glass,D.S. and Riedel-Kruse,I.H.
  TITLE     A synthetic bacterial cell-cell adhesion toolbox for programming
            multicellular morphologies and patterns
  JOURNAL   Cell (2018) In press
REFERENCE   2  (bases 1 to 6676)
  AUTHORS   Glass,D.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2018) Bioengineering, Stanford University, 318
            Campus Drive, Clark Center E350, Stanford, CA 94305, USA
COMMENT     Bankit Comment: ALT EMAIL:dsglass@gmail.com
            Bankit Comment: Vecscreen Comment:Submitter says that this sequence
            represents a Cloning Vector
            Bankit Comment: TOTAL # OF SEQS:83.
FEATURES             Location/Qualifiers
     source          1..6676
                     /organism="Synthetic plasmid"
                     /mol_type="other DNA"
                     /db_xref="taxon:1440148"
     misc_feature    1..689
                     /label=BBa_I732084
     misc_feature    1..36
                     /label=pLacIQ (BBa_I4032, 1nt deletion)
     misc_feature    45..56
                     /label=BBa_J04500(1)
     misc_feature    45..56
                     /label=RBS (BBa_B0034)(1)
     CDS             63..686
                     /codon_start=1
                     /transl_table=11
                     /product="tetR ORF (BBa_I732080)"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     misc_feature    698..826
                     /label=Double terminator (BBa_B0015)
     misc_feature    835..896
                     /label=BBa_J13002
     misc_feature    835..888
                     /label=pTet (BBa_R0040)
     misc_feature    897..908
                     /label=BBa_J04500
     misc_feature    897..908
                     /label=RBS (BBa_B0034)
     CDS             915..2882
                     /codon_start=1
                     /transl_table=11
                     /product="Neae_removedRestrictionsites"
                     /translation="MITHGCYTRTRHKHKLKKTLIMLSAGLGLFFYVNQNSFANGENY
                     FKLGSDSKLLTHDSYQNRLFYTLKTGETVADLSKSQDINLSTIWSLNKHLYSSESEMM
                     KAAPGQQIILPLKKLPFEYSALPLLGSAPLVAAGGVAGHTNKLTKMSPDVTKSNMTDD
                     KALNYAAQQAASLGSQLQSRSLNGDYAKDTALGIAGNQASSQLQAWLQHYGTAEVNLQ
                     SGNNFDGSSLDFLLPFYDSEKMLAFGQVGARYIDSRFTANLGAGQRFFLPANMLGYNV
                     FIDQDFSGDNTRLGIGGEYWRDYFKSSVNGYFRMSGWHESYNKKDYDERPANGFDIRF
                     NGYLPSYPALGAKLIYEQYYGDNVALFNSDKLQSNPGAATVGVNYTPIPLVTMGIDYR
                     HGTGNENDLLYSMQFRYQFDKSWSQQIEPQYVNELRTLSGSRYDLVQRNNNIILEYKK
                     QDILSLNIPHDINGTEHSTQKIQLIVKSKYGLDRIVWDDSALRSQGGQIQHSGSQSAQ
                     DYQAILPAYVQGGSNIYKVTARAYDRNGNSSNNVQLTITVLSNGQVVDQVGVTDFTAD
                     KTSAKADNADTITYTATVKKNGVAQANVPVSFNIVSGTATLGANSAKTDANGKATVTL
                     KSSTPGQVVVSAKTAEMTSALNASAVIFFDGA"
     CDS             2889..3194
                     /codon_start=1
                     /transl_table=11
                     /product="human P53 transactivation domain"
                     /translation="MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLM
                     LSPDDIEQWFTEDPGPDEAPRMPEAAPRVAPAPAAPTPAAPAPAPSWPLSSSVPSQKT
                     "
     misc_feature    3195..3200
                     /label=Stop codons
     regulatory      3209..3337
                     /regulatory_class="terminator"
                     /label=terminator B0015
     misc_feature    3338..3358
                     /label=biobrick suffix
     misc_feature    3359..3432
                     /label=his operon terminator
     misc_feature    complement(3496..3513)
                     /label=VR primer site
     CDS             complement(3721..4671)
                     /codon_start=1
                     /transl_table=11
                     /product="repA"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRK
                     ERTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAK
                     FSSEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHK
                     ANIEISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTL
                     IFQVELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTS
                     FEAKFLSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     misc_feature    complement(4675..4687)
                     /label=RBS for repA
     regulatory      complement(4692..4711)
                     /regulatory_class="promoter"
                     /label=repA promoter
     CDS             complement(5557..6417)
                     /codon_start=1
                     /transl_table=11
                     /product="AmpR"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     misc_feature    complement(6418..6496)
                     /label=Promoter/RBS for AmpR
     misc_feature    6537..6557
                     /label=VF2 primer site
     misc_feature    6655..6676
                     /label=BioBricks prefix
BASE COUNT     1811 a   1445 c   1463 g   1957 t
ORIGIN      
        1 tggtgcaaaa cctttgcggt atggcatgat agcgcctact agagaaagag gagaaatact
       61 agatgtccag attagataaa agtaaagtga ttaacagcgc attagagctg cttaatgagg
      121 tcggaatcga aggtttaaca acccgtaaac tcgcccagaa gctaggtgta gagcagccta
      181 cattgtattg gcatgtaaaa aataagcggg ctttgctcga cgccttagcc attgagatgt
      241 tagataggca ccatactcac ttttgccctt tagaagggga aagctggcaa gattttttac
      301 gtaataacgc taaaagtttt agatgtgctt tactaagtca tcgcgatgga gcaaaagtac
      361 atttaggtac acggcctaca gaaaaacagt atgaaactct cgaaaatcaa ttagcctttt
      421 tatgccaaca aggtttttca ctagagaatg cattatatgc actcagcgct gtggggcatt
      481 ttactttagg ttgcgtattg gaagatcaag agcatcaagt cgctaaagaa gaaagggaaa
      541 cacctactac tgatagtatg ccgccattat tacgacaagc tatcgaatta tttgatcacc
      601 aaggtgcaga gccagccttc ttattcggcc ttgaattgat catatgcgga ttagaaaaac
      661 aacttaaatg tgaaagtggg tcctaataat actagagcca ggcatcaaat aaaacgaaag
      721 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
      781 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agagtcccta
      841 tcagtgatag agattgacat ccctatcagt gatagagata ctgagcacta ctagagaaag
      901 aggagaaata ctagatgatt actcatggtt gttatacccg gacccggcac aagcataagc
      961 taaaaaaaac attgattatg cttagtgctg gtttaggatt gtttttttat gttaatcaga
     1021 actcatttgc aaatggtgaa aattatttta aattgggttc ggattcaaaa ctgttaactc
     1081 atgatagcta tcagaatcgc cttttttata cgttgaaaac tggtgaaact gttgccgatc
     1141 tttctaaatc gcaagatatt aatttatcga cgatttggtc gttgaataag catttataca
     1201 gttctgaaag cgaaatgatg aaggccgcgc ctggtcagca gatcattttg ccactcaaaa
     1261 aacttccctt tgaatacagt gcactaccac ttttaggttc ggcacctctt gttgctgcgg
     1321 gtggtgttgc tggtcacacg aataaactga ctaaaatgtc cccggacgtg accaaaagca
     1381 acatgaccga tgacaaggca ttaaattatg cggcacaaca ggcggcgagt ctcggtagcc
     1441 agcttcagtc gcgatctctg aacggcgatt acgcgaaaga taccgctctt ggtatcgctg
     1501 gtaaccaggc ttcgtcacag ttgcaggcct ggttacaaca ttatggaacg gcagaggtta
     1561 atctgcaaag tggtaataac tttgacggta gttcactgga cttcttatta ccgttctatg
     1621 attccgaaaa aatgctggca tttggtcagg tcggagcgcg ttacattgac tcccgcttta
     1681 cggcaaattt aggtgcgggt cagcgttttt tccttcctgc aaacatgttg ggctataacg
     1741 tcttcattga tcaggatttt tctggtgata atacccgttt aggtattggt ggcgaatact
     1801 ggcgagacta tttcaaaagt agcgttaacg gctatttccg catgagcggc tggcatgagt
     1861 catacaataa gaaagactat gatgagcgcc cagcaaatgg cttcgatatc cgttttaatg
     1921 gctatctacc gtcatatccg gcattaggcg ccaagctgat atatgagcag tattatggtg
     1981 ataatgttgc tttgtttaat tctgataagc tgcaatcgaa tcctggtgcg gcgaccgttg
     2041 gtgtaaacta tactccgatt cctctggtga cgatggggat cgattaccgt catggtacgg
     2101 gtaatgaaaa tgatctcctt tactcaatgc agttccgtta tcagtttgat aaatcgtggt
     2161 ctcagcaaat tgaaccacag tatgttaacg agttaagaac attatcaggc agccgttacg
     2221 atctggttca gcgtaataac aatattattc tggagtacaa gaagcaggat attctttctc
     2281 tgaatattcc gcatgatatt aatggtactg aacacagtac gcagaagatt cagttgatcg
     2341 ttaagagcaa atacggtctg gatcgtatcg tctgggatga tagtgcatta cgcagtcagg
     2401 gcggtcagat tcagcatagc ggaagccaaa gcgcacaaga ctaccaggct attttgcctg
     2461 cttatgtgca aggtggcagc aatatttata aagtgacggc tcgcgcctat gaccgtaatg
     2521 gcaatagctc taacaatgta cagcttacta ttaccgttct gtcgaatggt caagttgtcg
     2581 accaggttgg ggtaacggac tttacggcgg ataagacttc ggctaaagcg gataacgccg
     2641 ataccattac ttataccgcg acggtgaaaa agaatggggt agctcaggct aatgtccctg
     2701 tttcatttaa tattgtttca ggaactgcaa ctcttggggc aaatagtgcc aaaacggatg
     2761 ctaacggtaa ggcaaccgta acgttgaagt cgagtacgcc aggacaggtc gtcgtgtctg
     2821 ctaaaaccgc ggagatgact tcagcactta atgccagtgc ggttatattt tttgatggtg
     2881 cgactagaat ggaggaaccc caatccgatc cttccgtaga gccgccttta tcacaggaga
     2941 cgttctcgga cctttggaaa ttgttacccg agaataatgt tctgtcaccg ttgccctcac
     3001 aagctatgga tgacttaatg ttgtctccag atgatattga acaatggttt acagaggacc
     3061 ccggtccaga tgaagctccg cgcatgccag aagctgcacc ccgtgtcgct cccgctccag
     3121 cagcaccaac tccagcagcg ccagcacctg ccccttcttg gccattgtcg tcttccgtac
     3181 ctagtcagaa aacttaataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg
     3241 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
     3301 actggctcac cttcgggtgg gcctttctgc gtttatatac tagtagcggc cgctgcagtc
     3361 cggcaaaaaa acgggcaagg tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt
     3421 acttcgcgtt atgcaggctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     3481 gcgagcggta tcagctcact caaaggcggt aatctcgagg ttacattgtc gatctgttca
     3541 tggtgaacag ctttaaatgc accaaaaact cgtaaaagct ctgatgtatc tatctttttt
     3601 acaccgtttt catctgtgca tatggacagt tttccctttg atatctaacg gtgaacagtt
     3661 gttctacttt tgtttgttag tcttgatgct tcactgatag atacaagagc cataagaacc
     3721 tcagatcctt ccgtatttag ccagtatgtt ctctagtgtg gttcgttgtt tttgcgtgag
     3781 ccatgagaac gaaccattga gatcatgctt actttgcatg tcactcaaaa attttgcctc
     3841 aaaactggtg agctgaattt ttgcagttaa agcatcgtgt agtgtttttc ttagtccgtt
     3901 acgtaggtag gaatctgatg taatggttgt tggtattttg tcaccattca tttttatctg
     3961 gttgttctca agttcggtta cgagatccat ttgtctatct agttcaactt ggaaaatcaa
     4021 cgtatcagtc gggcggcctc gcttatcaac caccaatttc atattgctgt aagtgtttaa
     4081 atctttactt attggtttca aaacccattg gttaagcctt ttaaactcat ggtagttatt
     4141 ttcaagcatt aacatgaact taaattcatc aaggctaatc tctatatttg ccttgtgagt
     4201 tttcttttgt gttagttctt ttaataacca ctcataaatc ctcatagagt atttgttttc
     4261 aaaagactta acatgttcca gattatattt tatgaatttt tttaactgga aaagataagg
     4321 caatatctct tcactaaaaa ctaattctaa tttttcgctt gagaacttgg catagtttgt
     4381 ccactggaaa atctcaaagc ctttaaccaa aggattcctg atttccacag ttctcgtcat
     4441 cagctctctg gttgctttag ctaatacacc ataagcattt tccctactga tgttcatcat
     4501 ctgagcgtat tggttataag tgaacgatac cgtccgttct ttccttgtag ggttttcaat
     4561 cgtggggttg agtagtgcca cacagcataa aattagcttg gtttcatgct ccgttaagtc
     4621 atagcgacta atcgctagtt catttgcttt gaaaacaact aattcagaca tacatctcaa
     4681 ttggtctagg tgattttaat cactatacca attgagatgg gctagtcaat gataattaca
     4741 tgtccttttc ctttgagttg tgggtatctg taaattctgc tagacctttg ctggaaaact
     4801 tgtaaattct gctagaccct ctgtaaattc cgctagacct ttgtgtgttt tttttgttta
     4861 tattcaagtg gttataattt atagaataaa gaaagaataa aaaaagataa aaagaataga
     4921 tcccagccct gtgtataact cactacttta gtcagttccg cagtattaca aaaggatgtc
     4981 gcaaacgctg tttgctcctc tacaaaacag accttaaaac cctaaaggct taagtagcac
     5041 cctcgcaagc tcgggcaaat cgctgaatat tccttttgtc tccgaccatc aggcacctga
     5101 gtcgctgtct ttttcgtgac attcagttcg ctgcgctcac ggctctggca gtgaatgggg
     5161 gtaaatggca ctacaggcgc cttttatgga ttcatgcaag gaaactaccc ataatacaag
     5221 aaaagcccgt cacgggcttc tcagggcgtt ttatggcggg tctgctatgt ggtgctatct
     5281 gactttttgc tgttcagcag ttcctgccct ctgattttcc agtctgacca cttcggatta
     5341 tcccgtgaca ggtcattcag actggctaat gcacccagta aggcagcggt atcatcaaca
     5401 ggcttacccg tcttactgtc cctagtgctt ggattctcac caataaaaaa cgcccggcgg
     5461 caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg atctatcaac
     5521 aggagtccaa gcgagctcgt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     5581 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     5641 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     5701 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     5761 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     5821 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     5881 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     5941 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     6001 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     6061 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     6121 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     6181 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     6241 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     6301 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     6361 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     6421 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     6481 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc ccatggtgcc
     6541 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     6601 gaggcagaat ttcagataaa aaaaatcctt agctttcgct aaggatgatt tctggaattc
     6661 gcggccgctt ctagag
//
LOCUS       pDSG287                 6382 bp    DNA     circular SYN 14-JUN-2018
DEFINITION  Synthetic plasmid.
ACCESSION   pDSG287
VERSION
KEYWORDS    .
SOURCE      Synthetic plasmid
  ORGANISM  Synthetic plasmid
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 6382)
  AUTHORS   Glass,D.S. and Riedel-Kruse,I.H.
  TITLE     A synthetic bacterial cell-cell adhesion toolbox for programming
            multicellular morphologies and patterns
  JOURNAL   Cell (2018) In press
REFERENCE   2  (bases 1 to 6382)
  AUTHORS   Glass,D.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2018) Bioengineering, Stanford University, 318
            Campus Drive, Clark Center E350, Stanford, CA 94305, USA
COMMENT     Bankit Comment: ALT EMAIL:dsglass@gmail.com
            Bankit Comment: Vecscreen Comment:Submitter says that this sequence
            represents a Cloning Vector
            Bankit Comment: TOTAL # OF SEQS:83.
FEATURES             Location/Qualifiers
     source          1..6382
                     /organism="Synthetic plasmid"
                     /mol_type="other DNA"
                     /db_xref="taxon:1440148"
     misc_feature    1..689
                     /label=BBa_I732084
     misc_feature    1..36
                     /label=pLacIQ (BBa_I4032, 1nt deletion)
     misc_feature    45..56
                     /label=BBa_J04500(1)
     misc_feature    45..56
                     /label=RBS (BBa_B0034)(1)
     CDS             63..686
                     /codon_start=1
                     /transl_table=11
                     /product="tetR ORF (BBa_I732080)"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     misc_feature    698..826
                     /label=Double terminator (BBa_B0015)
     misc_feature    835..896
                     /label=BBa_J13002
     misc_feature    835..888
                     /label=pTet (BBa_R0040)
     misc_feature    897..908
                     /label=BBa_J04500
     misc_feature    897..908
                     /label=RBS (BBa_B0034)
     CDS             915..2882
                     /codon_start=1
                     /transl_table=11
                     /product="Neae_removedRestrictionsites"
                     /translation="MITHGCYTRTRHKHKLKKTLIMLSAGLGLFFYVNQNSFANGENY
                     FKLGSDSKLLTHDSYQNRLFYTLKTGETVADLSKSQDINLSTIWSLNKHLYSSESEMM
                     KAAPGQQIILPLKKLPFEYSALPLLGSAPLVAAGGVAGHTNKLTKMSPDVTKSNMTDD
                     KALNYAAQQAASLGSQLQSRSLNGDYAKDTALGIAGNQASSQLQAWLQHYGTAEVNLQ
                     SGNNFDGSSLDFLLPFYDSEKMLAFGQVGARYIDSRFTANLGAGQRFFLPANMLGYNV
                     FIDQDFSGDNTRLGIGGEYWRDYFKSSVNGYFRMSGWHESYNKKDYDERPANGFDIRF
                     NGYLPSYPALGAKLIYEQYYGDNVALFNSDKLQSNPGAATVGVNYTPIPLVTMGIDYR
                     HGTGNENDLLYSMQFRYQFDKSWSQQIEPQYVNELRTLSGSRYDLVQRNNNIILEYKK
                     QDILSLNIPHDINGTEHSTQKIQLIVKSKYGLDRIVWDDSALRSQGGQIQHSGSQSAQ
                     DYQAILPAYVQGGSNIYKVTARAYDRNGNSSNNVQLTITVLSNGQVVDQVGVTDFTAD
                     KTSAKADNADTITYTATVKKNGVAQANVPVSFNIVSGTATLGANSAKTDANGKATVTL
                     KSSTPGQVVVSAKTAEMTSALNASAVIFFDGA"
     CDS             2889..2900
                     /codon_start=1
                     /transl_table=11
                     /product="EPEA peptide (C-termial of alpha synuclein)"
                     /translation="-PEA"
     misc_feature    2901..2906
                     /label=Stop codons
     regulatory      2915..3043
                     /regulatory_class="terminator"
                     /label=terminator B0015
     misc_feature    3044..3064
                     /label=biobrick suffix
     misc_feature    3065..3138
                     /label=his operon terminator
     misc_feature    complement(3202..3219)
                     /label=VR primer site
     CDS             complement(3427..4377)
                     /codon_start=1
                     /transl_table=11
                     /product="repA"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRK
                     ERTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAK
                     FSSEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHK
                     ANIEISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTL
                     IFQVELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTS
                     FEAKFLSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     misc_feature    complement(4381..4393)
                     /label=RBS for repA
     regulatory      complement(4398..4417)
                     /regulatory_class="promoter"
                     /label=repA promoter
     CDS             complement(5263..6123)
                     /codon_start=1
                     /transl_table=11
                     /product="AmpR"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     misc_feature    complement(6124..6202)
                     /label=Promoter/RBS for AmpR
     misc_feature    6243..6263
                     /label=VF2 primer site
     misc_feature    6361..6382
                     /label=BioBricks prefix
BASE COUNT     1744 a   1352 c   1400 g   1886 t
ORIGIN      
        1 tggtgcaaaa cctttgcggt atggcatgat agcgcctact agagaaagag gagaaatact
       61 agatgtccag attagataaa agtaaagtga ttaacagcgc attagagctg cttaatgagg
      121 tcggaatcga aggtttaaca acccgtaaac tcgcccagaa gctaggtgta gagcagccta
      181 cattgtattg gcatgtaaaa aataagcggg ctttgctcga cgccttagcc attgagatgt
      241 tagataggca ccatactcac ttttgccctt tagaagggga aagctggcaa gattttttac
      301 gtaataacgc taaaagtttt agatgtgctt tactaagtca tcgcgatgga gcaaaagtac
      361 atttaggtac acggcctaca gaaaaacagt atgaaactct cgaaaatcaa ttagcctttt
      421 tatgccaaca aggtttttca ctagagaatg cattatatgc actcagcgct gtggggcatt
      481 ttactttagg ttgcgtattg gaagatcaag agcatcaagt cgctaaagaa gaaagggaaa
      541 cacctactac tgatagtatg ccgccattat tacgacaagc tatcgaatta tttgatcacc
      601 aaggtgcaga gccagccttc ttattcggcc ttgaattgat catatgcgga ttagaaaaac
      661 aacttaaatg tgaaagtggg tcctaataat actagagcca ggcatcaaat aaaacgaaag
      721 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
      781 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agagtcccta
      841 tcagtgatag agattgacat ccctatcagt gatagagata ctgagcacta ctagagaaag
      901 aggagaaata ctagatgatt actcatggtt gttatacccg gacccggcac aagcataagc
      961 taaaaaaaac attgattatg cttagtgctg gtttaggatt gtttttttat gttaatcaga
     1021 actcatttgc aaatggtgaa aattatttta aattgggttc ggattcaaaa ctgttaactc
     1081 atgatagcta tcagaatcgc cttttttata cgttgaaaac tggtgaaact gttgccgatc
     1141 tttctaaatc gcaagatatt aatttatcga cgatttggtc gttgaataag catttataca
     1201 gttctgaaag cgaaatgatg aaggccgcgc ctggtcagca gatcattttg ccactcaaaa
     1261 aacttccctt tgaatacagt gcactaccac ttttaggttc ggcacctctt gttgctgcgg
     1321 gtggtgttgc tggtcacacg aataaactga ctaaaatgtc cccggacgtg accaaaagca
     1381 acatgaccga tgacaaggca ttaaattatg cggcacaaca ggcggcgagt ctcggtagcc
     1441 agcttcagtc gcgatctctg aacggcgatt acgcgaaaga taccgctctt ggtatcgctg
     1501 gtaaccaggc ttcgtcacag ttgcaggcct ggttacaaca ttatggaacg gcagaggtta
     1561 atctgcaaag tggtaataac tttgacggta gttcactgga cttcttatta ccgttctatg
     1621 attccgaaaa aatgctggca tttggtcagg tcggagcgcg ttacattgac tcccgcttta
     1681 cggcaaattt aggtgcgggt cagcgttttt tccttcctgc aaacatgttg ggctataacg
     1741 tcttcattga tcaggatttt tctggtgata atacccgttt aggtattggt ggcgaatact
     1801 ggcgagacta tttcaaaagt agcgttaacg gctatttccg catgagcggc tggcatgagt
     1861 catacaataa gaaagactat gatgagcgcc cagcaaatgg cttcgatatc cgttttaatg
     1921 gctatctacc gtcatatccg gcattaggcg ccaagctgat atatgagcag tattatggtg
     1981 ataatgttgc tttgtttaat tctgataagc tgcaatcgaa tcctggtgcg gcgaccgttg
     2041 gtgtaaacta tactccgatt cctctggtga cgatggggat cgattaccgt catggtacgg
     2101 gtaatgaaaa tgatctcctt tactcaatgc agttccgtta tcagtttgat aaatcgtggt
     2161 ctcagcaaat tgaaccacag tatgttaacg agttaagaac attatcaggc agccgttacg
     2221 atctggttca gcgtaataac aatattattc tggagtacaa gaagcaggat attctttctc
     2281 tgaatattcc gcatgatatt aatggtactg aacacagtac gcagaagatt cagttgatcg
     2341 ttaagagcaa atacggtctg gatcgtatcg tctgggatga tagtgcatta cgcagtcagg
     2401 gcggtcagat tcagcatagc ggaagccaaa gcgcacaaga ctaccaggct attttgcctg
     2461 cttatgtgca aggtggcagc aatatttata aagtgacggc tcgcgcctat gaccgtaatg
     2521 gcaatagctc taacaatgta cagcttacta ttaccgttct gtcgaatggt caagttgtcg
     2581 accaggttgg ggtaacggac tttacggcgg ataagacttc ggctaaagcg gataacgccg
     2641 ataccattac ttataccgcg acggtgaaaa agaatggggt agctcaggct aatgtccctg
     2701 tttcatttaa tattgtttca ggaactgcaa ctcttggggc aaatagtgcc aaaacggatg
     2761 ctaacggtaa ggcaaccgta acgttgaagt cgagtacgcc aggacaggtc gtcgtgtctg
     2821 ctaaaaccgc ggagatgact tcagcactta atgccagtgc ggttatattt tttgatggtg
     2881 cgactagaga accagaggct taataatact agagccaggc atcaaataaa acgaaaggct
     2941 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
     3001 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactagt agcggccgct
     3061 gcagtccggc aaaaaaacgg gcaaggtgtc accaccctgc cctttttctt taaaaccgaa
     3121 aagattactt cgcgttatgc aggcttcctc gctcactgac tcgctgcgct cggtcgttcg
     3181 gctgcggcga gcggtatcag ctcactcaaa ggcggtaatc tcgaggttac attgtcgatc
     3241 tgttcatggt gaacagcttt aaatgcacca aaaactcgta aaagctctga tgtatctatc
     3301 ttttttacac cgttttcatc tgtgcatatg gacagttttc cctttgatat ctaacggtga
     3361 acagttgttc tacttttgtt tgttagtctt gatgcttcac tgatagatac aagagccata
     3421 agaacctcag atccttccgt atttagccag tatgttctct agtgtggttc gttgtttttg
     3481 cgtgagccat gagaacgaac cattgagatc atgcttactt tgcatgtcac tcaaaaattt
     3541 tgcctcaaaa ctggtgagct gaatttttgc agttaaagca tcgtgtagtg tttttcttag
     3601 tccgttacgt aggtaggaat ctgatgtaat ggttgttggt attttgtcac cattcatttt
     3661 tatctggttg ttctcaagtt cggttacgag atccatttgt ctatctagtt caacttggaa
     3721 aatcaacgta tcagtcgggc ggcctcgctt atcaaccacc aatttcatat tgctgtaagt
     3781 gtttaaatct ttacttattg gtttcaaaac ccattggtta agccttttaa actcatggta
     3841 gttattttca agcattaaca tgaacttaaa ttcatcaagg ctaatctcta tatttgcctt
     3901 gtgagttttc ttttgtgtta gttcttttaa taaccactca taaatcctca tagagtattt
     3961 gttttcaaaa gacttaacat gttccagatt atattttatg aattttttta actggaaaag
     4021 ataaggcaat atctcttcac taaaaactaa ttctaatttt tcgcttgaga acttggcata
     4081 gtttgtccac tggaaaatct caaagccttt aaccaaagga ttcctgattt ccacagttct
     4141 cgtcatcagc tctctggttg ctttagctaa tacaccataa gcattttccc tactgatgtt
     4201 catcatctga gcgtattggt tataagtgaa cgataccgtc cgttctttcc ttgtagggtt
     4261 ttcaatcgtg gggttgagta gtgccacaca gcataaaatt agcttggttt catgctccgt
     4321 taagtcatag cgactaatcg ctagttcatt tgctttgaaa acaactaatt cagacataca
     4381 tctcaattgg tctaggtgat tttaatcact ataccaattg agatgggcta gtcaatgata
     4441 attacatgtc cttttccttt gagttgtggg tatctgtaaa ttctgctaga cctttgctgg
     4501 aaaacttgta aattctgcta gaccctctgt aaattccgct agacctttgt gtgttttttt
     4561 tgtttatatt caagtggtta taatttatag aataaagaaa gaataaaaaa agataaaaag
     4621 aatagatccc agccctgtgt ataactcact actttagtca gttccgcagt attacaaaag
     4681 gatgtcgcaa acgctgtttg ctcctctaca aaacagacct taaaacccta aaggcttaag
     4741 tagcaccctc gcaagctcgg gcaaatcgct gaatattcct tttgtctccg accatcaggc
     4801 acctgagtcg ctgtcttttt cgtgacattc agttcgctgc gctcacggct ctggcagtga
     4861 atgggggtaa atggcactac aggcgccttt tatggattca tgcaaggaaa ctacccataa
     4921 tacaagaaaa gcccgtcacg ggcttctcag ggcgttttat ggcgggtctg ctatgtggtg
     4981 ctatctgact ttttgctgtt cagcagttcc tgccctctga ttttccagtc tgaccacttc
     5041 ggattatccc gtgacaggtc attcagactg gctaatgcac ccagtaaggc agcggtatca
     5101 tcaacaggct tacccgtctt actgtcccta gtgcttggat tctcaccaat aaaaaacgcc
     5161 cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct
     5221 atcaacagga gtccaagcga gctcgtaaac ttggtctgac agttaccaat gcttaatcag
     5281 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     5341 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     5401 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     5461 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     5521 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     5581 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     5641 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     5701 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     5761 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     5821 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     5881 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     5941 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     6001 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     6061 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     6121 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     6181 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttcccat
     6241 ggtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     6301 tatcacgagg cagaatttca gataaaaaaa atccttagct ttcgctaagg atgatttctg
     6361 gaattcgcgg ccgcttctag ag
//
LOCUS       pDSG312                 6342 bp    DNA     circular SYN 14-JUN-2018
DEFINITION  Synthetic plasmid.
ACCESSION   pDSG312
VERSION
KEYWORDS    .
SOURCE      Synthetic plasmid
  ORGANISM  Synthetic plasmid
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 6342)
  AUTHORS   Glass,D.S. and Riedel-Kruse,I.H.
  TITLE     A synthetic bacterial cell-cell adhesion toolbox for programming
            multicellular morphologies and patterns
  JOURNAL   Cell (2018) In press
REFERENCE   2  (bases 1 to 6342)
  AUTHORS   Glass,D.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2018) Bioengineering, Stanford University, 318
            Campus Drive, Clark Center E350, Stanford, CA 94305, USA
COMMENT     Bankit Comment: ALT EMAIL:dsglass@gmail.com
            Bankit Comment: Vecscreen Comment:Submitter says that this sequence
            represents a Cloning Vector
            Bankit Comment: TOTAL # OF SEQS:83.
FEATURES             Location/Qualifiers
     source          1..6342
                     /organism="Synthetic plasmid"
                     /mol_type="other DNA"
                     /db_xref="taxon:1440148"
     misc_feature    1..1210
                     /label=I0500
     misc_feature    complement(1..1079)
                     /label=AraC
     misc_feature    complement(1043..1114)
                     /label=PC
     misc_feature    1080..1210
                     /label=pBAD
     misc_feature    1219..1230
                     /label=BBa_J04500
     misc_feature    1219..1230
                     /label=RBS (BBa_B0034)
     CDS             1237..3204
                     /codon_start=1
                     /transl_table=11
                     /product="Neae_removedRestrictionsites"
                     /translation="MITHGCYTRTRHKHKLKKTLIMLSAGLGLFFYVNQNSFANGENY
                     FKLGSDSKLLTHDSYQNRLFYTLKTGETVADLSKSQDINLSTIWSLNKHLYSSESEMM
                     KAAPGQQIILPLKKLPFEYSALPLLGSAPLVAAGGVAGHTNKLTKMSPDVTKSNMTDD
                     KALNYAAQQAASLGSQLQSRSLNGDYAKDTALGIAGNQASSQLQAWLQHYGTAEVNLQ
                     SGNNFDGSSLDFLLPFYDSEKMLAFGQVGARYIDSRFTANLGAGQRFFLPANMLGYNV
                     FIDQDFSGDNTRLGIGGEYWRDYFKSSVNGYFRMSGWHESYNKKDYDERPANGFDIRF
                     NGYLPSYPALGAKLIYEQYYGDNVALFNSDKLQSNPGAATVGVNYTPIPLVTMGIDYR
                     HGTGNENDLLYSMQFRYQFDKSWSQQIEPQYVNELRTLSGSRYDLVQRNNNIILEYKK
                     QDILSLNIPHDINGTEHSTQKIQLIVKSKYGLDRIVWDDSALRSQGGQIQHSGSQSAQ
                     DYQAILPAYVQGGSNIYKVTARAYDRNGNSSNNVQLTITVLSNGQVVDQVGVTDFTAD
                     KTSAKADNADTITYTATVKKNGVAQANVPVSFNIVSGTATLGANSAKTDANGKATVTL
                     KSSTPGQVVVSAKTAEMTSALNASAVIFFDGA"
     CDS             3211..3588
                     /codon_start=1
                     /transl_table=11
                     /product="Nanobody against EPEA tag"
                     /translation="-
                     GQLVESGGGSVQAGGSLRLSCAASGIDSSSYCMGWFRQRPGKEREGVARINGLGGVKT
                     AYADSVKDRFTISRDNAENTVYLQMNSLKPEDTAIYYCAAKFSPGYCGGSWSNFGYWG
                     QGTQVTVSS"
     misc_feature    3589..3594
                     /label=Stop codons
     misc_feature    3595..3615
                     /label=biobrick suffix
     misc_feature    3616..3687
                     /label=his operon terminator
     misc_feature    complement(3751..3768)
                     /label=VR primer site
     CDS             complement(3831..4646)
                     /codon_start=1
                     /transl_table=11
                     /product="KanR"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     misc_feature    complement(4978..5802)
                     /label=p15A ORI
     misc_feature    6203..6223
                     /label=VF2 primer site
     misc_feature    6321..6342
                     /label=biobrick prefix
BASE COUNT     1706 a   1497 c   1483 g   1656 t
ORIGIN      
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagaa agaggagaaa tactagatga ttactcatgg ttgttatacc
     1261 cggacccggc acaagcataa gctaaaaaaa acattgatta tgcttagtgc tggtttagga
     1321 ttgttttttt atgttaatca gaactcattt gcaaatggtg aaaattattt taaattgggt
     1381 tcggattcaa aactgttaac tcatgatagc tatcagaatc gcctttttta tacgttgaaa
     1441 actggtgaaa ctgttgccga tctttctaaa tcgcaagata ttaatttatc gacgatttgg
     1501 tcgttgaata agcatttata cagttctgaa agcgaaatga tgaaggccgc gcctggtcag
     1561 cagatcattt tgccactcaa aaaacttccc tttgaataca gtgcactacc acttttaggt
     1621 tcggcacctc ttgttgctgc gggtggtgtt gctggtcaca cgaataaact gactaaaatg
     1681 tccccggacg tgaccaaaag caacatgacc gatgacaagg cattaaatta tgcggcacaa
     1741 caggcggcga gtctcggtag ccagcttcag tcgcgatctc tgaacggcga ttacgcgaaa
     1801 gataccgctc ttggtatcgc tggtaaccag gcttcgtcac agttgcaggc ctggttacaa
     1861 cattatggaa cggcagaggt taatctgcaa agtggtaata actttgacgg tagttcactg
     1921 gacttcttat taccgttcta tgattccgaa aaaatgctgg catttggtca ggtcggagcg
     1981 cgttacattg actcccgctt tacggcaaat ttaggtgcgg gtcagcgttt tttccttcct
     2041 gcaaacatgt tgggctataa cgtcttcatt gatcaggatt tttctggtga taatacccgt
     2101 ttaggtattg gtggcgaata ctggcgagac tatttcaaaa gtagcgttaa cggctatttc
     2161 cgcatgagcg gctggcatga gtcatacaat aagaaagact atgatgagcg cccagcaaat
     2221 ggcttcgata tccgttttaa tggctatcta ccgtcatatc cggcattagg cgccaagctg
     2281 atatatgagc agtattatgg tgataatgtt gctttgttta attctgataa gctgcaatcg
     2341 aatcctggtg cggcgaccgt tggtgtaaac tatactccga ttcctctggt gacgatgggg
     2401 atcgattacc gtcatggtac gggtaatgaa aatgatctcc tttactcaat gcagttccgt
     2461 tatcagtttg ataaatcgtg gtctcagcaa attgaaccac agtatgttaa cgagttaaga
     2521 acattatcag gcagccgtta cgatctggtt cagcgtaata acaatattat tctggagtac
     2581 aagaagcagg atattctttc tctgaatatt ccgcatgata ttaatggtac tgaacacagt
     2641 acgcagaaga ttcagttgat cgttaagagc aaatacggtc tggatcgtat cgtctgggat
     2701 gatagtgcat tacgcagtca gggcggtcag attcagcata gcggaagcca aagcgcacaa
     2761 gactaccagg ctattttgcc tgcttatgtg caaggtggca gcaatattta taaagtgacg
     2821 gctcgcgcct atgaccgtaa tggcaatagc tctaacaatg tacagcttac tattaccgtt
     2881 ctgtcgaatg gtcaagttgt cgaccaggtt ggggtaacgg actttacggc ggataagact
     2941 tcggctaaag cggataacgc cgataccatt acttataccg cgacggtgaa aaagaatggg
     3001 gtagctcagg ctaatgtccc tgtttcattt aatattgttt caggaactgc aactcttggg
     3061 gcaaatagtg ccaaaacgga tgctaacggt aaggcaaccg taacgttgaa gtcgagtacg
     3121 ccaggacagg tcgtcgtgtc tgctaaaacc gcggagatga cttcagcact taatgccagt
     3181 gcggttatat tttttgatgg tgcgactaga caagggcagc ttgttgagag cgggggggga
     3241 agcgtacaag ccgggggatc gctgcgcttg tcgtgtgcag cttccggtat tgattccagc
     3301 tcatactgta tgggttggtt tcgtcagcgt ccgggaaagg agcgcgaggg tgtcgcacgt
     3361 atcaacgggt tgggcggcgt taaaacagct tacgctgact ctgttaaaga tcgtttcacc
     3421 attagtcgtg ataatgcaga aaatactgtt tatttgcaaa tgaactcctt gaaacccgaa
     3481 gatacagcta tttattattg tgctgccaag tttagtccag gttattgcgg aggatcgtgg
     3541 agcaatttcg gatactgggg tcaaggaacg caagtgaccg tatcttcgta ataatactag
     3601 tagcggccgc tgcagtccgg caaaaaaggg caaggtgtca ccaccctgcc ctttttcttt
     3661 aaaaccgaaa agattacttc gcgttatgca ggcttcctcg ctcactgact cgctgcgctc
     3721 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatct cgagtcccgt
     3781 caagtcagcg taatgctctg ccagtgttac aaccaattaa ccaattctga ttagaaaaac
     3841 tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt
     3901 tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca
     3961 agatcctggt atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc
     4021 ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt
     4081 gagaatggca aaagcttatg catttctttc cagacttgtt caacaggcca gccattacgc
     4141 tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg
     4201 agacgaaata cgcgatcgct gttaaaagga caattacaaa caggaatcga atgcaaccgg
     4261 cgcaggaaca ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat
     4321 acctggaatg ctgttttccc ggggatcgca gtggtgagta accatgcatc atcaggagta
     4381 cggataaaat gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc
     4441 atctcatctg taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc
     4501 gcatcgggct tcccatacaa tcgatagatt gtcgcacctg attgcccgac attatcgcga
     4561 gcccatttat acccatataa atcagcatcc atgttggaat ttaatcgcgg cctcgagcaa
     4621 gacgtttccc gttgaatatg gctcataaca ccccttgtat tactgtttat gtaagcagac
     4681 agttttattg ttcatgatga tatattttta tcttgtgcaa tgtaacatca gagattttga
     4741 gacacaacgt ggctttgttg aataaatcga acttttgctg agttgaagga tcagatcacg
     4801 catcttcccg acaacgcaga ccgttccgtg gcaaagcaaa agttcaaaat caccaactgg
     4861 tccacctaca acaaagctct catcaaccgt ggctccctca ctttctggct ggatgatggg
     4921 gcgattcagg cctggtatga gtcagcaaca ccttcttcac gaggcagacc tcagcgctag
     4981 cggagtgtat actggcttac tatgttggca ctgatgaggg tgtcagtgaa gtgcttcatg
     5041 tggcaggaga aaaaaggctg caccggtgcg tcagcagaat atgtgataca ggatatattc
     5101 cgcttcctcg ctcactgact cgctacgctc ggtcgttcga ctgcggcgag cggaaatggc
     5161 ttacgaacgg ggcggagatt tcctggaaga tgccaggaag atacttaaca gggaagtgag
     5221 agggccgcgg caaagccgtt tttccatagg ctccgccccc ctgacaagca tcacgaaatc
     5281 tgacgctcaa atcagtggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     5341 ctggcggctc cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt gtcattccgc
     5401 tgttatggcc gcgtttgtct cattccacgc ctgacactca gttccgggta ggcagttcgc
     5461 tccaagctgg actgtatgca cgaacccccc gttcagtccg accgctgcgc cttatccggt
     5521 aactatcgtc ttgagtccaa cccggaaaga catgcaaaag caccactggc agcagccact
     5581 ggtaattgat ttagaggagt tagtcttgaa gtcatgcgcc ggttaaggct aaactgaaag
     5641 gacaagtttt ggtgactgcg ctcctccaag ccagttacct cggttcaaag agttggtagc
     5701 tcagagaacc ttcgaaaaac cgccctgcaa ggcggttttt tcgttttcag agcaagagat
     5761 tacgcgcaga ccaaaacgat ctcaagaaga tcatcttatt aaggggtctg acgctcagtg
     5821 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     5881 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     5941 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     6001 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     6061 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     6121 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     6181 ctccatccag tctattccat ggtgccacct gacgtctaag aaaccattat tatcatgaca
     6241 ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa atccttagct
     6301 ttcgctaagg atgatttctg gaattcgcgg ccgcttctag ag
//
LOCUS       pDSG310                 5976 bp    DNA     circular SYN 14-JUN-2018
DEFINITION  Synthetic plasmid.
ACCESSION   pDSG310
VERSION
KEYWORDS    .
SOURCE      Synthetic plasmid
  ORGANISM  Synthetic plasmid
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 5976)
  AUTHORS   Glass,D.S. and Riedel-Kruse,I.H.
  TITLE     A synthetic bacterial cell-cell adhesion toolbox for programming
            multicellular morphologies and patterns
  JOURNAL   Cell (2018) In press
REFERENCE   2  (bases 1 to 5976)
  AUTHORS   Glass,D.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2018) Bioengineering, Stanford University, 318
            Campus Drive, Clark Center E350, Stanford, CA 94305, USA
COMMENT     Bankit Comment: ALT EMAIL:dsglass@gmail.com
            Bankit Comment: Vecscreen Comment:Submitter says that this sequence
            represents a Cloning Vector
            Bankit Comment: TOTAL # OF SEQS:83.
FEATURES             Location/Qualifiers
     source          1..5976
                     /organism="Synthetic plasmid"
                     /mol_type="other DNA"
                     /db_xref="taxon:1440148"
     misc_feature    1..1210
                     /label=I0500
     misc_feature    complement(1..1079)
                     /label=AraC
     misc_feature    complement(1043..1114)
                     /label=PC
     misc_feature    1080..1210
                     /label=pBAD
     misc_feature    1219..1230
                     /label=BBa_J04500
     misc_feature    1219..1230
                     /label=RBS (BBa_B0034)
     CDS             1237..3204
                     /codon_start=1
                     /transl_table=11
                     /product="Neae_removedRestrictionsites"
                     /translation="MITHGCYTRTRHKHKLKKTLIMLSAGLGLFFYVNQNSFANGENY
                     FKLGSDSKLLTHDSYQNRLFYTLKTGETVADLSKSQDINLSTIWSLNKHLYSSESEMM
                     KAAPGQQIILPLKKLPFEYSALPLLGSAPLVAAGGVAGHTNKLTKMSPDVTKSNMTDD
                     KALNYAAQQAASLGSQLQSRSLNGDYAKDTALGIAGNQASSQLQAWLQHYGTAEVNLQ
                     SGNNFDGSSLDFLLPFYDSEKMLAFGQVGARYIDSRFTANLGAGQRFFLPANMLGYNV
                     FIDQDFSGDNTRLGIGGEYWRDYFKSSVNGYFRMSGWHESYNKKDYDERPANGFDIRF
                     NGYLPSYPALGAKLIYEQYYGDNVALFNSDKLQSNPGAATVGVNYTPIPLVTMGIDYR
                     HGTGNENDLLYSMQFRYQFDKSWSQQIEPQYVNELRTLSGSRYDLVQRNNNIILEYKK
                     QDILSLNIPHDINGTEHSTQKIQLIVKSKYGLDRIVWDDSALRSQGGQIQHSGSQSAQ
                     DYQAILPAYVQGGSNIYKVTARAYDRNGNSSNNVQLTITVLSNGQVVDQVGVTDFTAD
                     KTSAKADNADTITYTATVKKNGVAQANVPVSFNIVSGTATLGANSAKTDANGKATVTL
                     KSSTPGQVVVSAKTAEMTSALNASAVIFFDGA"
     CDS             3211..3222
                     /codon_start=1
                     /transl_table=11
                     /product="EPEA peptide (C-termial of alpha synuclein)"
                     /translation="-PEA"
     misc_feature    3223..3228
                     /label=Stop codons
     misc_feature    3229..3249
                     /label=biobrick suffix
     misc_feature    3250..3321
                     /label=his operon terminator
     misc_feature    complement(3385..3402)
                     /label=VR primer site
     CDS             complement(3465..4280)
                     /codon_start=1
                     /transl_table=11
                     /product="KanR"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     misc_feature    complement(4612..5436)
                     /label=p15A ORI
     misc_feature    5837..5857
                     /label=VF2 primer site
     misc_feature    5955..5976
                     /label=biobrick prefix
BASE COUNT     1622 a   1425 c   1372 g   1557 t
ORIGIN      
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagaa agaggagaaa tactagatga ttactcatgg ttgttatacc
     1261 cggacccggc acaagcataa gctaaaaaaa acattgatta tgcttagtgc tggtttagga
     1321 ttgttttttt atgttaatca gaactcattt gcaaatggtg aaaattattt taaattgggt
     1381 tcggattcaa aactgttaac tcatgatagc tatcagaatc gcctttttta tacgttgaaa
     1441 actggtgaaa ctgttgccga tctttctaaa tcgcaagata ttaatttatc gacgatttgg
     1501 tcgttgaata agcatttata cagttctgaa agcgaaatga tgaaggccgc gcctggtcag
     1561 cagatcattt tgccactcaa aaaacttccc tttgaataca gtgcactacc acttttaggt
     1621 tcggcacctc ttgttgctgc gggtggtgtt gctggtcaca cgaataaact gactaaaatg
     1681 tccccggacg tgaccaaaag caacatgacc gatgacaagg cattaaatta tgcggcacaa
     1741 caggcggcga gtctcggtag ccagcttcag tcgcgatctc tgaacggcga ttacgcgaaa
     1801 gataccgctc ttggtatcgc tggtaaccag gcttcgtcac agttgcaggc ctggttacaa
     1861 cattatggaa cggcagaggt taatctgcaa agtggtaata actttgacgg tagttcactg
     1921 gacttcttat taccgttcta tgattccgaa aaaatgctgg catttggtca ggtcggagcg
     1981 cgttacattg actcccgctt tacggcaaat ttaggtgcgg gtcagcgttt tttccttcct
     2041 gcaaacatgt tgggctataa cgtcttcatt gatcaggatt tttctggtga taatacccgt
     2101 ttaggtattg gtggcgaata ctggcgagac tatttcaaaa gtagcgttaa cggctatttc
     2161 cgcatgagcg gctggcatga gtcatacaat aagaaagact atgatgagcg cccagcaaat
     2221 ggcttcgata tccgttttaa tggctatcta ccgtcatatc cggcattagg cgccaagctg
     2281 atatatgagc agtattatgg tgataatgtt gctttgttta attctgataa gctgcaatcg
     2341 aatcctggtg cggcgaccgt tggtgtaaac tatactccga ttcctctggt gacgatgggg
     2401 atcgattacc gtcatggtac gggtaatgaa aatgatctcc tttactcaat gcagttccgt
     2461 tatcagtttg ataaatcgtg gtctcagcaa attgaaccac agtatgttaa cgagttaaga
     2521 acattatcag gcagccgtta cgatctggtt cagcgtaata acaatattat tctggagtac
     2581 aagaagcagg atattctttc tctgaatatt ccgcatgata ttaatggtac tgaacacagt
     2641 acgcagaaga ttcagttgat cgttaagagc aaatacggtc tggatcgtat cgtctgggat
     2701 gatagtgcat tacgcagtca gggcggtcag attcagcata gcggaagcca aagcgcacaa
     2761 gactaccagg ctattttgcc tgcttatgtg caaggtggca gcaatattta taaagtgacg
     2821 gctcgcgcct atgaccgtaa tggcaatagc tctaacaatg tacagcttac tattaccgtt
     2881 ctgtcgaatg gtcaagttgt cgaccaggtt ggggtaacgg actttacggc ggataagact
     2941 tcggctaaag cggataacgc cgataccatt acttataccg cgacggtgaa aaagaatggg
     3001 gtagctcagg ctaatgtccc tgtttcattt aatattgttt caggaactgc aactcttggg
     3061 gcaaatagtg ccaaaacgga tgctaacggt aaggcaaccg taacgttgaa gtcgagtacg
     3121 ccaggacagg tcgtcgtgtc tgctaaaacc gcggagatga cttcagcact taatgccagt
     3181 gcggttatat tttttgatgg tgcgactaga gaaccagagg cttaataata ctagtagcgg
     3241 ccgctgcagt ccggcaaaaa agggcaaggt gtcaccaccc tgcccttttt ctttaaaacc
     3301 gaaaagatta cttcgcgtta tgcaggcttc ctcgctcact gactcgctgc gctcggtcgt
     3361 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atctcgagtc ccgtcaagtc
     3421 agcgtaatgc tctgccagtg ttacaaccaa ttaaccaatt ctgattagaa aaactcatcg
     3481 agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa
     3541 agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc
     3601 tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg
     3661 tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat
     3721 ggcaaaagct tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca
     3781 tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg agcgagacga
     3841 aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg
     3901 aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc taatacctgg
     3961 aatgctgttt tcccggggat cgcagtggtg agtaaccatg catcatcagg agtacggata
     4021 aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct gaccatctca
     4081 tctgtaacat cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatcg
     4141 ggcttcccat acaatcgata gattgtcgca cctgattgcc cgacattatc gcgagcccat
     4201 ttatacccat ataaatcagc atccatgttg gaatttaatc gcggcctcga gcaagacgtt
     4261 tcccgttgaa tatggctcat aacacccctt gtattactgt ttatgtaagc agacagtttt
     4321 attgttcatg atgatatatt tttatcttgt gcaatgtaac atcagagatt ttgagacaca
     4381 acgtggcttt gttgaataaa tcgaactttt gctgagttga aggatcagat cacgcatctt
     4441 cccgacaacg cagaccgttc cgtggcaaag caaaagttca aaatcaccaa ctggtccacc
     4501 tacaacaaag ctctcatcaa ccgtggctcc ctcactttct ggctggatga tggggcgatt
     4561 caggcctggt atgagtcagc aacaccttct tcacgaggca gacctcagcg ctagcggagt
     4621 gtatactggc ttactatgtt ggcactgatg agggtgtcag tgaagtgctt catgtggcag
     4681 gagaaaaaag gctgcaccgg tgcgtcagca gaatatgtga tacaggatat attccgcttc
     4741 ctcgctcact gactcgctac gctcggtcgt tcgactgcgg cgagcggaaa tggcttacga
     4801 acggggcgga gatttcctgg aagatgccag gaagatactt aacagggaag tgagagggcc
     4861 gcggcaaagc cgtttttcca taggctccgc ccccctgaca agcatcacga aatctgacgc
     4921 tcaaatcagt ggtggcgaaa cccgacagga ctataaagat accaggcgtt tcccctggcg
     4981 gctccctcgt gcgctctcct gttcctgcct ttcggtttac cggtgtcatt ccgctgttat
     5041 ggccgcgttt gtctcattcc acgcctgaca ctcagttccg ggtaggcagt tcgctccaag
     5101 ctggactgta tgcacgaacc ccccgttcag tccgaccgct gcgccttatc cggtaactat
     5161 cgtcttgagt ccaacccgga aagacatgca aaagcaccac tggcagcagc cactggtaat
     5221 tgatttagag gagttagtct tgaagtcatg cgccggttaa ggctaaactg aaaggacaag
     5281 ttttggtgac tgcgctcctc caagccagtt acctcggttc aaagagttgg tagctcagag
     5341 aaccttcgaa aaaccgccct gcaaggcggt tttttcgttt tcagagcaag agattacgcg
     5401 cagaccaaaa cgatctcaag aagatcatct tattaagggg tctgacgctc agtggaacga
     5461 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     5521 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     5581 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     5641 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     5701 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     5761 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     5821 ccagtctatt ccatggtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     5881 tataaaaata ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt agctttcgct
     5941 aaggatgatt tctggaattc gcggccgctt ctagag
//
LOCUS       pDSG263                 4131 bp    DNA     circular SYN 14-JUN-2018
DEFINITION  Synthetic plasmid.
ACCESSION   pDSG263
VERSION
KEYWORDS    .
SOURCE      Synthetic plasmid
  ORGANISM  Synthetic plasmid
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 4131)
  AUTHORS   Glass,D.S. and Riedel-Kruse,I.H.
  TITLE     A synthetic bacterial cell-cell adhesion toolbox for programming
            multicellular morphologies and patterns
  JOURNAL   Cell (2018) In press
REFERENCE   2  (bases 1 to 4131)
  AUTHORS   Glass,D.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2018) Bioengineering, Stanford University, 318
            Campus Drive, Clark Center E350, Stanford, CA 94305, USA
COMMENT     Bankit Comment: ALT EMAIL:dsglass@gmail.com
            Bankit Comment: Vecscreen Comment:Submitter says that this sequence
            represents a Cloning Vector
            Bankit Comment: TOTAL # OF SEQS:83.
FEATURES             Location/Qualifiers
     source          1..4131
                     /organism="Synthetic plasmid"
                     /mol_type="other DNA"
                     /db_xref="taxon:1440148"
     misc_feature    1..49
                     /label=pLambda (BBa_R0051)
     misc_feature    58..69
                     /label=RBS (BBa_B0034)
     CDS             76..792
                     /codon_start=1
                     /transl_table=11
                     /product="superfolder GFP orf"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKD
                     DGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNG
                     IKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHM
                     VLLEFVTAAGITHGMDELYK"
     misc_feature    793..813
                     /label=biobrick suffix
     misc_feature    814..887
                     /label=terminator
     CDS             complement(1176..2126)
                     /codon_start=1
                     /transl_table=11
                     /product="repA"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRK
                     ERTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAK
                     FSSEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHK
                     ANIEISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTL
                     IFQVELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTS
                     FEAKFLSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     misc_feature    complement(2130..2142)
                     /label=RBS
     regulatory      complement(2147..2166)
                     /regulatory_class="promoter"
                     /label=repA promoter
     CDS             complement(3012..3872)
                     /codon_start=1
                     /transl_table=11
                     /product="AmpR"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     misc_feature    complement(3873..3951)
                     /label=Promoter/RBS for AmpR
     misc_feature    4110..4131
                     /label=BioBricks prefix
BASE COUNT     1096 a    919 c    890 g   1226 t
ORIGIN      
        1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagaaa
       61 gaggagaaat actagatgcg taaaggcgaa gagctgttca ctggtgtcgt ccctattctg
      121 gtggaactgg atggtgatgt caacggtcat aagttttccg tgcgtggcga gggtgaaggt
      181 gacgcaacta atggtaaact gacgctgaag ttcatctgta ctactggtaa actgccggta
      241 ccttggccga ctctggtaac gacgctgact tatggtgttc agtgctttgc tcgttatccg
      301 gaccatatga agcagcatga cttcttcaag tccgccatgc cggaaggcta tgtgcaggaa
      361 cgcacgattt cctttaagga tgacggcacg tacaaaacgc gtgcggaagt gaaatttgaa
      421 ggcgataccc tggtaaaccg cattgagctg aaaggcattg actttaaaga agacggcaat
      481 atcctgggcc ataagctgga atacaatttt aacagccaca atgtttacat caccgccgat
      541 aaacaaaaaa atggcattaa agcgaatttt aaaattcgcc acaacgtgga ggatggcagc
      601 gtgcagctgg ctgatcacta ccagcaaaac actccaatcg gtgatggtcc tgttctgctg
      661 ccagacaatc actatctgag cacgcaaagc gttctgtcta aagatccgaa cgagaaacgc
      721 gatcatatgg ttctgctgga gttcgtaacc gcagcgggca tcacgcatgg tatggatgaa
      781 ctgtacaaat gatactagta gcggccgctg cagtccggca aaaaaacggg caaggtgtca
      841 ccaccctgcc ctttttcttt aaaaccgaaa agattacttc gcgttatgca ggcttcctcg
      901 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
      961 gcggtaatct cgaggttaca ttgtcgatct gttcatggtg aacagcttta aatgcaccaa
     1021 aaactcgtaa aagctctgat gtatctatct tttttacacc gttttcatct gtgcatatgg
     1081 acagttttcc ctttgatatc taacggtgaa cagttgttct acttttgttt gttagtcttg
     1141 atgcttcact gatagataca agagccataa gaacctcaga tccttccgta tttagccagt
     1201 atgttctcta gtgtggttcg ttgtttttgc gtgagccatg agaacgaacc attgagatca
     1261 tgcttacttt gcatgtcact caaaaatttt gcctcaaaac tggtgagctg aatttttgca
     1321 gttaaagcat cgtgtagtgt ttttcttagt ccgttacgta ggtaggaatc tgatgtaatg
     1381 gttgttggta ttttgtcacc attcattttt atctggttgt tctcaagttc ggttacgaga
     1441 tccatttgtc tatctagttc aacttggaaa atcaacgtat cagtcgggcg gcctcgctta
     1501 tcaaccacca atttcatatt gctgtaagtg tttaaatctt tacttattgg tttcaaaacc
     1561 cattggttaa gccttttaaa ctcatggtag ttattttcaa gcattaacat gaacttaaat
     1621 tcatcaaggc taatctctat atttgccttg tgagttttct tttgtgttag ttcttttaat
     1681 aaccactcat aaatcctcat agagtatttg ttttcaaaag acttaacatg ttccagatta
     1741 tattttatga atttttttaa ctggaaaaga taaggcaata tctcttcact aaaaactaat
     1801 tctaattttt cgcttgagaa cttggcatag tttgtccact ggaaaatctc aaagccttta
     1861 accaaaggat tcctgatttc cacagttctc gtcatcagct ctctggttgc tttagctaat
     1921 acaccataag cattttccct actgatgttc atcatctgag cgtattggtt ataagtgaac
     1981 gataccgtcc gttctttcct tgtagggttt tcaatcgtgg ggttgagtag tgccacacag
     2041 cataaaatta gcttggtttc atgctccgtt aagtcatagc gactaatcgc tagttcattt
     2101 gctttgaaaa caactaattc agacatacat ctcaattggt ctaggtgatt ttaatcacta
     2161 taccaattga gatgggctag tcaatgataa ttacatgtcc ttttcctttg agttgtgggt
     2221 atctgtaaat tctgctagac ctttgctgga aaacttgtaa attctgctag accctctgta
     2281 aattccgcta gacctttgtg tgtttttttt gtttatattc aagtggttat aatttataga
     2341 ataaagaaag aataaaaaaa gataaaaaga atagatccca gccctgtgta taactcacta
     2401 ctttagtcag ttccgcagta ttacaaaagg atgtcgcaaa cgctgtttgc tcctctacaa
     2461 aacagacctt aaaaccctaa aggcttaagt agcaccctcg caagctcggg caaatcgctg
     2521 aatattcctt ttgtctccga ccatcaggca cctgagtcgc tgtctttttc gtgacattca
     2581 gttcgctgcg ctcacggctc tggcagtgaa tgggggtaaa tggcactaca ggcgcctttt
     2641 atggattcat gcaaggaaac tacccataat acaagaaaag cccgtcacgg gcttctcagg
     2701 gcgttttatg gcgggtctgc tatgtggtgc tatctgactt tttgctgttc agcagttcct
     2761 gccctctgat tttccagtct gaccacttcg gattatcccg tgacaggtca ttcagactgg
     2821 ctaatgcacc cagtaaggca gcggtatcat caacaggctt acccgtctta ctgtccctag
     2881 tgcttggatt ctcaccaata aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca
     2941 gatggagttc tgaggtcatt actggatcta tcaacaggag tccaagcgag ctcgtaaact
     3001 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     3061 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     3121 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     3181 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     3241 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     3301 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     3361 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     3421 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     3481 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     3541 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     3601 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     3661 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     3721 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     3781 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     3841 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     3901 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     3961 caaatagggg ttccgcgcac atttcccatg gtgccacctg acgtctaaga aaccattatt
     4021 atcatgacat taacctataa aaataggcgt atcacgaggc agaatttcag ataaaaaaaa
     4081 tccttagctt tcgctaagga tgatttctgg aattcgcggc cgcttctaga g
//
LOCUS       pDSG262                 3560 bp    DNA     circular SYN 14-JUN-2018
DEFINITION  Synthetic plasmid.
ACCESSION   pDSG262
VERSION
KEYWORDS    .
SOURCE      Synthetic plasmid
  ORGANISM  Synthetic plasmid
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Glass,D.S. and Riedel-Kruse,I.H.
  TITLE     A synthetic bacterial cell-cell adhesion toolbox for programming
            multicellular morphologies and patterns
  JOURNAL   Cell (2018) In press
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Glass,D.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2018) Bioengineering, Stanford University, 318
            Campus Drive, Clark Center E350, Stanford, CA 94305, USA
COMMENT     Bankit Comment: ALT EMAIL:dsglass@gmail.com
            Bankit Comment: Vecscreen Comment:Submitter says that this sequence
            represents a Cloning Vector
            Bankit Comment: TOTAL # OF SEQS:83.
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Synthetic plasmid"
                     /mol_type="other DNA"
                     /db_xref="taxon:1440148"
     misc_feature    complement(1..3541)
                     /label=biobrick suffix
     primer_bind     complement(1..3541)
                     /label=Biobricks suffix
     misc_feature    2..75
                     /label=his operon terminator
     prim_transcript 2..75
                     /label=E.coli his operon terminator
     stem_loop       10..44
                     /label=terminator stem loop
     primer_bind     complement(134..156)
                     /label=VR primer site
     misc_feature    complement(139..156)
                     /label=VR primer site
     CDS             complement(219..1034)
                     /codon_start=1
                     /transl_table=11
                     /product="KanR"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     misc_feature    complement(1366..2190)
                     /label=p15A ORI
     misc_feature    2591..2611
                     /label=VF2 primer site
     stem_loop       2680..2699
                     /label=terminator stem loop(1)
     misc_feature    2709..2730
                     /label=biobrick prefix
     misc_feature    2709..2728
                     /label=BioBricks Prefix
     misc_feature    2731..2779
                     /label=pLambda (BBa_R0051)
     misc_feature    2788..2799
                     /label=RBS (BBa_B0034)
     misc_feature    2806..3540
                     /label=mRuby2 cds
BASE COUNT      973 a    863 c    837 g    887 t
ORIGIN      
        1 gtccggcaaa aaaacgggca aggtgtcacc accctgccct ttttctttaa aaccgaaaag
       61 attacttcgc gttatgcagg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
      121 gcggcgagcg gtatcagctc actcaaaggc ggtaatctcg agtcccgtca agtcagcgta
      181 atgctctgcc agtgttacaa ccaattaacc aattctgatt agaaaaactc atcgagcatc
      241 aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt
      301 ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat
      361 cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa
      421 ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa
      481 agcttatgca tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa
      541 tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg
      601 cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact
      661 gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct
      721 gttttcccgg ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc
      781 ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta
      841 acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc
      901 ccatacaatc gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac
      961 ccatataaat cagcatccat gttggaattt aatcgcggcc tcgagcaaga cgtttcccgt
     1021 tgaatatggc tcataacacc ccttgtatta ctgtttatgt aagcagacag ttttattgtt
     1081 catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga cacaacgtgg
     1141 ctttgttgaa taaatcgaac ttttgctgag ttgaaggatc agatcacgca tcttcccgac
     1201 aacgcagacc gttccgtggc aaagcaaaag ttcaaaatca ccaactggtc cacctacaac
     1261 aaagctctca tcaaccgtgg ctccctcact ttctggctgg atgatggggc gattcaggcc
     1321 tggtatgagt cagcaacacc ttcttcacga ggcagacctc agcgctagcg gagtgtatac
     1381 tggcttacta tgttggcact gatgagggtg tcagtgaagt gcttcatgtg gcaggagaaa
     1441 aaaggctgca ccggtgcgtc agcagaatat gtgatacagg atatattccg cttcctcgct
     1501 cactgactcg ctacgctcgg tcgttcgact gcggcgagcg gaaatggctt acgaacgggg
     1561 cggagatttc ctggaagatg ccaggaagat acttaacagg gaagtgagag ggccgcggca
     1621 aagccgtttt tccataggct ccgcccccct gacaagcatc acgaaatctg acgctcaaat
     1681 cagtggtggc gaaacccgac aggactataa agataccagg cgtttcccct ggcggctccc
     1741 tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc
     1801 gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac
     1861 tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt
     1921 gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt
     1981 agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg
     2041 tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt
     2101 cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc
     2161 aaaacgatct caagaagatc atcttattaa ggggtctgac gctcagtgga acgaaaactc
     2221 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     2281 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     2341 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     2401 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     2461 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     2521 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     2581 tattccatgg tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     2641 aataggcgta tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat
     2701 gatttctgga attcgcggcc gcttctagag taacaccgtg cgtgttgact attttacctc
     2761 tggcggtgat aatggttgct actagagaaa gaggagaaat actagatggt gtctaagggc
     2821 gaagagctga tcaaggaaaa tatgcgtatg aaggtggtca tggaaggttc ggtcaacggc
     2881 caccaattca aatgcacagg tgaaggagaa ggcaatccgt acatgggaac tcaaaccatg
     2941 aggatcaaag tcatcgaggg aggacccctg ccatttgcct ttgacattct tgccacgtcg
     3001 ttcatgtatg gcagccgtac ttttatcaag tacccgaaag gcattcctga tttctttaaa
     3061 cagtcctttc ctgagggttt tacttgggaa agagttacga gatacgaaga tggtggagtc
     3121 gtcaccgtca tgcaggacac cagccttgag gatggctgtc tcgtttacca cgtccaagtc
     3181 agaggggtaa actttccctc caatggtccc gtgatgcaga agaagaccaa gggttgggag
     3241 cctaatacag agatgatgta tccagcagat ggtggtctga ggggatacac tcatatggca
     3301 ctgaaagttg atggtggtgg ccatctgtct tgctctttcg taacaactta caggtcaaaa
     3361 aagaccgtcg ggaacatcaa gatgcccggt atccatgccg ttgatcaccg cctggaaagg
     3421 ttagaggaaa gtgacaatga aatgttcgta gtacaacgcg aacacgcagt tgccaagttc
     3481 gccgggcttg gtggtgggat ggacgagctg tacaagcatc accaccacca tcactaatga
     3541 tactagtagc ggccgctgca
//
LOCUS       pDSG264                 3543 bp    DNA     circular SYN 14-JUN-2018
DEFINITION  Synthetic plasmid.
ACCESSION   pDSG264
VERSION
KEYWORDS    .
SOURCE      Synthetic plasmid
  ORGANISM  Synthetic plasmid
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Glass,D.S. and Riedel-Kruse,I.H.
  TITLE     A synthetic bacterial cell-cell adhesion toolbox for programming
            multicellular morphologies and patterns
  JOURNAL   Cell (2018) In press
REFERENCE   2  (bases 1 to 3543)
  AUTHORS   Glass,D.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2018) Bioengineering, Stanford University, 318
            Campus Drive, Clark Center E350, Stanford, CA 94305, USA
COMMENT     Bankit Comment: ALT EMAIL:dsglass@gmail.com
            Bankit Comment: Vecscreen Comment:Submitter says that this sequence
            represents a Cloning Vector
            Bankit Comment: TOTAL # OF SEQS:83.
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Synthetic plasmid"
                     /mol_type="other DNA"
                     /db_xref="taxon:1440148"
     misc_feature    2..73
                     /label=his operon terminator
     prim_transcript 2..73
                     /label=E.coli his operon terminator
     stem_loop       10..42
                     /label=terminator stem loop
     primer_bind     complement(132..154)
                     /label=VR primer site(1)
     misc_feature    complement(137..154)
                     /label=VR primer site
     CDS             complement(217..1032)
                     /codon_start=1
                     /transl_table=11
                     /product="KanR"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     misc_feature    complement(1364..2188)
                     /label=p15A ORI
     misc_feature    2589..2609
                     /label=VF2 primer site
     stem_loop       2678..2697
                     /label=terminator stem loop(1)
     misc_feature    2707..2728
                     /label=biobrick prefix
     misc_feature    2712..2819
                     /label=insert from pXJ147_pLambda-mRuby2_pSC101.xdna
     misc_feature    2729..2777
                     /label=pLambda (BBa_R0051)
     misc_feature    2786..2797
                     /label=RBS (BBa_B0034)
     misc_feature    2804..3523
                     /label=Superfolder GFP
     misc_feature    3524..3543
                     /label=biobrick suffix
BASE COUNT      971 a    861 c    823 g    888 t
ORIGIN      
        1 gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt ttctttaaaa ccgaaaagat
       61 tacttcgcgt tatgcaggct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
      121 ggcgagcggt atcagctcac tcaaaggcgg taatctcgag tcccgtcaag tcagcgtaat
      181 gctctgccag tgttacaacc aattaaccaa ttctgattag aaaaactcat cgagcatcaa
      241 atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt
      301 ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat cctggtatcg
      361 gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat
      421 aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag
      481 cttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaaatc
      541 actcgcatca accaaaccgt tattcattcg tgattgcgcc tgagcgagac gaaatacgcg
      601 atcgctgtta aaaggacaat tacaaacagg aatcgaatgc aaccggcgca ggaacactgc
      661 cagcgcatca acaatatttt cacctgaatc aggatattct tctaatacct ggaatgctgt
      721 tttcccgggg atcgcagtgg tgagtaacca tgcatcatca ggagtacgga taaaatgctt
      781 gatggtcgga agaggcataa attccgtcag ccagtttagt ctgaccatct catctgtaac
      841 atcattggca acgctacctt tgccatgttt cagaaacaac tctggcgcat cgggcttccc
      901 atacaatcga tagattgtcg cacctgattg cccgacatta tcgcgagccc atttataccc
      961 atataaatca gcatccatgt tggaatttaa tcgcggcctc gagcaagacg tttcccgttg
     1021 aatatggctc ataacacccc ttgtattact gtttatgtaa gcagacagtt ttattgttca
     1081 tgatgatata tttttatctt gtgcaatgta acatcagaga ttttgagaca caacgtggct
     1141 ttgttgaata aatcgaactt ttgctgagtt gaaggatcag atcacgcatc ttcccgacaa
     1201 cgcagaccgt tccgtggcaa agcaaaagtt caaaatcacc aactggtcca cctacaacaa
     1261 agctctcatc aaccgtggct ccctcacttt ctggctggat gatggggcga ttcaggcctg
     1321 gtatgagtca gcaacacctt cttcacgagg cagacctcag cgctagcgga gtgtatactg
     1381 gcttactatg ttggcactga tgagggtgtc agtgaagtgc ttcatgtggc aggagaaaaa
     1441 aggctgcacc ggtgcgtcag cagaatatgt gatacaggat atattccgct tcctcgctca
     1501 ctgactcgct acgctcggtc gttcgactgc ggcgagcgga aatggcttac gaacggggcg
     1561 gagatttcct ggaagatgcc aggaagatac ttaacaggga agtgagaggg ccgcggcaaa
     1621 gccgtttttc cataggctcc gcccccctga caagcatcac gaaatctgac gctcaaatca
     1681 gtggtggcga aacccgacag gactataaag ataccaggcg tttcccctgg cggctccctc
     1741 gtgcgctctc ctgttcctgc ctttcggttt accggtgtca ttccgctgtt atggccgcgt
     1801 ttgtctcatt ccacgcctga cactcagttc cgggtaggca gttcgctcca agctggactg
     1861 tatgcacgaa ccccccgttc agtccgaccg ctgcgcctta tccggtaact atcgtcttga
     1921 gtccaacccg gaaagacatg caaaagcacc actggcagca gccactggta attgatttag
     1981 aggagttagt cttgaagtca tgcgccggtt aaggctaaac tgaaaggaca agttttggtg
     2041 actgcgctcc tccaagccag ttacctcggt tcaaagagtt ggtagctcag agaaccttcg
     2101 aaaaaccgcc ctgcaaggcg gttttttcgt tttcagagca agagattacg cgcagaccaa
     2161 aacgatctca agaagatcat cttattaagg ggtctgacgc tcagtggaac gaaaactcac
     2221 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     2281 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     2341 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     2401 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     2461 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     2521 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     2581 ttccatggtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     2641 taggcgtatc acgaggcaga atttcagata aaaaaaatcc ttagctttcg ctaaggatga
     2701 tttctggaat tcgcggccgc ttctagagta acaccgtgcg tgttgactat tttacctctg
     2761 gcggtgataa tggttgctac tagagaaaga ggagaaatac tagatgcgta aaggcgaaga
     2821 gctgttcact ggtgtcgtcc ctattctggt ggaactggat ggtgatgtca acggtcataa
     2881 gttttccgtg cgtggcgagg gtgaaggtga cgcaactaat ggtaaactga cgctgaagtt
     2941 catctgtact actggtaaac tgccggtacc ttggccgact ctggtaacga cgctgactta
     3001 tggtgttcag tgctttgctc gttatccgga ccatatgaag cagcatgact tcttcaagtc
     3061 cgccatgccg gaaggctatg tgcaggaacg cacgatttcc tttaaggatg acggcacgta
     3121 caaaacgcgt gcggaagtga aatttgaagg cgataccctg gtaaaccgca ttgagctgaa
     3181 aggcattgac tttaaagaag acggcaatat cctgggccat aagctggaat acaattttaa
     3241 cagccacaat gtttacatca ccgccgataa acaaaaaaat ggcattaaag cgaattttaa
     3301 aattcgccac aacgtggagg atggcagcgt gcagctggct gatcactacc agcaaaacac
     3361 tccaatcggt gatggtcctg ttctgctgcc agacaatcac tatctgagca cgcaaagcgt
     3421 tctgtctaaa gatccgaacg agaaacgcga tcatatggtt ctgctggagt tcgtaaccgc
     3481 agcgggcatc acgcatggta tggatgaact gtacaaatga tgatactagt agcggccgct
     3541 gca
//
LOCUS       pDSG6                   2964 bp    DNA     circular SYN 14-JUN-2018
DEFINITION  Synthetic plasmid.
ACCESSION   pDSG6
VERSION
KEYWORDS    .
SOURCE      Synthetic plasmid
  ORGANISM  Synthetic plasmid
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Glass,D.S. and Riedel-Kruse,I.H.
  TITLE     A synthetic bacterial cell-cell adhesion toolbox for programming
            multicellular morphologies and patterns
  JOURNAL   Cell (2018) In press
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Glass,D.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2018) Bioengineering, Stanford University, 318
            Campus Drive, Clark Center E350, Stanford, CA 94305, USA
COMMENT     Bankit Comment: ALT EMAIL:dsglass@gmail.com
            Bankit Comment: Vecscreen Comment:Submitter says that this sequence
            represents a Cloning Vector
            Bankit Comment: TOTAL # OF SEQS:83.
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Synthetic plasmid"
                     /mol_type="other DNA"
                     /db_xref="taxon:1440148"
     misc_feature    1..35
                     /label=BBa_J23101
     misc_feature    45..56
                     /label=RBS
     CDS             62..778
                     /codon_start=1
                     /transl_table=11
                     /product="Cerulean"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKN
                     GIKANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK"
     misc_feature    896..915
                     /label=BioBrick suffix
     regulatory      916..987
                     /regulatory_class="terminator"
                     /label=terminator
     rep_origin      1155..1769
                     /label=rep (pMB1)
     regulatory      1936..2041
                     /regulatory_class="terminator"
                     /label=terminator(1)
     CDS             complement(2057..2713)
                     /codon_start=1
                     /transl_table=11
                     /product="CmR"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     misc_feature    2945..2964
                     /label=BioBrick prefix
BASE COUNT      805 a    769 c    731 g    659 t
ORIGIN      
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gtataaagag gagaaattca
       61 gatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
      121 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
      181 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
      241 cctcgtgacc accctgagct ggggcgtgca gtgcttcgcc cgctaccccg accacatgaa
      301 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
      361 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
      421 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
      481 caagctggag tacaacgcca tccacggcaa cgtctatatc accgccgaca agcagaagaa
      541 cggcatcaag gccaacttcg gcctgaactg caacatcgag gacggcagcg tgcagctcgc
      601 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
      661 ctacctgagc acccagtcca agctgagcaa agaccccaac gagaagcgcg atcacatggt
      721 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
      781 aactagtatt aaagaggaga aattcaagat ggtgagcaag gacgagctgt acaagtaaac
      841 tagtattaaa gaggagaaat tcaagatggt gagcaagggc gagctgtaca agtaaactag
      901 tagcggccgc tgcagtccgg caaaaaaggg caaggtgtca ccaccctgcc ctttttcttt
      961 aaaaccgaaa agattacttc gcgttatgca ggcttcctcg ctcactgact cgctgcgctc
     1021 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     1081 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     1141 ccgtaaaaag gccgcgttgc tggcgttttt ccacaggctc cgcccccctg acgagcatca
     1201 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     1261 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     1321 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     1381 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     1441 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     1501 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     1561 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     1621 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     1681 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     1741 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     1801 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     1861 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     1921 tgacagctcg aggcttggat tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct
     1981 gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcga
     2041 gctcgatatc aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa
     2101 gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca
     2161 tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt
     2221 tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga
     2281 cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg
     2341 ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga
     2401 gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc
     2461 atatcaccag ctcaccgtct ttcattgcca tacgaaattc cggatgagca ttcatcaggc
     2521 gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta
     2581 aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa
     2641 atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga
     2701 tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc
     2761 ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc ccgatcaact
     2821 cgagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     2881 cgtatcacga ggcagaattt cagataaaaa aaatccttag ctttcgctaa ggatgatttc
     2941 tggaattcgc ggccgcttct agag
//
LOCUS       pDSG7                   2964 bp    DNA     circular SYN 14-JUN-2018
DEFINITION  Synthetic plasmid.
ACCESSION   pDSG7
VERSION
KEYWORDS    .
SOURCE      Synthetic plasmid
  ORGANISM  Synthetic plasmid
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Glass,D.S. and Riedel-Kruse,I.H.
  TITLE     A synthetic bacterial cell-cell adhesion toolbox for programming
            multicellular morphologies and patterns
  JOURNAL   Cell (2018) In press
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Glass,D.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2018) Bioengineering, Stanford University, 318
            Campus Drive, Clark Center E350, Stanford, CA 94305, USA
COMMENT     Bankit Comment: ALT EMAIL:dsglass@gmail.com
            Bankit Comment: Vecscreen Comment:Submitter says that this sequence
            represents a Cloning Vector
            Bankit Comment: TOTAL # OF SEQS:83.
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Synthetic plasmid"
                     /mol_type="other DNA"
                     /db_xref="taxon:1440148"
     misc_feature    1..35
                     /label=BBa_J23101
     misc_feature    45..56
                     /label=RBS
     misc_feature    62..781
                     /label=Venus
     misc_feature    896..915
                     /label=BioBrick suffix
     regulatory      916..987
                     /regulatory_class="terminator"
                     /label=terminator
     rep_origin      1155..1769
                     /label=rep (pMB1)
     regulatory      1936..2041
                     /regulatory_class="terminator"
                     /label=terminator(1)
     CDS             complement(2057..2713)
                     /codon_start=1
                     /transl_table=11
                     /product="CmR"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     misc_feature    2945..2964
                     /label=BioBrick prefix
BASE COUNT      810 a    770 c    726 g    658 t
ORIGIN      
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gtataaagag gagaaattca
       61 gatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
      121 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
      181 cggcaagctg accctgaagc tgatctgcac caccggcaag ctgcccgtgc cctggcccac
      241 cctcgtgacc accctgggct acggcctaca gtgcttcgcc cgctaccccg accacatgaa
      301 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
      361 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
      421 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
      481 caagctggag tacaactaca acagccacaa cgtctatatc accgccgaca agcagaagaa
      541 cggcatcaag gccaacttca agatccgcca caacatcgag gacggcggcg tgcagctcgc
      601 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
      661 ctacctgagc taccagtcca agctgagcaa agaccccaac gagaagcgcg atcacatggt
      721 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
      781 aactagtatt aaagaggaga aattcaagat ggtgagcaag gacgagctgt acaagtaaac
      841 tagtattaaa gaggagaaat tcaagatggt gagcaagggc gagctgtaca agtaaactag
      901 tagcggccgc tgcagtccgg caaaaaaggg caaggtgtca ccaccctgcc ctttttcttt
      961 aaaaccgaaa agattacttc gcgttatgca ggcttcctcg ctcactgact cgctgcgctc
     1021 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     1081 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     1141 ccgtaaaaag gccgcgttgc tggcgttttt ccacaggctc cgcccccctg acgagcatca
     1201 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     1261 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     1321 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     1381 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     1441 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     1501 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     1561 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     1621 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     1681 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     1741 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     1801 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     1861 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     1921 tgacagctcg aggcttggat tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct
     1981 gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcga
     2041 gctcgatatc aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa
     2101 gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca
     2161 tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt
     2221 tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga
     2281 cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg
     2341 ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga
     2401 gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc
     2461 atatcaccag ctcaccgtct ttcattgcca tacgaaattc cggatgagca ttcatcaggc
     2521 gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta
     2581 aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa
     2641 atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga
     2701 tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc
     2761 ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc ccgatcaact
     2821 cgagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     2881 cgtatcacga ggcagaattt cagataaaaa aaatccttag ctttcgctaa ggatgatttc
     2941 tggaattcgc ggccgcttct agag
//
LOCUS       pDSG261                 4149 bp    DNA     circular SYN 14-JUN-2018
DEFINITION  Synthetic plasmid.
ACCESSION   pDSG261
VERSION
KEYWORDS    .
SOURCE      Synthetic plasmid
  ORGANISM  Synthetic plasmid
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Glass,D.S. and Riedel-Kruse,I.H.
  TITLE     A synthetic bacterial cell-cell adhesion toolbox for programming
            multicellular morphologies and patterns
  JOURNAL   Cell (2018) In press
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   Glass,D.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2018) Bioengineering, Stanford University, 318
            Campus Drive, Clark Center E350, Stanford, CA 94305, USA
COMMENT     Bankit Comment: ALT EMAIL:dsglass@gmail.com
            Bankit Comment: Vecscreen Comment:Submitter says that this sequence
            represents a Cloning Vector
            Bankit Comment: TOTAL # OF SEQS:83.
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Synthetic plasmid"
                     /mol_type="other DNA"
                     /db_xref="taxon:1440148"
     misc_feature    1..49
                     /label=pLambda (BBa_R0051)
     misc_feature    58..69
                     /label=RBS (BBa_B0034)
     misc_feature    76..810
                     /label=mRuby2
     misc_feature    811..831
                     /label=biobrick suffix
     misc_feature    832..905
                     /label=terminator
     CDS             complement(1194..2144)
                     /codon_start=1
                     /transl_table=11
                     /product="repA"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRK
                     ERTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAK
                     FSSEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHK
                     ANIEISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTL
                     IFQVELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTS
                     FEAKFLSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     misc_feature    complement(2148..2160)
                     /label=RBS
     regulatory      complement(2165..2184)
                     /regulatory_class="promoter"
                     /label=repA promoter
     CDS             complement(3030..3890)
                     /codon_start=1
                     /transl_table=11
                     /product="AmpR"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     misc_feature    complement(3891..3969)
                     /label=Promoter/RBS for AmpR
     misc_feature    4128..4149
                     /label=BioBricks prefix
BASE COUNT     1098 a    920 c    905 g   1226 t
ORIGIN      
        1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagaaa
       61 gaggagaaat actagatggt gtctaagggc gaagagctga tcaaggaaaa tatgcgtatg
      121 aaggtggtca tggaaggttc ggtcaacggc caccaattca aatgcacagg tgaaggagaa
      181 ggcaatccgt acatgggaac tcaaaccatg aggatcaaag tcatcgaggg aggacccctg
      241 ccatttgcct ttgacattct tgccacgtcg ttcatgtatg gcagccgtac ttttatcaag
      301 tacccgaaag gcattcctga tttctttaaa cagtcctttc ctgagggttt tacttgggaa
      361 agagttacga gatacgaaga tggtggagtc gtcaccgtca tgcaggacac cagccttgag
      421 gatggctgtc tcgtttacca cgtccaagtc agaggggtaa actttccctc caatggtccc
      481 gtgatgcaga agaagaccaa gggttgggag cctaatacag agatgatgta tccagcagat
      541 ggtggtctga ggggatacac tcatatggca ctgaaagttg atggtggtgg ccatctgtct
      601 tgctctttcg taacaactta caggtcaaaa aagaccgtcg ggaacatcaa gatgcccggt
      661 atccatgccg ttgatcaccg cctggaaagg ttagaggaaa gtgacaatga aatgttcgta
      721 gtacaacgcg aacacgcagt tgccaagttc gccgggcttg gtggtgggat ggacgagctg
      781 tacaagcatc accaccacca tcactaatga tactagtagc ggccgctgca gtccggcaaa
      841 aaaacgggca aggtgtcacc accctgccct ttttctttaa aaccgaaaag attacttcgc
      901 gttatgcagg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
      961 gtatcagctc actcaaaggc ggtaatctcg aggttacatt gtcgatctgt tcatggtgaa
     1021 cagctttaaa tgcaccaaaa actcgtaaaa gctctgatgt atctatcttt tttacaccgt
     1081 tttcatctgt gcatatggac agttttccct ttgatatcta acggtgaaca gttgttctac
     1141 ttttgtttgt tagtcttgat gcttcactga tagatacaag agccataaga acctcagatc
     1201 cttccgtatt tagccagtat gttctctagt gtggttcgtt gtttttgcgt gagccatgag
     1261 aacgaaccat tgagatcatg cttactttgc atgtcactca aaaattttgc ctcaaaactg
     1321 gtgagctgaa tttttgcagt taaagcatcg tgtagtgttt ttcttagtcc gttacgtagg
     1381 taggaatctg atgtaatggt tgttggtatt ttgtcaccat tcatttttat ctggttgttc
     1441 tcaagttcgg ttacgagatc catttgtcta tctagttcaa cttggaaaat caacgtatca
     1501 gtcgggcggc ctcgcttatc aaccaccaat ttcatattgc tgtaagtgtt taaatcttta
     1561 cttattggtt tcaaaaccca ttggttaagc cttttaaact catggtagtt attttcaagc
     1621 attaacatga acttaaattc atcaaggcta atctctatat ttgccttgtg agttttcttt
     1681 tgtgttagtt cttttaataa ccactcataa atcctcatag agtatttgtt ttcaaaagac
     1741 ttaacatgtt ccagattata ttttatgaat ttttttaact ggaaaagata aggcaatatc
     1801 tcttcactaa aaactaattc taatttttcg cttgagaact tggcatagtt tgtccactgg
     1861 aaaatctcaa agcctttaac caaaggattc ctgatttcca cagttctcgt catcagctct
     1921 ctggttgctt tagctaatac accataagca ttttccctac tgatgttcat catctgagcg
     1981 tattggttat aagtgaacga taccgtccgt tctttccttg tagggttttc aatcgtgggg
     2041 ttgagtagtg ccacacagca taaaattagc ttggtttcat gctccgttaa gtcatagcga
     2101 ctaatcgcta gttcatttgc tttgaaaaca actaattcag acatacatct caattggtct
     2161 aggtgatttt aatcactata ccaattgaga tgggctagtc aatgataatt acatgtcctt
     2221 ttcctttgag ttgtgggtat ctgtaaattc tgctagacct ttgctggaaa acttgtaaat
     2281 tctgctagac cctctgtaaa ttccgctaga cctttgtgtg ttttttttgt ttatattcaa
     2341 gtggttataa tttatagaat aaagaaagaa taaaaaaaga taaaaagaat agatcccagc
     2401 cctgtgtata actcactact ttagtcagtt ccgcagtatt acaaaaggat gtcgcaaacg
     2461 ctgtttgctc ctctacaaaa cagaccttaa aaccctaaag gcttaagtag caccctcgca
     2521 agctcgggca aatcgctgaa tattcctttt gtctccgacc atcaggcacc tgagtcgctg
     2581 tctttttcgt gacattcagt tcgctgcgct cacggctctg gcagtgaatg ggggtaaatg
     2641 gcactacagg cgccttttat ggattcatgc aaggaaacta cccataatac aagaaaagcc
     2701 cgtcacgggc ttctcagggc gttttatggc gggtctgcta tgtggtgcta tctgactttt
     2761 tgctgttcag cagttcctgc cctctgattt tccagtctga ccacttcgga ttatcccgtg
     2821 acaggtcatt cagactggct aatgcaccca gtaaggcagc ggtatcatca acaggcttac
     2881 ccgtcttact gtccctagtg cttggattct caccaataaa aaacgcccgg cggcaaccga
     2941 gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc
     3001 caagcgagct cgtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     3061 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     3121 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     3181 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     3241 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     3301 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     3361 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     3421 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     3481 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     3541 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     3601 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     3661 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     3721 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     3781 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     3841 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     3901 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     3961 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttcccatggt gccacctgac
     4021 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggcag
     4081 aatttcagat aaaaaaaatc cttagctttc gctaaggatg atttctggaa ttcgcggccg
     4141 cttctagag
//
LOCUS       pDSG8                   2955 bp    DNA     circular SYN 14-JUN-2018
DEFINITION  Synthetic plasmid.
ACCESSION   pDSG8
VERSION
KEYWORDS    .
SOURCE      Synthetic plasmid
  ORGANISM  Synthetic plasmid
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Glass,D.S. and Riedel-Kruse,I.H.
  TITLE     A synthetic bacterial cell-cell adhesion toolbox for programming
            multicellular morphologies and patterns
  JOURNAL   Cell (2018) In press
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   Glass,D.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2018) Bioengineering, Stanford University, 318
            Campus Drive, Clark Center E350, Stanford, CA 94305, USA
COMMENT     Bankit Comment: ALT EMAIL:dsglass@gmail.com
            Bankit Comment: Vecscreen Comment:Submitter says that this sequence
            represents a Cloning Vector
            Bankit Comment: TOTAL # OF SEQS:83.
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Synthetic plasmid"
                     /mol_type="other DNA"
                     /db_xref="taxon:1440148"
     misc_feature    1..35
                     /label=BBa_J23101
     misc_feature    45..56
                     /label=RBS
     CDS             65..769
                     /codon_start=1
                     /transl_table=11
                     /product="mCherry"
                     /translation="MSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMN
                     FEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGA
                     LKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQY
                     ERAEGRHSTGGMDELYK"
     misc_feature    887..906
                     /label=BioBrick suffix
     regulatory      907..978
                     /regulatory_class="terminator"
                     /label=terminator
     rep_origin      1146..1760
                     /label=rep (pMB1)
     regulatory      1927..2032
                     /regulatory_class="terminator"
                     /label=terminator(1)
     CDS             complement(2048..2704)
                     /codon_start=1
                     /transl_table=11
                     /product="CmR"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     misc_feature    2936..2955
                     /label=BioBrick prefix
BASE COUNT      801 a    753 c    743 g    658 t
ORIGIN      
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gtataaagag gagaaattca
       61 gatggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa
      121 ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
      181 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc
      241 cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca
      301 ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg
      361 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga
      421 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt
      481 aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg
      541 cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc
      601 tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt
      661 caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga
      721 acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt aaactagtat
      781 taaagaggag aaattcaaga tggtgagcaa ggacgagctg tacaagtaaa ctagtattaa
      841 agaggagaaa ttcaagatgg tgagcaaggg cgagctgtac aagtaaacta gtagcggccg
      901 ctgcagtccg gcaaaaaagg gcaaggtgtc accaccctgc cctttttctt taaaaccgaa
      961 aagattactt cgcgttatgc aggcttcctc gctcactgac tcgctgcgct cggtcgttcg
     1021 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     1081 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     1141 ggccgcgttg ctggcgtttt tccacaggct ccgcccccct gacgagcatc acaaaaatcg
     1201 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     1261 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     1321 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     1381 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     1441 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     1501 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     1561 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     1621 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     1681 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     1741 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     1801 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     1861 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagctc
     1921 gaggcttgga ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc
     1981 cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagcg agctcgatat
     2041 caaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc
     2101 cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct
     2161 tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat
     2221 tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca
     2281 tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt
     2341 gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa
     2401 acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca
     2461 gctcaccgtc tttcattgcc atacgaaatt ccggatgagc attcatcagg cgggcaagaa
     2521 tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg
     2581 taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa
     2641 aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg atttttttct
     2701 ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg
     2761 atcttatttc attatggtga aagttggaac ctcttacgtg cccgatcaac tcgagtgcca
     2821 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     2881 aggcagaatt tcagataaaa aaaatcctta gctttcgcta aggatgattt ctggaattcg
     2941 cggccgcttc tagag
//
LOCUS       pDSG253                 6186 bp    DNA     circular SYN 14-JUN-2018
DEFINITION  Synthetic plasmid.
ACCESSION   pDSG253
VERSION
KEYWORDS    .
SOURCE      Synthetic plasmid
  ORGANISM  Synthetic plasmid
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 6186)
  AUTHORS   Glass,D.S. and Riedel-Kruse,I.H.
  TITLE     A synthetic bacterial cell-cell adhesion toolbox for programming
            multicellular morphologies and patterns
  JOURNAL   Cell (2018) In press
REFERENCE   2  (bases 1 to 6186)
  AUTHORS   Glass,D.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2018) Bioengineering, Stanford University, 318
            Campus Drive, Clark Center E350, Stanford, CA 94305, USA
COMMENT     Bankit Comment: ALT EMAIL:dsglass@gmail.com
            Bankit Comment: Vecscreen Comment:Submitter says that this sequence
            represents a Cloning Vector
            Bankit Comment: TOTAL # OF SEQS:83.
FEATURES             Location/Qualifiers
     source          1..6186
                     /organism="Synthetic plasmid"
                     /mol_type="other DNA"
                     /db_xref="taxon:1440148"
     misc_feature    1..73
                     /label=LacI promoter
     CDS             74..1165
                     /codon_start=1
                     /transl_table=11
                     /product="LacI"
                     /translation="MVNVKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAA
                     MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMV
                     ERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPIN
                     SIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAE
                     REGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGY
                     DDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLA
                     PNTQTASPRALADSLMQLARQVSRLESGQ"
     misc_feature    1166..1382
                     /label=pLac
     misc_feature    1383..3350
                     /label=intimin N-terminus
     misc_feature    3351..3389
                     /label=E-tag
     CDS             3393..3770
                     /codon_start=1
                     /transl_table=11
                     /product="N4-1_antiEPEA"
                     /translation="-
                     GQLVESGGGSVQAGGSLRLSCAASGIDSSSYCMGWFRQRPGKEREGVARINGLGGVKT
                     AYADSVKDRFTISRDNAENTVYLQMNSLKPEDTAIYYCAAKFSPGYCGGSWSNFGYWG
                     QGTQVTVSS"
     misc_feature    3780..3806
                     /label=myc tag
     CDS             4547..5206
                     /codon_start=1
                     /transl_table=11
                     /product="CmR"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     misc_feature    5463..6141
                     /label=rep (pMB1)
BASE COUNT     1537 a   1438 c   1577 g   1634 t
ORIGIN      
        1 acccgacacc atcgaatggc gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga
       61 gagtcaattc agggtggtga atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc
      121 cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa
      181 aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc
      241 acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct
      301 gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag
      361 cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa
      421 tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc
      481 cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca
      541 gacacccatc aacagtatta ttttctccca tgaagacggt acgcgactgg gcgtggagca
      601 tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc
      661 ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat
      721 agcggaacgg gaaggcgact ggagtgccat gtccggtttt caacaaacca tgcaaatgct
      781 gaatgagggc atcgttccca ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc
      841 aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gacatctcgg tagtgggata
      901 cgacgatacc gaagacagct catgttatat cccgccgtta accaccatca aacaggattt
      961 tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt
     1021 gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa
     1081 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     1141 ttcccgactg gaaagcgggc agtgagcggt acccgataaa agcggcttcc tgacaggagg
     1201 ccgttttgtt ttgcagccca cctcaacgca attaatgtga gttagctcac tcattaggca
     1261 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     1321 caatttcaca caggaaacag ctatgaccat gattacgaat ttctagataa cgagggcaaa
     1381 tcatgattac tcatggttgt tatacccgga cccggcacaa gcataagcta aaaaaaacat
     1441 tgattatgct tagtgctggt ttaggattgt ttttttatgt taatcagaat tcatttgcaa
     1501 atggtgaaaa ttattttaaa ttgggttcgg attcaaaact gttaactcat gatagctatc
     1561 agaatcgcct tttttatacg ttgaaaactg gtgaaactgt tgccgatctt tctaaatcgc
     1621 aagatattaa tttatcgacg atttggtcgt tgaataagca tttatacagt tctgaaagcg
     1681 aaatgatgaa ggccgcgcct ggtcagcaga tcattttgcc actcaaaaaa cttccctttg
     1741 aatacagtgc actaccactt ttaggttcgg cacctcttgt tgctgcaggt ggtgttgctg
     1801 gtcacacgaa taaactgact aaaatgtccc cggacgtgac caaaagcaac atgaccgatg
     1861 acaaggcatt aaattatgcg gcacaacagg cggcgagtct cggtagccag cttcagtcgc
     1921 gatctctgaa cggcgattac gcgaaagata ccgctcttgg tatcgctggt aaccaggctt
     1981 cgtcacagtt gcaggcctgg ttacaacatt atggaacggc agaggttaat ctgcagagtg
     2041 gtaataactt tgacggtagt tcactggact tcttattacc gttctatgat tccgaaaaaa
     2101 tgctggcatt tggtcaggtc ggagcgcgtt acattgactc ccgctttacg gcaaatttag
     2161 gtgcgggtca gcgttttttc cttcctgcaa acatgttggg ctataacgtc ttcattgatc
     2221 aggatttttc tggtgataat acccgtttag gtattggtgg cgaatactgg cgagactatt
     2281 tcaaaagtag cgttaacggc tatttccgca tgagcggctg gcatgagtca tacaataaga
     2341 aagactatga tgagcgccca gcaaatggct tcgatatccg ttttaatggc tatctaccgt
     2401 catatccggc attaggcgcc aagctgatat atgagcagta ttatggtgat aatgttgctt
     2461 tgtttaattc tgataagctg cagtcgaatc ctggtgcggc gaccgttggt gtaaactata
     2521 ctccgattcc tctggtgacg atggggatcg attaccgtca tggtacgggt aatgaaaatg
     2581 atctccttta ctcaatgcag ttccgttatc agtttgataa atcgtggtct cagcaaattg
     2641 aaccacagta tgttaacgag ttaagaacat tatcaggcag ccgttacgat ctggttcagc
     2701 gtaataacaa tattattctg gagtacaaga agcaggatat tctttctctg aatattccgc
     2761 atgatattaa tggtactgaa cacagtacgc agaagattca gttgatcgtt aagagcaaat
     2821 acggtctgga tcgtatcgtc tgggatgata gtgcattacg cagtcagggc ggtcagattc
     2881 agcatagcgg aagccaaagc gcacaagact accaggctat tttgcctgct tatgtgcaag
     2941 gtggcagcaa tatttataaa gtgacggctc gcgcctatga ccgtaatggc aatagctcta
     3001 acaatgtaca gcttactatt accgttctgt cgaatggtca agttgtcgac caggttgggg
     3061 taacggactt tacggcggat aagacttcgg ctaaagcgga taacgccgat accattactt
     3121 ataccgcgac ggtgaaaaag aatggggtag ctcaggctaa tgtccctgtt tcatttaata
     3181 ttgtttcagg aactgcaact cttggggcaa atagtgccaa aacggatgct aacggtaagg
     3241 caaccgtaac gttgaagtcg agtacgccag gacaggtcgt cgtgtctgct aaaaccgcgg
     3301 agatgacttc agcacttaat gccagtgcgg ttatattttt tgatggtgcg ccggtgccgt
     3361 atccggatcc gctggaaccg gcccagccgg cccaagggca gcttgttgag agcggggggg
     3421 gaagcgtaca agccggggga tcgctgcgct tgtcgtgtgc agcttccggt attgattcca
     3481 gctcatactg tatgggttgg tttcgtcagc gtccgggaaa ggagcgcgag ggtgtcgcac
     3541 gtatcaacgg gttgggcggc gttaaaacag cttacgctga ctctgttaaa gatcgtttca
     3601 ccattagtcg tgataatgca gaaaatactg tttatttgca aatgaactcc ttgaaacccg
     3661 aagatacagc tatttattat tgtgctgcca agtttagtcc aggttattgc ggaggatcgt
     3721 ggagcaattt cggatactgg ggtcaaggaa cgcaagtgac cgtatcttcg gcggccgccg
     3781 aacaaaaact catctcagaa gaggatgcag ctgcataaaa gcttgacctg tgaagtgaaa
     3841 aatggcgcac attgtgcgac attttttttg tctgccgttt accgctactg cgtcacggat
     3901 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     3961 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     4021 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg gcatcccttt agggttccga
     4081 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     4141 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     4201 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     4261 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     4321 tttaacgcga attttaacaa aatattaacg tttacaattt caggtggcac ttttcgggga
     4381 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     4441 atgtcgagac gttgggtgag gttccaactt tcaccataat gaaataagat cactaccggg
     4501 cgtatttttt gagttatcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat
     4561 cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt
     4621 tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt
     4681 aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg
     4741 cctgatgaat gctcatccgg agttccgtat ggcaatgaaa gacggtgagc tggtgatatg
     4801 ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct
     4861 ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc
     4921 gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt
     4981 ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa
     5041 cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat
     5101 gccgctggcg attcaggttc atcatgccgt ctgtgatggc ttccatgtcg gcagaatgct
     5161 taatgaatta caacagtact gcgatgagtg gcagggcggg gcgtaatttt tttaaggcag
     5221 ttattggtgc ccttaaacgc ctggtgctac gcctgaataa gtgataataa gcggatgaat
     5281 ggcagaaatt cgaaagcaaa ttcgacccgg tcgtcggttc agggcagggt cgttaaatag
     5341 ccgcttatgt ctattgctgg tttaccggtt tattgactac cggaagcagt gtgaccgtgt
     5401 gcttctcaaa tgcctgaggc cagtttgctc aggctctccc cgtggaggta ataattgctc
     5461 gacatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     5521 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     5581 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     5641 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     5701 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     5761 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     5821 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     5881 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     5941 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     6001 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     6061 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     6121 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     6181 cacatg
//
LOCUS       pDSG254                 5826 bp    DNA     circular SYN 14-JUN-2018
DEFINITION  Synthetic plasmid.
ACCESSION   pDSG254
VERSION
KEYWORDS    .
SOURCE      Synthetic plasmid
  ORGANISM  Synthetic plasmid
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 5826)
  AUTHORS   Glass,D.S. and Riedel-Kruse,I.H.
  TITLE     A synthetic bacterial cell-cell adhesion toolbox for programming
            multicellular morphologies and patterns
  JOURNAL   Cell (2018) In press
REFERENCE   2  (bases 1 to 5826)
  AUTHORS   Glass,D.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2018) Bioengineering, Stanford University, 318
            Campus Drive, Clark Center E350, Stanford, CA 94305, USA
COMMENT     Bankit Comment: ALT EMAIL:dsglass@gmail.com
            Bankit Comment: Vecscreen Comment:Submitter says that this sequence
            represents a Cloning Vector
            Bankit Comment: TOTAL # OF SEQS:83.
FEATURES             Location/Qualifiers
     source          1..5826
                     /organism="Synthetic plasmid"
                     /mol_type="other DNA"
                     /db_xref="taxon:1440148"
     misc_feature    1..73
                     /label=LacI promoter
     CDS             74..1165
                     /codon_start=1
                     /transl_table=11
                     /product="LacI"
                     /translation="MVNVKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAA
                     MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMV
                     ERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPIN
                     SIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAE
                     REGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGY
                     DDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLA
                     PNTQTASPRALADSLMQLARQVSRLESGQ"
     misc_feature    1166..1382
                     /label=pLac
     misc_feature    1383..3350
                     /label=intimin N-terminus
     misc_feature    3351..3389
                     /label=E-tag
     CDS             3393..3404
                     /codon_start=1
                     /transl_table=11
                     /product="EPEA peptide (C-termial of alpha synuclein)"
                     /translation="-PEA"
     misc_feature    3393..3404
                     /product="Neae fusion"
     misc_feature    3420..3446
                     /label=myc tag
     CDS             4187..4846
                     /codon_start=1
                     /transl_table=11
                     /product="CmR"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     misc_feature    5103..5781
                     /label=rep (pMB1)
BASE COUNT     1457 a   1366 c   1466 g   1537 t
ORIGIN      
        1 acccgacacc atcgaatggc gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga
       61 gagtcaattc agggtggtga atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc
      121 cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa
      181 aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc
      241 acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct
      301 gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag
      361 cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa
      421 tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc
      481 cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca
      541 gacacccatc aacagtatta ttttctccca tgaagacggt acgcgactgg gcgtggagca
      601 tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc
      661 ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat
      721 agcggaacgg gaaggcgact ggagtgccat gtccggtttt caacaaacca tgcaaatgct
      781 gaatgagggc atcgttccca ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc
      841 aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gacatctcgg tagtgggata
      901 cgacgatacc gaagacagct catgttatat cccgccgtta accaccatca aacaggattt
      961 tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt
     1021 gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa
     1081 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     1141 ttcccgactg gaaagcgggc agtgagcggt acccgataaa agcggcttcc tgacaggagg
     1201 ccgttttgtt ttgcagccca cctcaacgca attaatgtga gttagctcac tcattaggca
     1261 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     1321 caatttcaca caggaaacag ctatgaccat gattacgaat ttctagataa cgagggcaaa
     1381 tcatgattac tcatggttgt tatacccgga cccggcacaa gcataagcta aaaaaaacat
     1441 tgattatgct tagtgctggt ttaggattgt ttttttatgt taatcagaat tcatttgcaa
     1501 atggtgaaaa ttattttaaa ttgggttcgg attcaaaact gttaactcat gatagctatc
     1561 agaatcgcct tttttatacg ttgaaaactg gtgaaactgt tgccgatctt tctaaatcgc
     1621 aagatattaa tttatcgacg atttggtcgt tgaataagca tttatacagt tctgaaagcg
     1681 aaatgatgaa ggccgcgcct ggtcagcaga tcattttgcc actcaaaaaa cttccctttg
     1741 aatacagtgc actaccactt ttaggttcgg cacctcttgt tgctgcaggt ggtgttgctg
     1801 gtcacacgaa taaactgact aaaatgtccc cggacgtgac caaaagcaac atgaccgatg
     1861 acaaggcatt aaattatgcg gcacaacagg cggcgagtct cggtagccag cttcagtcgc
     1921 gatctctgaa cggcgattac gcgaaagata ccgctcttgg tatcgctggt aaccaggctt
     1981 cgtcacagtt gcaggcctgg ttacaacatt atggaacggc agaggttaat ctgcagagtg
     2041 gtaataactt tgacggtagt tcactggact tcttattacc gttctatgat tccgaaaaaa
     2101 tgctggcatt tggtcaggtc ggagcgcgtt acattgactc ccgctttacg gcaaatttag
     2161 gtgcgggtca gcgttttttc cttcctgcaa acatgttggg ctataacgtc ttcattgatc
     2221 aggatttttc tggtgataat acccgtttag gtattggtgg cgaatactgg cgagactatt
     2281 tcaaaagtag cgttaacggc tatttccgca tgagcggctg gcatgagtca tacaataaga
     2341 aagactatga tgagcgccca gcaaatggct tcgatatccg ttttaatggc tatctaccgt
     2401 catatccggc attaggcgcc aagctgatat atgagcagta ttatggtgat aatgttgctt
     2461 tgtttaattc tgataagctg cagtcgaatc ctggtgcggc gaccgttggt gtaaactata
     2521 ctccgattcc tctggtgacg atggggatcg attaccgtca tggtacgggt aatgaaaatg
     2581 atctccttta ctcaatgcag ttccgttatc agtttgataa atcgtggtct cagcaaattg
     2641 aaccacagta tgttaacgag ttaagaacat tatcaggcag ccgttacgat ctggttcagc
     2701 gtaataacaa tattattctg gagtacaaga agcaggatat tctttctctg aatattccgc
     2761 atgatattaa tggtactgaa cacagtacgc agaagattca gttgatcgtt aagagcaaat
     2821 acggtctgga tcgtatcgtc tgggatgata gtgcattacg cagtcagggc ggtcagattc
     2881 agcatagcgg aagccaaagc gcacaagact accaggctat tttgcctgct tatgtgcaag
     2941 gtggcagcaa tatttataaa gtgacggctc gcgcctatga ccgtaatggc aatagctcta
     3001 acaatgtaca gcttactatt accgttctgt cgaatggtca agttgtcgac caggttgggg
     3061 taacggactt tacggcggat aagacttcgg ctaaagcgga taacgccgat accattactt
     3121 ataccgcgac ggtgaaaaag aatggggtag ctcaggctaa tgtccctgtt tcatttaata
     3181 ttgtttcagg aactgcaact cttggggcaa atagtgccaa aacggatgct aacggtaagg
     3241 caaccgtaac gttgaagtcg agtacgccag gacaggtcgt cgtgtctgct aaaaccgcgg
     3301 agatgacttc agcacttaat gccagtgcgg ttatattttt tgatggtgcg ccggtgccgt
     3361 atccggatcc gctggaaccg gcccagccgg ccgaaccaga ggcttaataa gcggccgccg
     3421 aacaaaaact catctcagaa gaggatgcag ctgcataaaa gcttgacctg tgaagtgaaa
     3481 aatggcgcac attgtgcgac attttttttg tctgccgttt accgctactg cgtcacggat
     3541 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     3601 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     3661 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg gcatcccttt agggttccga
     3721 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     3781 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     3841 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     3901 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     3961 tttaacgcga attttaacaa aatattaacg tttacaattt caggtggcac ttttcgggga
     4021 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     4081 atgtcgagac gttgggtgag gttccaactt tcaccataat gaaataagat cactaccggg
     4141 cgtatttttt gagttatcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat
     4201 cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt
     4261 tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt
     4321 aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg
     4381 cctgatgaat gctcatccgg agttccgtat ggcaatgaaa gacggtgagc tggtgatatg
     4441 ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct
     4501 ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc
     4561 gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt
     4621 ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa
     4681 cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat
     4741 gccgctggcg attcaggttc atcatgccgt ctgtgatggc ttccatgtcg gcagaatgct
     4801 taatgaatta caacagtact gcgatgagtg gcagggcggg gcgtaatttt tttaaggcag
     4861 ttattggtgc ccttaaacgc ctggtgctac gcctgaataa gtgataataa gcggatgaat
     4921 ggcagaaatt cgaaagcaaa ttcgacccgg tcgtcggttc agggcagggt cgttaaatag
     4981 ccgcttatgt ctattgctgg tttaccggtt tattgactac cggaagcagt gtgaccgtgt
     5041 gcttctcaaa tgcctgaggc cagtttgctc aggctctccc cgtggaggta ataattgctc
     5101 gacatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     5161 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     5221 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     5281 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     5341 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     5401 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     5461 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     5521 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     5581 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     5641 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     5701 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     5761 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     5821 cacatg
//
LOCUS       pDSG320                 6157 bp    DNA     circular SYN 14-JUN-2018
DEFINITION  Synthetic plasmid.
ACCESSION   pDSG320
VERSION
KEYWORDS    .
SOURCE      Synthetic plasmid
  ORGANISM  Synthetic plasmid
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 6157)
  AUTHORS   Glass,D.S. and Riedel-Kruse,I.H.
  TITLE     A synthetic bacterial cell-cell adhesion toolbox for programming
            multicellular morphologies and patterns
  JOURNAL   Cell (2018) In press
REFERENCE   2  (bases 1 to 6157)
  AUTHORS   Glass,D.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2018) Bioengineering, Stanford University, 318
            Campus Drive, Clark Center E350, Stanford, CA 94305, USA
COMMENT     Bankit Comment: ALT EMAIL:dsglass@gmail.com
            Bankit Comment: Vecscreen Comment:Submitter says that this sequence
            represents a Cloning Vector
            Bankit Comment: TOTAL # OF SEQS:83.
FEATURES             Location/Qualifiers
     source          1..6157
                     /organism="Synthetic plasmid"
                     /mol_type="other DNA"
                     /db_xref="taxon:1440148"
     misc_feature    1..689
                     /label=BBa_I732084
     misc_feature    1..36
                     /label=pLacIQ (BBa_I4032, 1nt deletion)
     misc_feature    45..56
                     /label=BBa_J04500(1)
     misc_feature    45..56
                     /label=RBS (BBa_B0034)
     CDS             63..686
                     /codon_start=1
                     /transl_table=11
                     /product="tetR ORF (BBa_I732080)"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     misc_feature    698..826
                     /label=Double terminator (BBa_B0015)
     misc_feature    835..896
                     /label=BBa_J13002
     misc_feature    835..888
                     /label=pTet (BBa_R0040)
     misc_feature    897..908
                     /label=BBa_J04500
     misc_feature    897..908
                     /label=RBS (BBa_B0034)(1)
     CDS             915..2882
                     /codon_start=1
                     /transl_table=11
                     /product="Neae_removedRestrictionsites"
                     /translation="MITHGCYTRTRHKHKLKKTLIMLSAGLGLFFYVNQNSFANGENY
                     FKLGSDSKLLTHDSYQNRLFYTLKTGETVADLSKSQDINLSTIWSLNKHLYSSESEMM
                     KAAPGQQIILPLKKLPFEYSALPLLGSAPLVAAGGVAGHTNKLTKMSPDVTKSNMTDD
                     KALNYAAQQAASLGSQLQSRSLNGDYAKDTALGIAGNQASSQLQAWLQHYGTAEVNLQ
                     SGNNFDGSSLDFLLPFYDSEKMLAFGQVGARYIDSRFTANLGAGQRFFLPANMLGYNV
                     FIDQDFSGDNTRLGIGGEYWRDYFKSSVNGYFRMSGWHESYNKKDYDERPANGFDIRF
                     NGYLPSYPALGAKLIYEQYYGDNVALFNSDKLQSNPGAATVGVNYTPIPLVTMGIDYR
                     HGTGNENDLLYSMQFRYQFDKSWSQQIEPQYVNELRTLSGSRYDLVQRNNNIILEYKK
                     QDILSLNIPHDINGTEHSTQKIQLIVKSKYGLDRIVWDDSALRSQGGQIQHSGSQSAQ
                     DYQAILPAYVQGGSNIYKVTARAYDRNGNSSNNVQLTITVLSNGQVVDQVGVTDFTAD
                     KTSAKADNADTITYTATVKKNGVAQANVPVSFNIVSGTATLGANSAKTDANGKATVTL
                     KSSTPGQVVVSAKTAEMTSALNASAVIFFDGA"
     CDS             2889..3266
                     /codon_start=1
                     /transl_table=11
                     /product="Nanobody against EPEA tag"
                     /translation="-
                     GQLVESGGGSVQAGGSLRLSCAASGIDSSSYCMGWFRQRPGKEREGVARINGLGGVKT
                     AYADSVKDRFTISRDNAENTVYLQMNSLKPEDTAIYYCAAKFSPGYCGGSWSNFGYWG
                     QGTQVTVSS"
     misc_feature    3267..3272
                     /label=Stop codons
     regulatory      3281..3409
                     /regulatory_class="terminator"
                     /label=terminator B0015
     misc_feature    3410..3430
                     /label=biobrick suffix
     misc_feature    3431..3502
                     /label=his operon terminator
     misc_feature    complement(3566..3583)
                     /label=VR primer site
     CDS             complement(3646..4461)
                     /codon_start=1
                     /transl_table=11
                     /product="KanR"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     misc_feature    complement(4793..5617)
                     /label=p15A ORI
     misc_feature    6018..6038
                     /label=VF2 primer site
     misc_feature    6136..6157
                     /label=biobrick prefix
BASE COUNT     1702 a   1366 c   1443 g   1646 t
ORIGIN      
        1 tggtgcaaaa cctttgcggt atggcatgat agcgcctact agagaaagag gagaaatact
       61 agatgtccag attagataaa agtaaagtga ttaacagcgc attagagctg cttaatgagg
      121 tcggaatcga aggtttaaca acccgtaaac tcgcccagaa gctaggtgta gagcagccta
      181 cattgtattg gcatgtaaaa aataagcggg ctttgctcga cgccttagcc attgagatgt
      241 tagataggca ccatactcac ttttgccctt tagaagggga aagctggcaa gattttttac
      301 gtaataacgc taaaagtttt agatgtgctt tactaagtca tcgcgatgga gcaaaagtac
      361 atttaggtac acggcctaca gaaaaacagt atgaaactct cgaaaatcaa ttagcctttt
      421 tatgccaaca aggtttttca ctagagaatg cattatatgc actcagcgct gtggggcatt
      481 ttactttagg ttgcgtattg gaagatcaag agcatcaagt cgctaaagaa gaaagggaaa
      541 cacctactac tgatagtatg ccgccattat tacgacaagc tatcgaatta tttgatcacc
      601 aaggtgcaga gccagccttc ttattcggcc ttgaattgat catatgcgga ttagaaaaac
      661 aacttaaatg tgaaagtggg tcctaataat actagagcca ggcatcaaat aaaacgaaag
      721 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
      781 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agagtcccta
      841 tcagtgatag agattgacat ccctatcagt gatagagata ctgagcacta ctagagaaag
      901 aggagaaata ctagatgatt actcatggtt gttatacccg gacccggcac aagcataagc
      961 taaaaaaaac attgattatg cttagtgctg gtttaggatt gtttttttat gttaatcaga
     1021 actcatttgc aaatggtgaa aattatttta aattgggttc ggattcaaaa ctgttaactc
     1081 atgatagcta tcagaatcgc cttttttata cgttgaaaac tggtgaaact gttgccgatc
     1141 tttctaaatc gcaagatatt aatttatcga cgatttggtc gttgaataag catttataca
     1201 gttctgaaag cgaaatgatg aaggccgcgc ctggtcagca gatcattttg ccactcaaaa
     1261 aacttccctt tgaatacagt gcactaccac ttttaggttc ggcacctctt gttgctgcgg
     1321 gtggtgttgc tggtcacacg aataaactga ctaaaatgtc cccggacgtg accaaaagca
     1381 acatgaccga tgacaaggca ttaaattatg cggcacaaca ggcggcgagt ctcggtagcc
     1441 agcttcagtc gcgatctctg aacggcgatt acgcgaaaga taccgctctt ggtatcgctg
     1501 gtaaccaggc ttcgtcacag ttgcaggcct ggttacaaca ttatggaacg gcagaggtta
     1561 atctgcaaag tggtaataac tttgacggta gttcactgga cttcttatta ccgttctatg
     1621 attccgaaaa aatgctggca tttggtcagg tcggagcgcg ttacattgac tcccgcttta
     1681 cggcaaattt aggtgcgggt cagcgttttt tccttcctgc aaacatgttg ggctataacg
     1741 tcttcattga tcaggatttt tctggtgata atacccgttt aggtattggt ggcgaatact
     1801 ggcgagacta tttcaaaagt agcgttaacg gctatttccg catgagcggc tggcatgagt
     1861 catacaataa gaaagactat gatgagcgcc cagcaaatgg cttcgatatc cgttttaatg
     1921 gctatctacc gtcatatccg gcattaggcg ccaagctgat atatgagcag tattatggtg
     1981 ataatgttgc tttgtttaat tctgataagc tgcaatcgaa tcctggtgcg gcgaccgttg
     2041 gtgtaaacta tactccgatt cctctggtga cgatggggat cgattaccgt catggtacgg
     2101 gtaatgaaaa tgatctcctt tactcaatgc agttccgtta tcagtttgat aaatcgtggt
     2161 ctcagcaaat tgaaccacag tatgttaacg agttaagaac attatcaggc agccgttacg
     2221 atctggttca gcgtaataac aatattattc tggagtacaa gaagcaggat attctttctc
     2281 tgaatattcc gcatgatatt aatggtactg aacacagtac gcagaagatt cagttgatcg
     2341 ttaagagcaa atacggtctg gatcgtatcg tctgggatga tagtgcatta cgcagtcagg
     2401 gcggtcagat tcagcatagc ggaagccaaa gcgcacaaga ctaccaggct attttgcctg
     2461 cttatgtgca aggtggcagc aatatttata aagtgacggc tcgcgcctat gaccgtaatg
     2521 gcaatagctc taacaatgta cagcttacta ttaccgttct gtcgaatggt caagttgtcg
     2581 accaggttgg ggtaacggac tttacggcgg ataagacttc ggctaaagcg gataacgccg
     2641 ataccattac ttataccgcg acggtgaaaa agaatggggt agctcaggct aatgtccctg
     2701 tttcatttaa tattgtttca ggaactgcaa ctcttggggc aaatagtgcc aaaacggatg
     2761 ctaacggtaa ggcaaccgta acgttgaagt cgagtacgcc aggacaggtc gtcgtgtctg
     2821 ctaaaaccgc ggagatgact tcagcactta atgccagtgc ggttatattt tttgatggtg
     2881 cgactagaca agggcagctt gttgagagcg gggggggaag cgtacaagcc gggggatcgc
     2941 tgcgcttgtc gtgtgcagct tccggtattg attccagctc atactgtatg ggttggtttc
     3001 gtcagcgtcc gggaaaggag cgcgagggtg tcgcacgtat caacgggttg ggcggcgtta
     3061 aaacagctta cgctgactct gttaaagatc gtttcaccat tagtcgtgat aatgcagaaa
     3121 atactgttta tttgcaaatg aactccttga aacccgaaga tacagctatt tattattgtg
     3181 ctgccaagtt tagtccaggt tattgcggag gatcgtggag caatttcgga tactggggtc
     3241 aaggaacgca agtgaccgta tcttcgtaat aatactagag ccaggcatca aataaaacga
     3301 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     3361 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat actagtagcg
     3421 gccgctgcag tccggcaaaa aagggcaagg tgtcaccacc ctgccctttt tctttaaaac
     3481 cgaaaagatt acttcgcgtt atgcaggctt cctcgctcac tgactcgctg cgctcggtcg
     3541 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatctcgagt cccgtcaagt
     3601 cagcgtaatg ctctgccagt gttacaacca attaaccaat tctgattaga aaaactcatc
     3661 gagcatcaaa tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa
     3721 aagccgtttc tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc
     3781 ctggtatcgg tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc
     3841 gtcaaaaata aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa
     3901 tggcaaaagc ttatgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc
     3961 atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct gagcgagacg
     4021 aaatacgcga tcgctgttaa aaggacaatt acaaacagga atcgaatgca accggcgcag
     4081 gaacactgcc agcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg
     4141 gaatgctgtt ttcccgggga tcgcagtggt gagtaaccat gcatcatcag gagtacggat
     4201 aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc cagtttagtc tgaccatctc
     4261 atctgtaaca tcattggcaa cgctaccttt gccatgtttc agaaacaact ctggcgcatc
     4321 gggcttccca tacaatcgat agattgtcgc acctgattgc ccgacattat cgcgagccca
     4381 tttataccca tataaatcag catccatgtt ggaatttaat cgcggcctcg agcaagacgt
     4441 ttcccgttga atatggctca taacacccct tgtattactg tttatgtaag cagacagttt
     4501 tattgttcat gatgatatat ttttatcttg tgcaatgtaa catcagagat tttgagacac
     4561 aacgtggctt tgttgaataa atcgaacttt tgctgagttg aaggatcaga tcacgcatct
     4621 tcccgacaac gcagaccgtt ccgtggcaaa gcaaaagttc aaaatcacca actggtccac
     4681 ctacaacaaa gctctcatca accgtggctc cctcactttc tggctggatg atggggcgat
     4741 tcaggcctgg tatgagtcag caacaccttc ttcacgaggc agacctcagc gctagcggag
     4801 tgtatactgg cttactatgt tggcactgat gagggtgtca gtgaagtgct tcatgtggca
     4861 ggagaaaaaa ggctgcaccg gtgcgtcagc agaatatgtg atacaggata tattccgctt
     4921 cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg gcgagcggaa atggcttacg
     4981 aacggggcgg agatttcctg gaagatgcca ggaagatact taacagggaa gtgagagggc
     5041 cgcggcaaag ccgtttttcc ataggctccg cccccctgac aagcatcacg aaatctgacg
     5101 ctcaaatcag tggtggcgaa acccgacagg actataaaga taccaggcgt ttcccctggc
     5161 ggctccctcg tgcgctctcc tgttcctgcc tttcggttta ccggtgtcat tccgctgtta
     5221 tggccgcgtt tgtctcattc cacgcctgac actcagttcc gggtaggcag ttcgctccaa
     5281 gctggactgt atgcacgaac cccccgttca gtccgaccgc tgcgccttat ccggtaacta
     5341 tcgtcttgag tccaacccgg aaagacatgc aaaagcacca ctggcagcag ccactggtaa
     5401 ttgatttaga ggagttagtc ttgaagtcat gcgccggtta aggctaaact gaaaggacaa
     5461 gttttggtga ctgcgctcct ccaagccagt tacctcggtt caaagagttg gtagctcaga
     5521 gaaccttcga aaaaccgccc tgcaaggcgg ttttttcgtt ttcagagcaa gagattacgc
     5581 gcagaccaaa acgatctcaa gaagatcatc ttattaaggg gtctgacgct cagtggaacg
     5641 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     5701 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     5761 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     5821 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     5881 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     5941 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     6001 tccagtctat tccatggtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     6061 ctataaaaat aggcgtatca cgaggcagaa tttcagataa aaaaaatcct tagctttcgc
     6121 taaggatgat ttctggaatt cgcggccgct tctagag
//
