LOCUS       pDSG323                 5635 bp    DNA     circular SYN 14-JUN-2018
DEFINITION  Synthetic plasmid.
ACCESSION   pDSG323
VERSION
KEYWORDS    .
SOURCE      Synthetic plasmid
  ORGANISM  Synthetic plasmid
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 5635)
  AUTHORS   Glass,D.S. and Riedel-Kruse,I.H.
  TITLE     A synthetic bacterial cell-cell adhesion toolbox for programming
            multicellular morphologies and patterns
  JOURNAL   Cell (2018) In press
REFERENCE   2  (bases 1 to 5635)
  AUTHORS   Glass,D.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2018) Bioengineering, Stanford University, 318
            Campus Drive, Clark Center E350, Stanford, CA 94305, USA
COMMENT     Bankit Comment: ALT EMAIL:dsglass@gmail.com
            Bankit Comment: Vecscreen Comment:Submitter says that this sequence
            represents a Cloning Vector
            Bankit Comment: TOTAL # OF SEQS:83.
FEATURES             Location/Qualifiers
     source          1..5635
                     /organism="Synthetic plasmid"
                     /mol_type="other DNA"
                     /db_xref="taxon:1440148"
     misc_feature    1..689
                     /label=BBa_I732084
     misc_feature    1..36
                     /label=pLacIQ (BBa_I4032, 1nt deletion)
     misc_feature    45..56
                     /label=BBa_J04500(1)
     misc_feature    45..56
                     /label=RBS (BBa_B0034)
     CDS             63..686
                     /codon_start=1
                     /transl_table=11
                     /product="tetR ORF (BBa_I732080)"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     misc_feature    698..826
                     /label=Double terminator (BBa_B0015)
     misc_feature    835..896
                     /label=BBa_J13002
     misc_feature    835..888
                     /label=pTet (BBa_R0040)
     misc_feature    897..908
                     /label=BBa_J04500
     misc_feature    897..908
                     /label=RBS (BBa_B0034)(1)
     CDS             915..2882
                     /codon_start=1
                     /transl_table=11
                     /product="Neae_removedRestrictionsites"
                     /translation="MITHGCYTRTRHKHKLKKTLIMLSAGLGLFFYVNQNSFANGENY
                     FKLGSDSKLLTHDSYQNRLFYTLKTGETVADLSKSQDINLSTIWSLNKHLYSSESEMM
                     KAAPGQQIILPLKKLPFEYSALPLLGSAPLVAAGGVAGHTNKLTKMSPDVTKSNMTDD
                     KALNYAAQQAASLGSQLQSRSLNGDYAKDTALGIAGNQASSQLQAWLQHYGTAEVNLQ
                     SGNNFDGSSLDFLLPFYDSEKMLAFGQVGARYIDSRFTANLGAGQRFFLPANMLGYNV
                     FIDQDFSGDNTRLGIGGEYWRDYFKSSVNGYFRMSGWHESYNKKDYDERPANGFDIRF
                     NGYLPSYPALGAKLIYEQYYGDNVALFNSDKLQSNPGAATVGVNYTPIPLVTMGIDYR
                     HGTGNENDLLYSMQFRYQFDKSWSQQIEPQYVNELRTLSGSRYDLVQRNNNIILEYKK
                     QDILSLNIPHDINGTEHSTQKIQLIVKSKYGLDRIVWDDSALRSQGGQIQHSGSQSAQ
                     DYQAILPAYVQGGSNIYKVTARAYDRNGNSSNNVQLTITVLSNGQVVDQVGVTDFTAD
                     KTSAKADNADTITYTATVKKNGVAQANVPVSFNIVSGTATLGANSAKTDANGKATVTL
                     KSSTPGQVVVSAKTAEMTSALNASAVIFFDGA"
     misc_feature    2883..2908
                     /label=RFC[23] biobrick suffix (partial)
     misc_feature    2909..2980
                     /label=his operon terminator
     misc_feature    complement(3044..3061)
                     /label=VR primer site
     CDS             complement(3124..3939)
                     /codon_start=1
                     /transl_table=11
                     /product="KanR"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     misc_feature    complement(4271..5095)
                     /label=p15A ORI
     misc_feature    5496..5516
                     /label=VF2 primer site
     misc_feature    5614..5635
                     /label=biobrick prefix
BASE COUNT     1580 a   1258 c   1294 g   1503 t
ORIGIN      
        1 tggtgcaaaa cctttgcggt atggcatgat agcgcctact agagaaagag gagaaatact
       61 agatgtccag attagataaa agtaaagtga ttaacagcgc attagagctg cttaatgagg
      121 tcggaatcga aggtttaaca acccgtaaac tcgcccagaa gctaggtgta gagcagccta
      181 cattgtattg gcatgtaaaa aataagcggg ctttgctcga cgccttagcc attgagatgt
      241 tagataggca ccatactcac ttttgccctt tagaagggga aagctggcaa gattttttac
      301 gtaataacgc taaaagtttt agatgtgctt tactaagtca tcgcgatgga gcaaaagtac
      361 atttaggtac acggcctaca gaaaaacagt atgaaactct cgaaaatcaa ttagcctttt
      421 tatgccaaca aggtttttca ctagagaatg cattatatgc actcagcgct gtggggcatt
      481 ttactttagg ttgcgtattg gaagatcaag agcatcaagt cgctaaagaa gaaagggaaa
      541 cacctactac tgatagtatg ccgccattat tacgacaagc tatcgaatta tttgatcacc
      601 aaggtgcaga gccagccttc ttattcggcc ttgaattgat catatgcgga ttagaaaaac
      661 aacttaaatg tgaaagtggg tcctaataat actagagcca ggcatcaaat aaaacgaaag
      721 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
      781 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agagtcccta
      841 tcagtgatag agattgacat ccctatcagt gatagagata ctgagcacta ctagagaaag
      901 aggagaaata ctagatgatt actcatggtt gttatacccg gacccggcac aagcataagc
      961 taaaaaaaac attgattatg cttagtgctg gtttaggatt gtttttttat gttaatcaga
     1021 actcatttgc aaatggtgaa aattatttta aattgggttc ggattcaaaa ctgttaactc
     1081 atgatagcta tcagaatcgc cttttttata cgttgaaaac tggtgaaact gttgccgatc
     1141 tttctaaatc gcaagatatt aatttatcga cgatttggtc gttgaataag catttataca
     1201 gttctgaaag cgaaatgatg aaggccgcgc ctggtcagca gatcattttg ccactcaaaa
     1261 aacttccctt tgaatacagt gcactaccac ttttaggttc ggcacctctt gttgctgcgg
     1321 gtggtgttgc tggtcacacg aataaactga ctaaaatgtc cccggacgtg accaaaagca
     1381 acatgaccga tgacaaggca ttaaattatg cggcacaaca ggcggcgagt ctcggtagcc
     1441 agcttcagtc gcgatctctg aacggcgatt acgcgaaaga taccgctctt ggtatcgctg
     1501 gtaaccaggc ttcgtcacag ttgcaggcct ggttacaaca ttatggaacg gcagaggtta
     1561 atctgcaaag tggtaataac tttgacggta gttcactgga cttcttatta ccgttctatg
     1621 attccgaaaa aatgctggca tttggtcagg tcggagcgcg ttacattgac tcccgcttta
     1681 cggcaaattt aggtgcgggt cagcgttttt tccttcctgc aaacatgttg ggctataacg
     1741 tcttcattga tcaggatttt tctggtgata atacccgttt aggtattggt ggcgaatact
     1801 ggcgagacta tttcaaaagt agcgttaacg gctatttccg catgagcggc tggcatgagt
     1861 catacaataa gaaagactat gatgagcgcc cagcaaatgg cttcgatatc cgttttaatg
     1921 gctatctacc gtcatatccg gcattaggcg ccaagctgat atatgagcag tattatggtg
     1981 ataatgttgc tttgtttaat tctgataagc tgcaatcgaa tcctggtgcg gcgaccgttg
     2041 gtgtaaacta tactccgatt cctctggtga cgatggggat cgattaccgt catggtacgg
     2101 gtaatgaaaa tgatctcctt tactcaatgc agttccgtta tcagtttgat aaatcgtggt
     2161 ctcagcaaat tgaaccacag tatgttaacg agttaagaac attatcaggc agccgttacg
     2221 atctggttca gcgtaataac aatattattc tggagtacaa gaagcaggat attctttctc
     2281 tgaatattcc gcatgatatt aatggtactg aacacagtac gcagaagatt cagttgatcg
     2341 ttaagagcaa atacggtctg gatcgtatcg tctgggatga tagtgcatta cgcagtcagg
     2401 gcggtcagat tcagcatagc ggaagccaaa gcgcacaaga ctaccaggct attttgcctg
     2461 cttatgtgca aggtggcagc aatatttata aagtgacggc tcgcgcctat gaccgtaatg
     2521 gcaatagctc taacaatgta cagcttacta ttaccgttct gtcgaatggt caagttgtcg
     2581 accaggttgg ggtaacggac tttacggcgg ataagacttc ggctaaagcg gataacgccg
     2641 ataccattac ttataccgcg acggtgaaaa agaatggggt agctcaggct aatgtccctg
     2701 tttcatttaa tattgtttca ggaactgcaa ctcttggggc aaatagtgcc aaaacggatg
     2761 ctaacggtaa ggcaaccgta acgttgaagt cgagtacgcc aggacaggtc gtcgtgtctg
     2821 ctaaaaccgc ggagatgact tcagcactta atgccagtgc ggttatattt tttgatggtg
     2881 cgactagtta ataaagcggc cgctgcagtc cggcaaaaaa gggcaaggtg tcaccaccct
     2941 gccctttttc tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg
     3001 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     3061 tctcgagtcc cgtcaagtca gcgtaatgct ctgccagtgt tacaaccaat taaccaattc
     3121 tgattagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc
     3181 aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt
     3241 ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca
     3301 acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac
     3361 gactgaatcc ggtgagaatg gcaaaagctt atgcatttct ttccagactt gttcaacagg
     3421 ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga
     3481 ttgcgcctga gcgagacgaa atacgcgatc gctgttaaaa ggacaattac aaacaggaat
     3541 cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg
     3601 atattcttct aatacctgga atgctgtttt cccggggatc gcagtggtga gtaaccatgc
     3661 atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca
     3721 gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag
     3781 aaacaactct ggcgcatcgg gcttcccata caatcgatag attgtcgcac ctgattgccc
     3841 gacattatcg cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg
     3901 cggcctcgag caagacgttt cccgttgaat atggctcata acaccccttg tattactgtt
     3961 tatgtaagca gacagtttta ttgttcatga tgatatattt ttatcttgtg caatgtaaca
     4021 tcagagattt tgagacacaa cgtggctttg ttgaataaat cgaacttttg ctgagttgaa
     4081 ggatcagatc acgcatcttc ccgacaacgc agaccgttcc gtggcaaagc aaaagttcaa
     4141 aatcaccaac tggtccacct acaacaaagc tctcatcaac cgtggctccc tcactttctg
     4201 gctggatgat ggggcgattc aggcctggta tgagtcagca acaccttctt cacgaggcag
     4261 acctcagcgc tagcggagtg tatactggct tactatgttg gcactgatga gggtgtcagt
     4321 gaagtgcttc atgtggcagg agaaaaaagg ctgcaccggt gcgtcagcag aatatgtgat
     4381 acaggatata ttccgcttcc tcgctcactg actcgctacg ctcggtcgtt cgactgcggc
     4441 gagcggaaat ggcttacgaa cggggcggag atttcctgga agatgccagg aagatactta
     4501 acagggaagt gagagggccg cggcaaagcc gtttttccat aggctccgcc cccctgacaa
     4561 gcatcacgaa atctgacgct caaatcagtg gtggcgaaac ccgacaggac tataaagata
     4621 ccaggcgttt cccctggcgg ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc
     4681 ggtgtcattc cgctgttatg gccgcgtttg tctcattcca cgcctgacac tcagttccgg
     4741 gtaggcagtt cgctccaagc tggactgtat gcacgaaccc cccgttcagt ccgaccgctg
     4801 cgccttatcc ggtaactatc gtcttgagtc caacccggaa agacatgcaa aagcaccact
     4861 ggcagcagcc actggtaatt gatttagagg agttagtctt gaagtcatgc gccggttaag
     4921 gctaaactga aaggacaagt tttggtgact gcgctcctcc aagccagtta cctcggttca
     4981 aagagttggt agctcagaga accttcgaaa aaccgccctg caaggcggtt ttttcgtttt
     5041 cagagcaaga gattacgcgc agaccaaaac gatctcaaga agatcatctt attaaggggt
     5101 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     5161 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     5221 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     5281 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     5341 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     5401 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     5461 caactttatc cgcctccatc cagtctattc catggtgcca cctgacgtct aagaaaccat
     5521 tattatcatg acattaacct ataaaaatag gcgtatcacg aggcagaatt tcagataaaa
     5581 aaaatcctta gctttcgcta aggatgattt ctggaattcg cggccgcttc tagag
//
LOCUS       pDSG290                 6701 bp    DNA     circular SYN 14-JUN-2018
DEFINITION  Synthetic plasmid.
ACCESSION   pDSG290
VERSION
KEYWORDS    .
SOURCE      Synthetic plasmid
  ORGANISM  Synthetic plasmid
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 6701)
  AUTHORS   Glass,D.S. and Riedel-Kruse,I.H.
  TITLE     A synthetic bacterial cell-cell adhesion toolbox for programming
            multicellular morphologies and patterns
  JOURNAL   Cell (2018) In press
REFERENCE   2  (bases 1 to 6701)
  AUTHORS   Glass,D.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2018) Bioengineering, Stanford University, 318
            Campus Drive, Clark Center E350, Stanford, CA 94305, USA
COMMENT     Bankit Comment: ALT EMAIL:dsglass@gmail.com
            Bankit Comment: Vecscreen Comment:Submitter says that this sequence
            represents a Cloning Vector
            Bankit Comment: TOTAL # OF SEQS:83.
FEATURES             Location/Qualifiers
     source          1..6701
                     /organism="Synthetic plasmid"
                     /mol_type="other DNA"
                     /db_xref="taxon:1440148"
     misc_feature    1..689
                     /label=BBa_I732084
     misc_feature    1..36
                     /label=pLacIQ (BBa_I4032, 1nt deletion)
     misc_feature    45..56
                     /label=BBa_J04500(1)
     misc_feature    45..56
                     /label=RBS (BBa_B0034)(1)
     CDS             63..686
                     /codon_start=1
                     /transl_table=11
                     /product="tetR ORF (BBa_I732080)"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     misc_feature    698..826
                     /label=Double terminator (BBa_B0015)
     misc_feature    835..896
                     /label=BBa_J13002
     misc_feature    835..888
                     /label=pTet (BBa_R0040)
     misc_feature    897..908
                     /label=BBa_J04500
     misc_feature    897..908
                     /label=RBS (BBa_B0034)
     CDS             915..2882
                     /codon_start=1
                     /transl_table=11
                     /product="Neae_removedRestrictionsites"
                     /translation="MITHGCYTRTRHKHKLKKTLIMLSAGLGLFFYVNQNSFANGENY
                     FKLGSDSKLLTHDSYQNRLFYTLKTGETVADLSKSQDINLSTIWSLNKHLYSSESEMM
                     KAAPGQQIILPLKKLPFEYSALPLLGSAPLVAAGGVAGHTNKLTKMSPDVTKSNMTDD
                     KALNYAAQQAASLGSQLQSRSLNGDYAKDTALGIAGNQASSQLQAWLQHYGTAEVNLQ
                     SGNNFDGSSLDFLLPFYDSEKMLAFGQVGARYIDSRFTANLGAGQRFFLPANMLGYNV
                     FIDQDFSGDNTRLGIGGEYWRDYFKSSVNGYFRMSGWHESYNKKDYDERPANGFDIRF
                     NGYLPSYPALGAKLIYEQYYGDNVALFNSDKLQSNPGAATVGVNYTPIPLVTMGIDYR
                     HGTGNENDLLYSMQFRYQFDKSWSQQIEPQYVNELRTLSGSRYDLVQRNNNIILEYKK
                     QDILSLNIPHDINGTEHSTQKIQLIVKSKYGLDRIVWDDSALRSQGGQIQHSGSQSAQ
                     DYQAILPAYVQGGSNIYKVTARAYDRNGNSSNNVQLTITVLSNGQVVDQVGVTDFTAD
                     KTSAKADNADTITYTATVKKNGVAQANVPVSFNIVSGTATLGANSAKTDANGKATVTL
                     KSSTPGQVVVSAKTAEMTSALNASAVIFFDGA"
     CDS             2889..3221
                     /codon_start=1
                     /transl_table=11
                     /product="Nanobody R4P43 against human P53 transactivation
                     do"
                     /translation="-
                     VQLQESGGGSVRPGESLRLSCIAAGTLFSGKAVGWYRQAPGKDRELVAALSSGRETYY
                     IGPVKGRFIISRDDNKDTLYLQMNNLKLEDTAIYTCRLGLHWGQGTQVTVSS"
     misc_feature    3222..3227
                     /label=Stop codons
     regulatory      3236..3364
                     /regulatory_class="terminator"
                     /label=terminator B0015
     misc_feature    3365..3385
                     /label=biobrick suffix
     misc_feature    3386..3457
                     /label=his operon terminator
     misc_feature    complement(3521..3538)
                     /label=VR primer site
     CDS             complement(3746..4696)
                     /codon_start=1
                     /transl_table=11
                     /product="repA"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRK
                     ERTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAK
                     FSSEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHK
                     ANIEISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTL
                     IFQVELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTS
                     FEAKFLSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     misc_feature    complement(4700..4712)
                     /label=RBS for repA
     regulatory      complement(4717..4736)
                     /regulatory_class="promoter"
                     /label=repA promoter
     CDS             complement(5582..6442)
                     /codon_start=1
                     /transl_table=11
                     /product="AmpR"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     misc_feature    complement(6443..6521)
                     /label=Promoter/RBS for AmpR
     misc_feature    6562..6582
                     /label=VF2 primer site
     misc_feature    6680..6701
                     /label=BioBricks prefix
BASE COUNT     1817 a   1432 c   1495 g   1957 t
ORIGIN      
        1 tggtgcaaaa cctttgcggt atggcatgat agcgcctact agagaaagag gagaaatact
       61 agatgtccag attagataaa agtaaagtga ttaacagcgc attagagctg cttaatgagg
      121 tcggaatcga aggtttaaca acccgtaaac tcgcccagaa gctaggtgta gagcagccta
      181 cattgtattg gcatgtaaaa aataagcggg ctttgctcga cgccttagcc attgagatgt
      241 tagataggca ccatactcac ttttgccctt tagaagggga aagctggcaa gattttttac
      301 gtaataacgc taaaagtttt agatgtgctt tactaagtca tcgcgatgga gcaaaagtac
      361 atttaggtac acggcctaca gaaaaacagt atgaaactct cgaaaatcaa ttagcctttt
      421 tatgccaaca aggtttttca ctagagaatg cattatatgc actcagcgct gtggggcatt
      481 ttactttagg ttgcgtattg gaagatcaag agcatcaagt cgctaaagaa gaaagggaaa
      541 cacctactac tgatagtatg ccgccattat tacgacaagc tatcgaatta tttgatcacc
      601 aaggtgcaga gccagccttc ttattcggcc ttgaattgat catatgcgga ttagaaaaac
      661 aacttaaatg tgaaagtggg tcctaataat actagagcca ggcatcaaat aaaacgaaag
      721 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
      781 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agagtcccta
      841 tcagtgatag agattgacat ccctatcagt gatagagata ctgagcacta ctagagaaag
      901 aggagaaata ctagatgatt actcatggtt gttatacccg gacccggcac aagcataagc
      961 taaaaaaaac attgattatg cttagtgctg gtttaggatt gtttttttat gttaatcaga
     1021 actcatttgc aaatggtgaa aattatttta aattgggttc ggattcaaaa ctgttaactc
     1081 atgatagcta tcagaatcgc cttttttata cgttgaaaac tggtgaaact gttgccgatc
     1141 tttctaaatc gcaagatatt aatttatcga cgatttggtc gttgaataag catttataca
     1201 gttctgaaag cgaaatgatg aaggccgcgc ctggtcagca gatcattttg ccactcaaaa
     1261 aacttccctt tgaatacagt gcactaccac ttttaggttc ggcacctctt gttgctgcgg
     1321 gtggtgttgc tggtcacacg aataaactga ctaaaatgtc cccggacgtg accaaaagca
     1381 acatgaccga tgacaaggca ttaaattatg cggcacaaca ggcggcgagt ctcggtagcc
     1441 agcttcagtc gcgatctctg aacggcgatt acgcgaaaga taccgctctt ggtatcgctg
     1501 gtaaccaggc ttcgtcacag ttgcaggcct ggttacaaca ttatggaacg gcagaggtta
     1561 atctgcaaag tggtaataac tttgacggta gttcactgga cttcttatta ccgttctatg
     1621 attccgaaaa aatgctggca tttggtcagg tcggagcgcg ttacattgac tcccgcttta
     1681 cggcaaattt aggtgcgggt cagcgttttt tccttcctgc aaacatgttg ggctataacg
     1741 tcttcattga tcaggatttt tctggtgata atacccgttt aggtattggt ggcgaatact
     1801 ggcgagacta tttcaaaagt agcgttaacg gctatttccg catgagcggc tggcatgagt
     1861 catacaataa gaaagactat gatgagcgcc cagcaaatgg cttcgatatc cgttttaatg
     1921 gctatctacc gtcatatccg gcattaggcg ccaagctgat atatgagcag tattatggtg
     1981 ataatgttgc tttgtttaat tctgataagc tgcaatcgaa tcctggtgcg gcgaccgttg
     2041 gtgtaaacta tactccgatt cctctggtga cgatggggat cgattaccgt catggtacgg
     2101 gtaatgaaaa tgatctcctt tactcaatgc agttccgtta tcagtttgat aaatcgtggt
     2161 ctcagcaaat tgaaccacag tatgttaacg agttaagaac attatcaggc agccgttacg
     2221 atctggttca gcgtaataac aatattattc tggagtacaa gaagcaggat attctttctc
     2281 tgaatattcc gcatgatatt aatggtactg aacacagtac gcagaagatt cagttgatcg
     2341 ttaagagcaa atacggtctg gatcgtatcg tctgggatga tagtgcatta cgcagtcagg
     2401 gcggtcagat tcagcatagc ggaagccaaa gcgcacaaga ctaccaggct attttgcctg
     2461 cttatgtgca aggtggcagc aatatttata aagtgacggc tcgcgcctat gaccgtaatg
     2521 gcaatagctc taacaatgta cagcttacta ttaccgttct gtcgaatggt caagttgtcg
     2581 accaggttgg ggtaacggac tttacggcgg ataagacttc ggctaaagcg gataacgccg
     2641 ataccattac ttataccgcg acggtgaaaa agaatggggt agctcaggct aatgtccctg
     2701 tttcatttaa tattgtttca ggaactgcaa ctcttggggc aaatagtgcc aaaacggatg
     2761 ctaacggtaa ggcaaccgta acgttgaagt cgagtacgcc aggacaggtc gtcgtgtctg
     2821 ctaaaaccgc ggagatgact tcagcactta atgccagtgc ggttatattt tttgatggtg
     2881 cgactagaca ggtgcagcta caggagtctg ggggaggctc ggtgcggcct ggggagtctc
     2941 ttcgactctc ctgtatagcc gctggaacct tgtttagtgg caaagccgtg ggctggtacc
     3001 gccaggctcc agggaaggac cgcgagctgg tcgcagctct tagtagtggt cgtgaaacat
     3061 actatatagg gcctgtgaag ggccgattca ttatctccag agacgataat aaagacacac
     3121 tgtatctgca aatgaacaac ctaaaacttg aggacacggc catctatacc tgtaggttag
     3181 gcctacattg gggccagggg acccaggtca ccgtctcctc ataataatac tagagccagg
     3241 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     3301 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
     3361 tatatactag tagcggccgc tgcagtccgg caaaaaaggg caaggtgtca ccaccctgcc
     3421 ctttttcttt aaaaccgaaa agattacttc gcgttatgca ggcttcctcg ctcactgact
     3481 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatct
     3541 cgaggttaca ttgtcgatct gttcatggtg aacagcttta aatgcaccaa aaactcgtaa
     3601 aagctctgat gtatctatct tttttacacc gttttcatct gtgcatatgg acagttttcc
     3661 ctttgatatc taacggtgaa cagttgttct acttttgttt gttagtcttg atgcttcact
     3721 gatagataca agagccataa gaacctcaga tccttccgta tttagccagt atgttctcta
     3781 gtgtggttcg ttgtttttgc gtgagccatg agaacgaacc attgagatca tgcttacttt
     3841 gcatgtcact caaaaatttt gcctcaaaac tggtgagctg aatttttgca gttaaagcat
     3901 cgtgtagtgt ttttcttagt ccgttacgta ggtaggaatc tgatgtaatg gttgttggta
     3961 ttttgtcacc attcattttt atctggttgt tctcaagttc ggttacgaga tccatttgtc
     4021 tatctagttc aacttggaaa atcaacgtat cagtcgggcg gcctcgctta tcaaccacca
     4081 atttcatatt gctgtaagtg tttaaatctt tacttattgg tttcaaaacc cattggttaa
     4141 gccttttaaa ctcatggtag ttattttcaa gcattaacat gaacttaaat tcatcaaggc
     4201 taatctctat atttgccttg tgagttttct tttgtgttag ttcttttaat aaccactcat
     4261 aaatcctcat agagtatttg ttttcaaaag acttaacatg ttccagatta tattttatga
     4321 atttttttaa ctggaaaaga taaggcaata tctcttcact aaaaactaat tctaattttt
     4381 cgcttgagaa cttggcatag tttgtccact ggaaaatctc aaagccttta accaaaggat
     4441 tcctgatttc cacagttctc gtcatcagct ctctggttgc tttagctaat acaccataag
     4501 cattttccct actgatgttc atcatctgag cgtattggtt ataagtgaac gataccgtcc
     4561 gttctttcct tgtagggttt tcaatcgtgg ggttgagtag tgccacacag cataaaatta
     4621 gcttggtttc atgctccgtt aagtcatagc gactaatcgc tagttcattt gctttgaaaa
     4681 caactaattc agacatacat ctcaattggt ctaggtgatt ttaatcacta taccaattga
     4741 gatgggctag tcaatgataa ttacatgtcc ttttcctttg agttgtgggt atctgtaaat
     4801 tctgctagac ctttgctgga aaacttgtaa attctgctag accctctgta aattccgcta
     4861 gacctttgtg tgtttttttt gtttatattc aagtggttat aatttataga ataaagaaag
     4921 aataaaaaaa gataaaaaga atagatccca gccctgtgta taactcacta ctttagtcag
     4981 ttccgcagta ttacaaaagg atgtcgcaaa cgctgtttgc tcctctacaa aacagacctt
     5041 aaaaccctaa aggcttaagt agcaccctcg caagctcggg caaatcgctg aatattcctt
     5101 ttgtctccga ccatcaggca cctgagtcgc tgtctttttc gtgacattca gttcgctgcg
     5161 ctcacggctc tggcagtgaa tgggggtaaa tggcactaca ggcgcctttt atggattcat
     5221 gcaaggaaac tacccataat acaagaaaag cccgtcacgg gcttctcagg gcgttttatg
     5281 gcgggtctgc tatgtggtgc tatctgactt tttgctgttc agcagttcct gccctctgat
     5341 tttccagtct gaccacttcg gattatcccg tgacaggtca ttcagactgg ctaatgcacc
     5401 cagtaaggca gcggtatcat caacaggctt acccgtctta ctgtccctag tgcttggatt
     5461 ctcaccaata aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca gatggagttc
     5521 tgaggtcatt actggatcta tcaacaggag tccaagcgag ctcgtaaact tggtctgaca
     5581 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     5641 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     5701 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     5761 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     5821 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     5881 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     5941 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     6001 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     6061 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     6121 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     6181 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     6241 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     6301 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     6361 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     6421 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     6481 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     6541 ttccgcgcac atttcccatg gtgccacctg acgtctaaga aaccattatt atcatgacat
     6601 taacctataa aaataggcgt atcacgaggc agaatttcag ataaaaaaaa tccttagctt
     6661 tcgctaagga tgatttctgg aattcgcggc cgcttctaga g
//
LOCUS       pDSG291                 6224 bp    DNA     circular SYN 14-JUN-2018
DEFINITION  Synthetic plasmid.
ACCESSION   pDSG291
VERSION
KEYWORDS    .
SOURCE      Synthetic plasmid
  ORGANISM  Synthetic plasmid
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 6224)
  AUTHORS   Glass,D.S. and Riedel-Kruse,I.H.
  TITLE     A synthetic bacterial cell-cell adhesion toolbox for programming
            multicellular morphologies and patterns
  JOURNAL   Cell (2018) In press
REFERENCE   2  (bases 1 to 6224)
  AUTHORS   Glass,D.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2018) Bioengineering, Stanford University, 318
            Campus Drive, Clark Center E350, Stanford, CA 94305, USA
COMMENT     Bankit Comment: ALT EMAIL:dsglass@gmail.com
            Bankit Comment: Vecscreen Comment:Submitter says that this sequence
            represents a Cloning Vector
            Bankit Comment: TOTAL # OF SEQS:83.
FEATURES             Location/Qualifiers
     source          1..6224
                     /organism="Synthetic plasmid"
                     /mol_type="other DNA"
                     /db_xref="taxon:1440148"
     misc_feature    1..689
                     /label=BBa_I732084
     misc_feature    1..36
                     /label=pLacIQ (BBa_I4032, 1nt deletion)
     misc_feature    45..56
                     /label=BBa_J04500(1)
     misc_feature    45..56
                     /label=RBS (BBa_B0034)(1)
     CDS             63..686
                     /codon_start=1
                     /transl_table=11
                     /product="tetR ORF (BBa_I732080)"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     misc_feature    698..826
                     /label=Double terminator (BBa_B0015)
     misc_feature    835..896
                     /label=BBa_J13002
     misc_feature    835..888
                     /label=pTet (BBa_R0040)
     misc_feature    897..908
                     /label=BBa_J04500
     misc_feature    897..908
                     /label=RBS (BBa_B0034)
     CDS             915..2882
                     /codon_start=1
                     /transl_table=11
                     /product="Neae_removedRestrictionsites"
                     /translation="MITHGCYTRTRHKHKLKKTLIMLSAGLGLFFYVNQNSFANGENY
                     FKLGSDSKLLTHDSYQNRLFYTLKTGETVADLSKSQDINLSTIWSLNKHLYSSESEMM
                     KAAPGQQIILPLKKLPFEYSALPLLGSAPLVAAGGVAGHTNKLTKMSPDVTKSNMTDD
                     KALNYAAQQAASLGSQLQSRSLNGDYAKDTALGIAGNQASSQLQAWLQHYGTAEVNLQ
                     SGNNFDGSSLDFLLPFYDSEKMLAFGQVGARYIDSRFTANLGAGQRFFLPANMLGYNV
                     FIDQDFSGDNTRLGIGGEYWRDYFKSSVNGYFRMSGWHESYNKKDYDERPANGFDIRF
                     NGYLPSYPALGAKLIYEQYYGDNVALFNSDKLQSNPGAATVGVNYTPIPLVTMGIDYR
                     HGTGNENDLLYSMQFRYQFDKSWSQQIEPQYVNELRTLSGSRYDLVQRNNNIILEYKK
                     QDILSLNIPHDINGTEHSTQKIQLIVKSKYGLDRIVWDDSALRSQGGQIQHSGSQSAQ
                     DYQAILPAYVQGGSNIYKVTARAYDRNGNSSNNVQLTITVLSNGQVVDQVGVTDFTAD
                     KTSAKADNADTITYTATVKKNGVAQANVPVSFNIVSGTATLGANSAKTDANGKATVTL
                     KSSTPGQVVVSAKTAEMTSALNASAVIFFDGA"
     misc_feature    2883..2908
                     /label=RFC[23] biobrick suffix (partial)
     misc_feature    2909..2980
                     /label=his operon terminator
     misc_feature    complement(3044..3061)
                     /label=VR primer site
     CDS             complement(3269..4219)
                     /codon_start=1
                     /transl_table=11
                     /product="repA"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRK
                     ERTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAK
                     FSSEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHK
                     ANIEISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTL
                     IFQVELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTS
                     FEAKFLSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     misc_feature    complement(4223..4235)
                     /label=RBS for repA
     regulatory      complement(4240..4259)
                     /regulatory_class="promoter"
                     /label=repA promoter
     CDS             complement(5105..5965)
                     /codon_start=1
                     /transl_table=11
                     /product="AmpR"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     misc_feature    complement(5966..6044)
                     /label=Promoter/RBS for AmpR
     misc_feature    6085..6105
                     /label=VF2 primer site
     misc_feature    6203..6224
                     /label=BioBricks prefix
BASE COUNT     1705 a   1315 c   1362 g   1842 t
ORIGIN      
        1 tggtgcaaaa cctttgcggt atggcatgat agcgcctact agagaaagag gagaaatact
       61 agatgtccag attagataaa agtaaagtga ttaacagcgc attagagctg cttaatgagg
      121 tcggaatcga aggtttaaca acccgtaaac tcgcccagaa gctaggtgta gagcagccta
      181 cattgtattg gcatgtaaaa aataagcggg ctttgctcga cgccttagcc attgagatgt
      241 tagataggca ccatactcac ttttgccctt tagaagggga aagctggcaa gattttttac
      301 gtaataacgc taaaagtttt agatgtgctt tactaagtca tcgcgatgga gcaaaagtac
      361 atttaggtac acggcctaca gaaaaacagt atgaaactct cgaaaatcaa ttagcctttt
      421 tatgccaaca aggtttttca ctagagaatg cattatatgc actcagcgct gtggggcatt
      481 ttactttagg ttgcgtattg gaagatcaag agcatcaagt cgctaaagaa gaaagggaaa
      541 cacctactac tgatagtatg ccgccattat tacgacaagc tatcgaatta tttgatcacc
      601 aaggtgcaga gccagccttc ttattcggcc ttgaattgat catatgcgga ttagaaaaac
      661 aacttaaatg tgaaagtggg tcctaataat actagagcca ggcatcaaat aaaacgaaag
      721 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
      781 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agagtcccta
      841 tcagtgatag agattgacat ccctatcagt gatagagata ctgagcacta ctagagaaag
      901 aggagaaata ctagatgatt actcatggtt gttatacccg gacccggcac aagcataagc
      961 taaaaaaaac attgattatg cttagtgctg gtttaggatt gtttttttat gttaatcaga
     1021 actcatttgc aaatggtgaa aattatttta aattgggttc ggattcaaaa ctgttaactc
     1081 atgatagcta tcagaatcgc cttttttata cgttgaaaac tggtgaaact gttgccgatc
     1141 tttctaaatc gcaagatatt aatttatcga cgatttggtc gttgaataag catttataca
     1201 gttctgaaag cgaaatgatg aaggccgcgc ctggtcagca gatcattttg ccactcaaaa
     1261 aacttccctt tgaatacagt gcactaccac ttttaggttc ggcacctctt gttgctgcgg
     1321 gtggtgttgc tggtcacacg aataaactga ctaaaatgtc cccggacgtg accaaaagca
     1381 acatgaccga tgacaaggca ttaaattatg cggcacaaca ggcggcgagt ctcggtagcc
     1441 agcttcagtc gcgatctctg aacggcgatt acgcgaaaga taccgctctt ggtatcgctg
     1501 gtaaccaggc ttcgtcacag ttgcaggcct ggttacaaca ttatggaacg gcagaggtta
     1561 atctgcaaag tggtaataac tttgacggta gttcactgga cttcttatta ccgttctatg
     1621 attccgaaaa aatgctggca tttggtcagg tcggagcgcg ttacattgac tcccgcttta
     1681 cggcaaattt aggtgcgggt cagcgttttt tccttcctgc aaacatgttg ggctataacg
     1741 tcttcattga tcaggatttt tctggtgata atacccgttt aggtattggt ggcgaatact
     1801 ggcgagacta tttcaaaagt agcgttaacg gctatttccg catgagcggc tggcatgagt
     1861 catacaataa gaaagactat gatgagcgcc cagcaaatgg cttcgatatc cgttttaatg
     1921 gctatctacc gtcatatccg gcattaggcg ccaagctgat atatgagcag tattatggtg
     1981 ataatgttgc tttgtttaat tctgataagc tgcaatcgaa tcctggtgcg gcgaccgttg
     2041 gtgtaaacta tactccgatt cctctggtga cgatggggat cgattaccgt catggtacgg
     2101 gtaatgaaaa tgatctcctt tactcaatgc agttccgtta tcagtttgat aaatcgtggt
     2161 ctcagcaaat tgaaccacag tatgttaacg agttaagaac attatcaggc agccgttacg
     2221 atctggttca gcgtaataac aatattattc tggagtacaa gaagcaggat attctttctc
     2281 tgaatattcc gcatgatatt aatggtactg aacacagtac gcagaagatt cagttgatcg
     2341 ttaagagcaa atacggtctg gatcgtatcg tctgggatga tagtgcatta cgcagtcagg
     2401 gcggtcagat tcagcatagc ggaagccaaa gcgcacaaga ctaccaggct attttgcctg
     2461 cttatgtgca aggtggcagc aatatttata aagtgacggc tcgcgcctat gaccgtaatg
     2521 gcaatagctc taacaatgta cagcttacta ttaccgttct gtcgaatggt caagttgtcg
     2581 accaggttgg ggtaacggac tttacggcgg ataagacttc ggctaaagcg gataacgccg
     2641 ataccattac ttataccgcg acggtgaaaa agaatggggt agctcaggct aatgtccctg
     2701 tttcatttaa tattgtttca ggaactgcaa ctcttggggc aaatagtgcc aaaacggatg
     2761 ctaacggtaa ggcaaccgta acgttgaagt cgagtacgcc aggacaggtc gtcgtgtctg
     2821 ctaaaaccgc ggagatgact tcagcactta atgccagtgc ggttatattt tttgatggtg
     2881 cgactagtta ataaagcggc cgctgcagtc cggcaaaaaa gggcaaggtg tcaccaccct
     2941 gccctttttc tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg
     3001 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     3061 tctcgaggtt acattgtcga tctgttcatg gtgaacagct ttaaatgcac caaaaactcg
     3121 taaaagctct gatgtatcta tcttttttac accgttttca tctgtgcata tggacagttt
     3181 tccctttgat atctaacggt gaacagttgt tctacttttg tttgttagtc ttgatgcttc
     3241 actgatagat acaagagcca taagaacctc agatccttcc gtatttagcc agtatgttct
     3301 ctagtgtggt tcgttgtttt tgcgtgagcc atgagaacga accattgaga tcatgcttac
     3361 tttgcatgtc actcaaaaat tttgcctcaa aactggtgag ctgaattttt gcagttaaag
     3421 catcgtgtag tgtttttctt agtccgttac gtaggtagga atctgatgta atggttgttg
     3481 gtattttgtc accattcatt tttatctggt tgttctcaag ttcggttacg agatccattt
     3541 gtctatctag ttcaacttgg aaaatcaacg tatcagtcgg gcggcctcgc ttatcaacca
     3601 ccaatttcat attgctgtaa gtgtttaaat ctttacttat tggtttcaaa acccattggt
     3661 taagcctttt aaactcatgg tagttatttt caagcattaa catgaactta aattcatcaa
     3721 ggctaatctc tatatttgcc ttgtgagttt tcttttgtgt tagttctttt aataaccact
     3781 cataaatcct catagagtat ttgttttcaa aagacttaac atgttccaga ttatatttta
     3841 tgaatttttt taactggaaa agataaggca atatctcttc actaaaaact aattctaatt
     3901 tttcgcttga gaacttggca tagtttgtcc actggaaaat ctcaaagcct ttaaccaaag
     3961 gattcctgat ttccacagtt ctcgtcatca gctctctggt tgctttagct aatacaccat
     4021 aagcattttc cctactgatg ttcatcatct gagcgtattg gttataagtg aacgataccg
     4081 tccgttcttt ccttgtaggg ttttcaatcg tggggttgag tagtgccaca cagcataaaa
     4141 ttagcttggt ttcatgctcc gttaagtcat agcgactaat cgctagttca tttgctttga
     4201 aaacaactaa ttcagacata catctcaatt ggtctaggtg attttaatca ctataccaat
     4261 tgagatgggc tagtcaatga taattacatg tccttttcct ttgagttgtg ggtatctgta
     4321 aattctgcta gacctttgct ggaaaacttg taaattctgc tagaccctct gtaaattccg
     4381 ctagaccttt gtgtgttttt tttgtttata ttcaagtggt tataatttat agaataaaga
     4441 aagaataaaa aaagataaaa agaatagatc ccagccctgt gtataactca ctactttagt
     4501 cagttccgca gtattacaaa aggatgtcgc aaacgctgtt tgctcctcta caaaacagac
     4561 cttaaaaccc taaaggctta agtagcaccc tcgcaagctc gggcaaatcg ctgaatattc
     4621 cttttgtctc cgaccatcag gcacctgagt cgctgtcttt ttcgtgacat tcagttcgct
     4681 gcgctcacgg ctctggcagt gaatgggggt aaatggcact acaggcgcct tttatggatt
     4741 catgcaagga aactacccat aatacaagaa aagcccgtca cgggcttctc agggcgtttt
     4801 atggcgggtc tgctatgtgg tgctatctga ctttttgctg ttcagcagtt cctgccctct
     4861 gattttccag tctgaccact tcggattatc ccgtgacagg tcattcagac tggctaatgc
     4921 acccagtaag gcagcggtat catcaacagg cttacccgtc ttactgtccc tagtgcttgg
     4981 attctcacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag
     5041 ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgtaa acttggtctg
     5101 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     5161 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     5221 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     5281 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     5341 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     5401 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     5461 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     5521 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     5581 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     5641 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     5701 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     5761 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     5821 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     5881 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     5941 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     6001 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     6061 gggttccgcg cacatttccc atggtgccac ctgacgtcta agaaaccatt attatcatga
     6121 cattaaccta taaaaatagg cgtatcacga ggcagaattt cagataaaaa aaatccttag
     6181 ctttcgctaa ggatgatttc tggaattcgc ggccgcttct agag
//
LOCUS       pDSG289                 6748 bp    DNA     circular SYN 14-JUN-2018
DEFINITION  Synthetic plasmid.
ACCESSION   pDSG289
VERSION
KEYWORDS    .
SOURCE      Synthetic plasmid
  ORGANISM  Synthetic plasmid
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 6748)
  AUTHORS   Glass,D.S. and Riedel-Kruse,I.H.
  TITLE     A synthetic bacterial cell-cell adhesion toolbox for programming
            multicellular morphologies and patterns
  JOURNAL   Cell (2018) In press
REFERENCE   2  (bases 1 to 6748)
  AUTHORS   Glass,D.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2018) Bioengineering, Stanford University, 318
            Campus Drive, Clark Center E350, Stanford, CA 94305, USA
COMMENT     Bankit Comment: ALT EMAIL:dsglass@gmail.com
            Bankit Comment: Vecscreen Comment:Submitter says that this sequence
            represents a Cloning Vector
            Bankit Comment: TOTAL # OF SEQS:83.
FEATURES             Location/Qualifiers
     source          1..6748
                     /organism="Synthetic plasmid"
                     /mol_type="other DNA"
                     /db_xref="taxon:1440148"
     misc_feature    1..689
                     /label=BBa_I732084
     misc_feature    1..36
                     /label=pLacIQ (BBa_I4032, 1nt deletion)
     misc_feature    45..56
                     /label=BBa_J04500(1)
     misc_feature    45..56
                     /label=RBS (BBa_B0034)(1)
     CDS             63..686
                     /codon_start=1
                     /transl_table=11
                     /product="tetR ORF (BBa_I732080)"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     misc_feature    698..826
                     /label=Double terminator (BBa_B0015)
     misc_feature    835..896
                     /label=BBa_J13002
     misc_feature    835..888
                     /label=pTet (BBa_R0040)
     misc_feature    897..908
                     /label=BBa_J04500
     misc_feature    897..908
                     /label=RBS (BBa_B0034)
     CDS             915..2882
                     /codon_start=1
                     /transl_table=11
                     /product="Neae_removedRestrictionsites"
                     /translation="MITHGCYTRTRHKHKLKKTLIMLSAGLGLFFYVNQNSFANGENY
                     FKLGSDSKLLTHDSYQNRLFYTLKTGETVADLSKSQDINLSTIWSLNKHLYSSESEMM
                     KAAPGQQIILPLKKLPFEYSALPLLGSAPLVAAGGVAGHTNKLTKMSPDVTKSNMTDD
                     KALNYAAQQAASLGSQLQSRSLNGDYAKDTALGIAGNQASSQLQAWLQHYGTAEVNLQ
                     SGNNFDGSSLDFLLPFYDSEKMLAFGQVGARYIDSRFTANLGAGQRFFLPANMLGYNV
                     FIDQDFSGDNTRLGIGGEYWRDYFKSSVNGYFRMSGWHESYNKKDYDERPANGFDIRF
                     NGYLPSYPALGAKLIYEQYYGDNVALFNSDKLQSNPGAATVGVNYTPIPLVTMGIDYR
                     HGTGNENDLLYSMQFRYQFDKSWSQQIEPQYVNELRTLSGSRYDLVQRNNNIILEYKK
                     QDILSLNIPHDINGTEHSTQKIQLIVKSKYGLDRIVWDDSALRSQGGQIQHSGSQSAQ
                     DYQAILPAYVQGGSNIYKVTARAYDRNGNSSNNVQLTITVLSNGQVVDQVGVTDFTAD
                     KTSAKADNADTITYTATVKKNGVAQANVPVSFNIVSGTATLGANSAKTDANGKATVTL
                     KSSTPGQVVVSAKTAEMTSALNASAVIFFDGA"
     CDS             2889..3266
                     /codon_start=1
                     /transl_table=11
                     /product="Nanobody against EPEA tag"
                     /translation="-
                     GQLVESGGGSVQAGGSLRLSCAASGIDSSSYCMGWFRQRPGKEREGVARINGLGGVKT
                     AYADSVKDRFTISRDNAENTVYLQMNSLKPEDTAIYYCAAKFSPGYCGGSWSNFGYWG
                     QGTQVTVSS"
     misc_feature    3267..3272
                     /label=Stop codons
     regulatory      3281..3409
                     /regulatory_class="terminator"
                     /label=terminator B0015
     misc_feature    3410..3430
                     /label=biobrick suffix
     misc_feature    3431..3504
                     /label=his operon terminator
     misc_feature    complement(3568..3585)
                     /label=VR primer site
     CDS             complement(3793..4743)
                     /codon_start=1
                     /transl_table=11
                     /product="repA"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRK
                     ERTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAK
                     FSSEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHK
                     ANIEISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTL
                     IFQVELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTS
                     FEAKFLSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     misc_feature    complement(4747..4759)
                     /label=RBS for repA
     regulatory      complement(4764..4783)
                     /regulatory_class="promoter"
                     /label=repA promoter
     CDS             complement(5629..6489)
                     /codon_start=1
                     /transl_table=11
                     /product="AmpR"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     misc_feature    complement(6490..6568)
                     /label=Promoter/RBS for AmpR
     misc_feature    6609..6629
                     /label=VF2 primer site
     misc_feature    6727..6748
                     /label=BioBricks prefix
BASE COUNT     1828 a   1424 c   1511 g   1985 t
ORIGIN      
        1 tggtgcaaaa cctttgcggt atggcatgat agcgcctact agagaaagag gagaaatact
       61 agatgtccag attagataaa agtaaagtga ttaacagcgc attagagctg cttaatgagg
      121 tcggaatcga aggtttaaca acccgtaaac tcgcccagaa gctaggtgta gagcagccta
      181 cattgtattg gcatgtaaaa aataagcggg ctttgctcga cgccttagcc attgagatgt
      241 tagataggca ccatactcac ttttgccctt tagaagggga aagctggcaa gattttttac
      301 gtaataacgc taaaagtttt agatgtgctt tactaagtca tcgcgatgga gcaaaagtac
      361 atttaggtac acggcctaca gaaaaacagt atgaaactct cgaaaatcaa ttagcctttt
      421 tatgccaaca aggtttttca ctagagaatg cattatatgc actcagcgct gtggggcatt
      481 ttactttagg ttgcgtattg gaagatcaag agcatcaagt cgctaaagaa gaaagggaaa
      541 cacctactac tgatagtatg ccgccattat tacgacaagc tatcgaatta tttgatcacc
      601 aaggtgcaga gccagccttc ttattcggcc ttgaattgat catatgcgga ttagaaaaac
      661 aacttaaatg tgaaagtggg tcctaataat actagagcca ggcatcaaat aaaacgaaag
      721 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
      781 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agagtcccta
      841 tcagtgatag agattgacat ccctatcagt gatagagata ctgagcacta ctagagaaag
      901 aggagaaata ctagatgatt actcatggtt gttatacccg gacccggcac aagcataagc
      961 taaaaaaaac attgattatg cttagtgctg gtttaggatt gtttttttat gttaatcaga
     1021 actcatttgc aaatggtgaa aattatttta aattgggttc ggattcaaaa ctgttaactc
     1081 atgatagcta tcagaatcgc cttttttata cgttgaaaac tggtgaaact gttgccgatc
     1141 tttctaaatc gcaagatatt aatttatcga cgatttggtc gttgaataag catttataca
     1201 gttctgaaag cgaaatgatg aaggccgcgc ctggtcagca gatcattttg ccactcaaaa
     1261 aacttccctt tgaatacagt gcactaccac ttttaggttc ggcacctctt gttgctgcgg
     1321 gtggtgttgc tggtcacacg aataaactga ctaaaatgtc cccggacgtg accaaaagca
     1381 acatgaccga tgacaaggca ttaaattatg cggcacaaca ggcggcgagt ctcggtagcc
     1441 agcttcagtc gcgatctctg aacggcgatt acgcgaaaga taccgctctt ggtatcgctg
     1501 gtaaccaggc ttcgtcacag ttgcaggcct ggttacaaca ttatggaacg gcagaggtta
     1561 atctgcaaag tggtaataac tttgacggta gttcactgga cttcttatta ccgttctatg
     1621 attccgaaaa aatgctggca tttggtcagg tcggagcgcg ttacattgac tcccgcttta
     1681 cggcaaattt aggtgcgggt cagcgttttt tccttcctgc aaacatgttg ggctataacg
     1741 tcttcattga tcaggatttt tctggtgata atacccgttt aggtattggt ggcgaatact
     1801 ggcgagacta tttcaaaagt agcgttaacg gctatttccg catgagcggc tggcatgagt
     1861 catacaataa gaaagactat gatgagcgcc cagcaaatgg cttcgatatc cgttttaatg
     1921 gctatctacc gtcatatccg gcattaggcg ccaagctgat atatgagcag tattatggtg
     1981 ataatgttgc tttgtttaat tctgataagc tgcaatcgaa tcctggtgcg gcgaccgttg
     2041 gtgtaaacta tactccgatt cctctggtga cgatggggat cgattaccgt catggtacgg
     2101 gtaatgaaaa tgatctcctt tactcaatgc agttccgtta tcagtttgat aaatcgtggt
     2161 ctcagcaaat tgaaccacag tatgttaacg agttaagaac attatcaggc agccgttacg
     2221 atctggttca gcgtaataac aatattattc tggagtacaa gaagcaggat attctttctc
     2281 tgaatattcc gcatgatatt aatggtactg aacacagtac gcagaagatt cagttgatcg
     2341 ttaagagcaa atacggtctg gatcgtatcg tctgggatga tagtgcatta cgcagtcagg
     2401 gcggtcagat tcagcatagc ggaagccaaa gcgcacaaga ctaccaggct attttgcctg
     2461 cttatgtgca aggtggcagc aatatttata aagtgacggc tcgcgcctat gaccgtaatg
     2521 gcaatagctc taacaatgta cagcttacta ttaccgttct gtcgaatggt caagttgtcg
     2581 accaggttgg ggtaacggac tttacggcgg ataagacttc ggctaaagcg gataacgccg
     2641 ataccattac ttataccgcg acggtgaaaa agaatggggt agctcaggct aatgtccctg
     2701 tttcatttaa tattgtttca ggaactgcaa ctcttggggc aaatagtgcc aaaacggatg
     2761 ctaacggtaa ggcaaccgta acgttgaagt cgagtacgcc aggacaggtc gtcgtgtctg
     2821 ctaaaaccgc ggagatgact tcagcactta atgccagtgc ggttatattt tttgatggtg
     2881 cgactagaca agggcagctt gttgagagcg gggggggaag cgtacaagcc gggggatcgc
     2941 tgcgcttgtc gtgtgcagct tccggtattg attccagctc atactgtatg ggttggtttc
     3001 gtcagcgtcc gggaaaggag cgcgagggtg tcgcacgtat caacgggttg ggcggcgtta
     3061 aaacagctta cgctgactct gttaaagatc gtttcaccat tagtcgtgat aatgcagaaa
     3121 atactgttta tttgcaaatg aactccttga aacccgaaga tacagctatt tattattgtg
     3181 ctgccaagtt tagtccaggt tattgcggag gatcgtggag caatttcgga tactggggtc
     3241 aaggaacgca agtgaccgta tcttcgtaat aatactagag ccaggcatca aataaaacga
     3301 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     3361 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat actagtagcg
     3421 gccgctgcag tccggcaaaa aaacgggcaa ggtgtcacca ccctgccctt tttctttaaa
     3481 accgaaaaga ttacttcgcg ttatgcaggc ttcctcgctc actgactcgc tgcgctcggt
     3541 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatctcga ggttacattg
     3601 tcgatctgtt catggtgaac agctttaaat gcaccaaaaa ctcgtaaaag ctctgatgta
     3661 tctatctttt ttacaccgtt ttcatctgtg catatggaca gttttccctt tgatatctaa
     3721 cggtgaacag ttgttctact tttgtttgtt agtcttgatg cttcactgat agatacaaga
     3781 gccataagaa cctcagatcc ttccgtattt agccagtatg ttctctagtg tggttcgttg
     3841 tttttgcgtg agccatgaga acgaaccatt gagatcatgc ttactttgca tgtcactcaa
     3901 aaattttgcc tcaaaactgg tgagctgaat ttttgcagtt aaagcatcgt gtagtgtttt
     3961 tcttagtccg ttacgtaggt aggaatctga tgtaatggtt gttggtattt tgtcaccatt
     4021 catttttatc tggttgttct caagttcggt tacgagatcc atttgtctat ctagttcaac
     4081 ttggaaaatc aacgtatcag tcgggcggcc tcgcttatca accaccaatt tcatattgct
     4141 gtaagtgttt aaatctttac ttattggttt caaaacccat tggttaagcc ttttaaactc
     4201 atggtagtta ttttcaagca ttaacatgaa cttaaattca tcaaggctaa tctctatatt
     4261 tgccttgtga gttttctttt gtgttagttc ttttaataac cactcataaa tcctcataga
     4321 gtatttgttt tcaaaagact taacatgttc cagattatat tttatgaatt tttttaactg
     4381 gaaaagataa ggcaatatct cttcactaaa aactaattct aatttttcgc ttgagaactt
     4441 ggcatagttt gtccactgga aaatctcaaa gcctttaacc aaaggattcc tgatttccac
     4501 agttctcgtc atcagctctc tggttgcttt agctaataca ccataagcat tttccctact
     4561 gatgttcatc atctgagcgt attggttata agtgaacgat accgtccgtt ctttccttgt
     4621 agggttttca atcgtggggt tgagtagtgc cacacagcat aaaattagct tggtttcatg
     4681 ctccgttaag tcatagcgac taatcgctag ttcatttgct ttgaaaacaa ctaattcaga
     4741 catacatctc aattggtcta ggtgatttta atcactatac caattgagat gggctagtca
     4801 atgataatta catgtccttt tcctttgagt tgtgggtatc tgtaaattct gctagacctt
     4861 tgctggaaaa cttgtaaatt ctgctagacc ctctgtaaat tccgctagac ctttgtgtgt
     4921 tttttttgtt tatattcaag tggttataat ttatagaata aagaaagaat aaaaaaagat
     4981 aaaaagaata gatcccagcc ctgtgtataa ctcactactt tagtcagttc cgcagtatta
     5041 caaaaggatg tcgcaaacgc tgtttgctcc tctacaaaac agaccttaaa accctaaagg
     5101 cttaagtagc accctcgcaa gctcgggcaa atcgctgaat attccttttg tctccgacca
     5161 tcaggcacct gagtcgctgt ctttttcgtg acattcagtt cgctgcgctc acggctctgg
     5221 cagtgaatgg gggtaaatgg cactacaggc gccttttatg gattcatgca aggaaactac
     5281 ccataataca agaaaagccc gtcacgggct tctcagggcg ttttatggcg ggtctgctat
     5341 gtggtgctat ctgacttttt gctgttcagc agttcctgcc ctctgatttt ccagtctgac
     5401 cacttcggat tatcccgtga caggtcattc agactggcta atgcacccag taaggcagcg
     5461 gtatcatcaa caggcttacc cgtcttactg tccctagtgc ttggattctc accaataaaa
     5521 aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact
     5581 ggatctatca acaggagtcc aagcgagctc gtaaacttgg tctgacagtt accaatgctt
     5641 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     5701 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     5761 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     5821 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     5881 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     5941 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     6001 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     6061 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     6121 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     6181 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     6241 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     6301 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     6361 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     6421 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     6481 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     6541 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     6601 tcccatggtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     6661 taggcgtatc acgaggcaga atttcagata aaaaaaatcc ttagctttcg ctaaggatga
     6721 tttctggaat tcgcggccgc ttctagag
//
LOCUS       pDSG288                 6676 bp    DNA     circular SYN 14-JUN-2018
DEFINITION  Synthetic plasmid.
ACCESSION   pDSG288
VERSION
KEYWORDS    .
SOURCE      Synthetic plasmid
  ORGANISM  Synthetic plasmid
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 6676)
  AUTHORS   Glass,D.S. and Riedel-Kruse,I.H.
  TITLE     A synthetic bacterial cell-cell adhesion toolbox for programming
            multicellular morphologies and patterns
  JOURNAL   Cell (2018) In press
REFERENCE   2  (bases 1 to 6676)
  AUTHORS   Glass,D.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2018) Bioengineering, Stanford University, 318
            Campus Drive, Clark Center E350, Stanford, CA 94305, USA
COMMENT     Bankit Comment: ALT EMAIL:dsglass@gmail.com
            Bankit Comment: Vecscreen Comment:Submitter says that this sequence
            represents a Cloning Vector
            Bankit Comment: TOTAL # OF SEQS:83.
FEATURES             Location/Qualifiers
     source          1..6676
                     /organism="Synthetic plasmid"
                     /mol_type="other DNA"
                     /db_xref="taxon:1440148"
     misc_feature    1..689
                     /label=BBa_I732084
     misc_feature    1..36
                     /label=pLacIQ (BBa_I4032, 1nt deletion)
     misc_feature    45..56
                     /label=BBa_J04500(1)
     misc_feature    45..56
                     /label=RBS (BBa_B0034)(1)
     CDS             63..686
                     /codon_start=1
                     /transl_table=11
                     /product="tetR ORF (BBa_I732080)"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     misc_feature    698..826
                     /label=Double terminator (BBa_B0015)
     misc_feature    835..896
                     /label=BBa_J13002
     misc_feature    835..888
                     /label=pTet (BBa_R0040)
     misc_feature    897..908
                     /label=BBa_J04500
     misc_feature    897..908
                     /label=RBS (BBa_B0034)
     CDS             915..2882
                     /codon_start=1
                     /transl_table=11
                     /product="Neae_removedRestrictionsites"
                     /translation="MITHGCYTRTRHKHKLKKTLIMLSAGLGLFFYVNQNSFANGENY
                     FKLGSDSKLLTHDSYQNRLFYTLKTGETVADLSKSQDINLSTIWSLNKHLYSSESEMM
                     KAAPGQQIILPLKKLPFEYSALPLLGSAPLVAAGGVAGHTNKLTKMSPDVTKSNMTDD
                     KALNYAAQQAASLGSQLQSRSLNGDYAKDTALGIAGNQASSQLQAWLQHYGTAEVNLQ
                     SGNNFDGSSLDFLLPFYDSEKMLAFGQVGARYIDSRFTANLGAGQRFFLPANMLGYNV
                     FIDQDFSGDNTRLGIGGEYWRDYFKSSVNGYFRMSGWHESYNKKDYDERPANGFDIRF
                     NGYLPSYPALGAKLIYEQYYGDNVALFNSDKLQSNPGAATVGVNYTPIPLVTMGIDYR
                     HGTGNENDLLYSMQFRYQFDKSWSQQIEPQYVNELRTLSGSRYDLVQRNNNIILEYKK
                     QDILSLNIPHDINGTEHSTQKIQLIVKSKYGLDRIVWDDSALRSQGGQIQHSGSQSAQ
                     DYQAILPAYVQGGSNIYKVTARAYDRNGNSSNNVQLTITVLSNGQVVDQVGVTDFTAD
                     KTSAKADNADTITYTATVKKNGVAQANVPVSFNIVSGTATLGANSAKTDANGKATVTL
                     KSSTPGQVVVSAKTAEMTSALNASAVIFFDGA"
     CDS             2889..3194
                     /codon_start=1
                     /transl_table=11
                     /product="human P53 transactivation domain"
                     /translation="MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLM
                     LSPDDIEQWFTEDPGPDEAPRMPEAAPRVAPAPAAPTPAAPAPAPSWPLSSSVPSQKT
                     "
     misc_feature    3195..3200
                     /label=Stop codons
     regulatory      3209..3337
                     /regulatory_class="terminator"
                     /label=terminator B0015
     misc_feature    3338..3358
                     /label=biobrick suffix
     misc_feature    3359..3432
                     /label=his operon terminator
     misc_feature    complement(3496..3513)
                     /label=VR primer site
     CDS             complement(3721..4671)
                     /codon_start=1
                     /transl_table=11
                     /product="repA"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRK
                     ERTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAK
                     FSSEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHK
                     ANIEISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTL
                     IFQVELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTS
                     FEAKFLSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     misc_feature    complement(4675..4687)
                     /label=RBS for repA
     regulatory      complement(4692..4711)
                     /regulatory_class="promoter"
                     /label=repA promoter
     CDS             complement(5557..6417)
                     /codon_start=1
                     /transl_table=11
                     /product="AmpR"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     misc_feature    complement(6418..6496)
                     /label=Promoter/RBS for AmpR
     misc_feature    6537..6557
                     /label=VF2 primer site
     misc_feature    6655..6676
                     /label=BioBricks prefix
BASE COUNT     1811 a   1445 c   1463 g   1957 t
ORIGIN      
        1 tggtgcaaaa cctttgcggt atggcatgat agcgcctact agagaaagag gagaaatact
       61 agatgtccag attagataaa agtaaagtga ttaacagcgc attagagctg cttaatgagg
      121 tcggaatcga aggtttaaca acccgtaaac tcgcccagaa gctaggtgta gagcagccta
      181 cattgtattg gcatgtaaaa aataagcggg ctttgctcga cgccttagcc attgagatgt
      241 tagataggca ccatactcac ttttgccctt tagaagggga aagctggcaa gattttttac
      301 gtaataacgc taaaagtttt agatgtgctt tactaagtca tcgcgatgga gcaaaagtac
      361 atttaggtac acggcctaca gaaaaacagt atgaaactct cgaaaatcaa ttagcctttt
      421 tatgccaaca aggtttttca ctagagaatg cattatatgc actcagcgct gtggggcatt
      481 ttactttagg ttgcgtattg gaagatcaag agcatcaagt cgctaaagaa gaaagggaaa
      541 cacctactac tgatagtatg ccgccattat tacgacaagc tatcgaatta tttgatcacc
      601 aaggtgcaga gccagccttc ttattcggcc ttgaattgat catatgcgga ttagaaaaac
      661 aacttaaatg tgaaagtggg tcctaataat actagagcca ggcatcaaat aaaacgaaag
      721 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
      781 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agagtcccta
      841 tcagtgatag agattgacat ccctatcagt gatagagata ctgagcacta ctagagaaag
      901 aggagaaata ctagatgatt actcatggtt gttatacccg gacccggcac aagcataagc
      961 taaaaaaaac attgattatg cttagtgctg gtttaggatt gtttttttat gttaatcaga
     1021 actcatttgc aaatggtgaa aattatttta aattgggttc ggattcaaaa ctgttaactc
     1081 atgatagcta tcagaatcgc cttttttata cgttgaaaac tggtgaaact gttgccgatc
     1141 tttctaaatc gcaagatatt aatttatcga cgatttggtc gttgaataag catttataca
     1201 gttctgaaag cgaaatgatg aaggccgcgc ctggtcagca gatcattttg ccactcaaaa
     1261 aacttccctt tgaatacagt gcactaccac ttttaggttc ggcacctctt gttgctgcgg
     1321 gtggtgttgc tggtcacacg aataaactga ctaaaatgtc cccggacgtg accaaaagca
     1381 acatgaccga tgacaaggca ttaaattatg cggcacaaca ggcggcgagt ctcggtagcc
     1441 agcttcagtc gcgatctctg aacggcgatt acgcgaaaga taccgctctt ggtatcgctg
     1501 gtaaccaggc ttcgtcacag ttgcaggcct ggttacaaca ttatggaacg gcagaggtta
     1561 atctgcaaag tggtaataac tttgacggta gttcactgga cttcttatta ccgttctatg
     1621 attccgaaaa aatgctggca tttggtcagg tcggagcgcg ttacattgac tcccgcttta
     1681 cggcaaattt aggtgcgggt cagcgttttt tccttcctgc aaacatgttg ggctataacg
     1741 tcttcattga tcaggatttt tctggtgata atacccgttt aggtattggt ggcgaatact
     1801 ggcgagacta tttcaaaagt agcgttaacg gctatttccg catgagcggc tggcatgagt
     1861 catacaataa gaaagactat gatgagcgcc cagcaaatgg cttcgatatc cgttttaatg
     1921 gctatctacc gtcatatccg gcattaggcg ccaagctgat atatgagcag tattatggtg
     1981 ataatgttgc tttgtttaat tctgataagc tgcaatcgaa tcctggtgcg gcgaccgttg
     2041 gtgtaaacta tactccgatt cctctggtga cgatggggat cgattaccgt catggtacgg
     2101 gtaatgaaaa tgatctcctt tactcaatgc agttccgtta tcagtttgat aaatcgtggt
     2161 ctcagcaaat tgaaccacag tatgttaacg agttaagaac attatcaggc agccgttacg
     2221 atctggttca gcgtaataac aatattattc tggagtacaa gaagcaggat attctttctc
     2281 tgaatattcc gcatgatatt aatggtactg aacacagtac gcagaagatt cagttgatcg
     2341 ttaagagcaa atacggtctg gatcgtatcg tctgggatga tagtgcatta cgcagtcagg
     2401 gcggtcagat tcagcatagc ggaagccaaa gcgcacaaga ctaccaggct attttgcctg
     2461 cttatgtgca aggtggcagc aatatttata aagtgacggc tcgcgcctat gaccgtaatg
     2521 gcaatagctc taacaatgta cagcttacta ttaccgttct gtcgaatggt caagttgtcg
     2581 accaggttgg ggtaacggac tttacggcgg ataagacttc ggctaaagcg gataacgccg
     2641 ataccattac ttataccgcg acggtgaaaa agaatggggt agctcaggct aatgtccctg
     2701 tttcatttaa tattgtttca ggaactgcaa ctcttggggc aaatagtgcc aaaacggatg
     2761 ctaacggtaa ggcaaccgta acgttgaagt cgagtacgcc aggacaggtc gtcgtgtctg
     2821 ctaaaaccgc ggagatgact tcagcactta atgccagtgc ggttatattt tttgatggtg
     2881 cgactagaat ggaggaaccc caatccgatc cttccgtaga gccgccttta tcacaggaga
     2941 cgttctcgga cctttggaaa ttgttacccg agaataatgt tctgtcaccg ttgccctcac
     3001 aagctatgga tgacttaatg ttgtctccag atgatattga acaatggttt acagaggacc
     3061 ccggtccaga tgaagctccg cgcatgccag aagctgcacc ccgtgtcgct cccgctccag
     3121 cagcaccaac tccagcagcg ccagcacctg ccccttcttg gccattgtcg tcttccgtac
     3181 ctagtcagaa aacttaataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg
     3241 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
     3301 actggctcac cttcgggtgg gcctttctgc gtttatatac tagtagcggc cgctgcagtc
     3361 cggcaaaaaa acgggcaagg tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt
     3421 acttcgcgtt atgcaggctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     3481 gcgagcggta tcagctcact caaaggcggt aatctcgagg ttacattgtc gatctgttca
     3541 tggtgaacag ctttaaatgc accaaaaact cgtaaaagct ctgatgtatc tatctttttt
     3601 acaccgtttt catctgtgca tatggacagt tttccctttg atatctaacg gtgaacagtt
     3661 gttctacttt tgtttgttag tcttgatgct tcactgatag atacaagagc cataagaacc
     3721 tcagatcctt ccgtatttag ccagtatgtt ctctagtgtg gttcgttgtt tttgcgtgag
     3781 ccatgagaac gaaccattga gatcatgctt actttgcatg tcactcaaaa attttgcctc
     3841 aaaactggtg agctgaattt ttgcagttaa agcatcgtgt agtgtttttc ttagtccgtt
     3901 acgtaggtag gaatctgatg taatggttgt tggtattttg tcaccattca tttttatctg
     3961 gttgttctca agttcggtta cgagatccat ttgtctatct agttcaactt ggaaaatcaa
     4021 cgtatcagtc gggcggcctc gcttatcaac caccaatttc atattgctgt aagtgtttaa
     4081 atctttactt attggtttca aaacccattg gttaagcctt ttaaactcat ggtagttatt
     4141 ttcaagcatt aacatgaact taaattcatc aaggctaatc tctatatttg ccttgtgagt
     4201 tttcttttgt gttagttctt ttaataacca ctcataaatc ctcatagagt atttgttttc
     4261 aaaagactta acatgttcca gattatattt tatgaatttt tttaactgga aaagataagg
     4321 caatatctct tcactaaaaa ctaattctaa tttttcgctt gagaacttgg catagtttgt
     4381 ccactggaaa atctcaaagc ctttaaccaa aggattcctg atttccacag ttctcgtcat
     4441 cagctctctg gttgctttag ctaatacacc ataagcattt tccctactga tgttcatcat
     4501 ctgagcgtat tggttataag tgaacgatac cgtccgttct ttccttgtag ggttttcaat
     4561 cgtggggttg agtagtgcca cacagcataa aattagcttg gtttcatgct ccgttaagtc
     4621 atagcgacta atcgctagtt catttgcttt gaaaacaact aattcagaca tacatctcaa
     4681 ttggtctagg tgattttaat cactatacca attgagatgg gctagtcaat gataattaca
     4741 tgtccttttc ctttgagttg tgggtatctg taaattctgc tagacctttg ctggaaaact
     4801 tgtaaattct gctagaccct ctgtaaattc cgctagacct ttgtgtgttt tttttgttta
     4861 tattcaagtg gttataattt atagaataaa gaaagaataa aaaaagataa aaagaataga
     4921 tcccagccct gtgtataact cactacttta gtcagttccg cagtattaca aaaggatgtc
     4981 gcaaacgctg tttgctcctc tacaaaacag accttaaaac cctaaaggct taagtagcac
     5041 cctcgcaagc tcgggcaaat cgctgaatat tccttttgtc tccgaccatc aggcacctga
     5101 gtcgctgtct ttttcgtgac attcagttcg ctgcgctcac ggctctggca gtgaatgggg
     5161 gtaaatggca ctacaggcgc cttttatgga ttcatgcaag gaaactaccc ataatacaag
     5221 aaaagcccgt cacgggcttc tcagggcgtt ttatggcggg tctgctatgt ggtgctatct
     5281 gactttttgc tgttcagcag ttcctgccct ctgattttcc agtctgacca cttcggatta
     5341 tcccgtgaca ggtcattcag actggctaat gcacccagta aggcagcggt atcatcaaca
     5401 ggcttacccg tcttactgtc cctagtgctt ggattctcac caataaaaaa cgcccggcgg
     5461 caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg atctatcaac
     5521 aggagtccaa gcgagctcgt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     5581 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     5641 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     5701 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     5761 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     5821 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     5881 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     5941 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     6001 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     6061 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     6121 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     6181 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     6241 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     6301 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     6361 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     6421 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     6481 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc ccatggtgcc
     6541 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     6601 gaggcagaat ttcagataaa aaaaatcctt agctttcgct aaggatgatt tctggaattc
     6661 gcggccgctt ctagag
//
LOCUS       pDSG287                 6382 bp    DNA     circular SYN 14-JUN-2018
DEFINITION  Synthetic plasmid.
ACCESSION   pDSG287
VERSION
KEYWORDS    .
SOURCE      Synthetic plasmid
  ORGANISM  Synthetic plasmid
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 6382)
  AUTHORS   Glass,D.S. and Riedel-Kruse,I.H.
  TITLE     A synthetic bacterial cell-cell adhesion toolbox for programming
            multicellular morphologies and patterns
  JOURNAL   Cell (2018) In press
REFERENCE   2  (bases 1 to 6382)
  AUTHORS   Glass,D.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2018) Bioengineering, Stanford University, 318
            Campus Drive, Clark Center E350, Stanford, CA 94305, USA
COMMENT     Bankit Comment: ALT EMAIL:dsglass@gmail.com
            Bankit Comment: Vecscreen Comment:Submitter says that this sequence
            represents a Cloning Vector
            Bankit Comment: TOTAL # OF SEQS:83.
FEATURES             Location/Qualifiers
     source          1..6382
                     /organism="Synthetic plasmid"
                     /mol_type="other DNA"
                     /db_xref="taxon:1440148"
     misc_feature    1..689
                     /label=BBa_I732084
     misc_feature    1..36
                     /label=pLacIQ (BBa_I4032, 1nt deletion)
     misc_feature    45..56
                     /label=BBa_J04500(1)
     misc_feature    45..56
                     /label=RBS (BBa_B0034)(1)
     CDS             63..686
                     /codon_start=1
                     /transl_table=11
                     /product="tetR ORF (BBa_I732080)"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     misc_feature    698..826
                     /label=Double terminator (BBa_B0015)
     misc_feature    835..896
                     /label=BBa_J13002
     misc_feature    835..888
                     /label=pTet (BBa_R0040)
     misc_feature    897..908
                     /label=BBa_J04500
     misc_feature    897..908
                     /label=RBS (BBa_B0034)
     CDS             915..2882
                     /codon_start=1
                     /transl_table=11
                     /product="Neae_removedRestrictionsites"
                     /translation="MITHGCYTRTRHKHKLKKTLIMLSAGLGLFFYVNQNSFANGENY
                     FKLGSDSKLLTHDSYQNRLFYTLKTGETVADLSKSQDINLSTIWSLNKHLYSSESEMM
                     KAAPGQQIILPLKKLPFEYSALPLLGSAPLVAAGGVAGHTNKLTKMSPDVTKSNMTDD
                     KALNYAAQQAASLGSQLQSRSLNGDYAKDTALGIAGNQASSQLQAWLQHYGTAEVNLQ
                     SGNNFDGSSLDFLLPFYDSEKMLAFGQVGARYIDSRFTANLGAGQRFFLPANMLGYNV
                     FIDQDFSGDNTRLGIGGEYWRDYFKSSVNGYFRMSGWHESYNKKDYDERPANGFDIRF
                     NGYLPSYPALGAKLIYEQYYGDNVALFNSDKLQSNPGAATVGVNYTPIPLVTMGIDYR
                     HGTGNENDLLYSMQFRYQFDKSWSQQIEPQYVNELRTLSGSRYDLVQRNNNIILEYKK
                     QDILSLNIPHDINGTEHSTQKIQLIVKSKYGLDRIVWDDSALRSQGGQIQHSGSQSAQ
                     DYQAILPAYVQGGSNIYKVTARAYDRNGNSSNNVQLTITVLSNGQVVDQVGVTDFTAD
                     KTSAKADNADTITYTATVKKNGVAQANVPVSFNIVSGTATLGANSAKTDANGKATVTL
                     KSSTPGQVVVSAKTAEMTSALNASAVIFFDGA"
     CDS             2889..2900
                     /codon_start=1
                     /transl_table=11
                     /product="EPEA peptide (C-termial of alpha synuclein)"
                     /translation="-PEA"
     misc_feature    2901..2906
                     /label=Stop codons
     regulatory      2915..3043
                     /regulatory_class="terminator"
                     /label=terminator B0015
     misc_feature    3044..3064
                     /label=biobrick suffix
     misc_feature    3065..3138
                     /label=his operon terminator
     misc_feature    complement(3202..3219)
                     /label=VR primer site
     CDS             complement(3427..4377)
                     /codon_start=1
                     /transl_table=11
                     /product="repA"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRK
                     ERTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAK
                     FSSEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHK
                     ANIEISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTL
                     IFQVELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTS
                     FEAKFLSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     misc_feature    complement(4381..4393)
                     /label=RBS for repA
     regulatory      complement(4398..4417)
                     /regulatory_class="promoter"
                     /label=repA promoter
     CDS             complement(5263..6123)
                     /codon_start=1
                     /transl_table=11
                     /product="AmpR"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     misc_feature    complement(6124..6202)
                     /label=Promoter/RBS for AmpR
     misc_feature    6243..6263
                     /label=VF2 primer site
     misc_feature    6361..6382
                     /label=BioBricks prefix
BASE COUNT     1744 a   1352 c   1400 g   1886 t
ORIGIN      
        1 tggtgcaaaa cctttgcggt atggcatgat agcgcctact agagaaagag gagaaatact
       61 agatgtccag attagataaa agtaaagtga ttaacagcgc attagagctg cttaatgagg
      121 tcggaatcga aggtttaaca acccgtaaac tcgcccagaa gctaggtgta gagcagccta
      181 cattgtattg gcatgtaaaa aataagcggg ctttgctcga cgccttagcc attgagatgt
      241 tagataggca ccatactcac ttttgccctt tagaagggga aagctggcaa gattttttac
      301 gtaataacgc taaaagtttt agatgtgctt tactaagtca tcgcgatgga gcaaaagtac
      361 atttaggtac acggcctaca gaaaaacagt atgaaactct cgaaaatcaa ttagcctttt
      421 tatgccaaca aggtttttca ctagagaatg cattatatgc actcagcgct gtggggcatt
      481 ttactttagg ttgcgtattg gaagatcaag agcatcaagt cgctaaagaa gaaagggaaa
      541 cacctactac tgatagtatg ccgccattat tacgacaagc tatcgaatta tttgatcacc
      601 aaggtgcaga gccagccttc ttattcggcc ttgaattgat catatgcgga ttagaaaaac
      661 aacttaaatg tgaaagtggg tcctaataat actagagcca ggcatcaaat aaaacgaaag
      721 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
      781 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agagtcccta
      841 tcagtgatag agattgacat ccctatcagt gatagagata ctgagcacta ctagagaaag
      901 aggagaaata ctagatgatt actcatggtt gttatacccg gacccggcac aagcataagc
      961 taaaaaaaac attgattatg cttagtgctg gtttaggatt gtttttttat gttaatcaga
     1021 actcatttgc aaatggtgaa aattatttta aattgggttc ggattcaaaa ctgttaactc
     1081 atgatagcta tcagaatcgc cttttttata cgttgaaaac tggtgaaact gttgccgatc
     1141 tttctaaatc gcaagatatt aatttatcga cgatttggtc gttgaataag catttataca
     1201 gttctgaaag cgaaatgatg aaggccgcgc ctggtcagca gatcattttg ccactcaaaa
     1261 aacttccctt tgaatacagt gcactaccac ttttaggttc ggcacctctt gttgctgcgg
     1321 gtggtgttgc tggtcacacg aataaactga ctaaaatgtc cccggacgtg accaaaagca
     1381 acatgaccga tgacaaggca ttaaattatg cggcacaaca ggcggcgagt ctcggtagcc
     1441 agcttcagtc gcgatctctg aacggcgatt acgcgaaaga taccgctctt ggtatcgctg
     1501 gtaaccaggc ttcgtcacag ttgcaggcct ggttacaaca ttatggaacg gcagaggtta
     1561 atctgcaaag tggtaataac tttgacggta gttcactgga cttcttatta ccgttctatg
     1621 attccgaaaa aatgctggca tttggtcagg tcggagcgcg ttacattgac tcccgcttta
     1681 cggcaaattt aggtgcgggt cagcgttttt tccttcctgc aaacatgttg ggctataacg
     1741 tcttcattga tcaggatttt tctggtgata atacccgttt aggtattggt ggcgaatact
     1801 ggcgagacta tttcaaaagt agcgttaacg gctatttccg catgagcggc tggcatgagt
     1861 catacaataa gaaagactat gatgagcgcc cagcaaatgg cttcgatatc cgttttaatg
     1921 gctatctacc gtcatatccg gcattaggcg ccaagctgat atatgagcag tattatggtg
     1981 ataatgttgc tttgtttaat tctgataagc tgcaatcgaa tcctggtgcg gcgaccgttg
     2041 gtgtaaacta tactccgatt cctctggtga cgatggggat cgattaccgt catggtacgg
     2101 gtaatgaaaa tgatctcctt tactcaatgc agttccgtta tcagtttgat aaatcgtggt
     2161 ctcagcaaat tgaaccacag tatgttaacg agttaagaac attatcaggc agccgttacg
     2221 atctggttca gcgtaataac aatattattc tggagtacaa gaagcaggat attctttctc
     2281 tgaatattcc gcatgatatt aatggtactg aacacagtac gcagaagatt cagttgatcg
     2341 ttaagagcaa atacggtctg gatcgtatcg tctgggatga tagtgcatta cgcagtcagg
     2401 gcggtcagat tcagcatagc ggaagccaaa gcgcacaaga ctaccaggct attttgcctg
     2461 cttatgtgca aggtggcagc aatatttata aagtgacggc tcgcgcctat gaccgtaatg
     2521 gcaatagctc taacaatgta cagcttacta ttaccgttct gtcgaatggt caagttgtcg
     2581 accaggttgg ggtaacggac tttacggcgg ataagacttc ggctaaagcg gataacgccg
     2641 ataccattac ttataccgcg acggtgaaaa agaatggggt agctcaggct aatgtccctg
     2701 tttcatttaa tattgtttca ggaactgcaa ctcttggggc aaatagtgcc aaaacggatg
     2761 ctaacggtaa ggcaaccgta acgttgaagt cgagtacgcc aggacaggtc gtcgtgtctg
     2821 ctaaaaccgc ggagatgact tcagcactta atgccagtgc ggttatattt tttgatggtg
     2881 cgactagaga accagaggct taataatact agagccaggc atcaaataaa acgaaaggct
     2941 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
     3001 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactagt agcggccgct
     3061 gcagtccggc aaaaaaacgg gcaaggtgtc accaccctgc cctttttctt taaaaccgaa
     3121 aagattactt cgcgttatgc aggcttcctc gctcactgac tcgctgcgct cggtcgttcg
     3181 gctgcggcga gcggtatcag ctcactcaaa ggcggtaatc tcgaggttac attgtcgatc
     3241 tgttcatggt gaacagcttt aaatgcacca aaaactcgta aaagctctga tgtatctatc
     3301 ttttttacac cgttttcatc tgtgcatatg gacagttttc cctttgatat ctaacggtga
     3361 acagttgttc tacttttgtt tgttagtctt gatgcttcac tgatagatac aagagccata
     3421 agaacctcag atccttccgt atttagccag tatgttctct agtgtggttc gttgtttttg
     3481 cgtgagccat gagaacgaac cattgagatc atgcttactt tgcatgtcac tcaaaaattt
     3541 tgcctcaaaa ctggtgagct gaatttttgc agttaaagca tcgtgtagtg tttttcttag
     3601 tccgttacgt aggtaggaat ctgatgtaat ggttgttggt attttgtcac cattcatttt
     3661 tatctggttg ttctcaagtt cggttacgag atccatttgt ctatctagtt caacttggaa
     3721 aatcaacgta tcagtcgggc ggcctcgctt atcaaccacc aatttcatat tgctgtaagt
     3781 gtttaaatct ttacttattg gtttcaaaac ccattggtta agccttttaa actcatggta
     3841 gttattttca agcattaaca tgaacttaaa ttcatcaagg ctaatctcta tatttgcctt
     3901 gtgagttttc ttttgtgtta gttcttttaa taaccactca taaatcctca tagagtattt
     3961 gttttcaaaa gacttaacat gttccagatt atattttatg aattttttta actggaaaag
     4021 ataaggcaat atctcttcac taaaaactaa ttctaatttt tcgcttgaga acttggcata
     4081 gtttgtccac tggaaaatct caaagccttt aaccaaagga ttcctgattt ccacagttct
     4141 cgtcatcagc tctctggttg ctttagctaa tacaccataa gcattttccc tactgatgtt
     4201 catcatctga gcgtattggt tataagtgaa cgataccgtc cgttctttcc ttgtagggtt
     4261 ttcaatcgtg gggttgagta gtgccacaca gcataaaatt agcttggttt catgctccgt
     4321 taagtcatag cgactaatcg ctagttcatt tgctttgaaa acaactaatt cagacataca
     4381 tctcaattgg tctaggtgat tttaatcact ataccaattg agatgggcta gtcaatgata
     4441 attacatgtc cttttccttt gagttgtggg tatctgtaaa ttctgctaga cctttgctgg
     4501 aaaacttgta aattctgcta gaccctctgt aaattccgct agacctttgt gtgttttttt
     4561 tgtttatatt caagtggtta taatttatag aataaagaaa gaataaaaaa agataaaaag
     4621 aatagatccc agccctgtgt ataactcact actttagtca gttccgcagt attacaaaag
     4681 gatgtcgcaa acgctgtttg ctcctctaca aaacagacct taaaacccta aaggcttaag
     4741 tagcaccctc gcaagctcgg gcaaatcgct gaatattcct tttgtctccg accatcaggc
     4801 acctgagtcg ctgtcttttt cgtgacattc agttcgctgc gctcacggct ctggcagtga
     4861 atgggggtaa atggcactac aggcgccttt tatggattca tgcaaggaaa ctacccataa
     4921 tacaagaaaa gcccgtcacg ggcttctcag ggcgttttat ggcgggtctg ctatgtggtg
     4981 ctatctgact ttttgctgtt cagcagttcc tgccctctga ttttccagtc tgaccacttc
     5041 ggattatccc gtgacaggtc attcagactg gctaatgcac ccagtaaggc agcggtatca
     5101 tcaacaggct tacccgtctt actgtcccta gtgcttggat tctcaccaat aaaaaacgcc
     5161 cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct
     5221 atcaacagga gtccaagcga gctcgtaaac ttggtctgac agttaccaat gcttaatcag
     5281 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     5341 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     5401 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     5461 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     5521 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     5581 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     5641 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     5701 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     5761 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     5821 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     5881 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     5941 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     6001 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     6061 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     6121 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     6181 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttcccat
     6241 ggtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     6301 tatcacgagg cagaatttca gataaaaaaa atccttagct ttcgctaagg atgatttctg
     6361 gaattcgcgg ccgcttctag ag
//
LOCUS       pDSG312                 6342 bp    DNA     circular SYN 14-JUN-2018
DEFINITION  Synthetic plasmid.
ACCESSION   pDSG312
VERSION
KEYWORDS    .
SOURCE      Synthetic plasmid
  ORGANISM  Synthetic plasmid
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 6342)
  AUTHORS   Glass,D.S. and Riedel-Kruse,I.H.
  TITLE     A synthetic bacterial cell-cell adhesion toolbox for programming
            multicellular morphologies and patterns
  JOURNAL   Cell (2018) In press
REFERENCE   2  (bases 1 to 6342)
  AUTHORS   Glass,D.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2018) Bioengineering, Stanford University, 318
            Campus Drive, Clark Center E350, Stanford, CA 94305, USA
COMMENT     Bankit Comment: ALT EMAIL:dsglass@gmail.com
            Bankit Comment: Vecscreen Comment:Submitter says that this sequence
            represents a Cloning Vector
            Bankit Comment: TOTAL # OF SEQS:83.
FEATURES             Location/Qualifiers
     source          1..6342
                     /organism="Synthetic plasmid"
                     /mol_type="other DNA"
                     /db_xref="taxon:1440148"
     misc_feature    1..1210
                     /label=I0500
     misc_feature    complement(1..1079)
                     /label=AraC
     misc_feature    complement(1043..1114)
                     /label=PC
     misc_feature    1080..1210
                     /label=pBAD
     misc_feature    1219..1230
                     /label=BBa_J04500
     misc_feature    1219..1230
                     /label=RBS (BBa_B0034)
     CDS             1237..3204
                     /codon_start=1
                     /transl_table=11
                     /product="Neae_removedRestrictionsites"
                     /translation="MITHGCYTRTRHKHKLKKTLIMLSAGLGLFFYVNQNSFANGENY
                     FKLGSDSKLLTHDSYQNRLFYTLKTGETVADLSKSQDINLSTIWSLNKHLYSSESEMM
                     KAAPGQQIILPLKKLPFEYSALPLLGSAPLVAAGGVAGHTNKLTKMSPDVTKSNMTDD
                     KALNYAAQQAASLGSQLQSRSLNGDYAKDTALGIAGNQASSQLQAWLQHYGTAEVNLQ
                     SGNNFDGSSLDFLLPFYDSEKMLAFGQVGARYIDSRFTANLGAGQRFFLPANMLGYNV
                     FIDQDFSGDNTRLGIGGEYWRDYFKSSVNGYFRMSGWHESYNKKDYDERPANGFDIRF
                     NGYLPSYPALGAKLIYEQYYGDNVALFNSDKLQSNPGAATVGVNYTPIPLVTMGIDYR
                     HGTGNENDLLYSMQFRYQFDKSWSQQIEPQYVNELRTLSGSRYDLVQRNNNIILEYKK
                     QDILSLNIPHDINGTEHSTQKIQLIVKSKYGLDRIVWDDSALRSQGGQIQHSGSQSAQ
                     DYQAILPAYVQGGSNIYKVTARAYDRNGNSSNNVQLTITVLSNGQVVDQVGVTDFTAD
                     KTSAKADNADTITYTATVKKNGVAQANVPVSFNIVSGTATLGANSAKTDANGKATVTL
                     KSSTPGQVVVSAKTAEMTSALNASAVIFFDGA"
     CDS             3211..3588
                     /codon_start=1
                     /transl_table=11
                     /product="Nanobody against EPEA tag"
                     /translation="-
                     GQLVESGGGSVQAGGSLRLSCAASGIDSSSYCMGWFRQRPGKEREGVARINGLGGVKT
                     AYADSVKDRFTISRDNAENTVYLQMNSLKPEDTAIYYCAAKFSPGYCGGSWSNFGYWG
                     QGTQVTVSS"
     misc_feature    3589..3594
                     /label=Stop codons
     misc_feature    3595..3615
                     /label=biobrick suffix
     misc_feature    3616..3687
                     /label=his operon terminator
     misc_feature    complement(3751..3768)
                     /label=VR primer site
     CDS             complement(3831..4646)
                     /codon_start=1
                     /transl_table=11
                     /product="KanR"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     misc_feature    complement(4978..5802)
                     /label=p15A ORI
     misc_feature    6203..6223
                     /label=VF2 primer site
     misc_feature    6321..6342
                     /label=biobrick prefix
BASE COUNT     1706 a   1497 c   1483 g   1656 t
ORIGIN      
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagaa agaggagaaa tactagatga ttactcatgg ttgttatacc
     1261 cggacccggc acaagcataa gctaaaaaaa acattgatta tgcttagtgc tggtttagga
     1321 ttgttttttt atgttaatca gaactcattt gcaaatggtg aaaattattt taaattgggt
     1381 tcggattcaa aactgttaac tcatgatagc tatcagaatc gcctttttta tacgttgaaa
     1441 actggtgaaa ctgttgccga tctttctaaa tcgcaagata ttaatttatc gacgatttgg
     1501 tcgttgaata agcatttata cagttctgaa agcgaaatga tgaaggccgc gcctggtcag
     1561 cagatcattt tgccactcaa aaaacttccc tttgaataca gtgcactacc acttttaggt
     1621 tcggcacctc ttgttgctgc gggtggtgtt gctggtcaca cgaataaact gactaaaatg
     1681 tccccggacg tgaccaaaag caacatgacc gatgacaagg cattaaatta tgcggcacaa
     1741 caggcggcga gtctcggtag ccagcttcag tcgcgatctc tgaacggcga ttacgcgaaa
     1801 gataccgctc ttggtatcgc tggtaaccag gcttcgtcac agttgcaggc ctggttacaa
     1861 cattatggaa cggcagaggt taatctgcaa agtggtaata actttgacgg tagttcactg
     1921 gacttcttat taccgttcta tgattccgaa aaaatgctgg catttggtca ggtcggagcg
     1981 cgttacattg actcccgctt tacggcaaat ttaggtgcgg gtcagcgttt tttccttcct
     2041 gcaaacatgt tgggctataa cgtcttcatt gatcaggatt tttctggtga taatacccgt
     2101 ttaggtattg gtggcgaata ctggcgagac tatttcaaaa gtagcgttaa cggctatttc
     2161 cgcatgagcg gctggcatga gtcatacaat aagaaagact atgatgagcg cccagcaaat
     2221 ggcttcgata tccgttttaa tggctatcta ccgtcatatc cggcattagg cgccaagctg
     2281 atatatgagc agtattatgg tgataatgtt gctttgttta attctgataa gctgcaatcg
     2341 aatcctggtg cggcgaccgt tggtgtaaac tatactccga ttcctctggt gacgatgggg
     2401 atcgattacc gtcatggtac gggtaatgaa aatgatctcc tttactcaat gcagttccgt
     2461 tatcagtttg ataaatcgtg gtctcagcaa attgaaccac agtatgttaa cgagttaaga
     2521 acattatcag gcagccgtta cgatctggtt cagcgtaata acaatattat tctggagtac
     2581 aagaagcagg atattctttc tctgaatatt ccgcatgata ttaatggtac tgaacacagt
     2641 acgcagaaga ttcagttgat cgttaagagc aaatacggtc tggatcgtat cgtctgggat
     2701 gatagtgcat tacgcagtca gggcggtcag attcagcata gcggaagcca aagcgcacaa
     2761 gactaccagg ctattttgcc tgcttatgtg caaggtggca gcaatattta taaagtgacg
     2821 gctcgcgcct atgaccgtaa tggcaatagc tctaacaatg tacagcttac tattaccgtt
     2881 ctgtcgaatg gtcaagttgt cgaccaggtt ggggtaacgg actttacggc ggataagact
     2941 tcggctaaag cggataacgc cgataccatt acttataccg cgacggtgaa aaagaatggg
     3001 gtagctcagg ctaatgtccc tgtttcattt aatattgttt caggaactgc aactcttggg
     3061 gcaaatagtg ccaaaacgga tgctaacggt aaggcaaccg taacgttgaa gtcgagtacg
     3121 ccaggacagg tcgtcgtgtc tgctaaaacc gcggagatga cttcagcact taatgccagt
     3181 gcggttatat tttttgatgg tgcgactaga caagggcagc ttgttgagag cgggggggga
     3241 agcgtacaag ccgggggatc gctgcgcttg tcgtgtgcag cttccggtat tgattccagc
     3301 tcatactgta tgggttggtt tcgtcagcgt ccgggaaagg agcgcgaggg tgtcgcacgt
     3361 atcaacgggt tgggcggcgt taaaacagct tacgctgact ctgttaaaga tcgtttcacc
     3421 attagtcgtg ataatgcaga aaatactgtt tatttgcaaa tgaactcctt gaaacccgaa
     3481 gatacagcta tttattattg tgctgccaag tttagtccag gttattgcgg aggatcgtgg
     3541 agcaatttcg gatactgggg tcaaggaacg caagtgaccg tatcttcgta ataatactag
     3601 tagcggccgc tgcagtccgg caaaaaaggg caaggtgtca ccaccctgcc ctttttcttt
     3661 aaaaccgaaa agattacttc gcgttatgca ggcttcctcg ctcactgact cgctgcgctc
     3721 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatct cgagtcccgt
     3781 caagtcagcg taatgctctg ccagtgttac aaccaattaa ccaattctga ttagaaaaac
     3841 tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt
     3901 tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca
     3961 agatcctggt atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc
     4021 ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt
     4081 gagaatggca aaagcttatg catttctttc cagacttgtt caacaggcca gccattacgc
     4141 tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg
     4201 agacgaaata cgcgatcgct gttaaaagga caattacaaa caggaatcga atgcaaccgg
     4261 cgcaggaaca ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat
     4321 acctggaatg ctgttttccc ggggatcgca gtggtgagta accatgcatc atcaggagta
     4381 cggataaaat gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc
     4441 atctcatctg taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc
     4501 gcatcgggct tcccatacaa tcgatagatt gtcgcacctg attgcccgac attatcgcga
     4561 gcccatttat acccatataa atcagcatcc atgttggaat ttaatcgcgg cctcgagcaa
     4621 gacgtttccc gttgaatatg gctcataaca ccccttgtat tactgtttat gtaagcagac
     4681 agttttattg ttcatgatga tatattttta tcttgtgcaa tgtaacatca gagattttga
     4741 gacacaacgt ggctttgttg aataaatcga acttttgctg agttgaagga tcagatcacg
     4801 catcttcccg acaacgcaga ccgttccgtg gcaaagcaaa agttcaaaat caccaactgg
     4861 tccacctaca acaaagctct catcaaccgt ggctccctca ctttctggct ggatgatggg
     4921 gcgattcagg cctggtatga gtcagcaaca ccttcttcac gaggcagacc tcagcgctag
     4981 cggagtgtat actggcttac tatgttggca ctgatgaggg tgtcagtgaa gtgcttcatg
     5041 tggcaggaga aaaaaggctg caccggtgcg tcagcagaat atgtgataca ggatatattc
     5101 cgcttcctcg ctcactgact cgctacgctc ggtcgttcga ctgcggcgag cggaaatggc
     5161 ttacgaacgg ggcggagatt tcctggaaga tgccaggaag atacttaaca gggaagtgag
     5221 agggccgcgg caaagccgtt tttccatagg ctccgccccc ctgacaagca tcacgaaatc
     5281 tgacgctcaa atcagtggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     5341 ctggcggctc cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt gtcattccgc
     5401 tgttatggcc gcgtttgtct cattccacgc ctgacactca gttccgggta ggcagttcgc
     5461 tccaagctgg actgtatgca cgaacccccc gttcagtccg accgctgcgc cttatccggt
     5521 aactatcgtc ttgagtccaa cccggaaaga catgcaaaag caccactggc agcagccact
     5581 ggtaattgat ttagaggagt tagtcttgaa gtcatgcgcc ggttaaggct aaactgaaag
     5641 gacaagtttt ggtgactgcg ctcctccaag ccagttacct cggttcaaag agttggtagc
     5701 tcagagaacc ttcgaaaaac cgccctgcaa ggcggttttt tcgttttcag agcaagagat
     5761 tacgcgcaga ccaaaacgat ctcaagaaga tcatcttatt aaggggtctg acgctcagtg
     5821 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     5881 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     5941 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     6001 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     6061 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     6121 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     6181 ctccatccag tctattccat ggtgccacct gacgtctaag aaaccattat tatcatgaca
     6241 ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa atccttagct
     6301 ttcgctaagg atgatttctg gaattcgcgg ccgcttctag ag
//
